Efficacy of omeprazole paste in the prevention of gastric ulcers in 2 years old thoroughbreds     

Endo Y  Tsuchiya T  Sato F  Murase H  Omura T  Korosue K  Nambo Y  Ishimaru M  Wakui Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(8):1079-1081

Prevalence of equine gastric ulcer syndrome in 85 young Thoroughbreds was investigated. The presence of gastric ulcers was confirmed in 27.1% (23/85) of the horses by endoscopic examination. Sixty-two horses without gastric ulcers were allocated randomly to either the treated group (31 horses) or sham-dosed control group (31 horses) in order to investigate the efficacy of omeprazole oral paste in the prevention of gastric ulcers. At the second endoscopic examination conducted after 28 days of administration, only 1 horse in the treated group developed gastric ulcers, while 12 horses developed gastric ulcers in the control group. Based on these data, the efficacy of omeprazole in prevention of equine gastric ulcers in young Thoroughbreds during the training period was confirmed.  相似文献   

Association of the amino acid motifs of BoLA-DRB3 alleles with mastitis pathogens in Japanese Holstein cows     

Tatsuyuki YOSHIDA  Harutaka MUKOYAMA  Hiroki FURUTA  Yasuko KONDO  Shin-nosuke TAKESHIMA  Yoko AIDA  Motoaki KOSUGIYAMA  Hiroshi TOMOGANE 《Animal Science Journal》2009,80(5):510-519

The association of the polymorphism of bovine leukocyte antigen ( BoLA-DRB3 ) genes, identified by the polymerase chain reaction sequence-based typing (PCR-SBT) method, with resistance and susceptibility to mastitis caused by Streptococci , coagulase-negative Staphylococci, Escherichia coli and Staphylococcus aureus was investigated. Blood samples for DNA extraction were collected from 170 Holstein cows (129 mastitis and 41 healthy cows) from 5 districts in Chiba prefecture, Japan. Susceptibility or resistance to the mastitis-causing pathogens was thought to vary by the presence of amino acid substitutions at the 9, 11, 13, and 30 positions. DRB3*0101 and DRB3*1501 had amino acid motifs of Glu⁹, Ser¹¹, Ser¹³, and Tyr³⁰, and they were considered to have susceptibility to all 4 mastitis pathogens. In contrast, DRB3*1101 and DRB3*1401 had amino acid motifs of Gln⁹, His¹¹, Gly¹³, and His³⁰ in these positions, and they also had Val⁸⁶, so these alleles were considered to have resistance to Streptococcal and coagulase-negative Staphylococcal mastitis. However, in the case of Escherichia coli mastitis, amino acid substitutions at the 9, 11, 13, and 30 positions had little effect, but rather substitutions at the 47, 67 positions of pocket 7, and at the 71, 74 positions of pocket 4, Tyr⁴⁷, Ile⁶⁷, Ala⁷¹, and Ala⁷⁴, were associated with resistance. This motif was present in DRB3*1201 .  相似文献   

Case report: The case of a 17 kg ovarian granulosa cell tumor in a Breton draft mare     

Munkhtuul TSOGTGEREL  Masaaki TAGAMI  Kenichi WATANABE  Harutaka MURASE  Yuko HIROSAWA  Yoshiyasu KOBAYASHI  Yasuo NAMBO 《Journal of Equine Science》2021,32(2):67

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Seasonal changes in spermatogenesis and testicular steroidogenesis in wild male raccoon dogs (Nyctereutes procynoides)     

Qiang W  Murase T  Tsubota T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(10):1087-1092

Testes of 15 wild adult male raccoon dogs (Nyctereutes procynoides) obtained from September 2000 to April 2001 were studied to clarify seasonal changes in spermatogenesis and testicular steroidogenesis. There were marked seasonal variations in the testis weight and size with values relatively low in September and highest in March. Spermatogonia and primary spermatocytes were observed in September, while spermatogonia, spermatocytes and round spermatids were present in January, and all types of spermatogenic cells including mature spermatozoa were found in the mating season (February and March). The number of spermatogenic cells reached their peak values in February and March. In addition, steroidogenic enzymes were immunolocalized using polyclonal antisera raised against bovine adrenal cholesterol side-chain cleavage cytochrome P450 (P450scc), human placental 3beta-hydroxysteroid dehydrogenase (3 betaHSD), porcine testicular 17alpha-hydroxylase cytochrome P450 (P450c17), and human placental aromatase cytochrome P450 (P450arom). P450scc and P450c17 were identified in Leydig cells and spermatids in February, whereas these enzymes were present only in Leydig cells in September. 3betaHSD was found in Leydig cells in September and February with more intense staining in February. The localization of P450arom changed seasonally: no immunostaining in September; more extensive immunostaining in Leydig cells, Sertoli cells, and elongating spermatids in February. These results suggest that seasonal changes in the testis weight and size of wild male raccoon dogs are correlated with changes in spermatogenesis. Seasonal changes in testicular steroidogenesis suggest that the synthesis of androgen and estrogen reaches its peak in the mating season.  相似文献   

Sex identification of Japanese black bear,Ursus thibetanus japonicus,by PCR based on amelogenin gene     

