首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   561篇
  免费   23篇
林业   20篇
农学   12篇
基础科学   2篇
  89篇
综合类   46篇
农作物   27篇
水产渔业   49篇
畜牧兽医   310篇
园艺   9篇
植物保护   20篇
  2023年   3篇
  2022年   4篇
  2021年   2篇
  2020年   11篇
  2019年   7篇
  2018年   12篇
  2017年   11篇
  2016年   10篇
  2015年   9篇
  2014年   9篇
  2013年   47篇
  2012年   36篇
  2011年   24篇
  2010年   19篇
  2009年   24篇
  2008年   19篇
  2007年   22篇
  2006年   22篇
  2005年   24篇
  2004年   23篇
  2003年   34篇
  2002年   14篇
  2001年   11篇
  2000年   18篇
  1999年   19篇
  1998年   5篇
  1997年   2篇
  1996年   2篇
  1995年   11篇
  1994年   8篇
  1993年   5篇
  1992年   14篇
  1991年   7篇
  1990年   4篇
  1989年   8篇
  1988年   8篇
  1987年   4篇
  1986年   10篇
  1985年   6篇
  1984年   3篇
  1983年   13篇
  1982年   3篇
  1981年   4篇
  1980年   2篇
  1979年   9篇
  1978年   4篇
  1977年   4篇
  1976年   4篇
  1974年   2篇
  1973年   2篇
排序方式: 共有584条查询结果,搜索用时 468 毫秒
141.
Interspecific interaction among sympatric ungulates is important in management and conservation. We investigated behavioral interference between sympatric wild or semidomestic reindeer (Rangifer tarandus tarandus) and sheep (Ovis aries) in two field studies and one enclosure experiment. For free-ranging wild and semidomestic reindeer, interference between the two species increased with decreasing distances, occurring only at less than 200 m and 30 m, for wild and semidomestic reindeer, respectively, and neither species consistently dominated the other. In a controlled, duplicated experiment we tested interference and confrontations at the feeding patch level among semidomestic reindeer and sheep within 40 × 50 m enclosures. When new reindeer or sheep were introduced into enclosures already occupied by reindeer, new reindeer resulted in significantly more interference and confrontations among individuals compared to new sheep; i.e., intraspecific interference was more prevalent than interspecific interference at equal densities. For all study areas, confrontations decreased with time after “first encounter,” indicating cohabituation. A sympatric use of pastures was not visually disruptive for recorded grazing behavior for either species.  相似文献   
142.
The purpose of our work was to establish an efficient-oriented enucleation method to produce transgenic embryos with handmade cloning (HMC). After 41–42 h oocytes maturation, the oocytes were further cultured with or without 0.4 μg/ml demecolcine for 45 min [chemically assisted handmade enucleation (CAHE) group vs polar body (PB) oriented handmade enucleation (OHE) group respectively]. After removal of the cumulus cells and partial digestion of the zona pellucida, oocytes with visible extrusion cones and/or polar bodies attached to the surface were subjected to oriented bisection. Putative cytoplasts without extrusion cones or PB were selected as recipients. Two cytoplasts were electrofused with one transgenic fibroblasts expressing green fluorescent protein (GFP), while non-transgenic fibroblasts were used as controls. Reconstructed embryos were cultured in Well of Wells (WOWs) with porcine zygote medium 3 (PZM-3) after activation. Cleavage and blastocyst rates were registered on day 2 and day 7 of in vitro culture respectively. Meanwhile, the total blastocyst cell number was counted on day 7. We found that the difference was only observed between blastocyst rates (38.6 ± 2% vs 48.1 ± 3%) of cloned embryos with GFP transgenic fibroblast cells after CAHE vs OHE. With adjusted time-lapse for zonae-free cloned embryos cultured in WOWs with PZM-3, it was obvious that in vitro developmental competence after CAHE was compromised when compared with the OHE method. OHE enucleation method seems to be a potential superior alternative method used for somatic cell nuclear transfer (SCNT) with transgenic fibroblast cells.  相似文献   
143.
