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101.
Apparent digestibility coefficients (ADCs) provide estimates of nutrient availability in feedstuffs and can be used as a tool for selecting ingredients to compound cost‐effective diets for fish. Extrusion process is one factor that could influence ADC values. Thus, a digestibility trial was undertaken to evaluate ADC of protein, energy, and dry matter, and apparent amino acid availability (AAAA) in five cereal grain products/by‐products (corn, wheat middlings, broken rice, rice bran, and sorghum) in compounded diets for Nile tilapia. Chromic oxide was used as an external inert marker. The digestibility values of energy and dry matter in ingredients, in decreasing order, were highest for broken rice (95.34 and 96.45%), sorghum (82.37 and 87.29%), corn (67.34 and 82.21%), rice bran (57.58 and 55.59%), and wheat middlings (48.94 and 45.88%). Protein digestibility value was higher for corn (72.86%) and the lowest value was observed for sorghum (56.77%), while broken rice, wheat middlings, and rice bran presented values ranging from 63.01 to 66.88%. AAAA values were higher for corn (70.05%) and the lowest methionine and cystine availability values were recorded for wheat middlings and broken rice, respectively. Broken rice appears as one of the most indicated ingredient for using in Nile tilapia diets because of its highest values of nutrient digestibility when compared to the other energetic sources. The use of this information should aid more precise and economic diet formulation for Nile tilapia.  相似文献   
102.
The effects of different diets (natural or pellets) on growth, survival, and moulting interval of juvenile spider crabs, weighing between 0.011–1.56 g and up to 17.6 mm in carapace length, were tested over a period of 90 days. During experiment I, five diets were tested: (1) frozen shrimp—Paleomonetes sp., (2) fresh mussels—Mytilus sp., (3) white fish fillets—Merlucius merlucius, (4) blue fish fillets—Sardina pilchardus, and (5) commercial crustacean pellets. Spider crabs fed fresh mussels grew larger (0.98 ± 0.69 g) and had higher growth rates (4.0 ± 0.7 %BWd−1) compared to the other four diets. The crabs fed shrimp pellet and frozen shrimp grew to intermediate sizes and were smaller than the ones fed fresh mussels, but they were larger than spider crabs fed either blue or white fish fillets (0.46 ± 0.63 and 0.26 ± 0.13 g, respectively) compared to the ones fed white fish fillets (0.12 ± 0.04) and blue fish fillets (0.04 ± 0.02 g). The spider crabs fed blue fish fillets only lasted until day 60 of the experiment, after this day none of the 20 fed this diet were left. During experiment II, two diets were tested: (1) white and blue fish fillets and (2) commercial fish pellet. There were no differences in growth both in weight or carapace length (2.9 ± 1.8 and 2.1 ± 1.5 g in weight, and 18.9 ± 5.0 and 17.7 ± 3.3 mm, respectively) at the end of the experiment. Similarly, there were no differences in growth rates in weight between the two diets (1.2 ± 0.4 and 0.9 ± 0.3 %BWd−1, respectively) or in carapace length (0.4 ± 0.1 and 0.4 ± 0.2 %BWd−1, respectively). Fresh mussel appears to be a very good diet to culture the early stages of this species, while shrimp pellets also deliver acceptable results. On the contrary, frozen shrimp, fish fillets either from blue or white species (much higher lipid content in the blue species), and fish pellets were found to be bad diets for the culture of the early stages of M. brachydactyla.  相似文献   
103.
