用铬变素2R法对家蚕微孢子虫孢子染色的研究   总被引：4，自引：1，他引：3  

唐顺明  张志芳  李奕仁  何家禄 《蚕业科学》2002,28(1):72-74

采用铬变素 2R法 (Chromotrope 2R)染色家蚕微孢子虫 (Nosemabombycis,N .b)孢子和绿僵 (Nomuraearile yi)孢子、曲霉 (Aspergillusflavus)孢子、花粉粒 (Pollengranule)等与N .b孢子形状相似物 ,结果表明 :Chromotrope 2R法特异性地将N .b孢子染成粉红色 ,绿僵孢子、曲霉孢子、花粉粒未被染成红色 ,初步认为Chromotrope 2R法可应用于母蛾镜检的N .b鉴定 ,能明显提高N .b的检出率与准确性 ;染色N .b孢子的最佳时间与温度为 4 0min ,30℃。鉴于本染色法的条件、技术操作简便 ,染色特异性强 ,因而具有良好的应用前景  相似文献   

Leeching as initial treatment in a cat with polycythaemia vera.     

C S Nett  P Arnold  T M Glaus 《The Journal of small animal practice》2001,42(11):554-556

Polycythaemia vera was diagnosed in a three-year-old domestic shorthaired cat referred because of seizures and a high packed cell volume (PCV). Laboratory examination revealed severe erythrocytosis (PCV 79 per cent). Diagnosis was reached by excluding causes for relative and secondary absolute polycythaemia. As phlebotomy proved impossible for initial treatment due to hyperviscosity, four leeches were used to suck blood and the PCV was consequently reduced to 64 per cent. A further 24 hours later, when bleeding at the sites of sucking had stopped, the PCV was 56 per cent. Long-term management of the condition was achieved with hydroxyurea (100 mg/cat once daily) and intermittent phlebotomy. Initial treatment using leeches in cases of polycythaemia vera is a simple, non-invasive, well tolerated and effective method where phlebotomy is not possible.  相似文献   

Rapid communication: linkage mapping of seven bovine cDNA.     

T P Smith  G L Bennett  N Tao  W A Warren 《Journal of animal science》2001,79(6):1631-1632

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Effect of dietary boron on growth performance, calcium and phosphorus metabolism, and bone mechanical properties in growing barrows.   总被引：3，自引：0，他引：3  

T A Armstrong  J W Spears 《Journal of animal science》2001,79(12):3120-3127

An experiment was conducted to evaluate the effects of dietary boron (B) on growth performance, bone mechanical properties, and calcium (Ca) and phosphorus (P) metabolism in pigs. Thirty-six barrows were weaned at approximately 21 d of age and randomly assigned to receive one of three dietary treatments. Treatments consisted of 1) low-B basal diet (control), 2) basal + 5 mg B/kg diet, and 3) basal + 15 mg B/kg diet. Boron was supplemented as sodium borate. Barrows remained on their respective experimental diets throughout the nursery (35 d) and growing (30 d) phases of production. Blood samples were obtained from each barrow at the end of each phase. Following the 30-d growing period, eight barrows per treatment were transferred to stainless steel metabolism crates. Barrows had an adjustment period of 7 d, followed by a 7-d total collection of urine and feces. All barrows were fed at 90% of the previous ad libitum grower intake of the control animals during the adjustment and collection periods. At the end of the 7-d collection period, barrows were killed and femurs and fibulas were harvested for the assessment of bone mechanical properties. During the nursery phase, ADG and ADFI were increased (P < 0.05) by B supplementation. Boron did not affect (P = 0.34) feed efficiency during the nursery phase. During the growing phase, ADG and ADFI were increased (P < 0.05) by B supplementation. Boron did not affect (P = 0.97) feed efficiency during the growing phase. Boron did not affect (P = 0.44) bone ash percentage, but B supplementation increased (P < 0.05) bone ash P. Ultimate shear force of the fibula was increased (P < 0.05) in barrows supplemented with 15 mg B/kg diet compared to barrows fed diets supplemented with 5 mg B/kg diet. Apparent absorption and retention of Ca and P were not affected (P > 0.05) by dietary B. These data indicate that B supplementation to pigs can increase growth and bone strength without greatly affecting Ca and P metabolism.  相似文献   

Morphological study on the stomach of the lesser mouse deer (Tragulus javanicus) with special reference to the internal surface.     

