The fluorescent antibody and indirect fluorescent antibody assays for diagnosing stunting syndrome of turkeys     

Reynolds DL  Oesper J  Ali A 《Avian diseases》2000,44(2):313-317

The fluorescent antibody (FA) assay was developed for detecting the stunting syndrome agent (SSA) from intestinal tissue. Similarly, the indirect fluorescent antibody (IFA) assay was developed for detecting serum antibodies to SSA. Convalescent antiserum from turkeys orally immunized with SSA was found to be the primary antibody of choice for the FA assay. Intestinal jejunal samples from poults inoculated 3-4 days postinoculation (DPI) was found to be the best antigen source for the IFA assay. SSA was detected from the intestinal tracts of experimentally inoculated birds at 2 DPI with highest level of reactivity at 3 DPI by the FA assay. After 4 DPI the level of SSA infectivity of the intestines waned to low levels. Serum antibody was detected from experimentally inoculated birds as early as 7 DPI with all birds tested seroconverting by 12 DPI. These assays should prove useful for future studies concerning stunting syndrome.  相似文献   

Construction of a microarray specific to the chicken immune system: profiling gene expression in B cells after lipopolysaccharide stimulation          下载免费PDF全文

Aimie J. Sarson  Leah R. Read  Hamid R. Haghighi  Melissa D. Lambourne  Jennifer T. Brisbin  Huaijun Zhou    Shayan Sharif 《Canadian journal of veterinary research》2007,71(2):108-118

The objective of this study was to profile gene expression in cells of the chicken immune system. A low-density immune-specific microarray was constructed that contained genes with known functions in the chicken immune system, in addition to chicken-expressed sequence tags (ESTs) homologous with mammalian immune system genes, which were systematically characterized by bioinformatic analyses. Genes and ESTs that met the annotation criteria were amplified and placed on a microarray. The microarray contained 84 immune system gene elements. As a means of calibration, the microarray was then used to examine gene expression in chicken B cells after lipopolysaccharide stimulation. Differential gene expression was observed at 6, 12, and 24 h but not at 48 h after stimulation. The results were validated by semiquantitative polymerase chain reaction. The microarray showed a high degree of reproducibility, as demonstrated by intra- and interassay correlation coefficients of 0.97 and 0.95, respectively. Thus, the low-density microarray developed in this study may be used as a tool for monitoring gene expression in the chicken immune system.  相似文献   

Effect of root applied 28-homobrassinolide on the performance of Lycopersicon esculentum     

B. Ali  S. HayatS. Aiman Hasan  A. Ahmad 《Scientia Horticulturae》2006

The roots of 20 days old seedlings of tomato (Lycopersicon esculentum Mill.) at the time of their transplantation, were dipped in 10⁻⁸, 10⁻⁷, 10⁻⁶ or 10⁻⁵ M of 28-homobrassinolide (HBR) for 15, 30 or 45 min and were allowed to grow in earthen pots, in a net house. The leaves of the plants, at days 30 and 60, possessed elevated quantities of nitrate reductase (NR), carbonic anhydrase (CA) and the contents of chlorophyll. The values for all the above characteristics were significantly higher than that of the water-fed control. The fruits borned at the treated plants were more in number and possessed a lower quantity of ascorbic acid than the control. Moreover, the fruits at ripening, had higher levels of lycopene and β-carotene. Among the treatments, 15 min feeding of 10⁻⁸ M HBR proved best.  相似文献   

Effects of Stocking Density and Monosex Culture of Freshwater Prawn Macrobrachium rosenbergii on Growth and Production in Concrete Tanks in Saudi Arabia     

Abdul Qayyum  Siddiqui  Yousef S.  Al-Hafedh  Ahmed H.  Al-Harbi Seikh A.  Ali 《Journal of the World Aquaculture Society》1997,28(1):106-112

Abstract This study was conducted to evaluate the effects of stocking density and monosex culture on growth, survival, yield and feed conversion ratio of freshwater prawn Macrobrachium rosenbergii in concrete tanks. Juvenile prawns with an average weight of 1.8 g were stocked into triplicate tanks at densities of 5, 10, 15 and 20 prawns/m², grown for 168 d and fed a 34% tilapia diet. Stocking density had significant effect on prawns. Final mean body weight decreased with the increasing density, being highest at 5 prawns/m² (29.6 9). and lowest at 20 prawns/m² (17.4 g). Total yield increased from 135 g/m² (1,350 kg/ha) at a density of 5 prawns/m² to 261 g/m² (2,610 kg/ha) at density 15/m^z to 245 g/m² (2,450 kg/ha) at 20/m^z. Feed conversion ratios were high and ranged from 3.7 (5 prawns/m²) to 5.6 (20 prawns/m²).
In monosex culture of freshwater prawns stocked in triplicate tanks at a density of 5 prawns/m² for 112 d, the all-male population had the best growth performance and feed conversion ratio, followed by the mixed-sex and all-female populations. The all-male population had 99% marketable prawns (>20 g) with an average yield of 159 g/m² (1,590 kg/ha); the mixed-sex population had 90% marketable prawns and the yield was 135 g/m^z (1,350 kg/ha); and the all-female population had 75% marketable prawns with an average yield of 108 g/m² (1,080 kgha).  相似文献   

