Benzothiazole-based semisquaraine as colorimetric chemosensor for Hg2+     

Jin-Seok Bae  Young-A Son  Sung-Hoon Kim 《Fibers and Polymers》2009,10(3):403-405

A highly selective colorimetric chemosensor for Hg²⁺ based on the semisquaraine (BSQ) containing benzothiazole moiety was investigated. BSQ showed color change only with Hg²⁺, but it showed no significant changes upon addition of other metal ions such as Ca²⁺, Pb²⁺, Al³⁺, Ce²⁺, Ba²⁺, Ni²⁺, Cd²⁺, Zn²⁺, and Mg²⁺. Hg²⁺ ions coordinated to the BSQ forming 1:1 complex. The experimental data and conclusions are rationalized by DMol³ calculation results.  相似文献   

Modification of polypropylene fibers by electron beam irradiation. I. Evaluation of dyeing properties using cationic dyes     

Hongje Kim  Jin-Seok Bae 《Fibers and Polymers》2009,10(3):320-324

The dyeing properties of hydrophobic polypropylene fibers using cationic dyes were investigated to improve dyeability by electron beam irradiation and sulfonic acid incorporation. The color strength of polypropylene fibers after irradiation was examined according to the dyeing conditions including the pH of the dyebath, absorbed doses, and the introduction of a functional group to the fiber substrate. The best dyeing result was obtained when polypropylene fibers incorporated by sulfonic acid group after electron beam irradiation were dyed with cationic dyes at alkaline conditions and 30∼75 kGy irradiation ranges.  相似文献   

Colorimetric signaling of mono-, di-, and triethylamine based on intermolecular n-π charge transfer interaction     

Seon-Yeong Gwon  Jin-Seok Bae  Young-A Son  Sheng Wang  Sung-Hoon Kim 《Fibers and Polymers》2009,10(6):855-857

Intermolecular charge-transfer (CT) complexes between dichloronaphthoquinone (DCN) as acceptor and amine derivatives as donors such as ethylamine, diethyl-amine, triethyl-amine were investigated using spectroscopic techniques. The colorimetric signaling of CT complex from the point of view of their energy levels was carried out by theory calculation. The CT complexes were highly colored with ethylamine, diethyl-amine, triethyl-amine sensing giving rise to a colorless to yellow, red, and blue, respectively. A simple “OR” logic operation was successfully realized by ethylamine, diethyl-amine as input signals. Due to the dependence of the CT complexes absorption intensity of added ethylamine and diethyl-amine, it is possible to mimic the function of the truth table of an “OR” gate.  相似文献   

Multiplex Real-Time Quantitative PCR to Detect and Quantify Verticillium dahliae Colonization in Potato Lines that Differ in Response to Verticillium Wilt     

Atallah ZK  Bae J  Jansky SH  Rouse DI  Stevenson WR 《Phytopathology》2007,97(7):865-872

ABSTRACT Potato early dying (PED), also known as Verticillium wilt, caused by Verticillium dahliae, is a seasonal yield-limiting disease of potato worldwide, and PED-resistant cultivars currently represent only a small percentage of potato production. In this study, we developed a real-time quantitative polymerase chain reaction (Q-PCR) approach to detect and quantify V. dahliae. The efficiency of the designed primer pair VertBt-F/VertBt-R, derived from the sequence of the beta-tubulin gene, was greater than 95% in monoplex Q-PCR and duplex (using Plexor technology) procedures with primers PotAct-F/PotAct-R, obtained from the sequence of the actin gene, designed for potato. As few as 148 fg of V. dahliae DNA were detected and quantified, which is equivalent to five nuclei. Q-PCR detected V. dahliae in naturally infected air-dried potato stems and fresh stems of inoculated plants. Spearman correlations indicated a high correlation (upward of 80%) between V. dahliae quantifications using Q-PCR and the currently used plating assays. Moreover, Q-PCR substantially reduced the variability compared with that observed in the plating assay, and allowed for the detection of V. dahliae in 10% of stem samples found to be pathogen free on the culture medium. The described Q-PCR approach should provide breeders with a more sensitive and less variable alternative to time-consuming plating assays to distinguish response of breeding lines to colonization by V. dahliae.  相似文献   

Specific labeling of neurogenic, endothelial, and myogenic differentiated cells derived from human amniotic fluid stem cells with silica-coated magnetic nanoparticles     

Lee JK  Chun SY  Im JY  Jin HK  Kwon TG  Bae JS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(8):969-975

Stem cell based cell therapies offer significant potential for the field of regenerative medicine. Human amniotic fluid stem cells (hAFSCs) are an attractive source for lineage-specific differentiated stem cell therapy since they have properties that are able to differentiate into cells representing all three germ layers. To better understand the fate and location of implanted hAFSCs, a means to monitor cells in living subjects is essential. Here, we showed that differentiated cells, such as neurogenic, endothelial, and myogenic cells, derived from hAFSCs can be effectively labeled by the FITC-incorporated silica-coated nanoparticles, MNPs@SiO2 (FITC), although the labeling efficacy and cytotoxicity were distinct depending on the differentiated cell type. In addition, we observed that MNPs@SiO2-labeled cells provided sufficient signals for detection by optical and confocal microscope imaging when transplanted into the mice. These results suggest that the fluorescent dye incorporated MNPs@SiO2 are a useful tool for the cell labeling and in vivo tracking of differentiated cells derived from hAFSCs.  相似文献   