Yamamoto K  Tsubota T  Komatsu T  Katayama A  Murase T  Kita I  Kudo T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):505-508

A method for sex identification of the Japanese black bear was examined using a polymerase chain reaction (PCR) and sequencing of a part of the amelogenin gene. This gene is located on the X and Y chromosomes, and there are 54 nucleotide deletions on the Y chromosome-specific gene. Forty-seven (26 male and 21 female) DNA samples and 23 (13 male and 10 female) DNA samples, respectively extracted from white blood cells and hairs of Japanese black bears were analyzed. The primers SE47 and SE48 from this X-Y homologous region were used in sex identification by PCR amplification. These primers amplified X- and Y-specific bands, which could be used to discriminate between sexes by a length polymorphism in all samples. We suggest that PCR amplification using the primers SE47 and SE48 is useful for sex determination of the Japanese black bear and could be applied to DNA analysis of small samples such as hairs.  相似文献   

FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex   总被引：5，自引：0，他引：5  

Abe M  Kobayashi Y  Yamamoto S  Daimon Y  Yamaguchi A  Ikeda Y  Ichinoki H  Notaguchi M  Goto K  Araki T 《Science (New York, N.Y.)》2005,309(5737):1052-1056

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Anaerobic reoxidation of Mn2+, Fe2+, S0 and S2– in submerged paddy soils     

J. Murase  M. Kimura 《Biology and Fertility of Soils》1997,25(3):302-306

Anaerobic reoxidation of reduced products in paddy soils was investigated. Ferrous iron (Fe²⁺) and monosulfide ion (S^2–) added to the soil chemically reduced MnO₂ to Mn²⁺, and MnO₂ and Fe(OH)₃ to Mn²⁺ and Fe²⁺, respectively, where Fe²⁺ and S^2– were considered to be oxidized to Fe³⁺ and S⁰. Elemental sulfur was oxidized to sulfate by anaerobic incubation with NO₃ ^– MnO₂ and Fe(OH)₃. A new conceptual model for the reduction processes in submerged paddy soil including the reoxidation processes of reduced products, in which soil heterogeneity in paddy fields was taken into consideration, was proposed based on the results. Received: 20 October 1996  相似文献   

Eating until feeling full and rapid eating both increase metabolic risk factors in Japanese men and women     

Hsieh SD  Muto T  Murase T  Tsuji H  Arase Y 《Public health nutrition》2011,14(7):1266-1269

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Population Statistics and Biological Traits of Endangered Kiso Horse     

Masaki TAKASU  Nana HIRAMATSU  Teruaki TOZAKI  Hironaga KAKOI  Telhisa HASEGAWA  Masami MAEDA  Huricha  Satoshi KUSUDA  Osamu DOI  Tetsuma MURASE  Harutaka MUKOYAMA 《Journal of Equine Science》2011,22(4):67-72

The objective of this study was to clarify the current status of endangered Kiso horse, population statistics and biological traits, in order to take a step for the conservation by scientific approach. We surveyed 125 Kiso horses (86.2% of the whole breed), analyzed the construction of the population, and calculated the coefficient of inbreeding and effective population size. Moreover, we confirmed coat color variations and the traditional traits of the Kiso horse, and measured their height at the withers and chest circumference to clarify their physical characteristics. The population pyramid of the horses was stationary or contractive, suggesting a reduction of the population in the near future. The effective population size of the horse (47.9) suggested that the diversity was much less than their census size, and the high coefficient of inbreeding, 0.11 ± 0.07 on average, suggested that the horses were surely inbred. The horses had only 4 coat colors; bay, dark bay, buckskin dun, and chestnut, and 116 horses (92.8%) were bayish color, suggesting the fixation in their coat color. Moreover, the majority of them had dorsal stripe (83 horses; 66.4%), and the average heights at withers(131.9 ± 4.4 cm) and chest circumference (167.1 ± 10.1 cm) were not significantly different between males and females.  相似文献   

Effect of probability-distance based Markovian texture extraction on discrimination in biological imaging     

S.N. Ondimu  H. Murase 《Computers and Electronics in Agriculture》2008,63(1):2-robotics

The effect of probability-distance based re-weighting of image texture features on their discrimination ability was evaluated in this study. A quadratic surface smoothing function was developed from the Bhattacharyya probability distance between two classes of images. The function was used as a re-weighting function for standard grey level co-occurrence matrix (GLCM) textural features in two cases. Case 1 involved 42 images of health and smokers’ lungs. Case 2 involved 144 images of well watered; partially water stressed and dry Rhacomitrium canescens plants. Multilayer perception (MLP) neural networks (NN) classifiers based on standard weighted and probabilistic-distance based re-weighted GLCM textural features were developed for each case. The classifiers were trained and tested using the leave-one-out and the cross-validation evaluation strategies.Probability-distance based re-weighting of GLCM textural features resulted in 37% and 39.22% reduction in true classification error for cases 1 and 2, respectively. It was concluded that re-weighting image texture features based on the probability distance between the classes involved, makes the GLCM texture analysis technique more discriminative and adaptive. This modification will help to overcome the fixed nature of GLCM features which is an area of weakness open to further improvement. Although GLCM features were used in this study, the method can be extended to other co-occurrence-based methods.  相似文献