The incidence of canine haematozoa in peninsular Malaysia     

C. Rajamanickam  E. Wiesenhutter  F.Md. Zin  J. Hamid 《Veterinary parasitology》1985,17(2):151-157

In 3 urban areas in Selangor, Peninsular Malaysia between 1973 and 1981, blood from 4080 dogs was examined for haematozoa. The following frequencies were found: Babesia gibsoni 17.7%; microfilariae of Dirofilaria immitis 9.6%; Hepatozoon canis 1.2%; B. canis 1.1%; Ehrlichia canis 0.2%; Trypanosoma evansi 0.1%. A detailed examination of B. gibsoni infections and microfilariasis due to D. immitis with regards to monthly distribution, breed frequency, sex and age, revealed that pedigree and non-pedigree dogs were equally susceptible to Babesia and microfilariae infections.  相似文献   

The economics of irrigation: Ian Carruthers and Colin Clark. Liverpool University Press, 1983. 300 pp., £10.50. ISBN 0-85323-464-7     

Ali Balaban 《Agricultural Water Management》1985,10(2):188-189

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The impact of grade of laryngeal function immediately prior to laryngoplasty and ipsilateral ventriculocordectomy on postoperative performance: 623 Thoroughbred racehorses (1998-2013)     

Ali H. Broyles  Rolf M. Embertson  J. Brett Woodie  Vinicius Machado 《Equine veterinary journal》2022,54(5):856-864

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Age estimation of Arabian mares by incisors morphometry and dentition changes     

Khazaeel  Kaveh  Pesarakli  Hamid  Mashhadi  Alireza Ghadrdan  Borujeni  Mahdi Pourmahdi 《Veterinary research communications》2022,46(2):405-417

Veterinary Research Communications - Accurate estimation of a horse's age based on the condition of the tooth status is necessary as a scientific and artistic technique, which has not been...  相似文献   

Early heat acclimation during incubation improves Japanese quail performance under summer conditions     

Farghly  Mohamed  Mahrose  Khalid  Abougabal  Mohammed  Taboosha  Mossad  Ali  Reham 《Veterinary research communications》2022,46(1):93-100

Effects of exposing quail eggs to high temperature on the heat tolerance ability and productivity of birds were investigated. Four groups of 600 fertile eggs were randomly selected; the first group was incubated under 37.5 °C and the hatched chicks were reared under a gradual decrease in temperature from 35 to 24 °C (Control). The second group was exposed to 39.1 °C for 2 h/day during 4–14 days of embryogenesis and the hatched chicks were reared under a gradual decrease in temperature from 35 to 24 °C. The third group was incubated under 37.5 °C and the hatched chicks were exposed to 39?±?1 °C for 2 h/day during 4–14 days of age. The fourth group was exposed to 39?±?1 °C for 2 h during 4–14 days of embryogenesis and the hatched chicks were exposed to 39?±?1 °C for 2 h/day during 4–14 days of age. The temperature applied changed (P?<?0.01) embryo weight and incubation period. Birds exposed to high temperature during brooding had superior growth performance, dressed carcass, body temperature and health traits. Birds subjected to 39?±?1 °C during brooding exhibited decreased feed consumption and body weight gain. Finally, this work suggests that thermal acclimation during embryogenesis might offer a practical method for easing heat stress.

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Antimicrobial,immunological and biochemical effects of florfenicol and garlic (Allium sativum) on rabbits infected with Escherichia coli serotype O55: H7     

Farag  Verginia M.  El-Shafei  Reham A.  Elkenany  Rasha M.  Ali  Hanaa S.  Eladl  Abdelfattah H. 《Veterinary research communications》2022,46(2):363-376

Veterinary Research Communications - Florfenicol (FFC) is a synthetic broad-spectrum antibiotic and garlic has a bactericidal action against coliforms. This study was carried out to compare the...  相似文献