Sequencing of Culex quinquefasciatus establishes a platform for mosquito comparative genomics     

Arensburger P  Megy K  Waterhouse RM  Abrudan J  Amedeo P  Antelo B  Bartholomay L  Bidwell S  Caler E  Camara F  Campbell CL  Campbell KS  Casola C  Castro MT  Chandramouliswaran I  Chapman SB  Christley S  Costas J  Eisenstadt E  Feschotte C  Fraser-Liggett C  Guigo R  Haas B  Hammond M  Hansson BS  Hemingway J  Hill SR  Howarth C  Ignell R  Kennedy RC  Kodira CD  Lobo NF  Mao C  Mayhew G  Michel K  Mori A  Liu N  Naveira H  Nene V  Nguyen N  Pearson MD  Pritham EJ  Puiu D  Qi Y  Ranson H  Ribeiro JM  Roberston HM  Severson DW  Shumway M 《Science (New York, N.Y.)》2010,330(6000):86-88

Culex quinquefasciatus (the southern house mosquito) is an important mosquito vector of viruses such as West Nile virus and St. Louis encephalitis virus, as well as of nematodes that cause lymphatic filariasis. C. quinquefasciatus is one species within the Culex pipiens species complex and can be found throughout tropical and temperate climates of the world. The ability of C. quinquefasciatus to take blood meals from birds, livestock, and humans contributes to its ability to vector pathogens between species. Here, we describe the genomic sequence of C. quinquefasciatus: Its repertoire of 18,883 protein-coding genes is 22% larger than that of Aedes aegypti and 52% larger than that of Anopheles gambiae with multiple gene-family expansions, including olfactory and gustatory receptors, salivary gland genes, and genes associated with xenobiotic detoxification.  相似文献   

The Use and Exchange of Biological Control Agents for Food and Agriculture     

J.C.van Lenteren  ;M.J.W.Cock  ;J.Brodeur  ;B.Barratt  ;F.Bigler  ;K.Bolckmans  ;F.Haas  ;P.G.Mason  ;J.R.P.Parra 《中国生物防治》2010,(Z1):123-127

The report sets out to summarize the past and current situation regarding the practice of biologicalcontrol inrelationtothe use and exchange of genetic resources relevant for BCAs.It considers the twomain categories of biological control:classical and augmentative.Allowing access to BCAs for use inanother country imposes no risk of liability to the source country.Local scientific knowledge abouthabitats,fauna andflora,can be helpful  相似文献   

Analysis of the H gene, the central untranslated region and the proximal coding part of the F gene of wild-type and vaccine canine distemper viruses.   总被引：1，自引：0，他引：1  

L Haas  H Liermann  T C Harder  T Barrett  M L?chelt  V von Messling  W Baumg?rtner  I Greiser-Wilke 《Veterinary microbiology》1999,69(1-2):15-18

This paper summarizes the results of the genetic analysis of several parts of the genome of canine distemper virus (CDV) field isolates and vaccine viruses. The haemagglutinin (H) gene analysis showed that recent viruses did not differ significantly from vaccine strains. The analysis of the long untranslated region between the matrix (M) and fusion (F) gene revealed distinct genetic heterogeneity. The putative F protein start codon AUG461 of vaccine strain Onderstepoort was found to be mutated in all wild-type isolates and in another vaccine strain. The proximal coding part of the F gene was well conserved. Phylogenetic analysis of this segment showed the presence of several cocirculating CDV genotypes.  相似文献   

Biotin-streptavidin enzyme-linked immunosorbent assay for the detection of antibodies to Campylobacter jejuni and C. coli in chickens.     

B Haas  K H Hinz  G Glünder 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1999,46(3):163-171

An enzyme-linked immunosorbent assay (ELISA) was developed in a homologous system with bacterial ultrasonic-treated proteins as the antigen and antisera from chickens infected orally and subcutaneously with the strain Campylobacter jejuni serovar 6 (CJ 6). The cut-off level was determined using antisera from non-infected specific-pathogen-free chickens up to the age of 10 weeks. The suitability of the ELISA system was verified using antisera taken from chickens orally infected at the age of 4 weeks with CJ 1, 6, 28 or 36 or with Campylobacter coli serovar 28 (CC 28). The development of antibodies was monitored up to 6 weeks post-infection (p.i.). Sera from chickens infected with CJ 1, 6, 36 or CC 28 contained specific antibodies to Campylobacter, whereas in those infected with CJ 28 no specific antibodies were found. Distinct cross-reactions were observed between CJ 6, 28 and CC 28 antigens and their antisera 6 weeks p.i., while poor cross-reactions were found with antisera to CJ 1 and 28. Antibodies to strains of all heterologous serovars were successfully detected with an antigen pool comprised of CJ 1, 6 and 36 antigens. In 11 out of the 12 field sera obtained from 5- and 9-week-old broiler chickens suffering from campylobacteriosis, high specific antibody titres to Campylobacter jejuni were found.  相似文献   

Working with veterinary students.     

