Lymphocyte blastogenic responses of calves experimentally infected with Ostertagia ostertagi     

D A Cross  P H Klesius  T B Haynes 《Veterinary parasitology》1986,22(1-2):49-55

Twelve 9-week-old calves were divided into four groups; two groups were maintained helminth-free as controls and the other groups were given Ostertagia ostertagi infective-stage larvae (L3) orally. One group received 100,000 L3 as a single inoculum and the other group received L3 in increasing dosages at weekly intervals for 8 consecutive weeks. The blastogenic responses to concanavalin A (Con A), phytohemagglutinin (PHA), and a soluble larval antigen from O. ostertagi (SLA) of peripheral blood lymphocytes were evaluated using tritiated thymidine incorporation into DNA as a measure of blastogenesis. The responses to Con A of all infected calves were significantly depressed while the responses to PHA were not. SLA, at concentrations of 4 micrograms ml-1 and above, caused blastogenic activity in lymphocytes from uninfected calves. Using SLA at 1 microgram ml-1 in lymphocyte cultures supplemented with autologous serum, an antigen-induced blastogenic response was detected in calves receiving serial inoculations of L3.  相似文献   

Immunomodulation with killed Propionibacterium acnes in guinea pigs simultaneously vaccinated with Brucella abortus strain 19     

V S Panangala  T B Haynes  R D Schultz  A Mitra 《Veterinary immunology and immunopathology》1986,13(1-2):71-84

Immunomodulation with killed Propionibacterium acnes was attempted in guinea pigs simultaneously vaccinated with Brucella abortus strain 19. Two groups, each comprised of 9 guinea pigs, were injected by different routes (s.c. and or i.v.) with 1.4 mg of P. acnes and 5 X 10(8) CFU of B. abortus, S-19, while 3 other groups each received either P. acnes, B. abortus S-19, or saline (s.c.). The antibody titers to B. abortus measured at 6, 10 and 14 weeks after vaccination indicated no significant (P less than 0.01) response in the 2 groups immunopotentiated with P. acnes concurrent with B. abortus S-19 vaccination. The delayed hypersensitivity response to 3 Brucella antigens conducted 8 weeks after immunization did not show a significant difference between the B. abortus S-19 vaccinated group compared with the 2 groups immunopotentiated and vaccinated. However, the proliferative response of lymphocytes to the B. abortus soluble antigen diluted 1:100 indicated significantly enhanced blastogenesis in the (s.c.) immunopotentiated and immunized guinea pigs compared with the B. abortus S-19 vaccinated group. A slightly enhanced response was also observed in the group immunopotentiated (i.v.) and vaccinated (s.c.). The guinea pigs were challenged with B. abortus strain 2308 and necropsied 4 weeks later. The mean splenic CFU of the Brucella in the group immunopotentiated (i.v.) and vaccinated (s.c.) was significantly decreased when compared with the guinea pigs vaccinated with B. abortus S-19 alone. These findings indicated that P. acnes administered simultaneously with B. abortus S-19 vaccine was able to augment the immune response in guinea pigs. Immunomodulation as evidenced by enhanced clearance of B. abortus from the spleens of immunopotentiated animals was presumably brought about by activated macrophages or a T-cell mediated cytolytic mechanism or both.  相似文献   

The December 1997 issue of Agriculture and Human Values     

Richard P. Haynes 《Agriculture and Human Values》1997,14(4):311-312

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Subject-based problem-based learning in the veterinary science course at the University of Queensland     

JS RAND  AJ BAGLIONI jr 《Australian veterinary journal》1997,75(2):120-125

Objective
To assess students' perceptions of subject-based problem-based learning in the fourth year of the veterinary science course at the University of Queensland.
Design
A questionnaire-based study.
Procedure
Subject-based problem-based learning was introduced into parts of two fourth year subjects in a 5-year veterinary science course. The problem-based learning exercise used modified clinical cases and was computer-assisted. Students worked in groups of two to four, and small group discussion sessions were tutorless. Lectures were replaced by large group discussion and feedback sessions, led by the teacher, with approximately 85 students.
Results
There was a significant increase in the percentage of students who strongly agreed that they had better understanding of the subject, and had learned to apply principles from this class in new situations. The only consistent criticism by students was directed at the extra time required compared to traditional lecture-based subjects.
Conclusion
Students' perceptions of the learning outcome were very favourable for problem-based learning when compared to the lecture-based subject.  相似文献   

Direct Comparison of the Effects of Slow Freezing and Vitrification on Late Blastocyst Gene Expression,Development, Implantation and Offspring of Rabbit Morulae     

MD Saenz‐de‐Juano  F Marco‐Jimenez  MP Viudes‐de‐Castro  R Lavara  JS Vicente 《Reproduction in domestic animals》2014,49(3):505-511

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Effect of Exposure to Heatwave During Blastocyst Formation on Reproductive Performance of Female Rabbits     

F Marco‐Jiménez  C Naturil‐Alfonso  DS Peñaranda  E Jiménez‐Trigos  FJ García‐Diego  JS Vicente 《Reproduction in domestic animals》2014,49(4):629-635

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Two Cycles of Recurrent Maternal Half-Sib Selection Reduce Foliar Late Blight in a Diploid Hybrid Solanum phureja-S. stenotomum Population by Two-thirds     

Kathleen G. Haynes  Xinshun Qu  Barbara J. Christ 《American Journal of Potato Research》2014,91(3):254-259

Foliar late blight, caused by Phytophthora infestans, is an important disease problem worldwide. Foliar resistance to late blight was found in a hybrid population of the cultivated diploid species Solanum phureja-S. stenotomum (phu-stn). The objective of this study was to determine if resistance to late blight could be improved by recurrent maternal half-sib selection in the phu-stn population. Four clones from each of 72 maternal half-sib families plus the check cultivar ‘Atlantic’ were evaluated for late blight resistance in replicated field trials in Pennsylvania. The most late blight resistant clone from each of the 72 families was randomly intermated to advance the population. The cycle 1, 2, and 3 populations were evaluated in 1996–1997, 2003–2004, and 2009–2010, respectively. Percent infected foliage was recorded several times towards the end of the growing season and used to compute area under the disease progress curve (AUDPC). Mean AUDPC was 652 (cycle 1), 556 (cycle 2), and 276 (cycle 3), whereas the mean AUDPC of ‘Atlantic’ ranged from 775 to 863 each cycle. Narrow-sense heritability for resistance was estimated as 0.78, 0.77, and 0.80 in cycles 1, 2, and 3, respectively. Recurrent maternal half-sib selection continues to improve the levels of resistance to late blight in this diploid population with no adverse effect on the amount of additive genetic variance. Additional improvements for late blight resistance are likely to occur if this approach is continued.  相似文献   

Reply     

LA CORNER  PR WOOD  JS ROTHEL 《Australian veterinary journal》1991,68(6):218-219

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Epitheliocystis in the leafy sea-dragon   总被引：2，自引：0，他引：2  

JS LANGDON  K. ELLIOTT  B. MACKAY 《Australian veterinary journal》1991,68(7):244-244

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Announcements     

Richard P. Haynes 《Agriculture and Human Values》1993,10(2):76-80

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