144.
In this study we assess the economic profitability of implementing various precision farming (PF) technologies and controlled traffic farming (CTF) on 4 main crops in Denmark. The study provides insight into technological requirements, and identifies related costs and benefits to farmers and the Danish society as a whole. At the farm level, the findings from the study indicate that an implementation of CTF systems may have a significant impact on fuel savings due to a reduced overlap with auto guidance systems and easier movement with tractors and tools in the field. The PF site-specific weed management approach may facilitate large savings in the use of herbicides. At the national level, the results clearly show that the benefits to the Danish economy of adopting this new technology is positive with increased income to farmers and a reduction in fuel consumption and pesticides/herbicides use. The obtained results reflect the long term economic effect of implementing new production methods in Denmark where soil fertility increases over time due to reduced traffic increasing yields. Over all, the Danish GDP is estimated to increase by 34?million?? due to the implementation of PF and CTF on larger farms in Denmark. The results also clearly show that adoption of PF and CTF farming systems will benefit the environment. The agricultural sectors input of environmental harmful inputs are reduced in all scenarios presented in this paper. The adoption of PF and CTF systems should thus be of interest to politicians since it clearly facilitates aims of environmental policies currently under implementation by the Danish government.  相似文献   
145.
1引言 配种和轻胎舍是母猪场中最重要的一个组成部分.因此,它必须能带来高妊娠率、高产仔、均衡的体况、较长的母猪使用寿命和低返情率.也就是说,配种和轻胎舍的设计必须有助于管理者获得较高的生产率.  相似文献   
146.
147.
The purpose of this prospective study was to investigate platelet function using in vitro tests based on both high and low shear rates and von Willebrand factor (vWf) multimeric composition in dogs with cardiac disease and turbulent high-velocity blood flow. Client-owned asymptomatic, untreated dogs were divided into 4 groups: 14 Cavalier King Charles Spaniels (Cavaliers) with mitral valve prolapse (MVP) and no or minimal mitral regurgitation (MR), 17 Cavaliers with MVP and moderate to severe MR, 14 control dogs, and 10 dogs with subaortic stenosis (SAS). Clinical examinations and echocardiography were performed in all dogs. PFA100 closure times (the ability of platelets to occlude a hole in a membrane at high shear rates), platelet activation markers (plasma thromboxane B2 concentration, platelet surface P-selectin expression), platelet aggregation (in whole blood and platelet-rich plasma with 3 different agonists), and vWf multimers were analyzed. Cavaliers with moderate to severe MR and dogs with SAS had longer closure times and a lower percentage of the largest vWf multimers than did controls. Maximal aggregation responses were unchanged in dogs with SAS but enhanced in Cavaliers with MVP (regardless of MR status) compared with control dogs. No significant difference in platelet activation markers was found among groups. The data suggest that a form of platelet dysfunction detected at high shear rates was present in dogs with MR and SAS, possibly associated with a qualitative vWf defect. Aggregation results suggest increased platelet reactivity in Cavaliers, but the platelets did not appear to circulate in a preactivated state in either disease.  相似文献   
148.
