Elucidation of the small RNA component of the transcriptome   总被引：3，自引：0，他引：3  

Lu C  Tej SS  Luo S  Haudenschild CD  Meyers BC  Green PJ 《Science (New York, N.Y.)》2005,309(5740):1567-1569

Small RNAs play important regulatory roles in most eukaryotes, but only a small proportion of these molecules have been identified. We sequenced more than two million small RNAs from seedlings and the inflorescence of the model plant Arabidopsis thaliana. Known and new microRNAs (miRNAs) were among the most abundant of the nonredundant set of more than 75,000 sequences, whereas more than half represented lower abundance small interfering RNAs (siRNAs) that match repetitive sequences, intergenic regions, and genes. Individual or clusters of highly regulated small RNAs were readily observed. Targets of antisense RNA or miRNA did not appear to be preferentially associated with siRNAs. Many genomic regions previously considered featureless were found to be sites of numerous small RNAs.  相似文献   

台湾青枣设施栽培表现与发展前景初探     

夏国京  张力飞  钟明英  胡凤兰 《辽宁农业职业技术学院学报》2002,4(4):28-30

辽宁省设施果树生产,由于存在气候、资源、技术等方面的优势,栽培面积、产量和经济效益在逐渐扩大和提高.但从树种、品种结构上看,南方的树种几乎是一个空白.台湾青枣的引进,并在设施栽培上取得一定的成功,无疑打破了辽宁果树设施栽培中只有落叶果树树种的局面,但由于诸多因素的限制,如设施结构保温性能差,技术不到位,果实品质较差,风味淡,商品化程度较低等因素,制约了其发展.从当前果树发展形势来看,台湾青枣应适当栽植,限量发展.  相似文献   

Initiating and cancer-propagating cells in TEL-AML1-associated childhood leukemia     

Hong D  Gupta R  Ancliff P  Atzberger A  Brown J  Soneji S  Green J  Colman S  Piacibello W  Buckle V  Tsuzuki S  Greaves M  Enver T 《Science (New York, N.Y.)》2008,319(5861):336-339

Understanding cancer pathogenesis requires knowledge of not only the specific contributory genetic mutations but also the cellular framework in which they arise and function. Here we explore the clonal evolution of a form of childhood precursor-B cell acute lymphoblastic leukemia that is characterized by a chromosomal translocation generating a TEL-AML1 fusion gene. We identify a cell compartment in leukemic children that can propagate leukemia when transplanted in mice. By studying a monochorionic twin pair, one preleukemic and one with frank leukemia, we establish the lineal relationship between these "cancer-propagating" cells and the preleukemic cell in which the TEL-AML1 fusion first arises or has functional impact. Analysis of TEL-AML1-transduced cord blood cells suggests that TEL-AML1 functions as a first-hit mutation by endowing this preleukemic cell with altered self-renewal and survival properties.  相似文献   

An RNA processing activity that debranches RNA lariats   总被引：68，自引：0，他引：68  

B Ruskin  M R Green 《Science (New York, N.Y.)》1985,229(4709):135-140

The excised introns of pre-messenger RNA's (pre-mRNA's) and intron-containing splicing intermediates are in a lariat configuration in which the 5' end of the intron is covalently joined by a 2',5'-phosphodiester bond to a specific adenosine residue near the 3' end of the intron. A 2',5'-phosphodiesterase activity in HeLa cell extracts has been detected that debranches RNA lariats, converting them to linear RNA molecules by specific cleavage of the 2',5'-phosphodiester bond. This lariat debranching activity is distinct from previously reported 2',5'-phosphodiesterases with regard to its biochemical and substrate requirements as well as its stringent cleavage specificity. The debranching activity is observed only if the RNA lariats generated during in vitro processing are deproteinized and added back to the extract. These results suggest that during the normal in vitro splicing reaction the 2',5'-phosphodiester bond of RNA lariats is protected from cleavage by the lariat debranching activity.  相似文献   

Molecular basis of carcinogenesis by human adenoviruses     

Green M  Piña M  Fujinaga K 《Science (New York, N.Y.)》1967,158(3800):528

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UNIVERSITY AND EDUCATIONAL NOTES     

Green RG 《Science (New York, N.Y.)》1925,62(1597):133-134

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Group Research     

Green DE 《Science (New York, N.Y.)》1954,119(3092):444-445

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Earth's Bow Shock: Elapsed-Time Observations by Two Closely Spaced Satellites     

Greenstadt EW  Green IM  Colburn DS 《Science (New York, N.Y.)》1968,162(3856):898-901

Coordinated observations of the earth's bow shock were made as Vela 3A and Explorer 33 passed within 6 earth radii of each other. Elapsed time measurements of shock motion give directly determined velocities in the range 1 to 10 kilometers per second and establish the existence of two regions, one of large amplitude magnetic "shock" oscillations and another of smaller, sunward, upstream oscillations. Each region is as thick as 1 earth radius, or more.  相似文献   

Checkerboards and color aftereffects     

Green MA  Corwin TR  Zemon V 《Science (New York, N.Y.)》1977,198(4313):208-209

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Women job applicants     

Green BR 《Science (New York, N.Y.)》1973,181(4099):496

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