Prevalence of Bartonella species,Rickettsia felis,haemoplasmas and the Ehrlichia group in the blood of cats and fleas in eastern Australia     

VR Barrs  JA Beatty  BJ Wilson  N Evans  R Gowan  RM Baral  AE Lingard  G Perkovic  JR Hawley  MR Lappin 《Australian veterinary journal》2010,88(5):160-165

Objectives To define the prevalence of Bartonella spp., Rickettsia felis, Mycoplasma haemofelis, ‘Candidatus Mycoplasma haemominutum’ (Mhm) and ‘Candidatus Mycoplasma turicensis’ (Mtc) in cats and their fleas in eastern Australia. Design and procedure Conventional PCR assays that detect Bartonella spp., M. haemofelis, Mhm, Mtc, Rickettsia spp., Ehrlichia spp., Anaplasma spp. and Neorickettsia spp. were performed on DNA extracted from blood and fleas collected from 111 cats. Cat sera were assayed by ELISA for IgG of Bartonella spp. Results DNA of M. haemofelis, Mtc and Mhm was amplified from 1 (0.9%), 1 (0.9%) and 17 cats (15.3%), respectively. Only DNA of Mhm was amplified from the 62 of 111 pooled flea samples (flea sets; 55.9%). Overall, the prevalence rates for Bartonella spp. DNA in the cats and the flea sets was 16.2% (18 cats) and 28.8% (32 flea sets), respectively. Bartonella spp. IgG was detected in 42 cats (37.8%), of which 11 (26.2%) were positive for Bartonella spp. DNA in their blood. R. felis DNA was amplified from 22 flea sets (19.8%), but not from cats. Overall, DNA of one or more of the organisms was amplified from 27% (30) of cats and 67.6% (75) of the flea sets. Conclusions This is the first Australian study to determine the prevalence of R. felis and B. clarridgeiae in both fleas and the cats from which they were collected. Flea-associated infectious agents are common in cats and fleas in eastern Australia and support the recommendation that stringent flea control be maintained on cats.  相似文献   

Some haematological data for red deer (Cervus elaphus) in England     

D H Upcott  C N Hebert 《The Veterinary record》1965,77(46):1348-1349

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A radiographic study of development of the appendicular skeleton in the fetal pig     

A E Wrathall  J Bailey  C N Hebert 《Research in veterinary science》1974,17(2):154-168

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The role of effluent monitoring in environmental control     

Peter N. Nemetz  Hebert D. Drechsler 《Water, air, and soil pollution》1978,10(4):477-497

Using effluent data from a British Columbia pulp mill, the authors demonstrate certain critical deficiencies associated with currently emplaced monitoring systems. The principal ingredient in effective environmental control is information, and current monitoring regimes entail an unacceptable level of information loss. Composite sampling systems fail to provide the two key types of information required for effective environmental control: (i) a high representational accuracy of the effluent profile; and (ii) the detection of most or all pollutant releases with potentially serious environmental consequences. In light of these conclusions, the authors make several recommendations concerning current and alternative sampling regimes.  相似文献   

Porcine congenital tremor type AII: spinal cord morphometry     

J T Done  J Woolley  D H Upcott  C N Hebert 《The British veterinary journal》1986,142(2):145-150

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Comparative biological and biochemical studies in hybrid chicks. 3. Vaccination with inactivated newcastle disease vaccine and its effect on serum protein concentrations     

D S Patterson  D Sweasey  C N Hebert  R B Carnaghan 《British poultry science》1967,8(4):285-287

The potency of an inactivated Newcastle disease vaccine containing a number of strains of virus (vaccine 1) was greater in 28‐day old‐chicks of a hybrid strain (hybrid A) than in Rhode Island Red X Light Sussex chicks. Vaccination with one PD50 of vaccine 1 or vaccine 2 (a single strain vaccine) produced only minor changes in the electro‐phoretically determined serum protein composition in two hybrid strains (A and B) at a time when maximum immunity had developed.  相似文献   

