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601.
1. An experiment was carried out to evaluate the effect of early feed restriction (FR) on immunocompetence of Ross and Arian chickens with separated sexes under heat stress (HS) conditions. 2. Chickens consumed feed ad libitum (AL) or were restricted on alternate days from 11 to 20 d of age. From 35 to 41 d of age, the HS groups were exposed to a high ambient temperature of 39 +/- 1 degreesC for 7 h each day, while the thermoneutral groups (TN) were at 33 degrees C. 3. At 21 and 42d of age, the percentage of CD4+ (helper T cells) and CD8+ (cytotoxic T cells) were determined by flow cytometry. Antibody response to sheep red blood cells (SRBC) and heterophil/lymphocyte (H/L) ratio were determined on d 21 and 42. 4. On d 21, FR elevated the CD4+, antibody titre and H/L ratio, but it decreased the CD8+ T cells. On d 42, HS decreased CD4+, CD8+, and antibody titre, but it increased H/L ratio. Under TN conditions, FR chickens had higher CD4+ than AL chickens. On d 42, FR/HS chickens had higher CD4+ and antibody titre, but they had lower CD8+ and H/L ratio than AL/HS chickens. 5. On d 42, the TN-Ross strain had lower CD4+, but they had higher CD8+ and antibody titres than the TN-Arian strain. On d 42, the HS-Arian strain had higher antibody titres and a lower H/L ratio than the HS-Ross strain. 6. Male chickens had higher CD4+, CD8+, antibody titres and H/L ratios 25 in all treatment groups. 7. In conclusion, FR early in life reduced some of the negative effects of the heat stress on the immune system of broiler chickens when exposed to high environmental temperatures later in life.  相似文献   
602.
In the present study three phenotypically CAMP-negative Streptococcus agalactiae, isolated from three cows with mastitis, were characterized by molecular analysis. An identification of the S. agalactiae was performed by conventional methods and by PCR amplification of species specific parts of the 16S rRNA gene and the 16S-23S rDNA intergenic spacer region. In addition all three phenotypically CAMP-negative isolates harboured a normal sized CAMP-factor encoding cfb gene indicating a reduced expression of CAMP-factor or a gene defect elsewhere along the pathway of expression. The clonal identity of the three isolates could be demonstrated by macrorestriction analysis of their chromosomal DNA.  相似文献   
603.
604.
A comparison of a monoculture of Nile tilapia Oreochromis niloticus and a polyculture of carps (silver carp Hypophthalmichthys molitrix; rohu Labeo rohita; and mrigal Cirrhinus mriga la; ratio 4:3:3) was carried out in 200-m2 earthen ponds fertilized with cattle manure and supplemented with inorganic fertilizer at 3-kg nitrogen and 1.5-kg phosphorus/ha per day. A control treatment of a tilapia monoculture without fertilizer inputs was included to assess the effect of pond basal fertility. Net yields of 23.5 kg/pond per 112 d (3.8 t/ha per 1 yr) in the tilapia monoculture and 19.2 kg/pond per 112 d (3.1 t/ha per yr) in the carp polyculture were not significantly different; net yields from unfertilized tilapia monoculture ponds were negative. In the carp polyculture, silver carp was the dominant species at harvest contributing 73% of the total net fish production compared to 9% and 19% by rohu and mrigal, respectively. Water quality data suggested that tilapia yields could have been further improved by increasing fertilization rate but that critical dissolved oxygen concentration constrained this option for carp polyculture.  相似文献   
605.
Experiments undertaken on Rusinga Island, Kenya, indicated that Rhipicephalus appendiculatus (Neumann) was the predominant tick consumed on tick-infested cattle by chickens, followed by Amblyomma variegatum (Fabricius), Boophilus decoloratus (Koch) and Rhipicephalus evertsi evertsi (Neumann). The number of ticks consumed by chickens released with cattle in a 3 h release period was not significantly different from ticks consumed in a 4 h release period. In a free management system, chickens 1-3 months of age consumed more ticks than older ones; in a confined management system however, chickens 4-6 months of age consumed more ticks than younger birds. Although the numbers of ticks consumed by local and exotic breeds of chickens were not significantly different, a period of 8-10 weeks was necessary for the exotic breeds to become behaviorally adapted to tick predation on cattle. Local chickens bred in environments where cattle were absent took a shorter period of 4-6 weeks to adapt. It was concluded that the relatively large number of ticks consumed by each chicken during the 3 or 4 h release period is an indication that a good reduction of ticks on cattle can be achieved through tick predation by chickens. The preponderance of R. appendiculatus over other tick species consumed was a reflection of its relative abundance on cattle on the island rather than a selective preference for tick species.  相似文献   
606.
