A serine proteinase from the sarcoplasmic fraction of red sea bream Pagrus major is possibly derived from blood     

Asami Yoshida  Inwoo Bae  Hiroko Sonoda  Min-Jie Cao  Katsuyasu Tachibana  Kiyoshi Osatomi  Kenji Hara 《Fisheries Science》2009,75(6):1439-1444

Collagen degradation is known to be involved in the post mortem tenderization of fish muscle. A serine proteinase that is assumed to be related to collagen degradation after fish death was purified from the sarcoplasmic fraction of red sea bream Pagrus major by ammonium sulfate fractionation and column chromatography on Sephacryl S-300, Q Sepharose and Phenyl Sepharose CL-4B. The enzyme hydrolyzed gelatin and was obtained as a protein band of approximately 38 kDa upon sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing conditions. The N-terminal amino acid sequence of the enzyme was determined for 32 residues. A protein that had the same N-terminal amino acid sequence as the enzyme for ten residues was purified from the serum of red sea bream and showed the same characteristics as the enzyme. Therefore, it is suggested that the serine proteinase migrates from the blood to muscle and degrades muscle proteins after the death of the fish.  相似文献   

Binding,Antioxidant and Anti-proliferative Properties of Bioactive Compounds of Sweet Paprika (Capsicum annuum L.)     

Hong-Gi Kim  Jong-Hyang Bae  Zenon Jastrzebski  Andriy Cherkas  Buk-Gu Heo  Shela Gorinstein  Yang-Gyu Ku 《Plant foods for human nutrition (Dordrecht, Netherlands)》2016,71(2):129-136

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Successful treatment of canine infective endocarditis caused by Bacillus amyloliquefaciens     

Hyeona Bae  Tae-Sung Hwang  Hee-Chun Lee  Dong-In Jung  Sang-Hyun Kim  DoHyeon Yu 《The Veterinary quarterly》2022,42(1):41

Bacillus amyloliquefaciens is a gram-positive bacterial species that is utilised as a probiotic in humans and animals. There are no reports of infective endocarditis (IE) in dogs. An 8-year-old, spayed, female Maltese presented with a 1-month history of fever, depression, weight loss, and hindlimb lameness. Laboratory test results indicated non-regenerative anaemia, neutrophilia, hyperglobulinemia, and proteinuria. Echocardiography revealed vegetation on the septal leaflet of the mitral valve and thromboemboli in the left atrium. Consecutive blood culture results revealed that the blood samples were consistently positive for Bacillus amyloliquefaciens, which is generally considered a probiotic bacterial species for animals. Broad-spectrum antibiotics (amoxicillin-clavulanic acid and cefotaxime) and anticoagulants (clopidogrel and rivaroxaban) were administered for 4 months. The clinical signs were responsive to antibiotic treatment. After 4 months, the dog was no longer febrile and the size of the thromboemboli in the left atrium had decreased. Bacteria were no longer isolated in blood cultures after antibiotic therapy. To the best of our knowledge, this is the first case report of canine IE caused by bactaeremic infection with Bacillus amyloliquefaciens.  相似文献   

The response of U.S. regional demographics to import shocks     

Seung‐hun Chung  Jung Dae Bae 《Growth and change》2019,50(3):969-1005

We analyze regional demographic responses to increased competition from Chinese imports. Treating the opening up of the Chinese economy in recent decades as an exogenous shock to the degree of trade exposure faced by U.S. regions, we find that demographic characteristics such as education level, population growth, immigrant share, and age structure, are not significantly impacted by increased Chinese trade exposure. We also discuss possible mechanisms that may explain this finding and explain why our results may differ in direction from previous studies. Specifically, our finding is consistent with a story where moving costs hamper labor mobility and significant demographic shifts do not occur even after employment falls due to trade exposure.  相似文献   

Echinacea species and alkamides inhibit prostaglandin E(2) production in RAW264.7 mouse macrophage cells   总被引：1，自引：0，他引：1  

