IgG isotype profile is correlated with cardiomegaly in Beagle dogs infected with distinct Trypanosoma cruzi strains     

Guedes PM  Veloso VM  Gollob KJ  Afonso LC  Caldas IS  Vianna P  de Lana M  Chiari E  Bahia MT  Galvão LM 《Veterinary immunology and immunopathology》2008,124(1-2):163-168

A systematic study following infection by various strains of the protozoan parasite, Trypanosoma cruzi, and the simultaneous monitoring of the humoral immune response together with the elicited cellular response, could add greatly to our understanding of differences between strains of this important human pathogen. In that sense, acute and chronic infections with distinct T. cruzi strains (Y, Berenice-78 and ABC) in Beagle dogs were studied through a longitudinal evaluation of immunoglobulin G (IgG), IgG1 and IgG2 isotypes (by ELISA and flow cytometry (FC)), as well as measurements of peripheral blood mononuclear cell (PBMC) proliferation over a 100-week period, and their correlation with cardiomegaly. Our results show that infected animals presenting cardiomegaly showed lower or absent levels of IgG1 during the chronic phase of the infection, when compared to those that did not show an increase in heart weight. In that manner, our results suggest that IgG1 could be used as a marker for cardiac pathogenicity in Chagas disease.  相似文献   

Occurrence and genotype characterization of Giardia duodenalis in goat kids from the Canary Islands, Spain     

Ruiz A  Foronda P  González JF  Guedes A  Abreu-Acosta N  Molina JM  Valladares B 《Veterinary parasitology》2008,154(1-2):137-141

Giardia duodenalis (syn. Giardia lamblia, Giardia intestinalis) is a wide-spread intestinal protozoa of both humans and animals. Although giardiosis in goat is commonly asymptomatic, young kids may bear an enteric disease associated with persistent diarrhoea and delayed weight gain. In the present study we have analysed the occurrence of Giardia in 315 young goat kids (2–6 months old) from Gran Canaria Island (Spain) through visualization of faecal cysts. The identification of genotypes of G. duodenalis among the farms was attained by nested PCR of the triophosphate isomerase (TPI) and single PCR of β-giardin genes and subsequent sequencing. Positive samples were found in 42.2% of the animals and 95.5% of the farms. Goat faecal specimens were positive for only livestock-associated G. duodenalis assemblage E genotype for both TPI and β-giardin genes. The genetic analysis of these two loci revealed the presence of different haplotypes among the farms included in the survey and high homology with homologous genes from cattle and sheep. Altogether, the data presented here provide additional information to the prevalence and genetic characterization of Giardia isolates. The absence of assemblages A and B in this study suggests that zoonotic transmission of Giardia from goats could be of low epidemiological significance, although these findings should be validated in studies including other geographical areas, age groups and larger number of samples.  相似文献   

A Proposed Role for VEGF Isoforms in Sex-Specific Vasculature Development in the Gonad     

RC Bott  DT Clopton  AS Cupp 《Reproduction in domestic animals》2008,43(S2):310-316

Many scientists have expended efforts to determine what regulates development of an indifferent gonad into either a testis or ovary. Expression of Sry and upregulation of Sox9 are factors that initiate formation of the testis-specific pathway to allow for both sex-specific vasculature and seminiferous cord formation. Migration of mesonephric precursors of peritubular myoid cells and endothelial cells into the differentiating testis is a critical step in formation of both of these structures. Furthermore, these events appear to be initiated downstream from Sry expression. Sertoli cell secretion of growth factors acts to attract these mesonephric cells. One hypothesis is that a growth factor specific for these cell linages act in concert to coordinate migration of both peritubular and endothelial cells. A second hypothesis is that several growth factors stimulate migration and differentiation of mesonephric 'stem-like' cells to result in migration and differentiation into several different cell lineages. While the specific mechanism is unclear, several growth factors have been implicated in the initiation of mesonephric cell migration. This review will focus on the proposed mechanisms of a growth factor, Vascular Endothelial Growth Factor, and how different angiogenic and inhibitory isoforms from this single gene may aid in development of testis-specific vascular development.  相似文献   

Transversus abdominis plane block in ponies: a preliminary anatomical study     

Caroline F. Baldo  Daniel Almeida  Erin Wendt-Hornickle  Alonso Guedes 《Veterinary anaesthesia and analgesia》2018,45(3):392-396

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In vitro antimicrobial activity against equine Lawsonia intracellularis strains     

C. E. R. Pereira  T. P. Resende  E. Vasquez  L. Marshall-Lund  R. M. C. Guedes  C. J. Gebhart 《Equine veterinary journal》2019,51(5):665-668

