Linking fluorescence induction curve and biomass in herbicide screening     

Christensen MG  Teicher HB  Streibig JC 《Pest management science》2003,59(12):1303-1310

A suite of dose-response bioassays with white mustard (Sinapis alba L) and sugar beet (Beta vulgaris L) in the greenhouse and with three herbicides was used to analyse how the fluorescence induction curves (Kautsky curves) were affected by the herbicides. Bentazone, a photosystem II (PSII) inhibitor, completely blocked the normal fluorescence decay after the P-step. In contrast, fluorescence decay was still obvious for flurochloridone, a PDS inhibitor, and glyphosate, an EPSP inhibitor, which indicated that PSII inhibition was incomplete. From the numerous parameters that can be derived from OJIP-steps of the Kautsky curve the relative changes at the J-step [Fvj = (Fm - Fj)/Fm] was selected to be a common response parameter for the herbicides and yielded consistent dose-response relationships. Four hours after treatment, the response Fvj on the doses of bentazone and flurochloridone could be measured. For glyphosate, the changes of the Kautsky curve could similarly be detected 4 h after treatment in sugar beet, but only after 24 hs in S alba. The best prediction of biomass in relation to Fvj was found for bentazone. The experiments were conducted between May and August 2002 and showed that the ambient temperature and solar radiation in the greenhouse could affect dose-response relationships. If the Kautsky curve parameters should be used to predict the outcome of herbicide screening experiments in the greenhouse, where ambient radiation and temperature can only partly be controlled, it is imperative that the chosen fluorescence parameters can be used to predict accurately the resulting biomass used in classical bioassays.  相似文献   

Methods for determining the vapour pressure of active ingredients used in crop protection. Part V: Thermogravimetry combined with solid phase microextraction (SPME)     

Thomas Krhl  Roland Kstel  Werner Knig  Hans Ziegler  Harald Khle  Adolf Parg 《Pest management science》1998,53(4):300-310

The well-established evaporation rate method for determining the vapour pressure of active ingredients in crop protection down to the order of 10^-12 mbar can be supplemented by the new sample preparation technique of Solid Phase MicroExtraction (SPME). With this technique, it is possible to identify evaporating substances by analysis after partitioning into the polymer coating of a thin fibre in the outlet-gas flow of thermogravimetric equipment. The active ingredients fenpropimorph, kresoxim-methyl, metolachlor, clomazone and (Z)-9-dodecenyl acetate were used in this study, which showed that, despite the relatively small amount of collected material, an analytical identification of the evaporating compound by SPME/GC can be successfully achieved. In particular, the experiments have demonstrated a clear correlation between the linearity of the weight loss curve and the evaporation process of a pure compound. In the case of organic compounds that are unstable to heat, the SPME method can also be utilized to show whether, and at what temperatures, decomposition of the sample into fragments of higher volatility occurs. For example, the insecticide dimethoate showed a clear temperature dependence of both evaporation behaviour and in the SPME/GC analysis. © 1998 SCI  相似文献   

Modelling the effects of mulching and fallow cropping on water balance in the Chinese Loess Plateau   总被引：4，自引：0，他引：4  

Shulan Zhang  Lars Lvdahl  Harald Grip  Per-Erik Jansson  Yanan Tong 《Soil & Tillage Research》2007,93(2):283-298

To improve water use efficiency on drylands it is essential to understand the mechanisms affecting water balance partitioning in arable land ecosystems. A field experiment was conducted, from October 2001 to October 2004, to evaluate the effects of field management regimes on water balance and water use efficiency in a winter wheat (Triticum aestivum L.) system in the Loess Plateau, China. The field management regimes tested were: (i) conventional agricultural management (winter wheat followed by a ploughed summer fallow); (ii) conventional management plus a fallow crop used as green manure; (iii) application of wheat straw mulch (0.8 kg m⁻²), which was retained throughout the year (no summer ploughing). A process-oriented ecosystem model (CoupModel) was calibrated with field measurements of soil water contents, leaf area indices, plant heights and crop yields, then used to generate comparative simulations of the water balance partitioning under the wheat straw mulch, fallow crop and conventional management regimes. The simulations indicated that during the experimental period mulching increased soil water storage by 5–8%, decreased soil evaporation by 11–13%, and increased wheat transpiration by 2–5% compared with the conventional management regime, thus increasing the wheat yield and water use efficiency. Furthermore, water reached deeper horizons under mulching than under conventional practice, resulting in 15% more deep percolation in a wet year. The simulation results also indicated that growing green manure during the fallow period decreased soil water storage, leading to lower wheat yields. Mulching proved to be an efficient measure for increasing yields, and possibly contributed to groundwater recharge.  相似文献   