LaLone CA  Hammer KD  Wu L  Bae J  Leyva N  Liu Y  Solco AK  Kraus GA  Murphy PA  Wurtele ES  Kim OK  Seo KI  Widrlechner MP  Birt DF 《Journal of agricultural and food chemistry》2007,55(18):7314-7322

Inhibition of prostaglandin E(2) (PGE(2)) production in lipopolysaccharide-stimulated RAW264.7 mouse macrophage cells was assessed with an enzyme immunoassay following treatments with Echinacea extracts or synthesized alkamides. Results indicated that ethanol extracts diluted in media to a concentration of 15 microg/mL from E. angustifolia, E. pallida, E. simulata, and E. sanguinea significantly inhibited PGE2 production. In further studies, PGE2 production was significantly reduced by all synthesized alkamides assayed at 50 microM, by Bauer alkamides 8, 12A analogue, and 14, Chen alkamide 2, and Chen alkamide 2 analogue at 25 microM and by Bauer alkamide 14 at 10 microM. Cytotoxicity did not play a role in the noted reduction of PGE2 production in either the Echinacea extracts or synthesized alkamides. High-performance liquid chromatography analysis identified individual alkamides present at concentrations below 2.8 microM in the extracts from the six Echinacea species (15 microg/mL crude extract). Because active extracts contained <2.8 microM of specific alkamide and the results showed that synthetic alkamides must have a minimum concentration of 10 microM to inhibit PGE2, it is likely that alkamides may contribute toward the anti-inflammatory activity of Echinacea in a synergistic or additive manner.  相似文献   

Characterization of storage proteins in wild (Glycine soja) and cultivated (Glycine max) soybean seeds using proteomic analysis   总被引：1，自引：0，他引：1  

Natarajan SS  Xu C  Bae H  Caperna TJ  Garrett WM 《Journal of agricultural and food chemistry》2006,54(8):3114-3120

A combined proteomic approach was applied for the separation, identification, and comparison of two major storage proteins, beta-conglycinin and glycinin, in wild (Glycine soja) and cultivated (Glycine max) soybean seeds. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) with three different immobilized pH gradient (IPG) strips was an effective method to separate a large number of abundant and less-abundant storage proteins. Most of the subunits of beta-conglycinin were well-separated in the pH range 3.0-10.0, while acidic and basic glycinin polypeptides were well-separated in pH ranges 4.0-7.0 and 6.0-11.0, respectively. Although the overall distribution pattern of the protein spots was similar in both genotypes using pH 3.0-10.0, variations in number and intensity of protein spots were better resolved using a combination of pH 4.0-7.0 and pH 6.0-11.0. The total number of storage protein spots detected in wild and cultivated genotypes was approximately 44 and 34, respectively. This is the first study reporting the comparison of protein profiles of wild and cultivated genotypes of soybean seeds using proteomic tools.  相似文献   

Outbreak of pandemic influenza (H1N1) 2009 in pigs in Korea     

Kim SH  Moon OK  Lee KK  Song YK  Yeo CI  Bae CW  Yoon H  Lee OS  Lee JH  Park CK 《The Veterinary record》2011,169(6):155

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Intestinal and extra-intestinal pathogenicity of a bovine reassortant rotavirus in calves and piglets     

Kim HJ  Park JG  Matthijnssens J  Lee JH  Bae YC  Alfajaro MM  Park SI  Kang MI  Cho KO 《Veterinary microbiology》2011,152(3-4):291-303

