Changes in running velocity at heart rate 200 beats/min (V200) in young thoroughbred horses undergoing conventional endurance training     

Ohmura H  Hiraga A  Matsui A  Aida H  Inoue Y  Sakamoto K  Tomita M  Asai Y 《Equine veterinary journal》2002,34(6):634-635

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Detection of the anti-P53 antibodies in dogs with tumors     

Kanaya N  Okuda M  Toyama N  Oikawa T  Inokuma H  Morimoto M  Hayashi T  Une S  Nakaichi M  Taura Y  Tsujimoto H  Onishi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):973-979

To detect the anti-P53 antibodies of dogs with tumors, a GST-recombinant canine (rc) P53 fusion protein was expressed and purified. Immunoblot analysis was performed using this GST-rcP53 fusion protein as an antigen and serum samples from dogs suffering from tumors as primary antibodies. Out of 16 serum samples obtained from various tumor cases, four samples showed reaction with GST-rcP53. In contrast, serum from other 12 dogs with tumors, four dogs with non-neoplastic diseases and two control healthy dogs (as controls) did not show any reaction with GST-rcP53 in immunoblotting. The p53 gene mutation and the P53 protein expression were examined, using the tumor tissues to explore the relationship between the existence of the GST-rcP53 bands, gene mutations of p53 and the accumulation of P53 protein. One case, which showed a clear GST-rcP53 band, had a point mutation of the p53 cDNA and showed nuclear accumulation of P53 protein. These results suggest that the anti-P53 antibodies are also produced in tumor dogs with p53 gene mutations.  相似文献   

Availability of NMR microscopic observation of mouse embryo disorder: examination in malformations induced by maternal administration of retinoic acid     

Sugimoto M  Manabe N  Morita M  Tanaka T  Okamoto R  Imanishi S  Miyamoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(5):427-433

Nuclear magnetic resonance (NMR) microscopy is a magnetic resonance imaging method with enhanced spatial resolution due to the use of a high static magnetic field and high magnetic field gradients. It is considered to be a useful tool for non-invasive and continuous investigation of tissue and organs at the histological level. In this study, we applied NMR microscopy to assessment of morphology in mouse embryos using a developmental disorder model induced by retinoic acid administration. Pregnant mice were given 50 mg/kg all-trans retinoic acid at 8.5 dpc. Embryos were collected at several time points after treatment and examined by NMR microscopy after fixation. Two-dimensional and three-dimensional spin echo sequences were used. Tissue contrast on two-dimensional images changed according to length of repetition time and echo time, and also to developmental stage of embryos. Two-dimensional and three-dimensional images nondestructively demonstrated defects in development of the skeleton and soft tissue, e.g. hypoplasia of vertebrae in the lumbar and tail regions and dysplasia of the spinal cord, in embryos exposed to retinoic acid. These morphological abnormalities were confirmed by conventional assessment after imaging. Although further improvements are required, NMR microscopy will provide a new approach for multi-parameter assessment of embryonic development under physiological and pathological conditions.  相似文献   

TRAIL-decoy receptor 1 plays inhibitory role in apoptosis of granulosa cells from pig ovarian follicles     

Wada S  Manabe N  Nakayama M  Inou N  Matsui T  Miyamoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(5):435-439

Previously, we histochemically examined the localization of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) and its receptors in porcine ovarian follicles, and demonstrated a marked reduction in the expression of TRAIL-decoy receptor-1 (DcRI) in granulosa cells of atretic follicles. In the present study, to confirm the inhibitory activity of DcR1 in granulosa cells, granulosa cells prepared from healthy follicles were treated with phosphatidylinositol-specific phospholipase C (PI-PLC) to cleave glycophospholipid anchor of DcR1 and to remove DcR1 from the cell surface, and then incubated with TRAIL. PI-PLC treatment increased the number of apoptotic cells induced by TRAIL. The present finding indicated the possibility that TRAIL and its receptors were involved in induction of apoptosis in granulosa cells during atresia, and that DcR1 plays an inhibitory role in granulosa cell apoptosis.  相似文献   

