Loss of genetic variability in the captive stocks of tambaqui,Colossoma macropomum (Cuvier, 1818), at breeding centres in Brazil,and their divergence from wild populations          下载免费PDF全文

Jonas da Paz Aguiar  Paola Fabiana Fazzi Gomes  Igor Guerreiro Hamoy  Sidney Emanuel Batista dos Santos  Horacio Schneider  Iracilda Sampaio 《Aquaculture Research》2018,49(5):1914-1925

The loss of variability in farmed populations and the risks of interactions with wild populations support the need for the genetic monitoring of species farmed throughout the world. In Brazil, the tambaqui is the most widely farmed native fish species. Despite this, there are no data on the pedigree of the farmed stocks, and the potential for interactions with wild populations in the Amazon basin has raised concerns with regard to the genetic variability of these stocks. The present study analysed sequences of the mitochondrial Control Region and 12 microsatellites to characterize the genetic variability of seven historically important commercial tambaqui breeding centres located in four different regions of Brazil, and compared these sequences with those obtained from individuals collected from a wild population. High levels of genetic diversity were found in the wild population, whereas genetic diversity was reduced in both markers in most captive populations, except for the broodstock located near the Amazon River. High F_ST and D_EST indices were recorded between the wild population and most of the captive stocks analysed. The drastic reduction in genetic diversity found in most captive stocks and the difference between these stocks and the wild population may have been the result of the small size of the founding populations and the absence of breeding management. The renewal of the broodstocks and the application of breeding management techniques are recommended. In the Amazon region, in addition, the use of broodstocks that are genetically very different from local wild populations should be avoided.  相似文献   

Soybean seed coat chitinase as a defense protein against the stored product pest Callosobruchus maculatus          下载免费PDF全文

Nadia CM Silva  Jamile G Conceição  Kayan Eudorico Ventury  Leonardo FR De Sá  Eduardo AG Oliveira  Izabela S Santos  Valdirene M Gomes  Monique N Costa  Andre TS Ferreira  Jonas Perales  Jose Xavier‐Filho  Kátia VS Fernandes  Antonia EA Oliveira 《Pest management science》2018,74(6):1449-1456

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Binding of imidacloprid,thiamethoxam and N‐desmethylthiamethoxam to nicotinic receptors of Myzus persicae: pharmacological profiling using neonicotinoids,natural agonists and antagonists          下载免费PDF全文

Hartmut Kayser  Katrin Lehmann  Marilyne Gomes  Wolfgang Schleicher  Karin Dotzauer  Margarethe Moron  Peter Maienfisch 《Pest management science》2016,72(11):2166-2175

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A multi-environment trials diallel analysis provides insights on the inheritance of fumonisin contamination resistance in tropical maize     

José Maria Villela Pádua  Kaio Olímpio Das Graças Dias  Maria Marta Pastina  João Cândido de Souza  Valéria Aparecida Vieira Queiroz  Rodrigo Veras da Costa  Maria Beatriz Pereira da Silva  Carlos Alexandre Gomes Ribeiro  Claudia Teixeira Guimarães  Salvador Alejandro Gezan  Lauro José Moreira Guimarães 《Euphytica》2016,211(3):277-285

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Can Some Marine-Derived Fungal Metabolites Become Actual Anticancer Agents?     

Nelson G. M. Gomes  Florence Lefranc  Anake Kijjoa  Robert Kiss 《Marine drugs》2015,13(6):3950-3991

Marine fungi are known to produce structurally unique secondary metabolites, and more than 1000 marine fungal-derived metabolites have already been reported. Despite the absence of marine fungal-derived metabolites in the current clinical pipeline, dozens of them have been classified as potential chemotherapy candidates because of their anticancer activity. Over the last decade, several comprehensive reviews have covered the potential anticancer activity of marine fungal-derived metabolites. However, these reviews consider the term “cytotoxicity” to be synonymous with “anticancer agent”, which is not actually true. Indeed, a cytotoxic compound is by definition a poisonous compound. To become a potential anticancer agent, a cytotoxic compound must at least display (i) selectivity between normal and cancer cells (ii) activity against multidrug-resistant (MDR) cancer cells; and (iii) a preferentially non-apoptotic cell death mechanism, as it is now well known that a high proportion of cancer cells that resist chemotherapy are in fact apoptosis-resistant cancer cells against which pro-apoptotic drugs have more than limited efficacy. The present review thus focuses on the cytotoxic marine fungal-derived metabolites whose ability to kill cancer cells has been reported in the literature. Particular attention is paid to the compounds that kill cancer cells through non-apoptotic cell death mechanisms.  相似文献   