Japanese farmers manage their irrigation water based on their past experiences and preferences, considering such factors as weather and available water (hereafter defined as empirical water management). They elaborately control the intake and drainage rates of their own paddy fields to maintain optimal ponding depths. But these well-managed systems will drastically change because of the decreasing number of farmers. Therefore, it is necessary to clarify if the optimal ponding depth will be maintained within the limits of traditionally-allowed water intake rate from the main river. The first objective of this study was the quantification of actual water use in the paddy fields, resulting from the farmers water management on the basis of their experience. The significance of the present water intake rate under empirical water management was studied for a paddy field command area of about 230 ha. Water intake rates and the water requirements of the whole area were investigated by measuring the flow rate at 17 points of irrigation and drainage canals. Characteristics of the farmers empirical water management were investigated by measuring the hourly changes in inflow and outflow rates for a sub-area using an automatic measurement system, and an inferential method of determining water management patterns for the paddy fields was proposed. The newly-proposed inferential method was introduced in the tank model, which expresses the characteristics of water management in the command area. The Shuffled Complex Evolution Algorithm (SCE-UA) method was used for optimizing the model parameters. It was proven that the model accuracy improved when the farmers empirical water management was taken into account. The optimal amount of water to be applied to the command area was quantified by the simulation. The second objective was to predict the effect of the decreasing number of farmers on future water use conditions. The simulated result indicates the difficulty of maintaining optimal ponding depth for the whole command area when the farmers empirical water management is not maintained. In other words, results indicated that efficient water use requires an automatic water management system or a new pipeline system to replace the farmers present empirical water management.  相似文献   
104.
Nutrients constrain the soil carbon cycle in tropical forests, but we lack knowledge on how these constraints vary within the soil microbial community. Here, we used in situ fertilization in a montane tropical forest and in two lowland tropical forests on contrasting soil types to test the principal hypothesis that there are different nutrient constraints to different groups of microorganisms during the decomposition of cellulose. We also tested the hypotheses that decomposers shift from nitrogen to phosphorus constraints from montane to lowland forests, respectively, and are further constrained by potassium and sodium deficiency in the western Amazon. Cellulose and nutrients (nitrogen, phosphorus, potassium, sodium, and combined) were added to soils in situ, and microbial growth on cellulose (phospholipid fatty acids and ergosterol) and respiration were measured. Microbial growth on cellulose after single nutrient additions was highest following nitrogen addition for fungi, suggesting nitrogen as the primary limiting nutrient for cellulose decomposition. This was observed at all sites, with no clear shift in nutrient constraints to decomposition between lowland and montane sites. We also observed positive respiration and fungal growth responses to sodium and potassium addition at one of the lowland sites. However, when phosphorus was added, and especially when added in combination with other nutrients, bacterial growth was highest, suggesting that bacteria out-compete fungi for nitrogen where phosphorus is abundant. In summary, nitrogen constrains fungal growth and cellulose decomposition in both lowland and montane tropical forest soils, but additional nutrients may also be of critical importance in determining the balance between fungal and bacterial decomposition of cellulose.  相似文献   
105.