Despite the impact of bovine group A rotaviruses (GARVs) as economically important and zoonotic pathogens, there is a scarcity of data on cross-species pathogenicity and extra-intestinal spread of bovine reassortant GARVs. During the course of characterizing the genotypes of all 11 genomic segments of bovine GARVs isolated from diarrheic calves in South Korea, a unique G6P[7] reassortant GARV strain (KJ9-1) was isolated. The strain harbors five bovine-like gene segments (VP7: G6; VP6: I2; VP1: R2; VP3: M2; NSP2: N2, and NSP4: E2), five porcine-like gene segments (VP4: P[7]; NSP1: A1; NSP3: T1, and NSP5: H1), and one human-like gene segment (VP2: C2). To investigate if this reassortant strain possessed cross-species pathogenicity in calves and piglets, and could induce viremia and extra-intestinal spread in calves, colostrum-deprived calves and piglets were experimentally inoculated with the KJ9-1 strain. The KJ9-1 strain caused severe diarrhea in experimentally infected calves with extensive intestinal villous atrophy, but replicated without causing clinical symptoms in experimentally infected piglets. By SYBR Green real-time RT-PCR, viral RNA was detected in sera of the calves at post-inoculation day (PID) 1, reaching a peak at PID3, and then rapidly decreasing from PID4. In addition, viral RNA was detected in the mesenteric lymph node, lungs, liver, choroid plexus, and cerebrospinal fluid. An immunofluorescence assay confirmed viral replication in the extra-intestinal organs and tissues of virus-inoculated calves. The data indicates that the homologous/heterologous origin of the NSP4 gene segment (E2 genotype), may play a key role in the ability to cause diarrhea in calves and piglets.  相似文献   

Natural occurrence of grape poisoning in two dogs     

Yoon SS  Byun JW  Kim MJ  Bae YC  Shin YK  Yoon S  Lee G  Song JY 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(2):275-277

Clinical grape poisoning in two dogs (a 1.6-year-old male Shih Tzu and a 5-year-old female Yorkshire Terrier) was described in the present study. Clinical signs included decreased urine output in the Shih Tzu and ataxia in the Yorkshire Terrier after grape ingestion. The Shih Tzu died 5 days post-grape ingestion, while the Yorkshire Terrier died 3 days post-grape ingestion. Erythematous serosae and mucosae, multifocal red small intestinal foci, and blood and grape seeds were identified in the intestinal lumen. Brownish-yellow crystals were bilaterally identified in the renal pelvis. The primary histological findings were acute tubular necrosis of the proximal convoluted tubules, severe necrosis, and mineralization in the renal cortical tubules. Blood urea nitrogen, creatinine, and alanine aminotransferase were increased in the dogs. Many Korean veterinary clinicians have suspected clinical grape poisoning. However, to our knowledge, grape poisoning has not been identified by pathologic and clinicopathologic basis until this writing in Korea. Education and knowledge about the risks of grape poisoning is necessary for the prevention of accidental exposures.  相似文献   

Antioxidant properties and structural analysis of phenolic glucosides from bark of <Emphasis Type="Italic">Populus ussuriensis</Emphasis> Kom.     

Chuan-Ling Si  Jin-Kyu Kim  Young-Soo Bae  Peng-Tao Liu  Zhong Liu 《Wood Science and Technology》2011,45(1):5-13

The methanol (MeOH) extract of Populus ussuriensis Kom. bark was analyzed for antioxidant assessing by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging potential. Among fractions using several solvents, the ethyl acetate (EtOAc) soluble fraction, which showed strong antioxidant activity (IC₅₀ 2.02 ± 0.01 μg/ml), was further purified by Thin layer chromatography (TLC) guided Sephadex LH-20 column chromatography. Three known phenolic glucosides, picein (I), salicortin (II), grandidentatin (III), and that of a new, 2-hydroxycyclohexyl-4′-O-p-coumaroyl-β-d-glucopyranoside (isograndidentatin A), were isolated and their structures were elucidated on the basis of physiochemical and spectroscopic methods. This was the first report of the chemical composition of P. ussuriensis bark. Phenolic glucosides III and IV exhibited strong antioxidant activities, with IC₅₀ values 6.73 ± 0.01 and 6.69 ± 0.01 μM, respectively, comparable to the control (α-tocopherol, IC₅₀ 6.80 ± 0.01 μM). P. ussuriensis bark EtOAc-soluble fraction and compounds III and IV could be used as biopreservatives in food applications as well as for cosmetic and medicinal preparations, to alleviate oxidative stress.  相似文献