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71.
72.
Fairs and petting zoos have been associated with outbreaks of zoonotic disease. Previously, the presence of meticillin-resistant Staphylococcus aureus (MRSA) was documented in commercial pigs; therefore, it was hypothesised that antibiotic-resistant S aureus may also occur in pigs exhibited at agricultural fairs. To test this hypothesis, 157 pigs were swabbed at two state fairs in 2008 to 2009. Both nares were sampled and cultures were grown in enrichment broth, then plated onto selective MRSA plates and blood plates. S aureus was confirmed using phenotypic and molecular methods, and was analysed using spa typing, gene-specific polymerase chain reaction and antibiotic susceptibility testing. The presence of S aureus was confirmed in samples collected from pigs exhibited at USA pig shows. Twenty-five of 157 (15.9 per cent) samples were positive for S aureus. Two isolates (8 per cent) were resistant to meticillin; 23/25 (92 per cent), 14/25 (56 per cent) and 15/25 (60 per cent) were resistant to tetracycline, erythromycin and clindamycin, respectively. spa typing revealed multiple isolates of spa type t034 (9/25, 36 per cent) and t337 (7/25, 28 per cent) and singletons of t002, t209, t526, t1236, t1334, t1683, t3075, t5784 and t5883. These results verify the presence of antibiotic-resistant S aureus in pigs exhibited at USA fairs, suggesting that pigs are a potential reservoir for S aureus within this environment.  相似文献   
73.
Reasons for performing study: Lyophilised products from green‐lipped mussel (Perna canaliculus[LPPC]) are used to orally treat horses with osteoarthritis (OA). However, no randomised, controlled or double‐blinded studies on the efficacy of this treatment in horses have been reported to date. Objective: To investigate the effects of a unique LPPC (Biolane) 1 in improving clinical signs of OA in the fetlock. Methods: Data were analysed from 26 horses with primary fetlock lameness in a controlled, randomised and double‐blinded, multi‐centre clinical trial. The study design was a partial crossover with a washout period and consisted of 19 horses treated with LPPC and 20 with a placebo. Horses were dosed orally with 25 mg/kg bwt/day LPPC or placebo for 56 days. Efficacy was evaluated by clinical assessment of lameness, passive flexion, pain, swelling and heat in the affected joint. Relationships between variables were analysed using an ordinal logistic model with random effects for horse and horse x treatment according to a modified intention‐to‐treat analysis. Results: Clinical evaluation of horses with a fetlock lameness treated with LPPC showed a significant reduction in severity of lameness (P<0.001), improved response to the joint flexion test (P<0.001) and reduced joint pain (P = 0.014) when compared with horses treated with placebo. Conclusions: The LPPC significantly alleviated the severity of lameness and joint pain and improved response to joint flexion in horses with lameness attributable to OA in the fetlock.  相似文献   
74.
Hyperlipidemia in Caspian miniature horses: Effects of undernutrition   总被引:1,自引:0,他引:1  
The susceptibility of Caspian miniature horses to hyperlipidemia was followed in a study of healthy Caspian miniature horses in which food but not water was withheld for 48 hours. Two months before the study, the horses were fed a high-energy diet. The mean of weight gain was 26.46 kg per horse during this period. All horses had a normal rectal temperature, respiratory rate, and pulse throughout the experiment. During the period of food deprivation, levels of serum triglyceride, aspartate aminotransferase, and alanine aminotransferase significantly (P < .05) increased and glucose concentration decreased. As a conclusion, Caspian miniature horses were susceptible to hyperlipidemia, but they did not show classic hyperlipemia syndrome.  相似文献   
75.
Abstract

Digltaria eriantha and Chloris gayana were grown under controlled conditions for three months and were treated with a nutrient solution containing 150 mMol NaCl and the following nitrogen sources: 25 or 200 mg/l NH4 +‐N or NO3 ?‐N or no nitrogen. The application of nitrogen was found to stimulate growth, i.e. leaf area and dry mass in both grasses, with a greatest growth response to both NH4 +—N treatments in D. eriantha, and NH4 +‐N and NO3 ?—N treatments in C. gayana. Proline accumulated in both grasses, but this accumulation followed different trends in the two grasses. Soluble sugars (non‐structural) accumulated in the above ground component in D. eriantha, while in C. gayana soluble sugars accumulated predominantly in the roots, possibly as osmotica, or for storage and may thus have been available for regrowth.  相似文献   
76.
