Characterization of ovine strains of Mycobacterium paratuberculosis by restriction endonuclease analysis and DNA hybridization.     

G W De Lisle  D M Collins  H F Huchzermeyer 《The Onderstepoort journal of veterinary research》1992,59(2):163-165

Restriction endonuclease analysis and DNA hybridization revealed five ovine strains of Mycobacterium paratuberculosis from South Africa had identical DNA patterns to an ovine strain from Canada. Genetically this strain type has features in common with the two major groups of M. paratuberculosis.  相似文献   

Kinetics method for the quantitation of anthocyanidins, flavonols, and flavones in foods.     

H M Merken  C D Merken  G R Beecher 《Journal of agricultural and food chemistry》2001,49(6):2727-2732

Flavonoids are important dietary constituents owing to their health-promoting properties. As a result, simplified analytic techniques are required for the population of databases with food values so that associations between dietary intake and disease risk/incidence can be established. The current research provides a simplified sample preparation procedure for the accurate estimation of food anthocyanidins, flavones, and flavonols as aglycons. Traditionally, flavonoid aglycons have been formed by acidic hydrolysis. However, some flavonoid aglycons are slowly degraded by acid. A procedure has been developed whereby anthocyanidins and flavonols are deglycosylated with HCl in 50% aqueous methanol and the resulting aglycons subsequently quantified by application of pseudo-first-order kinetics to their degradation. Flavones are also deglycosolated under similar conditions but, at appropriate temperatures, their aglycons are stable in acid, so kinetics were not required for the quantitation of this subclass of flavonoids. Catechins and flavanones were rapidly degraded under the hydrolytic conditions used in these studies.  相似文献   

沙棘在西伯利亚面临的问题与解决办法   总被引：2，自引：1，他引：1  

P·L·贡恰诺夫  刘振和 《沙棘》2001,14(1):41-43

针对西伯利亚沙棘繁育发展中的问题，尤其是20世纪30年代从郭尔诺－阿尔泰地区引入的沙棘客体连续出现的品种变化，混杂和应淘汰等问题，指出：这里的沙棘，不但从野生发育形态方面出现诱变，而且目前在西伯利亚仅有20个左右繁育着的品种，在展示着原品种较为完备的特性；能按时结果；树冠低矮；有刺；浆果稳定。总的看，几乎在整个俄罗斯20个区域内传播着，占俄罗斯栽植品种的1／3。当然，每个品种都有有一个生物经济的所有制和栽植前的宣传发布与公认问题，解决这些问题，就能在各种不同地区加强推广和普及。但到目前为止，西伯利亚栽植繁育着的沙棘，还只是不多的原有一些品种，使用的还是过去认为效率高的多样化方法。  相似文献   

The Phytotoxicity of Various Graminicides in Durum Wheat in Greece     

D. Bilalis  P. Efthimiadis  & G. Karagiannis 《Journal of Agronomy and Crop Science》2001,187(2):121-126

The effect of three herbicides, diclofop-methyl (DM), imazamethabenz-methyl (IM) and fenoxaprop-ethyl (FE), was investigated at the recommended rate (×) and double the recommended rate (2×) in a durum wheat crop. FE had the smallest effect on root system characteristics, while IM had the greatest effect. For all herbicides, the double rate treatment gave a greater statistical reduction than the single rate for root system characteristics. FE had the smallest effect on the activity of aerobic microorganisms, while DM gave the greatest reduction, 10 days after application, and all three herbicides reduced earthworm abundance. DM gave the smallest reduction in the number of tillers and in plant height, while the double rate of IM gave the greatest reduction. For all three herbicides, double the recommended rate resulted in a significant reduction in the weight of 1000 seeds, while no statistically significant deviations were noted at the recommended rate. Finally, the use of all three herbicides resulted in significantly lower statistical yields compared to the sample (control plot), although there were no significant statistical differences among them, for either recommended or double recommended rates.  相似文献   

Purification of carbonic anhydrase isozyme VI (CA-VI) from swine saliva.     

