Diplococcus pneumonia Type 16 in Laboratory Rats          下载免费PDF全文

Harold Baer 《Canadian journal of veterinary research》1967,31(9):216-218

An acute respiratory disease in a colony of Wistar-derived rats is described. The infection, due to Diplococcus pneumoniae type 16, has apparently not been described previously in the rat. Rigid sanitary measures and treatment with penicillin were effective in controlling the disease.  相似文献   

Megimide, A New Barbiturate Antagonist          下载免费PDF全文

J. Harold Reed  James Archibald    L. M. Cobb 《Canadian journal of veterinary research》1957,21(5):165-169

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Differential leukocyte counts determined in chicken blood using the Cell-Dyn 3500     

Lilliehöök I  Wall H  Tauson R  Tvedten H 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(3):133-138

Background: Automated hematology instruments commonly are used for mammalian blood analysis, but there is a lack of accurate automated methods available for avian leukocyte analysis. Objective: The aim of this study was to validate differential leukocyte counts in blood from chickens using the Cell-Dyn 3500 hematology system and avian-specific software.
Methods: Blood samples were collected in lithium-heparin tubes from 2 groups (n = 84 and n = 139) of laying hens. Manual 200-cell differential counts were done on routinely-stained blood smears, and manual total granulocyte counts (heterophils and eosinophils) were done using an eosinophil stain in a counting chamber. Automated differential counts were done using VET 2.3, a research and development version of avian-specific software for the Cell-Dyn 3500. Results were analyzed using Pearson's correlation and difference plots.
Results: Automated granulocyte counts from the Cell-Dyn were in good agreement with manual granulocyte counts ( r = 0.93 and 0.80 for the 2 study groups). No correlation was found between automated and manual lymphocyte counts. Correlation coefficients for monocyte counts were 0.70 and 0.43. Conclusion: Automated leukocyte results from the Cell-Dyn using VET 2.3 software were not fully accurate. Total granulocyte counts may be of clinical usefulness, but results obtained for other parameters were unreliable.  相似文献   

Immunoglobulin M-capture enzyme-linked immunosorbent assay testing of cerebrospinal fluid and serum from horses exposed to west nile virus by vaccination or natural infection     

Porter MB  Long M  Gosche DG  Schott HM  Hines MT  Rossano M  Sellon DC 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(6):866-870

The West Nile (WN) virus, present in the United States since 1999, is a cause of encephalomyelitis in birds, alligators, humans, and horses. No data exist regarding detection of anti-WN virus immunoglobins in equine cerebrospinal fluid (CSF). The aims of this study were to evaluate the blood-brain barrier (BBB) in WN virus-infected (WNE) horses, to compare diagnostic testing in serum and CSF, and to describe the immunoglobulin M (IgM) response in serum and CSF of vaccinated horses. CSF was collected from the lumbosacral (LS) space (n = 13) or the allanto-occipital (AO) space (n = 14) of WNE horses. The albumin quotient (AQ) and IgG index were calculated, and the IgM-capture-enzyme-linked immunosorbent assay (MAC-ELISA) was used to detect anti-WN virus IgM in serum and CSF. CSF collected from the LS site had a higher (P < .02) IgG index compared to the AO site (0.34 +/- 0.04 versus 0.22 +/- 0.04 [mean +/- SE], respectively). The mean AQ, irrespective of collection site, did not exceed reference values. There was 100% agreement between CSF and serum testing for IgM by MAC-ELISA testing. However, the positive to negative antigen ratios were higher (P < .001) in CSF (34.5) versus serum (8.5), indicating lower nonspecific reactivity in CSF samples. Horses vaccinated against WN virus did not develop an IgM response at 1:400 mg/dL in serum; however, a few horses developed a weak IgM response in serum but not in CSF. In conclusion, MAC-ELISA testing of serum and CSF were equivocal. Also, examination of CSF data from WNE horses suggests a normal BBB integrity and increased intrathecal production of antibodies.  相似文献   

IMAGING DIAGNOSIS—OCCIPITOATLANTOAXIAL MALFORMATION IN A MINIATURE HORSE FOAL     

Diana S.  Rosenstein  DVM  MS  Harold C.  Schott II  DVMP hD  Russ L.  Stickle  DVM 《Veterinary radiology & ultrasound》2000,41(3):218-219

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采收和调制方法对晾制白肋烟中一些重要物质的影响   总被引：5，自引：0，他引：5  