S Agungpriyono  Y Yamamoto  N Kitamura  J Yamada  K Sigit  T Yamashita 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(6):1063-1069

The stomach of the lesser mouse deer (Tragulus javanicus) was observed macroscopically. It consisted of only three compartments, rumen, reticulum and abomasum without omasum. The rumen was S-shaped with large ventral and caudoventral blind sacs and the reticulum was larger than the abomasum. Internally, the rumen was covered with numerous ruminal papillae even on the pillars and the ruminoreticular fold. These papillae were leaf- or tongue-like shaped and varied in size and density. The reticulum had honey-combed crests and the secondary crests were found rarely. The lips of the reticular groove were prominent and more developed in the aboral part than in the oral one. A sac-like transition zone, which had more prominent mucosal folds than had the floor of the reticular groove, was observed between the caudal end of the reticular groove and the abomasum. Mucosal folds of the abomasum were spiral, low but rather thick. These findings were discussed in view of comparison with other ruminants and of possible functional implications.  相似文献   

旱地有限补充供水与覆盖保水效果研究   总被引：1，自引：0，他引：1  

高世铭  朱润身 《干旱区资源与环境》1996,10(2):10-17

本文针对旱地作物长期受水分亏缺制约的问题，为了使有限降水资源得以高效利用，研究了几种作物需水关键期有限补充供水以及覆盖保水的效应，表明旱地有限供水的高效性和对提高经济效益的显著作用。提出了实现有限降水资源富集叠加高效利用建设旱区高产高效农田的技术途径。  相似文献   

Morphine and pethidine antinociception in the crocodile     

T. I. KANUI  K. HOLE 《Journal of veterinary pharmacology and therapeutics》1992,15(1):101-103

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A new intravenous anesthetic agent: propofol.   总被引：2，自引：0，他引：2       下载免费PDF全文

T Duke 《The Canadian veterinary journal. La revue veterinaire canadienne》1995,36(3):181-183

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Suspected Tension Pneumomediastinum In A Dog: A Case Report     

Elke Rudloff DVM  Dennis T. Crowe DVM  Jr. Rebecca Kirby DVM  Bobbie Mammato DVM  MPH 《Journal of Veterinary Emergency and Critical Care》1996,6(2):103-107

Successful resuscitation of a trauma-induced, suspected tension pneumomediastinum in a dog is presented. The cause was a caudal cervical penetration wound which acted as a one-way valve allowing air to enter, but not exit, the cranial mediastium. Early in the course of hospitalization, respiratory and cardiovascular collapse occurred; an emergent parasternotomy allowed decompression of the mediastinum, and the cradiovascular system immediately responded. The dog made a complete recovery following surgical exploration and removal of contaminated mediastinal tissue. Nine days of intensive care included 24-hour monitoring, oxygen therapy, intravenous crystalloids and colloids, continuous pleural suctioning, nutritional support, antibiotics, and pain control.  相似文献   

杉木林地套种绿肥筛选初报   总被引：4，自引：1，他引：3  

马祥庆  何智英 《福建林学院学报》1992,12(3):343-346

收集了三十种绿肥,在杉木当年造林地上进行套种试验,根据其生长状况、生物量、覆盖度及对杉木生长的影响,把供试绿肥分成三类,初步筛选出羽扁豆、决明豆、日木草为适宜杉木林地种植的林用绿肥。同时对今后林用绿肥的选择提出了有益的建议。  相似文献