Genetic and pathogenic characterization of Akabane viruses isolated from cattle with encephalomyelitis in Korea     

Oem JK  Yoon HJ  Kim HR  Roh IS  Lee KH  Lee OS  Bae YC 《Veterinary microbiology》2012,158(3-4):259-266

A large-scale outbreak of Akabane viral encephalomyelitis in cattle was reported in the southern part of Korea in 2010. Fifteen Akabane virus (AKAV) strains were isolated from the brain and spinal cord samples by using BHK-21 and/or HmLu-1 cells. To examine the genetic relationships and characteristics of the isolates, nucleotide sequences of the S, M, and L segments of the 15 isolates were determined and analyzed. Complete sequence analysis of the 15 AKAV isolates showed 99.9-100% amino acid identities, indicating that the 15 isolates originated from a single strain. The S and M RNA segments of a representative isolate (AKAV-7/SKR/2010) were also compared with the segments of representative reference sequences. This AKAV-7/SKR/2010 strain showed the highest identity with the Iriki and KM-1/Br/06 strains. Neighbor-joining phylogenetic trees of S and M RNA segments were constructed. Four representative AKAV isolates were classified into subgroup Ia, which contains the Iriki and KM-1/Br/06 strains recognized to cause encephalomyelitis in calves and adult cattle in Japan. Moreover, experimental intraperitoneal infection was performed using the AKAV-7/SKR/2010 and AKAV-17/SKR/2010 strains to assess pathogenesis in suckling mice. The 2 isolates, genetically related to the Iriki strain, were neurovirulent and caused neurological signs in suckling mice. In contrast, the 93FMX strain and the K0505 strain, related to the OBE-1 strain, were avirulent in mice. The present results indicate that these isolates most likely had originated from the Iriki strain and are closely related to the Iriki strain both genetically and pathogenically.  相似文献   

Genotypic prevalence of the fimbrial adhesins (F4, F5, F6, F41 and F18) and toxins (LT, STa, STb and STx2e) in Escherichia coli isolated from postweaning pigs with diarrhoea or oedema disease in Korea     

Kwon D  Choi C  Jung T  Chung HK  Kim JP  Bae SS  Cho WS  Kim J  Chae C 《The Veterinary record》2002,150(2):35-37

A PCR was used to determine the genotypic prevalence of five fimbrial adhesins (F4, F5, F6, F41 and F18), two heat-stable enterotoxins (STa and STb), the heat-labile enterotoxin (LT), and the shiga toxin 2e (Stx2e) in 230 isolates of Escherichia coli from postweaning pigs with diarrhoea or oedema disease. Ninety-four (40.9 per cent) of the isolates carried genes for at least one of the fimbrial adhesins or toxins. Genes for the F18 fimbrial adhesin were detected in 18.3 per cent, and genes for F4, F6, F5 and F41 were detected in 10.0 per cent, 4.3 per cent, 1.7 per cent and 0.8 per cent of the isolates, respectively. Genes for STa, STb and LT were detected in 25.7 per cent, 15.2 per cent and 8.7 per cent of the isolates, respectively. Genes for Stx2e were detected in 36 (15.6 per cent) of the isolates, and among them 24 also contained the gene for F18ab and four also contained the gene for F18ac.  相似文献   

Therapeutic monitoring of rivaroxaban in dogs using thromboelastography and prothrombin time     

Junwoo Bae  Hyunwoo Kim  Woosun Kim  Suhee Kim  Jinho Park  Dong‐In Jung  Dohyeon Yu 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(3):1322-1330

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Feed contaminated with classical swine fever vaccine virus (LOM strain) can induce antibodies to the virus in pigs     

Kim B  Song JY  Tark DS  Lim SI  Choi EJ  Kim J  Park CK  Lee BY  Wee SH  Bae YC  Lee OS  Kwon JH  Kang WC  Kim TY  Kim JH  Lee JH  Kang MI 《The Veterinary record》2008,162(1):12-17

In November 2004, antibodies to classical swine fever virus (csfv) were detected in finishing pigs during the annual serological surveillance in Jeju Province, Korea. In addition, csf vaccine viruses (lom strain) had recently been isolated from pigs raised on farms known to have csfv antibody-positive pigs. In contrast with mainland Korea, Jeju Province had been csf free and its pigs had not been vaccinated against csf for more than five years. An epidemiological investigation team from the National Veterinary Research and Quarantine Service investigated the current status of csf prevention on the Korean mainland and in Jeju Province to determine possible routes of introduction of the virus into the province. It was concluded that improperly processed blood meals, manufactured on mainland Korea, had been contaminated with the csf vaccine lom strain, and that the lom strain had been transmitted to pigs fed feed or feedstuffs containing the contaminated meal.  相似文献   

Pyelonephritis associated with Staphylococcus intermedius in a Siberian tiger (Panthera tigris altaica)     

Jee H  Pakhrin B  Bae IH  Shin NS  Lee SI  Yoo HS  Kim DY 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(8):851-852

Pyelonephritis, in which Staphylococcus intermedius was isolated, was diagnosed in a 4-year-old female Siberian tiger (Panthera tigris altaica). At necropsy, the renal pelvis was dilated with a large amount of purulent exudates. Microscopically, the lesions of renal pelvis and medulla consisted of necrotic foci intermingled primarily with numerous degenerative neutrophils and a few lymphocytes, plasma cells and macrophages. Bacteriology showed the presence of S. intermedius. This is the first report on pyelonephritis associated with S. intermedius in wild felidae.  相似文献