Purposeful introductions of exotic species for rehabilitation efforts following wildfire are common on rangelands in the western United States, though ecological impacts of exotic species in novel environments are often poorly understood. One such introduced species, Kochia prostrata (L.) Schrad (forage kochia) has been seeded on over 200 000 ha throughout the Intermountain West to provide fuel breaks and forage, and to compete with invasive plants. Despite its potential benefits, K. prostrata has been reported to spread from some seeded areas, and no studies have addressed its potential interactions with native species. A systematic investigation is needed to increase understanding of the extent to which K. prostrata spreads from seeded areas, the environmental conditions under which it spreads, and its interactions with the associated plant communities. We sampled 28 K. prostrata postfire rehabilitation and greenstrip seedings in southwestern Idaho, which ranged from 3 to 24 yr since seeding. We analyzed cover of K. prostrata and the associated plant community in adjacent seeded and unseeded areas, and quantified extent of spread from seeded areas. Abundance of K. prostrata was negatively associated with that of most plant functional groups, including native species, but was positively associated with abundance of exotic annual forbs. Kochia prostrata spread to unseeded areas on 89% of sampled sites; distances of the farthest individual from the seeding boundary were greater than those previously reported, ranging from 0 to 710 m, with a mean distance of 208 m. Further, although the area covered by K. prostrata increased with time since seeding, we found no evidence that plant community composition affected spread of K. prostrata. Results contribute to current understanding of potential ecological implications of seeding K. prostrata and will enhance the ability of land managers to make scientifically based management decisions about its use.  相似文献   
77.
This paper provides an example of the practical application of multi-attribute trade-off analysis (MATA) to wildfire management. The MATA approach supports more informed decision-making because it exposes important trade-offs among competing management objectives (requiring value-based choices), helps guide and structure necessary technical judgements, explicitly represents uncertainty (i.e., not just expected outcomes but risk profiles around outcomes) and addresses temporal trade-offs. MATA promotes critical thinking about what analysis is required for decision-making. A MATA approach can be applied for all types of forest and fire management decisions. In this paper, we provide a sample application of MATA to an evaluation of landscape-level fuel treatments for managing wildfire risk. The study area is located in southeastern British Columbia, Canada where historical fire suppression policies and expanding development in wildland urban interface areas have resulted in an increase in both the probability and the consequences of stand replacement fires. We specify management objectives and develop measurable attributes for fire management costs, timber supply, property damage, landscape-level biodiversity, local air quality and climate change. We then simulate the effects on these attributes of four alternative fuel management strategies that include combinations of mechanical treatments and prescribed burning over a 100-year period. The evaluation illustrates the key features of MATA while highlighting the benefits and challenges of implementing the approach.  相似文献   
78.
The objective of this study was to describe the distribution of Salmonella spp. on Ontario grower-finisher pig farms. Eighty swine farms were visited from January through July 2004. On each farm, fecal samples were collected from 5 pens, 2 rectal samples and 1 pooled sample from fresh manure on the floor per pen. Salmonella was isolated from 91 (11%) of the 800 rectal samples and 73 (18%) of the 397 pooled samples. Overall, Salmonella was recovered from 37 (46%) of the 80 farms. On each positive farm, Salmonella was cultured from 1 to 7 pigs or 1 to 5 pens. Of the 37 farms, 18, 13, 5, and 1 yielded 1, 2, 3, and 4 serovars, respectively. The most common serovars were S. Typhimurium var. Copenhagen, S. Infantis, S. Typhimurium, S. Derby, S. Agona, S. Havana, and S. enterica subsp. I:Rough-O. The 3 most frequent phage types were PT 104, PT 104a, and PT 104b. There was a statistically fair agreement between samples collected directly from pigs and pooled pen samples in determining the Salmonella status at the pen and farm level (kappa = 0.6, P < 0.0001). However, in 62 pens, Salmonella status, serovars, or phage types differed between the pig and pooled pen samples. The distribution of Salmonella on the swine farms in this study indicates that, in developing an intervention strategy, priority should be given to farms positive for S. Typhimurium var. Copenhagen. Also, the variation in Salmonella status between pig and pooled pen samples deserves consideration in a sampling strategy.  相似文献   
79.
Creation of a reliable and easy to use serologic test would greatly improve ante mortem diagnosis of Mycobacterium avium subsp. avium and aid in the control of avian mycobacteriosis, particularly in captive birds. In order to determine whether serodiagnostics could be of value in testing ring-neck doves (Streptopelia risoria) for M. a. avium infection, Western blot analysis was used to assess the humoral response of ring-neck doves exposed to M. a. avium, and to evaluate whether an association could be made between the humoral response and necropsy findings, histopathology, culture, and PCR testing. Western blot results were examined for reactivity patterns associating humoral response with infection status, severity and type of lesions (diffuse vs. multifocal granulomatous inflammation) and phenotype (white vs. non-white). A sensitivity of 88.24% and a specificity of 100% were achieved utilizing Western blot analysis to detect M. a. avium infection in ring-neck doves, offering a negative predictive value of 93% and a positive predictive value of 100%. While Western blot analysis results did not reflect lesion severity, lesion type did partially correspond with the humoral response. The findings of the present study indicate that serologic testing can be used as a valuable ante mortem screening tool for identifying ring-neck doves infected with M. a. avium.  相似文献   
80.
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