Study on animal intracorneal implantation model with xenogeneic swine corneal stroma     

WU Jing  HAO Nian  LI Shu-yan  XU Jin-tang  WANG Yan-ping 《园艺学报》2005,21(6):1193-1197

AIM: To study the immunogenicity and biocompatibility of xenogeneic swine corneal stroma as biological carrier for cornea reconstruction and to reconstruct corneal endothelial tissue with this carrier in vitro. METHODS: (1) The lymphocytes from the peripheral blood of F344 rats were immunologically labeled by anti-rat CD25-FITC and anti-rat CD4/CD8-PE, then determined by flow cytometry (FCM) at 12th， 90th day after intracorneal implantation with fresh and dehydrated swine corneal stroma. (2) The fresh and dehydrated grafts made of swine corneal stroma were implanted intralamellarly in corneas of New Zealand rabbits. Clinical examinations were performed monthly and histological examinations were made at 14th， 30th， 60th， 120th and 240th day. (3) The cat corneal endothelial cells were seeded on the Descemet's membrane of the dehydrated swine corneal stroma, then cultured in the medium with epidermal growth factor and laminin for 7 days. The morphology of reconstructed tissue was tested by microscope. RESULTS: (1) Compared to isograft group and negative control, the expression CD4＋CD25＋， CD8＋CD25＋ and CD4＋/CD8＋ of xenograft rat group implantation with swine corneal stroma did not appear significantly different in statistics (P>0.05). (2) In the total 12 rabbits, all the cornea grafts survived without rejection reaction， corneal haze or corneal neovascularization. The fresh grafts got transparent after 2 months, and the dehydrated grafts got transparent after 6 months. Histological study demonstrated both fresh and dehydrated stroma grafts had fused with rabbits'corneal stroma very well without lymphocytes infiltrating. (3) As shown in histological observations, the reconstructed cat corneal endothelial tissue was similar to the nature tissue. Cultured cat endothelial cells connected tightly to each other and attached to the Descemet's membrane closely. CONCLUSION: Swine corneal stroma has low immunogenicity and satisfying biocompatibility,it is an ideal biological carrier for cornea reconstruction in vitro.  相似文献   

搞好森林病虫害监测预报,促进林业生态建设和可持续发展     

郝宗信  龙炜 《江西植保》2005,28(1):28-29,27

就森林病虫害监测预报工作在林业和生态建设中的意义和作用，针对我省测报网络建设的现状及存在的问题，提出了江西测报体系建设的思路。  相似文献   

Effects of antibiotic treatment on the inflammatory mediator expression in peritoneum and the peritoneal transport function in the rat with acute bacterial peritonitis     

JIA Zhan-jun  YU Xue-qing  WANG Xin  LI Xiao-yan  CHEN Wen-fang  PENG Wen-xing  DOU Xian-rui  HAO Wen-ke  SUN Liao  ZHENG Zhi-hua  YIN Pei-da 《园艺学报》2005,21(7):1298-1304

AIM: To observe the effect of antibiotic treatment on the inflammatory mediator expression in peritoneum and the peritoneal transport function in rats with acute peritonitis, and explore its mechanisms. METHODS: Eighty-six SD rats were randomly divided into three groups. Control group (n=28) were treated with PBS (ip), peritonitis group (n=28) and treatment group (n=28) were challenged with the E.coli (ip), but at 3 h and 9 h gentamicin was given (ip) in treatment group. Seven rats of every group were randomly sacrificed at 24 h, 48 h, 72 h and 7 d. Peritoneal equilibration test (PET) was did before they were killed. Leukocyte count, pathological changes and the expression of CD45, NF-κB， IL-1β， TNF-α in peritoneum were examined. RESULTS: (1)The blood leukocytes in peritonitis group decreased strikingly, but did not change obviously in other two groups. The peritoneal fluid leukocytes in peritonitis group increased significantly from 24 h to 72 h, while in treatment group, it enhanced more strikingly than peritonitis group at 24 h, and recovered earlier. (2) Both in peritonitis group and treatment group, the expression of activated NF-κB, IL-1β, TNF-α and CD45 increased significantly, but the treatment group was lower than model group at 48 h and 72 h. The mRNA level of IL-1β and TNF-α had the same trend as their protein expression. (3) Compared with the control group, UF and D/D0 Glu decreased significantly in model group and treatment group, and D/PTP increased dramatically. The D/P TP in treatment group lowered obviously compared with peritonitis group, while the net UF and D/D0 Glu had not significant difference between treatment group and model group. CONCLUSION: Antibiotic treatment can partly decrease the expression of inflammatory mediators in peritoneum of rats with acute peritonitis and also can improve the protein transport ability to some extent, but can not improve the peritoneal ultrafiltration and the glucose transport function.  相似文献   

干旱沙区农田防护林营建模式与经营评价研究   总被引：5，自引：1，他引：5  

郝玉光  包耀贤  刘明虎  张景波 《干旱区资源与环境》2005,19(5):199-203

通过对干旱沙区人工绿洲农田防护林林木生长量的调查测定和样本树干解析数据,揭示了林木生长规律,建立了树干材积模型,为农田防护林合理更新提出了科学依据。利用相关依据指标,对现有农田防护林的营建模式进行了评价,提出了新的更新模式。  相似文献   

