全文获取类型
收费全文 | 192篇 |
免费 | 43篇 |
专业分类
林业 | 24篇 |
农学 | 1篇 |
2篇 | |
综合类 | 21篇 |
农作物 | 2篇 |
水产渔业 | 7篇 |
畜牧兽医 | 172篇 |
园艺 | 1篇 |
植物保护 | 5篇 |
出版年
2022年 | 1篇 |
2021年 | 1篇 |
2020年 | 2篇 |
2018年 | 3篇 |
2017年 | 1篇 |
2016年 | 1篇 |
2015年 | 7篇 |
2014年 | 4篇 |
2013年 | 14篇 |
2012年 | 6篇 |
2011年 | 8篇 |
2010年 | 8篇 |
2009年 | 9篇 |
2008年 | 9篇 |
2007年 | 8篇 |
2006年 | 5篇 |
2005年 | 8篇 |
2004年 | 3篇 |
2003年 | 12篇 |
2002年 | 8篇 |
2001年 | 9篇 |
2000年 | 6篇 |
1999年 | 11篇 |
1998年 | 9篇 |
1997年 | 5篇 |
1996年 | 7篇 |
1995年 | 7篇 |
1994年 | 5篇 |
1993年 | 5篇 |
1992年 | 7篇 |
1991年 | 7篇 |
1990年 | 7篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1986年 | 4篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 1篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1973年 | 1篇 |
1970年 | 3篇 |
1966年 | 1篇 |
排序方式: 共有235条查询结果,搜索用时 78 毫秒
131.
We present prediction models for estimating tree mortality resulting from gypsy moth, Lymantria dispar, defoliation in mixed oak, Quercus sp., forests. These models differ from previous work by including defoliation as a factor in the analysis. Defoliation intensity, initial tree crown condition (crown vigour), crown position, and species grouping classes were highly significant in categorical analysis of variance for mortality. Heavy defoliation intensity was shown to have a strong, consistent influence in increasing the probability of tree mortality. Classification and Regression Tree (CART) analysis, a binomial decision tree procedure, was used to develop prediction models of mortality risk for use by forest managers. The best decision tree had 65 groups that correctly classified 75% of the live trees and 76% of the dead trees. Models were run separately by defoliation class and provided correct classifications between 63 and 78% of the trees. Forest land managers can use these models to assign probabilities of death for moderate and heavy defoliation intensity levels and compare predicted mortality to mortality of undefoliated trees to determine how gypsy moth defoliation will affect their stands. The probabilities can be used to develop marking guides Lased on projected defoliation levels for implementing silvicultural treatments to minimize gypsy moth effects in forest stands prior to infestation. 相似文献
132.
TH Jones LJ Thompson JH Lawton TM Bezemer RD Bardgett TM Blackburn KD Bruce PF Cannon GS Hall SE Hartley G Howson CG Jones C Kampichler E Kandeler DA Ritchie 《Science (New York, N.Y.)》1998,280(5362):441-443
In model terrestrial ecosystems maintained for three plant generations at elevated concentrations of atmospheric carbon dioxide, increases in photosynthetically fixed carbon were allocated below ground, raising concentrations of dissolved organic carbon in soil. These effects were then transmitted up the decomposer food chain. Soil microbial biomass was unaffected, but the composition of soil fungal species changed, with increases in rates of cellulose decomposition. There were also changes in the abundance and species composition of Collembola, fungal-feeding arthropods. These results have implications for long-term feedback processes in soil ecosystems that are subject to rising global atmospheric carbon dioxide concentrations. 相似文献
133.
Sonia Lacouture Yaindrys Rodriguez Olivera Segura Mariela Marcelo Gottschalk 《Canadian journal of veterinary research》2022,86(1):78
Streptococcus suis is one of the most important swine bacterial pathogens causing economic losses. This report presents the serotype distribution of S. suis recovered from diseased pigs in Québec from January 2015 to June 2020. Serotypes 1/2 and 2 predominated, followed by serotypes 7, 3, 5, 4, 9, 1, and 14. Compared to previously reported data, very few changes could be observed concerning the serotype distribution, indicating a relative stability. Half of the untypable isolates did not belong to the species S. suis sensu stricto, as determined by recN polymerase chain reaction. Less than 10% of “real S. suis” isolates were untypable. The genetic diversity of S. suis serotypes 1, 2, and 14, as analyzed by multilocus sequence typing, was mainly represented by sequence type (ST)1, ST28, ST25, and ST94. All ST1 isolates (considered highly virulent) belonged to either serotype 1 or 14. 相似文献
134.
Genomic relatedness among Actinobacillus pleuropneumoniae field strains of sterotypes 1 and 5 isolated from healthy and diseased pigs. 下载免费PDF全文
S Chatellier J Harel D Dugourd B Chevallier M Kobisch M Gottschalk 《Canadian journal of veterinary research》1999,63(3):170-176
Forty-four Actinobacillus pleuropneumoniae isolates recovered from both healthy and diseased pigs were characterized by random amplified polymorphic DNA analysis (RAPD), pulsed field gel electrophoresis (PFGE) and apx toxin gene typing. Nine RAPD types and 14 PFGE patterns were identified. No common RAPD or PFGE patterns were found between strains of serotype 1 and those of serotype 5. The RAPD analysis indicated that the 15 serotype 1 strains isolated from diseased pigs were assigned to 4 RAPD types, with 66% of strains characterized by the same RAPD type. By contrast, the 5 strains of serotype 1 isolated from healthy carriers were dispersed in 4 RAPD types. These data suggest that the diversity of strains isolated from healthy pigs could be higher than that of strains recovered from diseased pigs. In addition, all serotype 5 strains exhibited a unique RAPD type. Unlike RAPD, PFGE analysis allowed discrimination among isolates of serotype 1 and among those of serotype 5. All but 3 isolates showed the same apx genotype as their respective serotype reference strain. These data indicate that RAPD analysis is a valuable rapid tool for routine subtyping of strains of serotype 1. For strains of serotype 5, a combination of several typing methods, such as PFGE and apx gene typing, is needed to provide useful information on the molecular epidemiology of swine pleuropneumonia. 相似文献
135.
