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51.
An epizootic of eastern equine encephalomyelitis in Connecticut 总被引:1,自引:0,他引:1
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Incidence of tenosynovitis in Maine broilers 总被引:3,自引:0,他引:3
56.
Growth and dormancy induction of seedlings or micropropagated plants of three Prunus species were studied under controlled environment conditions. All the species tested, P. cerasus L. and P. insititia L. (two cultivars each), and P. avium L. were insensitive to photoperiod at high temperature and maintained continuous growth in both 10 and 24-h photoperiods at 21 °C. At lower temperatures, however, growth was controlled by the interaction of photoperiod and temperature, the species and cultivars varying somewhat in their responses. At 9 °C growth cessation was induced regardless of day-length conditions in the plum rootstocks ‘St. Julien A’ and ‘Weito’ as well as in the sour cherry rootstock ‘Weiroot’, whereas in the sour cherry rootstock ‘Gisela 5’ growth cessation took place in short day (SD) only. At intermediate temperatures (12 and 15 °C) growth cessation occurred in SD only in both sour cherry cultivars. In P. avium seedlings on the other hand, growth cessation in SD was only induced at 9 °C, continuous but reduced growth taking place also in SD at all higher temperatures. Growth rates increased progressively with increasing temperature under long day (LD) conditions in all species, and this was associated with increased internode length in LD compared with SD conditions. Production of new leaves was unaffected by photoperiod at high temperature, but was higher in LD than in SD at lower temperatures. After growth cessation at low temperature the plants developed winter buds and became dormant also in LD conditions. These results demonstrate that, like several species of the Pomoidae subfamily of the Rosaceae, these Prunus species are insensitive to short photoperiods at relatively high temperatures. However, the photoperiodic response of the Prunus species is highly temperature dependent, and the transition temperatures for shifts in the photoperiodic response mode vary among the species. 相似文献
57.
Cees Waalwijk Ruth van der Heide Ineke de Vries Theo van der Lee Cor Schoen Guillaume Costrel-de Corainville Isolde Häuser-Hahn Pieter Kastelein Jürgen Köhl Philippe Lonnet Thierry Demarquet Gert H.J. Kema 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(5-6):481-494
Fusarium head blight (FHB) of wheat and other small-grain cereals is a disease complex caused by several fungal species. To monitor and quantify the major species in the FHB complex during the growing season, real-time PCR was developed. TaqMan primers and probes were designed that showed high specificity for Fusarium avenaceum, F. culmorum, F. graminearum, F. poae and Microdochium nivale var. majus. Inclusion of an internal PCR control and serial dilutions of pure genomic DNAs allowed accurate determination of the concentration of fungal DNA for each of these species in leaves, ears as well as harvested grains of winter wheat. The DNA concentration of F. graminearum in grain samples correlated (r 2= 0.7917) with the incidence of this species on the grain as determined by isolation from individual kernels. Application of the TaqMan technology to field samples collected in 40 wheat crops in the Netherlands during the growing season of 2001 revealed that M. nivale var. majus predominated on leaves early in the season (GS 45-65). Ears and harvested grains from the same fields, however, showed F. graminearum as the major species. In 2002, grain samples from 40 Dutch fields showed a much wider range of species, whereas in ears from 29 wheat crops in France, F. graminearum was the predominant species. The concentration of DON correlated equally well with the incidence of the DON-producing species F. culmorum and F. graminearum in the grain samples (r 2= 0.8232) as well as with total DNA of both these species (r 2= 0.8259). The Fusarium TaqMan technology is an important tool to quantify and monitor the dynamics of individual species of the complex causing FHB in cereals during the growing season. This versatile tool has been applied in a comparison of different genotypes, but can also be applied to other disease management systems, e.g. fungicide treatments. 相似文献
58.