T Nishita  M Sakomoto  T Ikeda  H Amasaki  M Shino 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(10):1147-1149

Salivary or secreted carbonic anhydrase (CA), which constitutes a new class of CA, designated CA-VI, was isolated. Swine CA-VI purified from swine saliva by inhibitor-affinity chromatography and ion exchange chromatography had a specific activity of 5,468 units/mg. The molecular weight was 250,000, as determined by gel filtration under non-denaturing conditions, and the subunit molecular weight was found to be 37,000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis, indicating that swine CA-VI consists of 7 subunits. The treatment of the enzyme with endo-N-acetylglucosaminidase F reduced its subunit molecular weight from 37,000 to 35,000 and 32,000. We raised a rabbit antibody against purfied swine CA-VI. Double immunodiffusion showed that anti-swine CA-VI serum reacted with swine CA-VI and swine saliva, but not with hemolysate (containing CA-I and CA-Il) or muscle extracts (containing CA-III). The concentration of CA-VI in swine saliva, measured using single radial immunodiffusion, was 0.027 +/- 0.017 mg/mg total protein.  相似文献   

Efficacy evaluation of plant protection products     

A. M. P. Lavadinho 《EPPO Bulletin》2001,31(3):345-352

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Pathotypes of Synchytrium endobioticum in Germany     

E. LANGERFELD  H. STACHEWICZ  J. RINTELEN† 《EPPO Bulletin》1994,24(4):799-804

Results are given of a survey on the occurrence of pathotypes (races) of Synchytrium endobioticum (potato wart) in both parts of re-unified Germany. An assortment of six differential cultivars appears to be sufficient to distinguish between the seven presently important German wart pathotypes. A total of ten pathotypes was recorded. Two West and two East German pathotypes seem to be identical, based on the results obtained with presently available cultivars. In contrast to pathotype 1 (common race) which prior to 1945 was distributed over the whole German territory, the 'new' pathotypes seem to prefer the central and south German mountain areas.  相似文献   

Effects of photostimulation at 18, 24 and 30 weeks of age on the productivity of female turkeys fed ad libitum or restricted until point of lay.     

P M Hocking 《British poultry science》1992,33(2):253-269

1. Turkey females were fed ad libitum or restricted from 6 weeks of age to achieve mean body weights of 0.6 of ad libitum fed birds on photostimulation at 18, 24 or 30 weeks of age. Body weight, food intake, egg production and hatchability were recorded to 54 weeks of age. 2. Restricted turkeys were fed ad libitum after first egg or 36 weeks of age. Food intake after 30 weeks of age averaged 170 g/bird/d and was similar for all treatments. At 54 weeks of age, restricted turkeys photostimulated at 24 and 30 weeks were not as heavy as turkeys photostimulated at 18 weeks and ad libitum fed turkeys. 3. A large proportion of restricted turkeys photostimulated at 18 weeks of age did not commence lay until 30 to 40 weeks and a significant number of restricted birds photostimulated at 24 weeks had short laying cycles. Restricted birds photostimulated at 30 weeks came into lay and showed similar persistency of lay to ad libitum fed turkeys. 4. Ad libitum fed turkeys laid 115.0, 122.0 and 101.0 and restricted turkeys 92.4, 99.5 and 103.4 eggs when photostimulated at 18, 24 and 30 weeks, respectively. The number of non-settable eggs was lower in restricted compared with ad libitum fed turkeys and declined with age at photostimulation. 5. Egg size at the end of the experiment was similar for ad libitum fed turkeys and restricted birds photostimulated at 18 weeks but was 2.3 g lower for restricted birds photostimulated at 24 and 30 weeks of age. 6. Hatchability was higher, and the proportion of second quality poults was lower with eggs from restricted turkeys. The mean numbers of poults hatched were 59.9, 75.1 and 66.0 for ad libitum fed and 71.7, 65.7 and 79.4 for restricted turkeys photostimulated at 18, 24 and 30 weeks of age.  相似文献   

Anesthesia for gastric dilatation/volvulus.     

P L Wong 《Veterinary Clinics of North America: Small Animal Practice》1992,22(2):471-474

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Zakosek,H. & F. Lenz (Hrsg.): Nitrat in Boden und Pflanze — unter besonderer Berücksichtigung des Gemüsebaus. Ulmer,Stuttgart 1993; 216 S., 49 Abb.; DM 68,-. ISBN 3-8001-5282-7     

P. Felix-Henningsen 《植物养料与土壤学杂志》1994,157(2):153-153

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