A retrospective study on adverse reactions to canine vaccines in Japan     

Ohmori K  Masuda K  Sakaguchi M  Kaburagi Y  Ohno K  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(9):851-853

Adverse reactions to vaccines were examined in 311 canine cases reported to the Ministry of Agriculture, Forestry and Fisheries in Japan during the period of 6 years from April of 1994 to March of 2000, and classified according to their clinical symptoms. There were 27 cases of adverse reactions to rabies virus vaccines. Gastrointestinal symptoms were the most frequently observed (26%), followed by respiratory and/or cardiovascular symptoms (22%) and dermatologic symptoms (11%). There were 284 cases of adverse reactions to non-rabies monovalent vaccines and mixed vaccines. Dermatologic symptoms were the most frequently observed (53%), followed by gastrointestinal symptoms (16%) and respiratory and/or cardiovascular symptoms (14%). Of the total 311 cases, 11 (3.5%) died of adverse reactions to vaccines.  相似文献   

Complex vertebral malformation in a stillborn Holstein calf in Japan     

Nagahata H  Oota H  Nitanai A  Oikawa S  Higuchi H  Nakade T  Kurosawa T  Morita M  Ogawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(12):1107-1112

A female stillborn Holstein calf with shortened cervical and thoracic regions, protrusion of the tongue, and bilateral symmetric flexural contraction of the anterior limbs was delivered on gestation day 281. Multiple hemivertebrae, fused and misshaped vertebrae, synostosis and scoliosis of cervical, thoracic and lumbar vertebral column were found in the affected calf by radiographic and computed tomographic (CT) analysis. Ten pairs of ribs were present and the sternum consisted of 9 sternebrae. Multiple morphologic abnormalities including fusion, malformation, and displacement, were found in the ribs and sternum. Cardiac anomalies, including atrial septal defect and hypertrophy of right ventricle, were observed. DNA-polymerase chain reaction (PCR) analysis demonstrated that amplified product from the liver DNA of the affected calf had identical pattern to that associated with complex vertebral malformation (CVM) of Holstein calves and that her dam was a heterozygous carrier of CVM. The affected calf was diagnosed as having CVM based on the DNA-PCR results and the characteristic findings, and was recorded as a first documentation of CVM confirmed in a Holstein calf in Japan.  相似文献   

Development of a system for measuring calcitonin in the stingray Dasyatis akajei (a cartilaginous fish): the possible involvement of stingray calcitonin in gonadal development     

Takashi Takagi  Toshio Sekiguchi  Yuichi Sasayama  Atsuhiko Hattori  Takushi X. Watanabe  Yoichiro Kitani  Yoshiaki Tabuchi  Hajime Matsubara  Ajai K. Srivastav  Tran Ngoc Hai  Thumronk Amornsakun  Nobuo Suzuki 《International Aquatic Research》2019,11(3):267-276

To elucidate the physiological role of calcitonin (CT) in stingrays (cartilaginous fish), an enzyme-linked immunosorbent assay (ELISA) system using a specific antibody against stingray CT has been developed. Synthetic stingray CT was subcutaneously injected into mice four times—once every 2 weeks—together with an adjuvant. We purified the IgG antibody fraction using the protein A affinity chromatography from collected antiserum. Evaluating the antibody titer, we found the antibody’s optimum dilution ratio to be 600 times. Competitive ELISA has been developed using the antibody diluted 600 times. Our antibody did not cross-react with teleost CTs and muscle extraction, but cross-reacted with stingray plasma and the extract of the ultimobranchial gland, the secretary organ of stingray CT. Using this ELISA, we measured the plasma CT level in stingrays and examined its correlation with several mineral concentrations. Plasma CT did not show significant correlation to calcium, magnesium, inorganic phosphorus, sodium, chlorine, or urea, although there was a correlation among the factors involved in osmoregulation, such as sodium, chlorine, and urea. On the other hand, plasma CT was significantly correlated to body weight and length. Furthermore, there was a significant correlation between plasma CT and gonad weight. Since plasma CT was correlated with the weight of liver, which is involved in the synthesis of egg yolk protein, we examined the influence of 17β-estradiol (E2) on CT secretion. After E2 injection, the plasma CT level increased significantly. This is the first study to demonstrate that E2 induced plasma CT secretion in cartilaginous fish.  相似文献   