Insulin-like growth factor-1 (IGF-1), which mediates most effects of growth hormone, has effects on cardiac mass and function, and plays an important role in the regulation of vascular tone. In humans, an inverse relationship between degree of heart failure (HF) and circulating IGF-1 concentrations has been found in several studies. In dogs with HF, few studies have focused on IGF-1. We examined circulating IGF-1 concentrations in dogs with mitral regurgitation (MR) caused by myxomatous mitral valve disease. Study 1 included 88 Cavalier King Charles Spaniels (CKCSs) with a broad range of asymptomatic MR (median serum IGF-1: 76.7 microg/L; 25-75 percentile, 59.8-104.9 microg/L). As expected, standard body weight and percentage under- or overweight correlated directly with IGF-1. MR (assessed in 4 different ways) did not correlate with IGF-1. In study 2, 28 dogs with severe MR and stable, treated congestive HF had similar serum IGF-1 concentrations (median, 100.8 g/L; 25-75 percentile, 74.9-156.5 microg/L) as 11 control dogs (79.6 microg/L; 25-75 percentile, 64.1-187.4 microg/L; P = .84). In study 3, the plasma IGF-1 concentration of 15 untreated CKCSs with severe MR was 16.4 +/- 24.2 microg/L lower (P = .02) at the examination when decompensated HF had developed (80.8 +/- 30.9 microg/L) than at a visit 1-12 months earlier (97.2 +/- 39.8 microg/L), possibly in part due to an altered state of nutrition. The studies document that circulating IGF-1 concentrations are not altered before development of congestive HF in dogs with naturally occurring MR, but decrease by approximately 20% with the development of untreated HE In treated HF, circulating IGF-1 concentrations apparently return to within the reference range.  相似文献   
149.
Lymphoma (lymphosarcoma or malignant lymphoma) is the most common neoplasm of the hematopoietic system of cats and reportedly the cat has the highest incidence for lymphoma of any species. A 21-year retrospective survey of feline lymphoma covering the period 1983-2003 was conducted with the patient database at the Veterinary Medicine Teaching Hospital (VMTH) at the University of California, Davis, School of Veterinary Medicine. This period comprises the post-feline leukemia virus (FeLV) era. Feline lymphoma historically has been highly associated with retrovirus infection. Mass testing and elimination and quarantine programs beginning in the 1970s and vaccination programs in the 1980s dramatically reduced the subsequent FeLV infection rate among pet cats. The results of this survey confirm a significant decrease in the importance of FeLV-associated types of lymphoma in cats. In spite of this decrease in FeLV infection, the incidence of lymphoma in cats treated at the VMTH actually increased from 1982 to 2003. This increase was due largely to a rise in the incidence of intestinal lymphoma, and to a lesser degree, of atypical lymphoma. A high incidence of mediastinal lymphomas in young Siamese or Oriental breeds also was observed, supporting previous studies. Associations of intestinal lymphoma and inflammatory bowel disease and diet should be further considered.  相似文献   
150.
OBJECTIVE: To evaluate markers of hemostasis and their relationship to the degree of mitral regurgitation (MR) and platelet function in Cavalier King Charles Spaniels (CKCSs) with myxomatous mitral valve disease. ANIMALS: 76 clinically healthy CKCSs and 24 control dogs. PROCEDURE: All dogs underwent echocardiographic examination; various hemostatic, hematologic, and biochemical variables were evaluated in blood. The CKCSs were allocated to 1 of 3 groups on the basis of MR severity. In 8 control dogs and 8 CKCSs, plasma von Willebrand factor (vWF) multimer analysis was performed. RESULTS: Compared with control dogs, plasma fibrinogen concentration was higher in all CKCSs and related to left ventricular end diastolic diameter and left atrial-to-aortic root ratio among all CKCSs. The activated partial thromboplastin times and plasma D-dimer concentration were similar among the 4 groups. Plasma vWF concentration was lower in CKCSs with moderate to severe MR, compared with that of CKCSs with no MR and control dogs. There was a relationship between plasma vWF concentration and platelet function in CKCSs but not in control dogs. In 4 CKCSs with moderate to severe MR and low plasma vWF concentration, amounts of vWF high-molecular-weight multimers (HMWMs) were low. CONCLUSIONS AND CLINICAL RELEVANCE: In CKCSs, MR appeared to be associated with a low plasma vWF concentration and likely a loss of vWF HMWMs (possibly through their destruction via shear stress to the blood). The importance of the changes in plasma fibrinogen concentration and the thromboembolic risk in dogs with MR remain to be investigated.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号