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61.
Summary After a short introduction into recent collecting activities in Cuba information is provided concerning the joint expedition of the Instituto de Investigaciones Fundamentales en Agricultura Tropical and the Zentralinstitut für Genetik und Kulturpflanzenforschung in the province of Holguín, Eastern Cuba, in February 1986 within the agreement between the Academy of Sciences of Cuba and the Academy of Sciences of the German Democratic Republic. Altogether 249 accessions of vegetables, pulses, cereals and other crops were collected. A remarkable diversity was found inCapsicum andPhaseolus. The material collected is important for breeding purposes.
Sammlung pflanzlicher genetischer Ressourcen in Kuba
Zusammenfassung Nach einer kurzen Einführung in die Sammelarbeit der letzten Jahre in Kuba folgen Informationen über die gemeinsame Expedition des Instituto de Investigaciones Fundamentales en Agricultura Tropical und des Zentralinstituts für Genetik und Kulturpflanzenforschung, die im Rahmen des Abkommens zwischen der Akademie der Wissenschaften Kubas und der Akademie der Wissenschaften der Deutschen Demokratischen Republik im Februar 1986 durchgeführt wurde und in die Provinz Holguín, Ost-Kuba, führte. Insegsamt wurden 249 Proben von Gemüsen, Hülsenfrüchten, Getreiden und anderen Fruchtarten gesammelt. Eine bemerkenswerte Variation wurde beiCapsicum andPhaseolus gefunden. Das Sammelmaterial ist für Züchtungszwecke von Bedeutung.

¶rt; , , , ¶rt; . C 1986 . . 249 , , . Capsicum Phaseolus. .
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62.
Summary The principles of formal classification of cultivated plants are discussed with reference toPapaver somniferum. This species can be divided into 3 subspecies-the ancestral wild race ssp.setigerum (Dc.)Corb. and the two cultivated races ssp.somniferum and ssp.songaricum Basil. differing in geographical distribution and characters of the stigmatic lobes. In both of the cultivated races two convars are accepted on the basis of their indehiscent vs. shattering capsules. Varieties are defined according toDanert (1958) using seed- and flower-colours.Poppies, recently collected in parts of central Europe, are classified according to the proposed system. Unlike other crops, very variable land-races of poppies can still be found in this region. About 20 percent of the totally accepted varieties could be found, containing also races from ssp.songaricum and types with shattering capsules. The handling and maintaining of this material in the Gatersleben gene-bank is briefly discussed.
Probleme der Klassifikation vonPapaver somniferum und einige Bemerkungen zu unlängst gesammelten europäischen Mohn-Landsorten
Zusammenfassung Grundzüge der formalen Klassifikation von Kulturpflanzen unter besonderer Berücksichtigung vonPapaver somniferum werden diskutiert. Diese Art kann in drei Subspecies aufgegliedert werden — die wilde Ausgangssippe ssp.setigerum (DC.)Corb. und die beiden Kultursippen ssp.somniferum und ssp.songaricum Basil., die sich durch ihre geografische Verbreitung und Merkmale der Narbenscheiben voneinander abgrenzen lassen. In beiden Kultursippen werden je zwei Convarietäten unter Zugrundelegung ihres Schließ- bzw. Schüttmohncharakters unterschieden. Varietäten werden nachDanert (1958) anhand der Samen- und Blütenfarben definiert.Die in jüngster Zeit in einigen Teilen Zentraleuropas gesammelten Mohne werden nach dem vorgeschlagenen System klassifiziert. Im Gegensatz zu anderen Kulturpflanzen können in diesen Gebieten noch sehr variable Mohn-Landsorten gefunden werden. Ungefähr 20% der im System aufgeführten Varietäten konnten gesammelt werden, einschließlich einiger Vertreter der ssp.songaricum und von Schüttmohnsippen. Die Bearbeitung und Erhaltung dieses Materials in der Genbank Gatersleben wird kurz diskutiert.

Papaver somniferum
, ë Papaver somniferum. ë : — ssp.setigerum (DC.)Corb. — ssp.somniferum ssp.songaricum Basil., . (convarietas) — . , (1958) . , , . , ë . 20% , ë , ssp.songaricum . .
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63.
Zusammenfassung 490 Sippen von 21Aegilops-Arten wurden in den Jahren 1980 bis 1982 unter Freilandbedingungen mit künstlicher Infektion auf Resistenz gegenüber dem Erreger der Halmbruchkrankheit des Getreides,Pseudocercosporella herpotrichoides (Fron) Deighton geprüft. Außerdem erfolgte an ausgewählten Sippen die Prüfung der Jungpflanzenanfälligkeit im Phytotron. Unter denAegilops-Mustern erwiesen sich im Freiland 31 Herkünfte als resistent bzw. schwach anfällig. Im einzelnen handelte es sich um Vertreter der ArtenAe. ventricosa, Ae. triuncialis, Ae. kotschyi, Ae. bicornis, Ae. columnaris, Ae. lorentii, Ae. peregrina, Ae. geniculata, Ae. speltoides, Ae. crassa undAe. tauschii. Die im Jungpflanzenstadium geprüften 7 Sippen, 3 vonAe. ventricosa und je 1 vonAe. bicornis, Ae. kotschyi undAe. lorentii, waren ebenfalls resistent bzw. schwach anfällig.
TestingAegilops species for their reaction toPseudocercosporella herpotrichoides (Fron) Deighton
Summary From 1980–1982 490 accessions out of 21Aegilops species were tested in a field trial with artificial infection regarding their resistance toPseudocercosporella herpotrichoides (Fron) Deighton in cereal crops. Simultaneously, selected accessions were checked for their susceptibility in the seedling stage under controlled conditions in the phytotrone. Among theAegilops samples 31 accessions proved to be resistant or only slightly susceptible. This refers toAe. ventricosa, Ae. triuncialis, Ae. kotschyi, Ae. bicornis, Ae. columnaris, Ae. lorentii, Ae. peregrina, Ae. geniculata, Ae. speltoides, Ae. crassa andAe. tauschii. The accessions checked in the seedling stage, i. e. 3 ofAe. ventricosa and 1 ofAe. bicornis, Ae. kotschyi andAe. lorentii, showed also resistance or only low susceptibility.