M Gottschalk A Lebrun S Lacouture J Harel C Forget K R Mittal 《Journal of veterinary diagnostic investigation》2000,12(5):444-449
In the present study, the characterization of 3 atypical isolates of Actinobacillus pleuropneumoniae is presented. Two isolates (1B and 27E) showed positive reactions in coagglutination, immunodiffusion, and indirect hemagglutination tests for serotypes 1 and 7, whereas the third isolate (26B) reacted with antisera to serotypes 1, 4, and 7. These atypical isolates of A. pleuropneumoniae possessed a capsular polysaccharide (CPS) antigenically related to serotype 1 as well as an O-chain lipopolysaccharide antigenically related to serotype 7 or to serotypes 4 and 7, as shown by the use of monoclonal antibodies. Results of toxin profile and virulence assays for mice and pigs showed them to be more related to A. pleuropneumoniae serotype 7 field isolates. All 3 isolates induced antibodies mainly against serotype 7/4 O-long-chain lipopolysaccharide (LC-LPS) and, to a lesser extent, to the CPS of serotype 1, in experimentally infected pigs. Diagnostic laboratories that use a LC-LPS-based enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of A. pleuropneumoniae infection in swine would probably diagnose herds infected with these atypical isolates as being infected by A. pleuropneumoniae serotypes 7 or 4, whereas those that use a CPS-based ELISA would probably consider them as infected by A. pleuropneumoniae serotype 1. 相似文献
136.
DE Boerner RD Kurtz JA Craven GM Ross FW Jones WJ Davis 《Science (New York, N.Y.)》1999,283(5402):668-670
The subcrustal lithosphere underlying the southern Archean Churchill Province (ACP) in western Canada is at least one order of magnitude more electrically conductive than the lithosphere beneath adjacent Paleoproterozoic crust. The measured electrical properties of the lithosphere underlying most of the Paleoproterozoic crust can be explained by the conductivity of olivine. Mantle xenolith and geological mapping evidence indicate that the lithosphere beneath the southern ACP was substantially modified as a result of being trapped between two nearly synchronous Paleoproterozoic subduction zones. Tectonically induced metasomatism thus may have enhanced the subcrustal lithosphere conductivity of the southern ACP. 相似文献
137.
138.
Perry MB Angen Ø MacLean LL Lacouture S Kokotovic B Gottschalk M 《Veterinary microbiology》2012,156(3-4):403-410
Atypical Actinobacillus pleuropneumoniae serotype 13 strains present in North America are described here for the first time. Different from serotype 13 strains described in Europe, North America strains are biotype I and antigenically related to both, serotypes 13 and 10. Chemical and structural analysis of the capsular polysaccharide (CPS) and lipopolysaccharide (LPS) of a representative strain revealed that the CPS is almost identical to that of the reference strain of serotype 13, having a slightly higher degree of glycose O-acetylation. However, it produces an O-PS within the LPS antigenically and structurally identical with that of the reference strain of A. pleuropneumoniae serotype 10. The O-PS was characterized as a homopolymer of 1,2 linked β-D-galactofuranosyl residues, a structure unrelated to that of the O-PS produced by the reference strain of serotype 13. Strains from Canada and United States are antigenically, phenotypically and genotypically similar. Animals infected by one of these strains induced antibodies that were detected by a LPS-based ELISA diagnostic test using either the homologous antigen or that of serotype 10. Based on the LPS and toxin profile, these strains might be misidentified as A. pleuropneumoniae serotype 10. 相似文献
139.
140.
Taíssa Cook Siqueira Soares Marcelo Gottschalk Sonia Lacouture Jane Megid Paulo Eduardo Martins Ribolla José Carlos de Figueiredo Pantoja Antonio Carlos Paes 《Canadian journal of veterinary research》2015,79(4):279-284
Streptococcus suis is an important pathogen in the swine industry. This article is the first to report the occurrence, risk factors, serotype distribution, and antimicrobial susceptibility of S. suis recovered from employees and environmental samples of swine slaughterhouses in Brazil. Tonsillar swabs from all 139 pig-slaughtering employees and 261 environmental swabs were collected for detection of S. suis and serotyping by monoplex and multiplex polymerase chain reaction, respectively. Antimicrobial susceptibility was determined by the disk-diffusion method. Although S. suis was not detected in any of the tested employees, it was isolated from 25% of the environmental samples. Significant differences (P < 0.05) in the occurrence of S. suis were observed between slaughterhouses and between areas of low, medium, and high risk. The most frequent serotypes were 4 and 29, each accounting for 12% of the isolates, followed by 5, 12, 21, and 31, each accounting for 6%. High rates of susceptibility to the antimicrobials doxycycline (100%), ceftiofur (94%), ampicillin (81%), and cephalexin (75%) were observed. However, multidrug resistance was observed in all the isolates. Because S. suis is present in the environment of swine slaughterhouses, on carcasses and knives, as well as on the hands of employees in all areas, all employees are at risk of infection. 相似文献