Influence of Water Temperature on Morphological Deformities in Cultured Larvae of Japanese Eel,Anguilla japonica,at Completion of Yolk Resorption     

Tadahide Kurokawa  Takuma Okamoto  Koichiro Gen  Susumu Uji  Koji Murashita  Tatsuya Unuma  Kazuharu Nomura  Hajime Matsubara  Shin‐Kwon Kim  Hiromi Ohta  Hideki Tanaka 《Journal of the World Aquaculture Society》2008,39(6):726-735

The occurrence of morphological deformities under different rearing water temperatures (18, 20, 22, 24, 26, 28, and 30 C) was examined in Japanese eel larvae. The rates of hatching and survival until yolk resorption at 22–26 C were higher than those at other water temperatures. Fertilized eggs never hatched at 18 and 30 C. The rates of occurrence of abnormal larvae reared at the water temperatures 24–28 C were lower than those at 20 or 22 C. Pericardial edema and lower jaw deformities occurred most frequently at lower temperatures (20 and 22 C). In contrast, the incubation temperature did not significantly affect the relative frequency of some neurocranial deformities and of spinal curvature. These results imply that the optimal temperatures for rearing Japanese eel eggs and embryos are 24–26 C from the viewpoints of survival and deformity.  相似文献   

Isolation and identification of DPPH radical scavenging compounds in Kurosu (Japanese unpolished rice vinegar)   总被引：7，自引：0，他引：7  

Shimoji Y  Tamura Y  Nakamura Y  Nanda K  Nishidai S  Nishikawa Y  Ishihara N  Uenakai K  Ohigashi H 《Journal of agricultural and food chemistry》2002,50(22):6501-6503

Dihydroferulic acid (DFA) and dihydrosinapic acid (DSA) were isolated from Kurosu (unpolished rice vinegar) as the major constituents responsible for Kurosu's radical scavenging activity. The levels of antioxidative activity of DFA and DSA in DPPH radical scavenging were higher than those of their respective structurally related compounds, ferulic acid and sinapic acid. The concentrations of DFA and DSA were low in common rice vinegar (polished rice vinegar), suggesting that Kurosu is more advantageous than rice vinegars as an antioxidative food item. As the concentrations of DFA and DSA were low in unpolished rice, too, these acids are thought to be produced in Kurosu through the process of the fermentation from ferulic acid and sinapic acid, respectively.  相似文献   

Comparative study on yield and chemical composition of maize (Zea mays L.) and sorghum (Sorghum bicolor Moench) using different levels of manure application     

Ahmad Darobin LUBIS  Hajime KUMAGAI 《Animal Science Journal》2007,78(6):605-612

The effects of three levels of manure application (0, 4 and 8 ton/10 a) with basic fertilizer (N : P : K = 10:10:10 kg/10 a) on the yield and chemical composition of maize (Pioneer 32K61) and sorghum (FS501) forages were examined in 2 successive years, 2001–2002. Maize was harvested at yellow ripe stage, while sorghum at dough stage. Dry matter yield (DMY) of sorghum was significantly higher than that of maize in both 2001 and 2002. Although, DMY of both sorghum and maize were not significantly affected by manure levels in 2001, they increased with the level of manure in 2002. Maize had higher levels of crude protein (CP) and lower crude fiber concentration than those of sorghum in both 2001 and 2002. Manure application had no effect on chemical compositions of both maize and sorghum except for CP in both years and organic a fraction (Oa) concentration in 2002. The higher manure application might have greater effects on sorghum and maize production because of higher DMY in the second year without any increase in fiber fraction.  相似文献