Effects of yield-related QTLs SPIKE and GPS in two indica rice genetic backgrounds     

Toshiyuki Takai  Shunsuke Adachi  Daisuke Fujita  Yumiko Arai-Sanoh  Masaki Okamura  Motohiko Kondo 《Plant Production Science》2017,20(4):467-476

Recent progress in rice genomics has promoted the identification of quantitative trait loci (QTLs) associated with yield and its related traits. SPIKE, a QTL controlling spikelet number per panicle, and GPS, a QTL controlling leaf photosynthesis rate, were identical to NAL1. To assess the effect of SPIKE/GPS on yield potential, we compared DNA sequences of these alleles and conducted yield experiments in the field of Japan using the near-isogenic lines NIL-SPIKE (allele from Daringan in IR 64 genetic background), NIL-GPS (allele from Koshihikari in Takanari genetic background), and a chromosome segment substitution line, SL2115 (allele from Koshihikari in IR 64 genetic background). Despite the two SNPs in the promoter regions between Koshihikari and Daringan, both alleles were effective to increase the number of spikelets per panicle both in Takanari and IR 64 backgrounds. However, the extent of the increase was smaller and unstable in Takanari background than that in IR 64 background. In addition, SPIKE/GPS improved percentage of filled spikelets only in the IR 64 background. These results suggest that the effects of SPIKE/GPS alleles are similar but are affected by the difference of the genetic backgrounds. Because the increasing effect of spikelets number per panicle was canceled by the decrease of the number of panicles, which seems to be affected by environmental factors, none of NIL-SPIKE, SL2115, or NIL-GPS significantly out-yielded their parental cultivars. These results indicate the importance to consider genetic backgrounds and QTL-environment interaction toward the future use of SPIKE/GPS.  相似文献   

In vivo and in vitro studies using larval and adult antigens from Neobenedenia melleni on immune response in yellowtail (Seriola lalandi)          下载免费PDF全文

M Reyes‐Becerril  E Alamillo  A Trasviña  I Hirono  H Kondo  W Jirapongpairoj  F Ascencio‐Valle  C Angulo 《Journal of fish diseases》2017,40(11):1497-1509

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Expression and regulation of estrogen receptor 2 and its coregulators in mouse granulosa cells     

Chihiro EMORI  Takuya KANKE  Haruka ITO  Yuki AKIMOTO  Wataru FUJII  Kunihiko NAITO  Koji SUGIURA 《The Journal of reproduction and development》2022,68(2):137

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A long-term observation for ecology of pathogenic Yersinia in wild rodents living in Fukushima Prefecture,Japan     

Thi Hien BUI  Khanh Thuan NGUYEN  Shunsuke IKEUCHI  Haruka YANAGAWA  Yomogi SATO  Thi Hong To TRAN  Minato OKUMURA  Takeshi NIWA  Takahide TANIGUCHI  Hideki HAYASHIDANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1790

From 2012 to 2021, prevalence of pathogenic Yersinia in wild rodents captured in Fukushima Prefecture, Japan was investigated twice a year to clarify the ecology of this pathogen in wild rodent populations. Pathogenic Yersinia enterocolitica O8 was isolated from 13 (1.7%) of 755 wild rodents. The Y. enterocolitica O8 isolates harbored three virulent genes (ail, fyuA, and virF). This pathogen was isolated repeatedly from wild rodents in April 2015, 2016, and 2017, in June and November 2020, and in April 2021, which was 6 of 19 times of observations. All Y. enterocolitica O8 isolates showed the same PFGE patterns. These results indicated that the same clone of pathogenic Y. enterocolitica O8 has been maintained in wild rodent populations in Fukushima Prefecture. Therefore, wild rodent populations contribute substantially to the continuous transmission of Y. enterocolitica O8 and its persistence in the ecosystem. This is the first report on the isolation of pathogenic Y. enterocolitica O8 in wild rodents in Fukushima Prefecture, Japan.  相似文献   

Possible involvement of cyclic AMP as an inhibitory regulator in thrombocyte aggregation induced by collagen in carp <Emphasis Type="Italic">Cyprinus carpio</Emphasis>     

Teruo Matsushita  Shin -Ichi Inoue  Ryusuke Tanaka  Masakazu Kondo  Yukinori Takahashi  Mitsuko Miyamoto  Hajimu Kurumaiani  Toshio Nakaki 《Fisheries Science》2008,74(1):214-216

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Drug resistance mechanism of the fish-pathogenic bacterium Lactococcus garvieae     

Maki T  Hirono I  Kondo H  Aoki T 《Journal of fish diseases》2008,31(6):461-468

The minimum inhibitory concentrations (MICs) of 15 chemotherapeutic agents were tested against 146 Lactococcus garvieae strains isolated from 1999 to 2006 in Japan. The agents used included chloramphenicol, ciprofloxacin, erythromycin (EM), enoxacin, fleroxacin, florfenicol, kanamycin, lincomycin (LCM), norfloxacin, oxolinic acid, orbifloxacin, ofloxacin, benzylpenicillin, streptomycin and tetracycline (TC). Of the tested strains, 46 showed high levels of resistance to EM, LCM and TC. Twelve of these strains were detected to be carrying transferable R-plasmids using a conjugation experiment and, using Southern hybridization, were shown to have the same structure as the R-plasmid. The remaining 34 resistant strains had a similar DNA structure to that of the R-plasmid as confirmed by polymerase chain reaction (PCR) using primers designed from sites in the transferable R-plasmid. The EM and TC resistance genes were classified into the ermB and tetS groups using PCR. We also detected gyrA and/or parC mutants that are highly resistant to old and new generation quinolones. This study revealed that transferable R-plasmids encoding EM, LCM and TC are widely distributed and are conserved regardless of the area and/or time of collection.  相似文献   

Cloning of ATP-dependent protease ClpXP genes in Aeromonas veronii     

Han HJ  Kondo H  Vivekanandhan G  Hirono I  Aoki T 《Journal of fish diseases》2006,29(11):691-695

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Molecular characterization and expression analysis of heat shock proteins 40, 70 and 90 from kuruma shrimp <Emphasis Type="Italic">Marsupenaeus japonicus</Emphasis>     

Threechada Danwattananusorn  Fernand F. Fagutao  Aiko Shitara  Hidehiro Kondo  Takashi Aoki  Reiko Nozaki  Ikuo Hirono 《Fisheries Science》2011,77(6):929-937

Heat shock proteins (HSPs) are proteins that are expressed more strongly when the cells are exposed to physiological and stressful conditions. In this study, the full-length cDNAs of heat shock proteins 40 (MjHSP40), 70 (MjHSP70) and 90 (MjHSP90) were cloned from kuruma shrimp Marsupenaeus japonicus. The open reading frames (ORFs) of the cDNA clones have lengths of 1,191, 1,959 and 2,172 bp and encode 396, 652 and 723 amino acid residues, respectively. The predicted MjHSP40 amino acid sequence contains a J domain, a glycine/phenylalanine-rich region, and a central domain containing four repeats of a CxxCxGxG motif, indicating that it is a type I HSP40 homolog. The signature sequences of the HSP70 and HSP90 gene families are conserved in the MjHSP70 and MjHSP90 amino acid sequences. The deduced amino acid sequences of MjHSP70 and MjHSP90 share high identity with previously reported shrimp HSP70s and HSP90s, respectively. The expression of MjHSP90 mRNA increased at 32°C. Additionally, the expressions of MjHSP40, MjHSP70 and MjHSP90 mRNAs increased in defense-related tissues (i.e., hemocytes and lymphoid organ) when the shrimp were challenged with white spot syndrome virus.  相似文献