Purpose

The low conductivity of sediments for mass and electron transport is the most severe limiting factor in sediment microbial fuel cells (SMFCs), so that sediment ameliorations yielded more remarkable effects than electrode improvements. The objective of this research was to enhance the electricity generation of SMFCs with amendments of biochar to freshwater sediments for conductivity enhancement.

Materials and methods

Laboratory-scale SMFCs were constructed and biochars were produced from coconut shells at different temperatures. Variations in the power output, electrode potential, internal resistance, total organic carbon (TOC) content, and microbial communities were measured.

Results and discussion

Amending with biochar reduced the charge transfer resistances of SMFCs and enriched the Firmicutes (mainly Fusibacter sp.) in the sediment, which improved the SMFC power generation by two- to tenfold and enhanced the TOC removal rate by 1.7- to fourfold relative to those without the amendment.

Conclusions

The results suggested that biochar amendment is a promising strategy to enhance SMFC power production, and the electrical conductivity of biochar should be considered important when interpreting the impact biochar has on the electrical performance of soil or freshwater sediment MFCs.
  相似文献   
106.
This article aims to determine the effect of certain covariates, such as season of kidding, parity, and time of kidding on the characteristics of the lactation curve of Saanen dairy goats. Characteristics investigated are peak milk yield, time of peak milk yield, total milk production, persistency, and the relationship between fat and protein in milk composition, as well as between lactation curves of the same animal in successive years. The analysis is carried out using a hierarchical Bayesian approach, together with Wood’s model, to model lactation. Posterior distributions of quantities of interest are obtained by means of the Markov chain Monte Carlo (MCMC) methods. These clearly illustrate the significant effect of especially parity, but also season and time of kidding on the characteristics of the lactation curve. Total and peak milk yield increase with increasing parity up to about the third or fourth parity, while peak yield is later for first than for later parities. The analysis also enables estimation of lactation characteristics of untested animals, prediction of future characteristics and identification of exceptional animals.  相似文献   
107.
The effect of bensulfuron-methyl (BSM) on a soil microbial community in a model paddy microcosm was studied. Total bacterial numbers in the overlying water and surface soil were monitored for 2 months after the application of BSM at the field rate and a ten-fold field rate. Pentachlorophenol (PCP) was used for comparison. Neither chemical affected the total bacterial numbers remarkably, either in the overlying water or in the surface soil. In contrast, the nitrification potential was significantly suppressed by the BSM application. The bacterial community structure, as evaluated by the denaturing gradient gel electrophoresis (DGGE) of PCR amplification products from bacterial 16S rDNA, was unaffected by the BSM treatments over 8 weeks in the surface soil, compared with the control (no pesticide). In contrast, the surface soil exposed to PCP at a ten-fold field rate showed different patterns from the controls at 4 weeks and 8 weeks after application. The DGGE patterns of the overlying water were much more variable than those of the surface soil in any treatments. Cluster analysis showed that the BSM plots were classified within the same group as the control at 1 week after application and that the BSM and PCP plots from 2 weeks onward after application were grouped differently from the control. Of 22 clones excised from the DGGE gels, 20 clones belonged to the Proteobacteria and two belonged to the Verrucomicrobia. It was considered that the impact of BSM on the overall microbial community (total numbers, community structure of soil) was negligible, although BSM had an impact on some specific functions of the soil microbial community (nitrification) and a part of the community (overlying water).  相似文献   
108.
In order to understand the efficiency of residue-N use and to estimate the minimum input required to obtain a reasonable level of crop response, it is important to quantify the fate of the applied organic-N. The recovery of N from 15N-labelled Crotalaria juncea was followed in the soil and the succeeding maize crop. Apparent N recovery (ANR) by maize from unlabelled Crotalaria juncea, Crotalaria retusa, Calopogonium mucunoides, Mucuna pruriens and mineral fertilizer at three locations were also evaluated. The maize crop recovered 4.7% and 7.3% of the 15N-labelled C. juncea-N at 42 days after sowing (DAS) and at final harvest, respectively. The corresponding 15N recovery from the soil was 92.4% and 58.5%. The highest mean ANR of 57.4% was with mineral fertilizer, whereas the mean ANR of 14.3% from C. retusa was the lowest. A large pool substitution and added-N interaction effect was observed when comparing N recovery from the labelled and unlabelled C. juncea. The amount of residue-N accounted for by the isotope dilution method at 42 DAS was 97.1% and at final harvest 65.8%. The large residue-N recovery in the soil organic-N pool explains the residual effect usually observed with organic residue application.  相似文献   
109.
Carbon and nitrogen mineralisation of leather meal fertilisers were studied in two soils characterised by different respiration activity. Both C and N mineralisation were highest in the most active soil, and when leather meal was added as a powder rather than as 2- to 4- and 4- to 6-mm particles. Fast and slow soluble N pools were determined after extraction with cold water and with hot buffer, respectively. The N remaining after the second extraction with hot buffer was named slow-release N. The percentage of slow-release N rose as the size of the applied leather meal particles increased, whereas fast soluble N was highest in the coarsest (4-6 mm) fertiliser.  相似文献   
110.
Pesticides applied in extended agricultural fields may be controlled by means of bioindicators, such as honeybees, in which are the pesticides bioaccumulate. Liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC-APCI-MS) experiments with positive (PI) and negative (NI) ion modes were optimized for the analysis of 22 organophosphorus pesticides in honeybee samples. The extraction required 3 g of sample, which was extracted with acetone. The extract was purified with coagulating solution and reextracted with Cl(2)CH(2). Pesticides studied could be detected by both ionization modes except for parathion, parathion-methyl, and bromophos, which did not give signals in PI mode, and triazophos, which was not detected in NI mode. Fragmentation voltage and vaporizer temperature were optimized to achieve the highest sensitivity. The spectra profile of each pesticide in PI mode showed the [M + H](+) ion as the main signal, whereas in NI mode only fragment ions were shown. The detection limit obtained in selected ion monitoring mode ranged from 1 to 15 microg kg(-1). The average recoveries from spiked honeybees at various concentration levels (0.5-5 mg kg(-1)) exceeded 65% with relative standard deviations of 4-15%. The method was applied to real samples, in which residues of coumaphos and dimethoate were detected.  相似文献   
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