Phenotyping Castanea hybrids for Phytophthora cinnamomi resistance          下载免费PDF全文

C. Santos  H. Machado  I. Correia  F. Gomes  J. Gomes‐Laranjo  R. Costa 《Plant pathology》2015,64(4):901-910

Castanea sativa is susceptible to Phytophthora spp., a serious root pathogen causing ink disease, while C. crenata and C. mollissima show resistance to infection. Interspecific controlled crosses were established for introgression of resistance genes from the resistant species into the susceptible C. sativa, and two mapping populations were created. Phytophthora cinnamomi resistance of each progeny was evaluated by root and excised shoot inoculation tests. The number of days of survival after root inoculation was the best discriminator of resistance to P. cinnamomi while the percentage of shoots with internal lesions was the symptom most associated with survival. The lesion progression rate in the excised shoot inoculation test was strongly and negatively correlated with survival in the root inoculation test. The excised shoot inoculation test appears to be a reliable approach for screening the resistance of chestnut genotypes to P. cinnamomi. Strong genetic correlations were obtained between survival and ink disease symptoms and among symptoms, indicating that common or linked genes might influence resistance to P. cinnamomi. The most resistant genotypes selected from this study will be tested for other commercial variables, such as ease of vegetative propagation and stock–scion compatibility.  相似文献   

Methods for Cercospora coffeicola protoplast isolation and genetic transformation with the green fluorescent protein     

André Gomes Coelho Souza  Sonia Herrero  Luiz Antonio Maffia  Margaret Elizabeth Daub 《European journal of plant pathology / European Foundation for Plant Pathology》2014,139(2):235-238

Cercospora coffeicola is the causal agent of brown eye spot on coffee leaves. Although the disease has significant importance, few molecular studies have been done with C. coffeicola. Here we report a protocol for isolating protoplasts as well as development of a genetic transformation system using Green Fluorescent Protein. High yields of protoplasts (≈10⁸/ml) were obtained from mycelial cultures from five isolates of C. coffeicola. One isolate was transformed with a vector encoding hygromycin resistance and Green Fluorescent Protein. Out of 43 hygromycin-resistant transformants obtained, Green Fluorescent Protein was highly expressed in one (2.3 %).  相似文献   

Parasitological diagnosis of canine visceral leishmaniasis: Is intact skin a good target?     

M.F. Madeira  F.B. Figueiredo  A.G.S. Pinto  L.D. Nascimento  M. Furtado  E. Mouta-Confort  C.C. de Paula  A. Bogio  M.C.A. Gomes  A.M.S. Bessa  S.R.L. Passos 《Research in veterinary science》2009,87(2):260-262

The objective of this study was to evaluate intact skin of seroreactive dogs as a possible target for the parasitological confirmation of canine visceral leishmaniasis (CVL). For this purpose, 394 dogs identified in serological surveys carried out in the metropolitan region of Belo Horizonte were studied. Blood was collected from all animals for serology and a tissue sample was obtained from two sites for parasitological diagnosis. Skin obtained from the ear and scapular region was simultaneously analyzed in 247 animals and lesion samples and ear skin were analyzed in 147 dogs. Leishmania parasites were isolated from 310 (78.7%) animals, and all isolates were identified as Leishmania chagasi. Simultaneous isolation from two sites was possible in 240 of the 310 animals, including ear and scapular skin in 151/247 (61.1%) and ear skin and skin lesions in 89/147 (60.5%). Ours results suggest that intact skin is one of the main target sites for the parasitological confirmation of CVL in seroreactive dogs.  相似文献   

Primary Hyperparathyroidism and Monoclonal Gammopathy in a Dog     

G. Benchekroun  A. Desmyter  A. Hidalgo  H.-J. Boulouis  E. Gomes  F. Garnier  L. Escoffier  L. Poujol  P. de Fornel-Thibaud  C. Maurey  D. Rosenberg 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2009,23(1):211-214

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Effects of single and mixed-diatom diets on growth,condition, and survival of larvae of the sea urchin Paracentrotus lividus (Lamarck, 1816)     

Gomes  Ana  Lourenço  Sílvia  Santos  Pedro M.  Raposo  Andreia  Mendes  Susana  Gonçalves  Sílvia C.  Ferreira  Susana M. F.  Pombo  Ana 《Aquaculture International》2021,29(3):1069-1090

Aquaculture International - The development of rearing protocols promoting the larval development, pre and post-metamorphosis are key for echinoculture. Mixed diets combining diatom with other...  相似文献