Aegilops Pseudocercosporella herpotrichoides
1980–1982 . 490 21 Aegilops Pseudocercosporella herpotrichoides (Fron) Deighton. , . Aegilops 31 ; ¶rt; Ae. ventricosa, Ae. triuncialis, Ae. kotschyi, Ae. bicornis, Ae. columnaris, Ae. lorentii, Ae. peregrina, Ae. geniculata, Ae. speltoides, Ae. crassa, Ae. tauschii. , , Ae. ventricosa Ae. bicornis, Ae. kotschyi, Ae. lorentii .
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64.
Amplified Fragment Length Polymorphisms (AFLPs) were employed within the taxonomically difficult Solanum nigrum L. complex in order to characterize the genetic diversity present in a collection of the Gatersleben Genebank, to classify taxonomically unknown material, and to correlate the clustering of the examined accessions with their geographic origin. The results from AFLP analyses using two primer combinations on 44 entries from five species led to the detection of four major clusters, simultaneously uncovering significant differences in the levels of genetic diversity within or between species. S. americanumexhibited the highest infraspecific variation despite close geographic origins, simultaneously being placed in a clearly separated cluster in comparison to the other examined species of the complex. In addition, these other species showed even less interspecific variation than was found at the infraspecific level in S. americanum. In terms of taxonomy, the application of AFLPs helped in the classification of 13 black nightshade accessions formerly only listed as Solanum sp. This also was confirmed by morphological determination. Furthermore, one accession formerly classified as S. physalifolium var. nitidibaccatum i) clustered with AFLPs and ii) was identified morphologically as S. villosum. Contradictory classifications remain for two further entries from the same species, found within the S. nigrum cluster after AFLP analyses, while belonging to S. physalifolium var. nitidibaccatum according to herbarium specimen. Finally, as indicated by the information on provenance in geographically separated subclusters in S. americanum and partially in S. villosum, clues on the currently unknown origin of accessions from the genebank seem feasible by AFLP data.  相似文献   
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68.
Three dogs receiving cyclophosphamide IV as part of a combination chemotherapeutic regimen developed macrohematuria, stranguria, and pollakiuria within 24 hours of administration of the first dose of this drug. An 11-year-old spayed mixed-breed dog with an oral squamous cell carcinoma was administered 250 mg of cyclophosphamide/m2 of body surface, whereas a 4-year-old castrated male Gordon Setter was treated with 100 mg of cyclophosphamide/m2 and a 6-year-old male German Shepherd Dog with a cutaneous hemangiosarcoma was administered 140 mg of cyclophosphamide/m2. Aerobic bacterial culture, antimicrobial susceptibility testing, and urinalysis were performed on urine obtained by cystocentesis from all 3 dogs after hematuria was observed. Sterile hemorrhagic cystitis was diagnosed on the basis of large numbers of RBC in the urine, lack of pathogens on bacterial culturing of urine, and clinical signs. Although cyclophosphamide-induced cystitis in dogs has been reported in the literature numerous times, acute episodes developing within 24 hours of administration of the first dose have not been reported in this species with the use of therapeutic doses. Therefore, appropriate precautionary steps should be taken, even when the drug is being administered intermittently.  相似文献   
69.
Genetic engineering of mammalian embryos   总被引:2,自引:0,他引:2  
A gene consisting of the mouse metallothionein I promoter/regulator (MT) fused to the human growth hormone (hGH) structural gene (MThGH) was microinjected into rabbit, sheep and pig eggs. Visualization of nuclear structures was accomplished by interference-contrast (I-C) microscopy for rabbit and sheep eggs and by centrifugation and I-C microscopy for pig eggs. The gene integrated into the chromosomes of each species with an efficiency of 13% in rabbits, 1% in sheep and 10% in pigs. Human GH mRNA was detected in the liver of transgenic rabbits as well as tail and ear samples of pigs. Immunoreactive hGH was present in the serum of a transgenic rabbit and plasma of most transgenic pigs. In several pigs hGH levels increased between birth and 90 d of age. The presence of substantial quantities of hGH in plasma of pigs did not increase postnatal somatic growth rates. Founder animals will be bred and their transgenic and control progeny used to assess the effects of hGH on feed efficiency and carcass composition. These experiments demonstrate the feasibility of introducing foreign genes into the genome of several animal species by microinjection of eggs.  相似文献   
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