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Pharmacokinetics and antinociceptive effects of the soluble epoxide hydrolase inhibitor t‐TUCB in horses with experimentally induced radiocarpal synovitis          下载免费PDF全文

A. G. P. Guedes  F. Aristizabal  A. Sole  A. Adedeji  R. Brosnan  H. Knych  J. Yang  S.‐H. Hwang  C. Morisseau  B. D. Hammock 《Journal of veterinary pharmacology and therapeutics》2018,41(2):230-238

This study determined the pharmacokinetics, antinociceptive, and anti‐inflammatory effects of the soluble epoxide hydrolase (sEH ) inhibitor t ‐TUCB (trans ‐4‐{4‐[3‐(4‐Trifluoromethoxy‐phenyl)‐ureido]‐cyclohexyloxy}‐benzoic acid) in horses with lipopolysaccharide (LPS )‐induced radiocarpal synovitis. A total of seven adult healthy mares (n  = 4–6/treatment) were administered 3 μg LPS into one radiocarpal joint and t ‐TUCB intravenously (i.v.) at 0 (control), 0.03, 0.1, 0.3, and 1 mg/kg in a blinded, randomized, crossover design with at least 3 weeks washout between. Two investigators independently assigned pain scores (at rest, walk and trot) and lameness scores before and up to 48 hr after t ‐TUCB /LPS . Responses to touching the joint skin to assess tactile allodynia, plasma, and synovial fluid (SF ) t ‐TUCB concentrations were determined before and up to 48 hr after t ‐TUCB /LPS . Blood and SF were collected for clinical laboratory evaluations before and up to 48 hr after t ‐TUCB /LPS . Areas under the curves of pain and lameness scores were calculated and compared between control and treatments. Data were analyzed using repeated measures ANOVA with Dunnett or Bonferroni post‐test. p  < .05 was considered significant. Data are mean ± SEM . Compared to control, pain, lameness, and tactile allodynia were significantly lower with 1 mg/kg t ‐TUCB , but not the other doses. For 0.1, 0.3, and 1 mg/kg t ‐TUCB treatments, plasma terminal half‐lives were 13 ± 3, 13 ± 0.5, and 24 ± 5 hr, and clearances were 68 ± 15, 48 ± 5, and 14 ± 1 ml hr^?1 kg^?1. The 1 mg/kg t ‐TUCB reached the SF at high concentrations. There were no important anti‐inflammatory effects. In conclusion, sEH inhibition with t ‐TUCB may provide analgesia in horses with inflammatory joint pain.  相似文献   

Further insights into the role of methional and phenylacetaldehyde in lager beer flavor stability   总被引：2，自引：0，他引：2  

Soares da Costa M  Gonçalves C  Ferreira A  Ibsen C  Guedes de Pinho P  Silva Ferreira AC 《Journal of agricultural and food chemistry》2004,52(26):7911-7917

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Designing artificial anterior cruciate ligaments based on novel fibrous structures     

J. Cruz  S. Rana  R. Fangueiro  R. Guedes 《Fibers and Polymers》2014,15(1):181-186

The present paper reports the development of novel braided structures using polyamide 6.6 fibers for application as artificial anterior cruciate ligaments (ACLs). The developed structures were circular braids, axially reinforced with either a number of core yarns or braided structures. Tensile behavior of these structures was characterized and the effects of number of axial yarns or braids and, the number of yarns used in the axial braids were thoroughly investigated. From the experimental results, it was observed that the braided structures with axial braids could mimic the load-elongation behaviour of native ACL. The average breaking extension and strain at toe region were 30 % and 4.3 % respectively, which are in the range of native ACLs. The maximum breaking force and stiffness achieved with 7 axial braids, each produced using 6 yarns were 274 N and 13.5 N/mm respectively and, both breaking force and stiffess showed linear increase with the number of axial braids as well as number of yarns used in the axial braids. Therefore, it is possible to design an artificial graft using these novel braided sturctures with mechanical properties similar to that of native ACLs, through adjustment of these structural parameters, as these braided structures have much smaller diameter (0.5 mm) than native ACLs (11 mm).  相似文献   

Synergism and unintended effects of the association between imidacloprid and sodium chloride (NaCl) on the management of Euschistus heros     

Hígor S Rodrigues  Khalid Haddi  Mateus O Campos  Noelio A Ferreira‐Filho  Raul Narciso C Guedes  Philip L Newland  Eugênio E Oliveira 《Pest management science》2021,77(1):417-424

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Tension pneumothorax secondary to nasojejunal feeding tube misplacement in a mechanically ventilated dog     

P Giordano  BM Kirby  RC Bennett  F Bernard 《Australian veterinary journal》2014,92(10):400-404

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