Chickens were shown to be natural predators of ticks. Rhipicephalus appendiculatus were recovered in large numbers from the crops and gizzards of chickens which had scavenged for 30 min-1 h among tick-infested cattle. Other ticks recovered were Amblyomma variegatum and Boophilus decoloratus. The numbers of ticks recovered ranged from 3 to 331, with an average of 81 per chicken. Cattle facilitated the predation of ticks by certain behavioural actions. Chickens also picked up both engorged and unengorged ticks seeded on vegetation, but unengorged ticks were preferred. The possibility of tick predation by chickens forming a viable biological control component in an integrated tick management system in Africa is discussed.  相似文献   
607.
Oxfendazole was tested against Raillietina tetragona in experimentally infected chickens using single oral doses of 20, 10, 7.5, 5, and 2.5 mg/kg body weight. The minimal dose of the drug which produced 100% efficacy against immature worms was 10 mg/kg whereas the same effect on the mature parasite was obtained with 7.5 mg/kg. Doses lower than these significantly reduce worm burdens. The compound appears to be safe for chickens and a dose of 20 mg/kg (twice the recommended dose) produced no untoward clinical reactions.  相似文献   
608.
In the late 1970s the whitefly Bemisia tabaci (Gennadius) became the primary cotton pest in the Sudan, superseding the American bollworm Heliothis armigera (Hübner). DDT and a DDT/dimethoate combination were used to control the bollworm and, simultaneously, jassids and whiteflies. B. tabaci, a secondary pest at first, became resistant to dimethoate by frequent selections from 1964 onwards. At the same time, fertility stimulation occurred due to DDT residues on cotton plants. Finally, resistance reached a level so that the whitefly were not controlled by dimethoate, monocrotophos or other organophosphorus insecticides, and stimulation by DDT could exert its full effect. The consequence of this was a tremendous flare-up of the whitefly by 1980/81. This train of events was concluded from laboratory and field studies of the resistance patterns, as well as the acceleration effects from DDT residues on plants to the whitefly. A current hypothesis claiming that the problems arose from the elimination of beneficial insects through insecticide applications is reviewed in the light of experimental evidence and practical experience.  相似文献   
609.
The study was conducted to examine the chemical composition of urea–molasses treated wheat straw (WS) fermented with cattle manure (CM) and its feeding value for growing buffalo male calves. Wheat straw treated with varying levels of urea (0%, 2% and 4%) and molasses (2% and 4%) was ensiled with 30% cattle manure (on dry matter basis) for different fermentation periods (20, 30 and 40 days). Fermented wheat straw (FWS) after each fermentation period was analyzed for pH, dry matter (DM), crude protein (CP), true protein (TP), ammonia nitrogen (NH3-N), acid detergent fiber (ADF) and neutral detergent fiber (NDF). Maximum pH, DM, CP, TP and NH3-N and minimum NDF contents were observed with 4% urea. The 4% molasses level increased the CP, TP and NH3-N contents of FWS, but pH, DM and NDF remained unchanged after 40 days of fermentation. The 4% urea × 4% molasses interaction resulted in maximum DM, CP, TP, NH3-N after 40 days of fermentation period and this combination was used for large scale production of FWS to evaluate its feeding value for calves. Four isonitrogen and isocaloric diets were formulated. The control FWS 0 diet contained no FWS while in FWS 15, FWS 25 and FWS 35 diets concentrate was replaced with 15%, 25% and 35% FWS, respectively. The diets were randomly allotted to four groups of 28 calves 9–12 months of age, seven in each group, in a randomized complete block design to examine the nutrient intake, digestibility, nitrogen balance and weight gain. An increased DM, organic matter (OM), CP and NDF intakes were observed in calves fed FWS diets compared to those fed FWS 0 diet. Nitrogen retention increased significantly with increasing the level of FWS. The DM, OM, CP and NDF apparent digestibilities were non-significant in calves fed different levels of FWS. A linear increase in weight gain was noticed in calves fed diets containing increasing level of FWS.  相似文献   
610.
Knowledge of the relationship between dairy herd management and milking practices with the occurrence of Listeria monocytogenes on dairy farms might assist in the development of intervention strategies aimed at eliminating this organism at the pre-harvest dairy-food-production level. This paper represents a first step towards that goal. We carried out a cross-sectional study to identify farm factors that were associated with isolation of L. monocytogenes from on-farm in-line milk-filters. Data on these factors were collected by personal interview of the farm owners or managers.Logistic regression was used to evaluate the significance of association of each factor while simultaneously controlling for the presence of other factors. A systematic approach was developed in which the bivariable association of the hypothesized factors initially was evaluated. All significant factors were then jointly evaluated in a multivariable logistic model.Farms that used a bucket system had significantly higher odds of L. monocytogenes as compared to farms that used a round-the-barn pipeline milking system (OR=0.35,P=0.05) or milking parlor (OR=0.21,P=0.01). There was a significant association between pre-milking teat disinfection (OR=0.26,P=0.001) and pre-milking examination of abnormal appearance of milk (OR=0.4,P=0.01) against the occurrence of L. monocytogenes. We also found a significant association between the use of E. coli J5 vaccine (OR=3.3,P=0.03) and how long dry-cow therapy had been used on farm (OR=0.34,P=0.04).  相似文献   
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