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Equine viral arteritis (EVA) caused by the equine arteritis virus, member of the genus arterivirus within the family of Togaviridae was recently isolated from the seminal plasma of two stallions indicating that the virus infection is also prevalent in the Federal Republic of Germany despite the apparent lack of acute clinical symptoms in the horse population. These findings are further supported by data from serological screenings. Out of 739 horse sera, 28 (3.8%) were found to have EVA virus-specific antibodies with titers greater than or equal to 4. It is important to note that the percentage of sero-positive horses was found to be increased from 1.8% in 1987-1988 to 6.8% in 1989. A voluntary prophylactic programme based on virological and serological tests is proposed to prevent further spread of the EVA virus infection. The pathogenesis, clinical symptoms and diagnosis of EVA are also reviewed. 相似文献
204.
Relaxin is a pregnancy-specific hormone in the queen and is produced by the placenta. Both serum and urinary relaxin levels can be used to diagnose and monitor pregnancy in the cat; however, only serum levels are commonly measured in practice. The present study aimed to assess whether urine could be used for the rapid diagnosis of pregnancy at an early stage in domestic cats using a bench-top kit to detect relaxin. Paired serum and urine samples were collected during the first month of gestation in six cats. The samples were tested by applying neat serum, urine or urine diluted in non-pregnant cat serum to the Witness Relaxin kit. Relaxin concentrations in the paired samples were also measured by radioimmunoassay. All undiluted urine samples from pregnant cats tested negative using the bench-top kit; however, the kit was able to detect relaxin in urine after dilution with non-pregnant cat serum. Using this as the test sample, the kit was accurate at diagnosing pregnancy from 28 days after mating and some samples tested positive at 21 days after mating. This preliminary work could lead to the development of a home pregnancy test for cats. 相似文献
205.
Haas B 《DTW. Deutsche tier?rztliche Wochenschrift》2001,108(12):508-513
Because foot-and-mouth disease has the potential for an explosive spread, instant and reliable diagnosis is of special importance. In this article the clinical examination, types and shipment of samples as well as the current methods of laboratory diagnosis by detection of FMD-virus, antigen, nucleic acid and antibodies are reviewed. Special emphasis is laid on the differentiation between vaccinated and infected animals, in respect to conventional as well as novel vaccines. 相似文献
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DDT: interaction with nerve membrane conductance changes 总被引:5,自引:0,他引:5
The falling phase of action potentials of lobster giant axons is prolonged by DDT; finally a plateau phase is produced like cardiac action potentials. In axons poisoned with DDT, peak transient (sodium) currents associated with step depolarizations are turned off very slowly, and steady-state (potassium) currents are markedly suppressed. These two changes would cause the prolongation of action potentials and are considered the major ionic mechanisms of DDT action. 相似文献
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Dynamics of pear-pathogenic Stemphylium vesicarium in necrotic plant residues in Dutch pear orchards
Jürgen Köhl Peter-Frans de Jong Pieter Kastelein Belia H. Groenenboom-de Haas Ron H. N. Anbergen Henny Balkhoven Jos P. Wubben 《European journal of plant pathology / European Foundation for Plant Pathology》2013,137(3):609-619
Brown spot disease on pear caused by Stemphylium vesicarium may affect leaves and fruits. Inoculum sources present on orchard floors play an important role in the epidemiology of pear brown spot. The pathogen can overwinter on plant residues and multiply and spread on the residues during the growing season. In the Netherlands, brown spot characteristically occurs only in a fraction of the orchards per season. Until now, no tools are available for Dutch pear growers to predict the risk of brown spot in specific orchards. As a consequence, preventive fungicide sprayings are common. The concentration of DNA of pear-pathogenic S. vesicarium was quantified by a specific TaqMan-PCR assay for various types of plant residues present on orchard floors to evaluate their importance as potential inoculum source. The pathogen was often found in residues of pear leaves, grasses and weeds, but only occasionally in mummies and prunings. Studies of the population dynamics showed that S. vesicarium decreased in dead pear leaves during early winter whereas pathogen populations developed with irregular pattern during the growing season on residues of weeds and grasses. Based on DNA concentrations of S. vesicarium in plant residue samples taken in 78 to 106 orchards in the springs of 2010, 2011 and 2012, the risk of brown spot development could be predicted for individual orchards. Such a risk prediction will allow growers to adapt their fungicide spray schedules to avoid unnecessary sprays in low-risk orchards. 相似文献