Usefulness of near-infrared spectroscopy to determine biological and chemical soil properties: Importance of sample pre-treatment   总被引：3，自引：0，他引：3  

Thomas Terhoeven-Urselmans  Harald Schmidt  Rainer Georg Joergensen  Bernard Ludwig 《Soil biology & biochemistry》2008,40(5):1178-1188

Near-infrared reflectance spectroscopy (NIRS) is known for its inexpensiveness, rapidity and accuracy and may become a useful tool for the assessment of soil quality. Objectives were (i) to evaluate the ability of NIRS to predict several chemical and biological properties of organically managed arable soils as well as the properties of grain yield from winter cereals for a closed population and (ii) to test whether the use of field-moist and pre-treated (quick-freezing followed by freeze-drying and grinding) samples will generate similar results. One hundred and sixteen soil samples from nine organically managed farms from Germany sampled in 2005 and 2006 were used for this investigation. Spectra of the near-infrared region (including the visible range, 400–2500 nm) from field-moist (<2 mm) or pre-treated soil samples were recorded. A modified partial least-square regression method and cross-validation were used to develop an equation over the whole spectrum (first–third derivation). For the pre-treated soils, good predictions were obtained for pH, contents of organic C, total N, plant-available P (Olsen) and exchangeable K (calcium-acetate-lactate (CAL)), contents of microbial biomass C and N (C_mic and N_mic) and ergosterol, basal respiration, metabolic quotient, the ratio of organic C/total N, the grain yield of winter cereals and grain nitrogen uptake. The RSC (the ratio of standard deviation of laboratory results to standard error of cross-validation) was greater than 2.0, the correlation coefficients (r) of a linear regression (measured against predicted values) were greater than or equal to 0.9 and the regression coefficients (a) ranged from 0.9 to 1.1. Similar good predictions were obtained if field-moist samples were used, with the exception of P (Olsen), K (CAL), metabolic quotient, grain yield of winter cereals and grain nitrogen uptake (satisfactory predictions) and ergosterol content (unsatisfactory prediction). Good predictions of the contents of Mg (CaCl₂) and microbial biomass P (P_mic) were achieved for field-moist but not for pre-treated samples. Despite sample preparation, only satisfactory predictions were obtained for the ratios of C_mic/N_mic and ergosterol/C_mic and grain nitrogen content (1.4RSC2.0, r0.8 and 0.8a1.2). However, unsatisfactory predictions for field-moist and pre-treated samples were achieved for the content of P (CAL), the nitrogen mineralisation rate and the ratios of C_mic/P_mic and basal respiration/nitrogen mineralisation rate. Our results demonstrate that biological soil properties can be predicted with NIRS for closed populations in both sample states. The pre-treatment should be used if samples have to be stored prior to infrared measurements for periods longer than a month.  相似文献   

Hydrological pathway and source area of nutrient losses identified by a multi-scale monitoring in an agricultural catchment   总被引：6，自引：0，他引：6  

Jia-Liang Tang  Bin Zhang  Chao Gao  Harald Zepp 《CATENA》2008,72(3):374-385

The objectives of the study were to identify principal hydrological pathways and source areas of N and P losses by multi-scale monitoring and to estimate total nutrient losses from the catchment. An agricultural catchment with rain-fed agriculture and irrigated paddy fields in subtropical China was monitored with regularly sampling, together with intensive sampling during and after rain storms. Regular weekly sampling showed that the N concentrations in the overland flows from the upland and paddy fields were higher than those from the streams, but lower than those in the subsurface waters. The N concentration, on average, was 10.0 mg L^− 1 in the well and 1.7 mg L^− 1 in the spring water, the former was 10.2 times as high as that in the stream waters (1.0–1.5 mg L^− 1). Nitrogen and P in the overland flows originated dominantly in particulate forms from the uplands (over 70%) and in dissolved forms from the paddy fields. Inorganic N and P dominated in the streams and subsurface waters. The intensive sampling allowed us to establish flow-nutrient concentration relationships and to extrapolate nutrient losses during rainstorms without regular sampling. The extrapolation increased the estimated nutrient losses by about 30% to 50%. The average total nutrient losses within three water years were estimated as 21 kg N ha^− 1 yr^− 1 and 1 kg P ha^− 1 yr^− 1, accounting for 9.5% and 1.4% of chemical N and P fertilizers applied to the catchment after subtracting the nutrient inputs with irrigation and rainfall. The estimation showed that paddy fields were as important as the uplands in terms of nutrient losses. These results suggest that control of soil erosion and excessive irrigation could be effective to reduce nutrient export through overland flow and subsurface flow.  相似文献   

