Structure of the genes for porcine endometrial secreted and membrane folate binding proteins     

Vallet JL  Smith TP  Sonstegard TS  Heaton M  Fahrenkrug SC 《Domestic animal endocrinology》2001,21(1):55-72

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Infection of cattle in Queensland with bluetongue viruses: II. distribution of antibodies     

MP WARD  TE CARPENTER† 《Australian veterinary journal》1995,72(6):217-220

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Trends in the use of pesticides and pestiside residues on Queensland wool     

MP WARD  RTF ARMSTRONG 《Australian veterinary journal》1998,76(10):694-697

Objective To determine the amounts of organophosphorous and synthetic pyrethroid residues on Queensland wool sampled between 1995 and 1997, and to study if pesticide use changed during the period.
Design Estimated amounts of residues were obtained from a survey of Queensland wool clips, and information on pesticide use was obtained from a trace-back postal survey of flock managers.
Procedure Trends in amounts of residues over time were assessed using analysis of variance and simple linear regression models, and changes in pesticide use was assessed using x² tests.
Results Significant linear reductions in organophosphorous (P = 0.0012), synthetic pyrethroid (P = 0.0044) and total (P = 0.0002) residues were detected. The proportion of woolgrowers treating for louse infestation (P = 0.0046) treating twice or more (P = 0.0006) and treating more than 4 months after shearing (P = 0.0001) decreased between 1994 and 1997. However, the proportion of growers who treated for blowfly strike (P = 0.0124) and used insect growth regulator pesticides increased (P < 0.0001). The use of handjetting to apply pesticides for blowfly strike control increased and the use of dips decreased (P < 0.0001).
Conclusion Residues of organophosphorous compounds and synthetic pyrethroids reduced in Queensland wool between 1994 and 1997. Although woolgrowers treated for louse infestation less, pesticide use to control blowfly strike increased. The increasing use of insect growth regulators in the industry needs to be monitored.  相似文献   

Escherichia coli O157:H7 – Discerning Facts from Fiction: An Integrated Research and Extension Project for Multiple Audiences          下载免费PDF全文

D. A. Moore  D. R. Smith  W. M. Sischo  K. Heaton  T. E. Besser 《Zoonoses and public health》2016,63(1):72-81

The O157:H7 (EcO157) epidemiology of Shiga‐toxin‐producing Escherichia coli (STEC) in cattle is complex, and myths about pre‐harvest control are perpetuated. The objectives of this project were to identify perpetuated misinformation and inform four audiences about evidence‐based risks and pre‐harvest control of EcO157 by addressing: (i) EcO157 epidemiology and pre‐harvest control; (ii) how food safety policy is created; and (iii) how to present accurate information about EcO157. An environmental scan using a daily Internet search helped identify themes for education. A literature review of pre‐harvest control measures contributed to the development of educational materials (fact sheets, website, web presentations and conferences). Conference 1 was a webinar with 315 registrants, 10 countries including 41 US states and four Canadian provinces. Most participants felt confident in using their new knowledge, more than half felt confident enough to answer EcO157 questions from the public and many would recommend the recorded version of the webinar to colleagues. Conference 2 was live in the Washington, DC, area with most participants employed by the US government. All agreed that they better understood pre‐harvest control, how food safety policy was made, and were confident they could create an effective message about STEC pre‐harvest control. Videos were posted and received 348 Internet visitors within 2 months. Conference 3 was a webinar with a live audience and Twitter feeds, targeting people who give nutrition advice. Almost all ranked the programme good to excellent and relevant to their work. About 25% indicated that they would share: ‘grass‐fed beef is not safer than grain‐fed’, 25% would share information on effectiveness of cattle vaccines, and 14% would share information on message mapping. Across all conferences, major changes in knowledge included the following: there is no additional risk of EcO157 shedding from grain‐fed versus grass‐fed cattle, pre‐harvest vaccination is efficacious, and production systems (pasture versus confinement) do not affect EcO157 shedding rates.  相似文献   

Confirmed case of Pneumocystis pneumonia in a Maltese Terrier × Papillon dog being treated with toceranib phosphate     