The effects of timing of nitrogen (N) fertilization relative to the beginning of a 4-week floral-inducing short-day (SD) period have been studied in ‘Korona’ strawberry plants under controlled environment conditions. Groups of low fertility plants were fertilized with 100 ml of calcium nitrate solution for 3 days a week for a period of 3 weeks starting at various times before and at the beginning of the SD period, as well as at different times during the SD period. All plants, including SD and long day (LD) control plants, received a weekly fertilization with a low concentration complete fertilizer solution throughout the experiment. Leaf area, fresh and dry matter increments of leaves, crowns and roots, as well as leaf chlorophyll concentration (SPAD values) were monitored during the experimental period. A general enhancement of growth took place at all times of N fertilization. This was paralleled by an increase in leaf chlorophyll concentration, indicating that the control plants were in a mild state of N deficiency. When N fertilization was started 2 weeks before beginning of the SD period, flowering was delayed by 7 days, and this was gradually changed to an advancement of 8 days when the same treatment was started 3 weeks after the first SD. The amount of flowering was generally increased by N fertilization although the effect varied greatly with the time of N application. The greatest flowering enhancement occurred when N fertilization started 1 week after the first SD when the number of flowering crowns and the number of inflorescences per plant were more than doubled compared with the SD control, while fertilization 2 weeks before SD had no significant effect on these parameters. Importantly, the total number of crowns per plant was not affected by N fertilization at any time, indicating that enhancement of flowering was not due to an increase in potential inflorescence sites. No flowering took place in the control plants in LD. Possible physiological mechanisms involved and practical applications of the findings are discussed. 相似文献
59.
The objective of this study was to evaluate the relative efficacy of otoscope cone cleaning and disinfection methods commonly used in veterinary practices. Using sterile technique, 60 new gas-sterilized 4-mm otoscope cones were inoculated with a broth culture of 1.5 billion Pseudomonas aeruginosa bacteria per mL then allowed to dry for 10 min. Six study groups of 10 cones each were created. Group 1 served as positive control and received no cleaning or disinfection. Group 2 cones were wiped with sterile cotton-tipped applicators and gauze then rinsed with water. Group 3 cones were wiped with 70% isopropyl alcohol. Group 4 cones were scrubbed in a speculum cleaner with Cetylcide II solution (Cetylite Industries, Inc., Pennsauken, NJ). Groups 5 and 6 cones were soaked for 20 min in Cetylcide II and chlorhexidine gluconate 2% solutions, respectively. Using sterile technique and after 10-15 min drying time, the cones were swabbed in a consistent pattern, and samples were submitted for quantitative culture. Culture results showed no growth from cones soaked in Cetylcide II or chlorhexidine solutions. Two of the 10 cones wiped with alcohol, 3/10 cones wiped then rinsed with water, and 3/10 cones scrubbed with the speculum cleaner showed growth of P. aeruginosa. All (10/10) cones in the control group showed heavy growth of P. aeruginosa. These results show that P. aeruginosa can survive on otoscope cones cleaned and disinfected by several commonly used methods. Further study is needed to determine practical and optimal cleaning and disinfection methods for otoscope cones. 相似文献
60.
Additional Indications for the Low Allergenic Properties of the Apple Cultivars Santana and Elise 总被引:1,自引:1,他引:0
B. J. Vlieg-Boerstra W. E. van de Weg S. van der Heide I. Skypala P. Bures B. K. Ballmer-Weber K. Hoffmann-Sommergruber D. Zauli G. Ricci A. E. J. Dubois 《Plant foods for human nutrition (Dordrecht, Netherlands)》2013,68(4):391-395
Patients with Oral Allergy Syndrome (OAS) to fresh apple may tolerate low allergenic apple cultivars. We aimed to investigate if the low allergenic properties of Elise and Santana, as previously identified in a Dutch population, could be generalised within North West Europe within the birch pollen region with regard to both the prevalence and degree of sensitization. Prick-to-prick tests (PTP) were performed in eighty-five adult patients with OAS to fresh apple in Great Britain, Switzerland and Northern Italy, before the birch pollen season, using the putatively low allergenic apple cultivars Elise, Santana, Granny Smith, Modi and Mcintosh, as well as the putatively high allergenic apple cultivars Golden Delicious and Kanzi. No significant differences in percentages of negative responses of PTPs were found between the three countries. Negative responses did not differ from negative responses to the different apple cultivars we previously found in 2006/2007 in the Netherlands. The size of the PTPs of all apple cultivars tested were correlated to the size of the skin prick tests with birch pollen. These results add to the indications for the low allergenic properties of the low allergenic apple cultivars Santana and Elise, as the number of negative responses were reproducible in three countries within the birch pollen region and were similar to previous results in the Netherlands. These results justify oral challenge studies with Elise and Santana within the birch pollen region, to establish the low allergenic properties for the benefit for apple allergic consumers for definite conclusions. 相似文献