Browsing regime and growth response of Abies alba saplings planted along light gradients     

Andrea D. Kupferschmid  Ulrich Wasem  Harald Bugmann 《European Journal of Forest Research》2015,134(1):75-87

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Past,current and future drivers for the development of decision support systems in forest management     

Harald Vacik  Manfred J. Lexer 《Scandinavian Journal of Forest Research》2014,29(7):2-19

The potential for development in decision support for forest management is set by decision theory, available technology and methods. Demands for decision support are emerging from contemporary challenges and problems of forest management which act as stimuli for the science community. Objectives and approaches in forest management as well as technologies have been changing throughout history. Accordingly, the demand for tools to support planning and decision-making has evolved. In this contribution, the authors review the historic development of decision support systems (DSS) for forest management and discuss past, current and future drivers. Based on evidence from scientific literature, case studies in the frame of the Forest Management Decision Support Systems (FORSYS) action, as well as experiences of the authors some hypotheses about the future of DSS are drawn. It is shown that in the past, the drivers evolving from forest management as well as decision support technologies have influenced the way of how models and methods have been applied as well as how DSS architectures have been designed. It is concluded that in the future, the challenges for DSS development will increase, as the complexity of decision-making processes and the related models will compete with the user demands which ask for simplicity.  相似文献   

A vestige of Earth's oldest ophiolite   总被引：3，自引：0，他引：3  

Furnes H  de Wit M  Staudigel H  Rosing M  Muehlenbachs K 《Science (New York, N.Y.)》2007,315(5819):1704-1707

A sheeted-dike complex within the approximately 3.8-billion-year-old Isua supracrustal belt (ISB) in southwest Greenland provides the oldest evidence of oceanic crustal accretion by spreading. The geochemistry of the dikes and associated pillow lavas demonstrates an intraoceanic island arc and mid-ocean ridge-like setting, and their oxygen isotopes suggest a hydrothermal ocean-floor-type metamorphism. The pillows and dikes are associated with gabbroic and ultramafic rocks that together make up an ophiolitic association: the Paleoarchean Isua ophiolite complex. These sheeted dikes offer evidence for remnants of oceanic crust formed by sea-floor spreading of the earliest intact rocks on Earth.  相似文献   

Linear alkane polymerization on a gold surface     

Zhong D  Franke JH  Podiyanachari SK  Blömker T  Zhang H  Kehr G  Erker G  Fuchs H  Chi L 《Science (New York, N.Y.)》2011,334(6053):213-216

In contrast to the many methods of selectively coupling olefins, few protocols catenate saturated hydrocarbons in a predictable manner. We report here the highly selective carbon-hydrogen (C-H) activation and subsequent dehydrogenative C-C coupling reaction of long-chain (>C(20)) linear alkanes on an anisotropic gold(110) surface, which undergoes an appropriate reconstruction by adsorption of the molecules and subsequent mild annealing, resulting in nanometer-sized channels (1.22 nanometers in width). Owing to the orientational constraint of the reactant molecules in these one-dimensional channels, the reaction takes place exclusively at specific sites (terminal CH(3) or penultimate CH(2) groups) in the chains at intermediate temperatures (420 to 470 kelvin) and selects for aliphatic over aromatic C-H activation.  相似文献   

Long-range interactions within a nonnative protein     

Klein-Seetharaman J  Oikawa M  Grimshaw SB  Wirmer J  Duchardt E  Ueda T  Imoto T  Smith LJ  Dobson CM  Schwalbe H 《Science (New York, N.Y.)》2002,295(5560):1719-1722

Protein folding and unfolding are coupled to a range of biological phenomena, from the regulation of cellular activity to the onset of neurodegenerative diseases. Defining the nature of the conformations sampled in nonnative proteins is crucial for understanding the origins of such phenomena. We have used a combination of nuclear magnetic resonance (NMR) spectroscopy and site-directed mutagenesis to study unfolded states of the protein lysozyme. Extensive clusters of hydrophobic structure exist within the wild-type protein even under strongly denaturing conditions. These clusters involve distinct regions of the sequence but are all disrupted by a single point mutation that replaced residue Trp62 with Gly located at the interface of the two major structural domains in the native state. Thus, nativelike structure in the denatured protein is stabilized by the involvement of Trp62 in nonnative and long-range interactions.  相似文献