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91.
Histophilus ovis was shown to be phagocytized by neutrophils when the organisms enter the lumen of the reproductive tract of the ram. The phagocytosis and destruction of H. ovis by neutrophils was demonstrated in vitro by the viable count method and by electron microscopy. It was shown that immunoglobulins and complement had no influence on the phagocytosis and destruction of H. ovis. Phagocytosis and killing of H. ovis was accomplished equally well by neutrophils from immunized and non-immunized rams. Immunized rams showed a massive infiltration of neutrophils into the walls, epithelium and lumen of their ampullae when dead H. ovis were introduced into their lumen.  相似文献   
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Unsolicited reports of estrous behavior in mares thought to be pregnant were received from owners or caretakers of Arabian mares. Estrous behavior was confirmed and mares were examined for pregnancy. Gender of the conceptus was determined at foaling in 11 mares in which estrous behavior was confirmed while an apparently viable, ultrasonically normal-appearing conceptus was present. In 9 mares in which the day of ovulation was known (Day 0), the estrous behavior occurred on Day 12, 13 or 14 (5 mares), Day 18 or 20 (2 mares), Day 40 (1 mare) and Day 60 (1 mare). In another study, 55 pony mares were observed for estrous behavior every 3 days for 20 minutes during Days 11 to 40. Estrous behavior was observed in 1 mare (2%) on Day 24. Combined for the 2 studies, the incidence of a female conceptus (12/12) was greater (P<0.01) than the incidence of a male conceptus (0/12) in mares that exhibited estrous behavior.  相似文献   
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Between January 1972 and December 1990, bulk-tank (n = 721) and cow (n = 9,163) milk samples from dairy herds in New York State were examined by bacteriologic procedures for Mycoplasma. The organism was found in 165 herds in 42 counties, and in 2.3 and 11.7% of the tank and cow samples, respectively. Mycoplasma bovis was isolated in 164 herds, M. californicum was isolated in 1. Highest incidence of mycoplasmal clinical mastitis occurred during the winter. The disease resulted in culling of 30-70% of the cows in several herds. Eighty-six of the positive herds were located in the western part of the state. This area had more large herds (greater than 200 cows) compared to the rest of the state; however, herd size was not a risk factor. Purchased animals added to herds without quarantine, poor hygiene during mastitis treatment, and personnel in contact with mastitic cows or infected milk were involved in outbreaks and disease transmission.  相似文献   
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The effects of the sterol biosynthesis inhibitor (SBI) fungicides fenarimol, fenpropimorph, imazalil, prochloraz, propiconazole and triadimenol on growth and sterol composition of Ustilago maydis, Botrytis cinerea and Pyrenophora teres, grown from spores or sporidia in liquid culture, were determined. Growth of U. maydis was only slightly inhibited by SBI fungicides at concentrations which caused considerable changes in both sterol content and composition. Conversely, in B. cinerea and P. teres, growth was strongly inhibited under conditions where ergosterol was still the predominant sterol, suggesting that, in these two fungi, growth may be more sensitive to SBI fungicides than overall sterol production. Demethylase inhibitor fungicides behaved as a homogeneous group in their effects on growth and on sterol profiles of the three fungi studied.  相似文献   
99.
Uptake and translocation of 26 chemicals (herbicides, fungicides, growth regulators, insecticides and model compounds), formulated with and without ‘Ethylan TU’ (NP8) surfactant, have been determined 24, 48 and 72 h after spraying onto leaves of maize, rape, strawberry and sugar beet. Eight of the chemicals may be classified as polar (log partition coefficient (P) < 0) and 18 as lipophilic (log P 0·8-4·6). Chemicals penetrated more readily into waxy leaves of rape and strawberry than into less-waxy sugar beet, whilst lowest rates were observed for maize. Rates of uptake varied from exceedingly low (< 2% 72 h after application) for uracil, maleic hydrazide, isoproturon and methyl phenylurea applied to maize, to rapid (>98% within 24 h of application) for acephate and triadimefon applied to strawberry leaves. The effects of NP8 on rates of chemical uptake were classified into 4 types, viz:-
  • (i) low rates, with limited enhancement by NP8;
  • (ii) high rates unaffected by NP8;
  • (iiii) marked enhancement by NP8 within 24 h; and
  • (iv) continuous enhancement by NP8 over 0-72 h.
Translocation into the tissues immediately adjacent to the target site was greater for waxy than less-waxy plants, enhancement by NP8 was attributed to its effects on penetration rather than to surfactant interactions at the sub-cuticular level. Logarithms of physicochemical properties (partition coefficient, water solubility S, molecular weight MW), together with deposit area and melting point (m.p.) values were used to investigate relationships with uptake and translocation. In most cases the variability could not be described adequately in a simple linear form. Regressions using the entire group of 26 chemicals also showed poor correlations between uptake patterns and individual variables. Polynomials in log S, log P, log MW and m.p. were used to test for complex relationships. A quadratic polynomial in log P, log S and m.p. accounted for slightly more (77%) of the variability between the means for total uptake than a cubic polynomial in log P and log S (75%). Although both regressions were significant, the ‘lack of fit’ indicated that other factors need to be included to account fully for the variability in rates of uptake and translocation.  相似文献   
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