Pathogenesis of canine interdigital palmar and plantar comedones and follicular cysts, and their response to laser surgery     

Duclos DD  Hargis AM  Hanley PW 《Veterinary dermatology》2008,19(3):134-141

This study documents the presence of comedones and follicular cysts of palmar and plantar interdigital skin as an underlying cause of recurrent dermatitis, and describes the use of a carbon dioxide laser to surgically remove lesions. The 28 dogs included in the study had: (i) recurrent lameness, pain, and nodules, or draining sinuses in the dorsal interdigital skin, (ii) failed to respond to antibiotic therapy, and (iii) were negative for Demodex mites and dermatophytes. All 28 had laser surgery; nine dogs had two surgical procedures and two dogs had three surgical procedures for lesion recurrence. Fifteen dogs had skin samples collected for histopathology. Clinical features in ventral interdigital skin included alopecia, callus-like thickening, and comedones. Histological features included hyperkeratosis, acanthosis, comedones and follicular cysts, furunculosis, draining sinuses, and scarring. Surface trauma to the ventral interdigital skin appeared to contribute to lesion development. Laser surgery allowed removal of multiple layers of cysts and adjacent hair follicles and the tracking and removal of sinuses. One dog was euthanized for orthopaedic lameness 1 month after laser surgery, but post-surgical follow-up (1.0-8.0 years - mean 3 years) from the remaining 27 dogs revealed that laser therapy of affected skin and adjacent hair follicles resulted in resolution of interdigital lesions in 25. Two dogs continued to develop interdigital cysts.  相似文献   

追踪沙门菌病暴发的起源     

Billy M.Hargis  Renee M.Tsolis  徐仕忠 《中国家禽》2008,30(21)

在1980年代,欧洲和美洲的卫生官员们就注意到由肠炎沙门菌（SE）引起的人类食物源性疾病明显增加,发现这种沙门菌存在于鸡胴体、鸡蛋和蛋产品中。这提示,作为公共卫生问题,SE的出现可能是现代家禽养殖实践和家禽遗传多样性下降的结果。但是,这些假设没有说明为什么其它沙门菌血清型,如鼠伤寒沙门菌（ST）,感染人的数量没有增加。  相似文献   

Development of a rapid and inexpensive assay for the nonspecific detection of antimicrobial residues in chicken egg yolks and neonatal yolk sacs   总被引：1，自引：0，他引：1  

Caldwell DY  McReynolds JL  Young SD  Caldwell DJ  Hargis BM 《Journal of agricultural and food chemistry》2000,48(12):6431-6434

Competitive exclusion of intestinal pathogens by administration of beneficial and defined cultures of normal intestinal microflora is a safe and effective means of reducing the incidence and severity of chick infections with Salmonella and other intestinal pathogens. It is important that competitive exclusion cultures not carry genetic material (e.g., plasmids), which could transfer antibiotic resistance to other microflora, including pathogens. As such, safe and effective competitive exclusion cultures must be sensitive to commonly used antimicrobial agents. By necessity, intentional or accidental exposure of these beneficial microflora to antibiotics will reduce or eliminate the protection provided by competitive exclusion culture establishment. As antibiotic residues can be present from embryonic, hatchling, or maternal administration, a rapid and sensitive assay for the nonspecific detection of residues, which could interfere with competitive exclusion culture establishment, is needed. This study was conducted to develop a rapid and inexpensive bioassay to detect multiple antimicrobial residues in egg yolk and neonatal yolk sacs. Aerobic bacterial strains with known sensitivity to several antibiotics used by the poultry industry were selected and individually compared for sensitivity to enrofloxacin, gentamicin, tetracycline, ceftiofur, and tylosin concentrations in egg yolks. This assay was found to be relatively sensitive for the detection of these antimicrobials, and detection of residues was associated with reduced competitive exclusion culture (PREEMPT) establishment in one experiment. Importantly, this assay can be implemented with minimal training or equipment under commercial hatchery practices and could be used to determine embryo groups, in advance of hatch, that are not suitable candidates for competitive exclusion treatment in the hatchery.  相似文献   

Thyrocalcitonin: cytological localization by immunofluorescence     

G K Hargis  G A Williams  A Tenenhouse  C D Arnaud 《Science (New York, N.Y.)》1966,152(718):73-75

Thyrocalcitonin was detected in the cytoplasm of all epithelial cells of the thyroid gland of the pig, by means of antibody fluorescence. It was present in those cells whlich normally elaborate thyroglobulin but was not present in the follicular colloid.  相似文献   

Full-thickness cutaneous burn in black-haired skin on the dorsum of the body of a Dalmatian puppy     

Hargis  & Lewis 《Veterinary dermatology》1999,10(1):39-42

A 10-week-old Dalmatian puppy was presented for evaluation of plaques and eschars limited to the black-haired markings on the dorsum of the body. The plaques and eschars were of acute onset. Histopathologic evaluation revealed epidermal, adnexal, and vascular necrosis, and subepidermal vesiculation consistent with a full-thickness burn. Because of the history of exposure to direct sunlight, limitation of plaques and eschars to black-haired dorsal skin, histopathology, and lack of another identifying cause, the lesions were attributed to a thermal burn from sunlight.  相似文献