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Pharmacokinetics, metabolism and urinary detection time of flunixin after intravenous administration in camels 总被引:4,自引:0,他引:4
I.A. Wasfi N.S. Boni A.A. Abdel Hadi M. ElGhazali O. Zorob N.A. Alkatheeri & I.M. Barezaiq 《Journal of veterinary pharmacology and therapeutics》1998,21(3):203-208
The pharmacokinetics of flunixin were determined after an intravenous dose of 1.1 mg/kg body weight in six camels and 2.2 mg/kg body weight in four camels. The data obtained (mean ± SEM) for the low and high dose, respectively, were as follows:
The elimination half-lives ( t½β ) were 3.76 ± 0.24 and 4.08 ± 0.49 h, the steady state volumes of distribution ( V dss ) were 320.61 ± 38.53 and 348.84 ± 35.36 mL/kg body weight, total body clearances ( Cl T ) were 88.96 ± 6.63 and 84.86 ± 4.95 mL/h/kg body weight and renal clearances ( Cl r ) were 0.52 ± 0.09 and 0.62 ± 0.18 mL/h/kg body weight. A hydroxylated metabolite of flunixin was identified by gas chromatography/mass spectrometry (GC/MS) under electron and chemical ionization and its major fragmentation pattern was verified by tandem mass spectrometry (GC/MS/MS) using neutral loss, daughter and parent scan modes. The detection times for flunixin and its hydroxylated metabolite in urine after an intravenous (i.v.) dose of 2.2 mg/kg body weight were 96 and 48 h, respectively. 相似文献
The elimination half-lives ( t
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The pharmacokinetics of orphenadrine (ORPH) following a single intravenous (i.v.) dose was investigated in six camels (Camelus dormedarius). Orphenadrine was extracted from the plasma using a simple sensitive liquid–liquid extraction method and determined by gas chromatography/mass spectrometry (GC/MS). Following i.v. administration plasma concentrations of ORPH decline bi-exponentially with distribution half-life (t1/2α) of 0.50 ± 0.07 h, elimination half-life (t1/2β) of 3.57 ± 0.55 h, area under the time concentration curve (AUC) of 1.03 ± 0.10 g/h l−1. The volume of distribution at steady state (Vdss) 1.92 ± 0.22 l kg−1, volume of the central compartment of the two compartment pharmacokinetic model (Vc) 0.87 ± 0.09 l kg−1, and total body clearance (ClT) of 0.60 ± 0.09 l/h kg−1. Three orphenadrine metabolites were identified in urine samples of camels. The first metabolite N-desmethyl-orphenadrine resulted from N-dealkylation of ORPH with molecular ion m/z 255. The second N,N-didesmethyl-orphenadrine, resulted from N-didesmethylation with molecular ion m/z 241. The third metabolite, hydroxyl-orphenadrine, resulted from the hydroxylation of ORPH with molecular ion m/z 285. ORPH and its metabolites in camel were extensively eliminated in conjugated form. ORPH remains detectable in camel urine for three days after i.v. administration of a single dose of 350 mg orphenadrine aspartate. 相似文献
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1. Changes in respiratory rate (f), rectal temperature (Tr ) and blood acid‐base values were measured in laying hens exposed to ambient temperatures (Ta) of 32, 35, 38 or 41 °G.
2. At Ta 32 °G there was no panting. At Ta 35 °G panting occurred without any increase in Tr but there was a slight alkalosis (pH 7.55).
3. At Ta 38 °G Tr increased and panting was accompanied by moderate alkalosis (pH 7.58).
4. At Ta 41 °G Tr increased considerably and severe alkalosis developed (pH 7.65).
5. From the relation between Tr , f and pH it is concluded that some degree of alkalosis is a normal response to panting in the laying hen. 相似文献
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Tariq M Ali G Hadi F Ahmad S Ali N Shah AA 《Pakistan journal of biological sciences: PJBS》2008,11(2):255-259
The objective of the present study was to develop an effective protocol for optimum callus induction and complete plant regeneration for four varieties of rice (Oryza sativa L.) i.e., Super Basmati, Basmati-370, Basmati-371 and Fakhre Malakand. Calli were induced from mature seed scutelum. The Murashige and Skoog (MS) and Chu's N6 media containing hormone 2, 4-D (2, 4-Dichlorophenoxy acetic acid) in different concentrations were used for callus induction. Fakhre Malakand produced maximum calli on N6 media containing 3 mg L(-1) 2,4-D. while other three varieties showed maximum callus induction on N6 media containing 2.5 mg L(-1) 2,4-D. N6 media was found better than MS media for callus induction. For complete plant regeneration the calli of two varieties i.e., Basmati-370 and Basmati-371 were plated on N6 media containing different concentrations of NAA (1-Naphthalene acetic acid) and BAP (6-benzyl aminopurine). The maximum regeneration frequency (%) was observed on N6 media containing NAA 1 mg L(-1) and BAP 2.5 mg L(-1). It took 27-30 days for the callus to regenerate into a complete plant. Basmati-370 produced 4-7 plantlets per callus whereas Basmati-371 produced 4-8 plantlets per callus with regeneration frequencies of 61 and 69%, respectively. 相似文献
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