K B Haas 《Journal of the American Veterinary Medical Association》1992,200(2):170-171

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Probe seeks gene: nucleic acid hybridization as a current contribution to virologic diagnosis     

L Haas  O R Kaaden 《DTW. Deutsche tier?rztliche Wochenschrift》1989,96(2):66-69

Molecular nucleic acid hybridization is based on the ability of single-stranded DNA/RNA to form hybrids with complementary labeled nucleic acids. This review shortly describes the components of this technique and presents the most important hybridization methods (spot-, Southern blot-, in situ-hybridization). The (potential) applications of nucleic acid hybridization as a diagnostic tool are discussed (e.g., for viruses which grow insufficiently or not at all in cell culture; virus latency; viruses with labile or antigenically variable envelope proteins, resp.; for virus classification) and yet existing limitations are indicated. An impetus for this technique in means of diagnostic application is expected to result from the Polymerase Chain Reaction (PCR) in the next future.  相似文献   

Pyrrolizidine alkaloid poisoning of yaks: diagnosis of pyrrolizidine alkaloid exposure by the demonstration of sulphur-conjugated pyrrolic metabolites of the alkaloid in circulating haemoglobin     

H WINTER  AA SEAWRIGHT  J HRDLICKA  AR MATTOCKS  R JUKES  KINZANG WANGDI  KB GURUNG 《Australian veterinary journal》1993,70(8):312-313

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Biological Control of Botrytis cinerea in Cyclamen with Ulocladium atrum and Gliocladium roseum Under Commercial Growing Conditions     

Köhl J  Gerlagh M  De Haas BH  Krijger MC 《Phytopathology》1998,88(6):568-575

ABSTRACT The effect of treatments with conidial suspensions of Ulocladium atrum and Gliocladium roseum on leaf rot of cyclamen caused by Botrytis cinerea was investigated under commercial greenhouse conditions. Spraying U. atrum (1 x 10(6) conidia per ml) or G. roseum (2 x 10(6) conidia per ml and 1 x 10(7) conidia per ml) at intervals of 2 to 3 weeks during the production period and spraying U. atrum (1 x 10(6) conidia per ml) at intervals of 4 to 6 weeks resulted in a significant reduction of natural infections of petioles by B. cinerea. U. atrum or G. roseum (1 x 10(7)conidia per ml) was as effective as the standard fungicide program. B. cinerea colonized senesced leaves within the plant canopy and infected adjacent petioles and leaves later. The antagonists colonized senesced leaves and reduced B. cinerea development on these leaves. Thus, the inoculum potential on petioles adjacent to necrotic leaf tissues was reduced. The fate of U. atrum conidia on surfaces of green cyclamen leaves during a 70-day period after application was studied. The number of conidia per square centimeter of leaf surface remained relatively constant during the entire experiment. Sixty percent of the conidia sampled during the experiments retained the ability to germinate. When green leaves were removed from the plants to induce senescence and subsequently were incubated in a moist chamber, U. atrum colonized the dead leaves. Senesced leaves also were colonized by other naturally occurring fungi including B. cinerea. On leaves treated with U. atrum from all sampling dates, sporulation of B. cinerea was significantly less as compared with the untreated control. Our results indicate that early applications of U. atrum before canopy closure may be sufficient to achieve commercially satisfactory control of Botrytis leaf rot in cyclamen.  相似文献   

Detection of phocine distemper virus using the polymerase chain reaction     

L Haas  T Barrett  T Harder  C J Bostock 《DTW. Deutsche tier?rztliche Wochenschrift》1990,97(2):93-95

During the fatal seal epizootics in the North and Baltic Seas in summer 1988 a virus was isolated which was shown to be the causal agent. It was subsequently classified as morbillivirus by neutralization assays, reaction with monoclonal antibodies and nucleic acid hybridization studies. The virus (tentatively called Phocine Distemper Virus, PDV) is difficult to grow in culture making rapid diagnosis difficult. We have used the Polymerase Chain Reaction (PCR) as an alternative and fast method to detect the presence of virus-specific nucleic acid and we describe here the amplification of cell culture derived PDV RNA in a "one-tube" reaction using heterologous (Rinderpest Virus cDNA derived) F gene primers. The resulting 370 bp DNA fragment was shown to be morbillivirus derived by Southern blot hybridization using cloned RPV F gene as probe.  相似文献   

Methyl bromide and PCNB for sclerotium crown rot control in iris     

Hillela Vigodsky Haas 《Phytoparasitica》1976,4(3):201-205

Methyl bromide (MB) at rates of 500, 1000 and 1500 kg/ha, Terraclor 75 WP (PCNB) at 150 and 300 kg/ha, and combinations of the two, were studied for control ofSclerotium rolfsii prior to iris cultivation. Sclerotia buried 10 cm deep in soil were all killed by MB at 500 kg/ha; at greater depths higher doses were required. Bulbs harvested from PCNB- and PCNB + MB-treated plots were healthy; 75% of the bulbs in control plots were infected at harvest. When MB was used alone, the soil became re-infested (2–6% diseased plants). Bromide toxicity, correlated with the MB dose applied, appeared 41/2 months after planting; leaves yellowed and senesced prematurely. MB treatments also reduced bulb size, and residual phytotoxicity was found when the bulbs from treated plots were planted in the following year.  相似文献