中国园艺学会第七届青年学术讨论会     

HAO Yu  XU Bo-wen  ZHENG Hong  HANG Xiao-tong  QIU Quan-ying  HUANG Qi-fu 《园艺学报》2005,32(6):1124-1127

中国园艺学会第九届第8次常务理事扩大会决定,“中国园艺学会第七届青年学术讨论会”由山东农业大学园艺科学与工程学院和山东省园艺学会承办,将于2006年7月或8月在山东泰安举行。会议交流主题:(1)园艺作物种质资源、遗传育种与生物技术;(2)园艺作物有机、无公害及标准化安全生  相似文献   

黑河(额济纳河)下游绿洲生态系统受损与生态保育对策的思考   总被引：12，自引：3，他引：12  

刘钟龄  朱宗元  郝敦元 《干旱区资源与环境》2001,15(3):1-8

额济纳绿洲是我国西北干旱区中心的一道生态防线。 2 0世纪后半世纪绿洲生态系统严重受损 ,景观结构急剧恶化 ,黑河的供水量逐年趋于下降 ,由 50年代的 1 2 .2 5亿 M3 下降到 90年代的 7.2 4亿 M3。地下水位也相应地降低 ,土地盐化也在加剧。胡扬与沙枣林面积由3 .45万公顷减少到 2 .2 0万公顷 ,并以老龄林为主。红柳灌丛也减少了 50 % ,并且多已成为稀疏矮化的群落。各种禾草草甸严重退化 ,多已演替成为苦豆子群落。为了维护我国西北的这一生态屏障 ,必须坚持以恢复与保护自然绿洲为主 ,恢复胡杨林、沙枣林及红柳灌丛的覆盖率 ;建立人工草地 ,改善土地覆盖 ,建设自然一人工复合绿洲。在绿洲建设与保育工程中必须保证充分供水 ,调控水盐动态 ,发挥生物一景观多样性的系统耦合效应 ,实现绿洲生态系统健康  相似文献   

犬冠状病毒基因疫苗表达载体的构建及其免疫原性研究   总被引：1，自引：0，他引：1  

乔军  夏咸柱  杨松涛  郝峰  高玉伟  郑晓一  黄耕 《畜牧兽医学报》2006,37(4):412-416

以pVAX1为载体首次构建了犬冠状病毒病基因的3种真核表达质粒。首先将犬冠状病毒大熊猫株（CCVDXMV）纤突蛋白（S）、膜蛋白（M）和核蛋白（N）基因进行了克隆和序列测定。将测序后的质粒pTS、pTM和pTN分别双酶切，回收目的基因片段并将其定向克隆到真核表达质粒pVAX1中得到重组质粒pVAXS、pVAXM和pVAXN。将这3种真核表达质粒通过脂质体介导法转染MDCK细胞，通过RT—PCR法进行转录水平的检测，并用间接ELISA法检测目的蛋白的表达情况。结果在pVAXS、pVAXM、pVAXN转染MDCK细胞36h后就可检测到目的基因的转录；在转染72h后可检测到3种目的蛋白的表达。动物免疫试验表明，3种真核表达载体能有效地诱导机体产生细胞免疫与体液免疫应答，这为CCV基因疫苗的研究奠定了良好的基础。  相似文献   

牛基因组DNA两种提取方法的比较研究   总被引：3，自引：0，他引：3  

程金华  朱化彬  王栋  郝海生  杨波 《畜牧兽医学报》2006,37(9):874-877

研究哺乳动物基因组DNA的水煮抽提法，并将传统的酚仿抽提法和水煮法进行比较，水煮法即通过对细胞的煮沸和冷却，使细胞破裂、蛋白变性，从而获得用于PCR扩增的模板DNA的方法。通过电泳分析和PCR扩增检测了所提取基因组DNA的完整性、可行性。检测时采用3对引物对2种方法提取的DNA进行了PCR扩增，同时对冷冻保存的基因组DNA也进行了扩增检测。结果表明：水煮法提取基因组DNA是一种快速、简便、经济、高效的方法。  相似文献   

鲁西黄牛体细胞克隆保种技术的研究     

朱化彬  程金华  孙秀柱  王栋  戴蕴平  龚国春  万荣  郝海生 《畜牧兽医学报》2006,37(12):1287-1292

以体外培养的鲁西黄牛耳皮肤成纤维细胞作核供体，研究利用体细胞克隆技术保存我国地方黄牛优良品种的技术方法。通过组织块培养法建立的鲁西黄牛（1♂，4♀）成纤维细胞系，作为核移植供体细胞，利用屠宰场母牛卵巢卵母细胞经体外培养成熟后作为核受体进行核移植，试验结果表明：重构胚的融合率为62．5％（242／387），分裂率为63．6％（154／242），体外培养第7天囊胚发育率为42．9％（66／154）。体外培养第7天的囊胚的内细胞团细胞（ICM）与滋养层细胞数平均为37和47，ICM占44．2％。体外发育到7d的囊胚新鲜胚胎的移植妊娠率（新鲜胚胎）移植受体10头，60d妊娠率为20％（2／10）。  相似文献   

中药对畜禽免疫功能影响的研究进展     

郝福星  刘莉  吴金节 《动物医学进展》2006,27(Z1):62-65

文章综述了中药对畜禽免疫器官重量、单核-巨噬细胞系统功能、特异性免疫功能、细胞因子等的影响,阐述了中药调节畜禽免疫功能的机理.  相似文献