Generation of mature monocyte-derived dendritic cells in the presence of heparin and monocyte conditioned medium: phenotypic and functional comparison     

Delirezh N  Majedi L  Asri Rezaei S  Ranjkeshzadeh H 《Iranian Biomedical Journal》2011,15(3):79-84

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ORF8 ∆382 Mutation: A Possible Viral Prognostic Biomarker for the Severity of the COVID-19     

Alireza Tabibzadeh  Maryam Esghaei  Farhad Zamani  Saber Soltani  Parastoo Yousefi  Mohammad Hadi Karbalaie Niya 《Iranian Biomedical Journal》2021,25(4):308

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Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity     

Sayyed Mohammad Hadi Alavi  Pavla Postlerová-Maňásková  Azadeh Hatef  Martin Pšenička  Jana Pěknicová  Kazuo Inaba  Andrzej Ciereszko  Otomar Linhart 《Fish physiology and biochemistry》2014,40(5):1393-1398

In mammals, proteases are present in sperm acrosome and play key role in fertilization. Sturgeon sperm has an acrosome, but its physiology, biochemistry, and potential role in fertilization are unknown. In the present study, we have observed high protease activity in acidic extract of intact sperm compared to that of seminal plasma in sterlet (Acipenser ruthenus). The protease activity was decreased and increased in acidic extract of motility-activated sperm and in the activation medium, respectively. Molecular analysis revealed total protease and serine (acrosin) protease activities in sperm acidic extract which was accumulated in a protein band with relative molecular mass of 35 kDa. Immunoelectron microscopy using an affinity-purified polyclonal antibody for boar acrosin localized the protease at the acrosome region. Moreover, initiation of sperm motility was inhibited after activation in the presence of inhibitors for both trypsin-like and chymotrypsin-like proteases, while the effects of protease inhibitors on sperm velocity were uncertain. Our results indicate similarities in physiology and biochemistry of acrosome between sturgeon and mammals and suggest potential role of protease in the initiation of sperm motility in sturgeon.  相似文献   

Fish meal replaced by hydrolysed soybean meal in diets increases growth and improves the antioxidant defense system of silver catfish (Rhamdia quelen)     

Juliano Uczay  Eduardo Kelm Battisti  Rafael Lazzari  Marcos Luiz Pessatti  Thamara Luisa Staudt Schneider  Luiza Beatriz Hermes  Nilce Coelho Peixoto  Thiago El Hadi Perez Fabregat 《Aquaculture Research》2019,50(5):1438-1447

One of the current challenges of global fish farming is developing plant‐based proteins as a feasible substitute for fishmeal in the formulation of aquafeeds. Substitution levels of fishmeal with hydrolyzed soybean meal (HSM) in diets for silver catfish (Rhamdia quelen) were evaluated for growth, protein metabolism and antioxidant activity. The experiment was carried out in a recirculation system of 20 tanks of 250 L. A total of 300 silver catfish juveniles were used to compose five treatments and four replicates per treatment. Levels of soybean hydrolysate (0%, 5%, 10%, 15% and 20%) were tested in diets (39% protein and 3,500 Kcal) for 60 days. Silver catfish growth was higher at all inclusion levels of HSM when compared to the control diet. No change was shown for body protein deposition. The production of nitrogenous compounds in the liver and muscle was also unaffected by any of the diets. The diet containing 20% HSM promoted an increase in the amounts of non‐protein thiols in the gills and brain. The HSM substitution levels of up to 20% of fishmeal in the diet increases growth and contributes to silver catfish antioxidant activity.  相似文献