Anti-diabetic potential of ursolic acid stearoyl glucoside: a new triterpenic gycosidic ester from Lantana camara     

Kazmi I  Rahman M  Afzal M  Gupta G  Saleem S  Afzal O  Shaharyar MA  Nautiyal U  Ahmed S  Anwar F 《Fitoterapia》2012,83(1):142-146

A new stearoyl glucoside of ursolic acid, urs-12-en-3β-ol-28-oic acid 3β-d-glucopyranosyl-4′-octadecanoate and other compounds were isolated from the leaves of Lantana camara L. The structure of this new glycoside was elucidated and established by standard spectroscopic methods. In streptozotocin-induced diabetic rats it showed significant reduction in blood glucose level.  相似文献   

Corrigendum to “Anti-diabetic potential of ursolic acid stearoyl glucoside: A new triterpenic gycosidic ester from Lantana camara” [Fitoterapia 83 (2012) 142–146]     

Imran Kazmi  Mahfoozur Rahman  Muhammad Afzal  Gaurav Gupta  Shakir Saleem  Obaid Afzal  Md. Adil Shaharyar  Ujjwal Nautiyal  Sayeed Ahmed  Firoz Anwar 《Fitoterapia》2012

  相似文献   

Canine corneal fibroblast and myofibroblast transduction with AAV5     

Bosiack AP  Giuliano EA  Gupta R  Mohan RR 《Veterinary ophthalmology》2012,15(5):291-298

Objective The aims of this study were (1) to determine the efficacy of adeno‐associated vector serotype 5 (AAV5) for delivering gene therapy to canine corneal fibroblasts (CCFs) and myofibroblasts (CCMs) using enhanced green fluorescent protein (GFP) marker gene and (2) to evaluate the cytotoxicity of AAV5 to CCFs and CCMs using an in vitro model. Methods Healthy donor canine corneas were used to generate primary CCFs by growing cultures in minimal essential medium supplemented with 10% fetal bovine serum. Canine corneal myofibroblasts were produced by growing cultures in serum‐free medium containing transforming growth factor β1 (1 ng/mL). An AAV5 titer (6.5 × 10¹² μg/mL) expressing GFP under control of hybrid cytomegalovirus + chicken β‐actin promoters (AAV5‐gfp) was used to transduce CCF and CCM cultures. Delivered gene expression in CCFs and CCMs was quantified using immunocytochemistry, fluorescent microscopy, and real‐time PCR. Transduction efficacy of the AAV5 vector was determined by counting DAPI‐stained nuclei and EGFP‐positive cells in culture. Phase‐contrast microscopy, trypan blue, and dUTP nick end labeling (TUNEL) assays were used to determine the toxicity and safety of AAV5 in this canine corneal model. Results Topical AAV5 application successfully transduced a significant population of CCFs (42.8%; P < 0.01) and CCMs (28%; P < 0.01). Tested AAV5 did not affect CCF or CCM phenotype or cellular viability and did not cause significant cell death. Conclusions The tested AAV5 is an effective and safe vector for canine corneal gene therapy in this in vitro model. In vivo studies are warranted.  相似文献   

Landscaping and performance of some aesthetic plant species in hot, arid conditions of India     

Rajesh Kumar Gupta  Pradeep Chaudhry  Rameshwar Lal Srivastava 《林业研究》2012,23(2):305-309

Establishment of ornamental and aesthetic plants in hot, arid conditions of India is difficult due to the prevailing climatic, edaphic and biotic factors. Effect of turfgrass on the growth of ornamental plants in hot arid conditions has not been studied so far anywhere in the world. A study was conducted on the campus of Arid Forest Research Institute, Jodhpur, India to assess the performance of a few ornamental plant species in combination with turfgrass and without turfgrass with respect to different soil tilling intervals. Growth of plants was better with turfgrass than without turfgrass. We suggest adopting a soil tilling interval of 30 days to achieve optimum growth of ornamental plant species in terms of height and crown diameter. Our results can help reduce labor costs and achieving better landscapes in fewer days in hot urban conditions of Indian sub continent.  相似文献   

Development and utilization of novel SSRs in foxtail millet [Setaria italica (L.) P. Beauv.]     

Sarika Gupta  Kajal Kumari  Mehanathan Muthamilarasan  Alagesan Subramanian  Manoj Prasad 《Plant Breeding》2013,132(4):367-374

Although the foxtail millet [Setaria italica (L.) P. Beauv.] is recently regarded as a model crop for studying functional genomics of biofuel grasses, its genetic improvement to some extent was limited due to the non‐availability of molecular markers, particularly the microsatellite markers and the saturated genetic linkage map. Considering this, we attempted to generate a significant number of microsatellite markers in cultivar ‘Prasad’. Two hundred and fifty‐six clones were sequenced to generate 41.82‐kb high‐quality sequences retrieved from genomic library enriched with dinucleotide repeat motifs. Microsatellites were identified in 194 (76%) of the 256 positive clones, and 64 primer pairs (pp) were successfully designed from 95 (49%) unique SSR‐containing clones. The 67.4% primer designing ability, 100% PCR amplification efficiency and 45.3% polymorphic potential in the parents of F₂ mapping population established the efficacy of genomic microsatellites. All the 64 microsatellite markers displayed high level of cross‐species amplification (~67%) in 10 millets and non‐millets species. These experimental findings suggest the utility and efficacy of SSRs in diverse genotyping applications, resolving QTLs, phylogenetic relationships and transferability in several important grass species.  相似文献   

Simultaneous immunization of cattle with strain 19 Brucella abortus and infectious bovine rhinotracheitis (IBR) vaccines     

Barbara F. Smith B.SC. B.V.SC  L.B. Fastier M.SC. PH.D.  N.F. Hansen 《New Zealand veterinary journal》2013,61(1)

  相似文献   

Vaccination of sheep     

H.D. Seddon B.V.SC. 《New Zealand veterinary journal》2013,61(1)

  相似文献   

Letters to the editor     

J.M.B. Smith M.SC. PH.D. 《New Zealand veterinary journal》2013,61(5):83-84

Extract

In order to utilize spring growth efficiently under a system of grassland farming, a New Zealand dairy farmer endeavours to calve as many cows as possible within a four to six weeks' period. To maintain this calving pattern, herd members must conceive by 70 to 80 days after calving. Consequently normal postparturient uterine involution, together with a minimum of delay in the onset of ovarian activity and oestral cyclicity, is of vital importance. A study of these phenomena may be very rewarding in helping to achieve optimal reproductive efficiency.  相似文献   

Letters to the editor     

John M.B. Smith M.SC. PH.D. 《New Zealand veterinary journal》2013,61(10):210-211

Extract

Sir,—A 7-month-old lamb was presented for slaughter, one of a line of 460. The animal was in good condition and, except for a moderately enlarged abdomen, no abnormalities could be detected on ante-mortem examination.  相似文献   

What is your diagnosis? Abdominal fluid from a dog     

Aradhana Gupta  Jocelyn Garber  Natalie Fowlkes  Nathalie Rademacher  Keijiro Shiomitsu  Dawn E. Evans  Stephen D. Gaunt 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2013,42(1):113-114

  相似文献