杨焕文  崔明午 《云南农业大学学报(自然科学版)》1997,12(4):257-262

 通过6种不同的采收和调制方法对烟草特有的N-亚硝胺(TSNA)、烟草生物碱、硝酸盐、亚硝酸盐的影响,研究得出与对照整株采收晾制相比,逐叶采收、整株采收叶片划主脉、逐叶采收划主脉、逐叶采收叶肉主脉分离晾制能显着降低叶肉及主脉中烟草特有的N-亚硝胺含量,亦能降低硝酸盐及亚硝酸盐含量。整株采收划主茎晾制不能降低烟草特有的N-亚硝胺及硝酸盐、亚硝酸盐。各处理都能显着降低主脉和叶肉中的去甲基烟碱,但对其它烟草生物碱没有影响。  相似文献   

Jupiter's nightside airglow and aurora     

Gladstone GR  Stern SA  Slater DC  Versteeg M  Davis MW  Retherford KD  Young LA  Steffl AJ  Throop H  Parker JW  Weaver HA  Cheng AF  Orton GS  Clarke JT  Nichols JD 《Science (New York, N.Y.)》2007,318(5848):229-231

Observations of Jupiter's nightside airglow (nightglow) and aurora obtained during the flyby of the New Horizons spacecraft show an unexpected lack of ultraviolet nightglow emissions, in contrast to the case during the Voyager flybys in 1979. The flux and average energy of precipitating electrons generally decrease with increasing local time across the nightside, consistent with a possible source region along the dusk flank of Jupiter's magnetosphere. Visible emissions associated with the interaction of Jupiter and its satellite Io extend to a surprisingly high altitude, indicating localized low-energy electron precipitation. These results indicate that the interaction between Jupiter's upper atmosphere and near-space environment is variable and poorly understood; extensive observations of the day side are no guide to what goes on at night.  相似文献   

Cell polarity: endogenous ion currents precede and predict branching in the water mold achyla     

Kropf DL  Lupa MD  Caldwell JH  Harold FM 《Science (New York, N.Y.)》1983,220(4604):1385-1387

The hyphae of the water mold Achyla bisexualis generate electrical currents that enter the growing tips and leave farther back. An inward-moving current also precedes branching and predicts the site of branch emergence; during the branching process, the current at the original tip declines or even reverses transiently without any change in growth rate. The inward current probably acts as an early signal during branch differentiation. The flow of specific ions rather than the flow of electrical charge probably serves to localize growth.  相似文献   

TGF-beta signaling in fibroblasts modulates the oncogenic potential of adjacent epithelia     

Bhowmick NA  Chytil A  Plieth D  Gorska AE  Dumont N  Shappell S  Washington MK  Neilson EG  Moses HL 《Science (New York, N.Y.)》2004,303(5659):848-851

Stromal cells can have a significant impact on the carcinogenic process in adjacent epithelia. The role of transforming growth factor-beta (TGF-beta) signaling in such epithelial-mesenchymal interactions was determined by conditional inactivation of the TGF-beta type II receptor gene in mouse fibroblasts (Tgfbr2fspKO). The loss of TGF-beta responsiveness in fibroblasts resulted in intraepithelial neoplasia in prostate and invasive squamous cell carcinoma of the forestomach, both associated with an increased abundance of stromal cells. Activation of paracrine hepatocyte growth factor (HGF) signaling was identified as one possible mechanism for stimulation of epithelial proliferation. Thus, TGF-beta signaling in fibroblasts modulates the growth and oncogenic potential of adjacent epithelia in selected tissues.  相似文献   

A semi-pilot-scale procedure for isolating and purifying soybean (Glycine max) lectin     

Fasina YO  Swaisgood HE  Garlich JD  Classen HL 《Journal of agricultural and food chemistry》2003,51(16):4532-4538

Availability of gram quantities of purified soybean lectin (SBL) to scientists will foster discovery of novel biomedical applications of the lectin and provide the opportunity to investigate the antinutritional effects of SBL in soybean-consuming food animals and poultry. Therefore, a semi-pilot-scale procedure for isolating and purifying SBL was designed. Defatted soyflour was extracted overnight with 0.9% NaCl at 4 degrees C. The extract obtained was filtered (0.45 microm membrane) and subjected to affinity chromatography using a column containing N-acetyl-D-galactosamine resin that is specific for SBL. Bound SBL was eluted off the column with 0.14 M galactose solution. The eluent was ultrafiltered (30 kDa), and the resulting solution (SBL and water) was freeze-dried. Electrophoretic analysis and hemagglutination assay revealed that the freeze-dried SBL was similar to Sigma-grade SBL in purity and activity (35 and 33 HU/mg protein, respectively). The procedure yielded 141 mg of SBL/100 g of soyflour.  相似文献