MP Best  SP Boyd  P Danesi 《Australian veterinary journal》2019,97(5):162-165

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Epidemiology and clinical presentation of canine distemper disease in dogs and ferrets in Australia, 2006–2014          下载免费PDF全文

SE Wyllie  M Kelman  MP Ward 《Australian veterinary journal》2016,94(7):215-222

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Can the Genetic Origin Affect Rabbit Seminal Plasma Protein Profile along the Year?          下载免费PDF全文

L Casares‐Crespo  AM Talaván  MP Viudes‐de‐Castro 《Reproduction in domestic animals》2016,51(2):294-300

The study was designed to evaluate the influence of genetic origin on rabbit seminal plasma protein profile variation along the year. Seminal plasma of rabbits from line A (maternal line) and R (paternal line) collected during a natural year was subjected to polyacrylamide gel electrophoresis (SDS‐PAGE). The electrophoretic profile of rabbit seminal plasma resulted in multiple protein bands of different intensity ranging from 9 to 240 kDa. Results showed that seven protein bands were significantly different between genetic lines, and among these, three protein bands were significantly different between seasons. The differentially expressed proteins were identified by MALDI‐TOF/TOF or LC‐MS/MS analysis and were the following ones: FAM115E‐like (220, 113 and 59 kDa), ectonucleoside triphosphate diphosphohydrolase 3 isoform X2 (72 kDa), annexin A5 (32 kDa), lipocalin allergen Ory c 4 precursor (19 kDa), and haemoglobin subunit zetalike (13 kDa) between genetic lines and FAM115E‐like (113 kDa), haemoglobin subunit zetalike (13 kDa) and β‐nerve growth factor (12 kDa) between seasons. These results indicate that proteins from rabbit seminal plasma are under both seasonal control and genetic control. Furthermore, the differential presence of these proteins could be one of the causes explaining the differences observed in fertility and seminal parameters between these two lines in earlier studies.  相似文献   

Mechanistic target of rapamycin is activated in bovine granulosa cells after LH surge but is not essential for ovulation          下载免费PDF全文

PRA da Rosa  AMP Dau  MP De Cesaro  JT dos Santos  BG Gasperin  R Duggavathi  V Bordignon  PBD Gonçalves 《Reproduction in domestic animals》2016,51(5):766-773

The LH surge induces functional and morphological changes in granulosa cells. Mechanistic target of rapamycin (mTOR) is an integrator of signalling pathways in multiple cell types. We hypothesized that mTOR kinase activity integrates and modulates molecular pathways induced by LH in granulosa cells during the preovulatory period. Cows were ovariectomized and granulosa cells collected at 0, 3, 6, 12 and 24 hr after GnRH injection. While RHEB mRNA levels increased at 3 and 6 hr, returning to basal levels by 12 hr after GnRH treatment, RHOA mRNA levels increased at 6 hr and remained high thereafter. Western blot analyses revealed increased S6K phosphorylation at 3 and 6 hr after GnRH injection. Similarly, mRNA levels of ERK1/2, STAR and EGR‐1 were higher 3 hr after GnRH treatment. Rapamycin treatment inhibited mTOR activity and increased AKT activity, but did not alter ERK1/2 phosphorylation and EGR1 protein levels in cultured bovine granulosa cells. Rapamycin also inhibited LH‐induced increase in EREG mRNA abundance in granulosa cells in vitro. However, intrafollicular injection of rapamycin did not suppress ovulation. These findings suggest that mTOR is involved in the control of EREG expression in cattle, which may be triggered by LH surge stimulating RHEB and S6K activity.  相似文献   

Survey of Australian equine veterinarians evaluating their biosecurity training and perceptions and opinions about the management of the 2007 equine influenza outbreak     

K Schemann  J‐ALML Toribio  MR Taylor  MP Ward  NK Dhand 《Australian veterinary journal》2014,92(4):93-100

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Primary immune‐mediated thrombocytopenia and immune‐mediated neutropenia suspected in a 21‐week‐old Maine Coon cat     

MP Best  DR Fry 《Australian veterinary journal》2014,92(7):250-253

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