Atrazine nanoencapsulation improves pre‐emergence herbicidal activity against Bidens pilosa without enhancing long‐term residual effect on Glycine max     

Ana C Preisler  Anderson ES Pereira  Estefnia VR Campos  Giliardi Dalazen  Leonardo F Fraceto  Halley C Oliveira 《Pest management science》2020,76(1):141-149

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Expression of the chicken lysozyme gene in potato enhances resistance to infection byErwinia carotovora subsp.atroseptica     

Carolina Serrano  Patricio Arce-Johnson  Hebert Torres  Marlene Gebauer  Mónica Gutierrez  Mauricio Moreno  Xavier Jordana  Alejandro Venegas  Julio Kalazich  Loreto Holuigue 《American Journal of Potato Research》2000,77(3):191-199

Infection of potato plants and tubers with the bacteriumErwinia carotovora subsp.atroseptica produces blackleg and soft rot diseases, which cause significant losses to crops and stored potatoes. In order to obtain resistance against this bacterium, the genechly encoding the enzyme lysozyme from chicken was introduced into potato plants (cv. Desirée) viaAgrobacterium- mediated transformation. Sixty-three and 69 transgenic potato clones were evaluated in the greenhouse for resistance to blackleg and soft rot diseases, respectively. Results reported in this paper indicate that 21%-29% of the potato clones showed increased resistance to infection by the bacteriumE. c. subsp.atroseptica T7, as revealed by a reduced severity of blackleg or soft rot symptoms. Nine clones showing different levels of resistance were selected for further molecular analysis. The number of copies of the transgene integrated in the plant genome of these clones was estimated by Southern blot analysis. The level of transgene expression, detected by Northern blot analysis, correlated with the level of resistance detected in these clones.  相似文献   

Early forebrain wiring: genetic dissection using conditional Celsr3 mutant mice     

Zhou L  Bar I  Achouri Y  Campbell K  De Backer O  Hebert JM  Jones K  Kessaris N  de Rouvroit CL  O'Leary D  Richardson WD  Goffinet AM  Tissir F 《Science (New York, N.Y.)》2008,320(5878):946-949

Development of axonal tracts requires interactions between growth cones and the environment. Tracts such as the anterior commissure and internal capsule are defective in mice with null mutation of Celsr3. We generated a conditional Celsr3 allele, allowing regional inactivation. Inactivation in telencephalon, ventral forebrain, or cortex demonstrated essential roles for Celsr3 in neurons that project axons to the anterior commissure and subcerebral targets, as well as in cells that guide axons through the internal capsule. When Celsr3 was inactivated in cortex, subcerebral projections failed to grow, yet corticothalamic axons developed normally, indicating that besides guidepost cells, additional Celsr3-independent cues can assist their progression. These observations provide in vivo evidence that Celsr3-mediated interactions between axons and guidepost cells govern axonal tract formation in mammals.  相似文献   

Methodology for quantifying residues of chlorhexidine in raw dairy milk   总被引：1，自引：0，他引：1  

Hebert VR  Middleton JR  Tomaszewska E  Fox LK 《Journal of agricultural and food chemistry》2003,51(3):567-570

A residue method was developed as part of a pharmacokinetics study to determine the elimination of chlorhexidine in raw milk after intramammary infusion into dairy cows affected with bovine mastitis. The developed liquid/liquid and solid-phase extraction procedures effectively reduced sources of milk product interferences in the final extract. By optimizing mobile-phase pH buffer/acetonitrile gradient conditions and employing an end-capped reverse-phase polar embedded-phase chromatographic column, excellent peak resolution was achieved without the additional need of mobile-phase amine modifiers or ion-pairing reagents. The combined cleanup and chromatographic method steps reported herein were sensitive and reliable for determining the pharmacokinetic elimination of chlorhexidine following intramammary infusion. The residue method was found to be rugged with a lower detection limit of 0.1 ppm.  相似文献