Sesamolinol glucoside, disaminyl ether, and other lignans from sesame seeds     

Grougnet R  Magiatis P  Laborie H  Lazarou D  Papadopoulos A  Skaltsounis AL 《Journal of agricultural and food chemistry》2012,60(1):108-111

The application of a procedure based on XAD-4 adsorption resin permitted the obtainment of an enriched polyphenolic extract from Sesamum indicum seeds. Chemical analysis of the obtained extract led to the identification of 12 lignans. Among them, 2 lignans, (+)-sesamolinol-4'-O-β-D-glucoside and disaminyl ether, are reported for the first time as natural compounds. Their structure has been determined by spectroscopic methods, mainly by the application of one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) techniques [heteronuclear multiple-quantum coherence (HMQC), heteronuclear multiple-bond correlation (HMBC), and nuclear Overhauser effect spectrometry (NOESY)] and mass spectroscopy. The isolated compounds were evaluated for their antimutagenic activity. Among the tested lignans, the most active lignan was found to be sesamolin, followed by sesamolinol and samin, against H(2)O(2). Additionally, some of the tested lignans showed desmutagenic activity against benzo[a]pyrene (BaP).  相似文献   

The effect of intermittent hypoxia on growth,appetite and some aspects of the immune response of Atlantic salmon (Salmo salar)     

Kimberly Burt  Dounia Hamoutene  Juan Perez‐Casanova  Anthony Kurt Gamperl  Helene Volkoff 《Aquaculture Research》2013,45(1):124-137

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Accumulation of mixed linkage (1 → 3) (1 → 4)-β-d-glucan during grain filling in barley: A vibrational spectroscopy study     

Helene Fast Seefeldt  Andreas Blennow  Birthe Møller Jespersen  Bernd Wollenweber  Søren Balling Engelsen 《Journal of Cereal Science》2009

The accumulation of mixed linkage barley (1 → 3) (1 → 4)-β-d-glucan (BG) during grain filling at eight stages was studied using standard reference methods and infrared spectroscopy. Two mutant barley genotypes having higher (starch mutant lys5f) and lower (high lysine mutant lys3a) BG content than the normal control Cork were studied. The Cork and lys3a genotypes showed a linear BG accumulation throughout the grain filling to reach a maximum of approximately 6 and 4% BG (w/w) dry matter, respectively. However, lys5f mutant exhibited an exponential increase in BG synthesis to a maximum of approximately 18% BG (w/w) dry matter 30 days after flowering (DAF), seemingly compensating for a decreased synthesis of starch.  相似文献   

Poinsettia protoplasts - a simple, robust and efficient system for transient gene expression studies     

Pitzschke A  Persak H 《Plant methods》2012,8(1):14

ABSTRACT: BACKGROUND: Transient gene expression systems are indispensable tools in molecular biology. Yet, their routine application is limited to only few plant species and often requires substantial equipment and facilities. The high content of chloroplasts and chlorophyll can impede downstream applications of transformed cells from green plant tissue. RESULTS: We describe a fast and simple technique for the high-yield isolation and efficient transformation (>70%) of mesophyll-derived protoplasts from red leaves of the perennial plant Poinsettia (Euphorbia pulccherrima) for which no particular growth facilities or expensive equipment are needed. Poinsettia protoplasts display an astonishing robustness and can be employed in all commonly-used downstream applications, such as subcellular localisation (multi-colour fluorescence) or promoter activity studies. Due to a low abundance of chloroplasts or chromoplasts, problems encountered in other mesophyll-derived protoplast systems (particularly autofluorescence) are alleviated. Transgene expression is detectable within 90 min post-transformation and lasts over several days. CONCLUSIONS: The simplicity of isolation and transformation renders Poinsettia protoplasts an attractive system for transient gene expression experiments, including multicolour fluorescence, subcellular localisation and promoter activity studies. In addition, they offer hitherto unknown possibilities for anthocyan research and industrial applications.  相似文献   

Use of Nitinol Stents for End‐Stage Tracheal Collapse in Dogs     

April M. Durant DVM  Patricia Sura DVM  MS  Diplomate ACVS  Barton Rohrbach VMD  MPH  Diplomate ACVPM  Mark W. Bohling DVM  PhD  Diplomate ACVS 《Veterinary surgery : VS》2012,41(7):807-817

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What is your diagnosis? A mass on the right tarsus in a rabbit          下载免费PDF全文

Lilani Munasinghe  Christina Dougherty  Santhi Sridharan  Helene Philibert  Beverly Kidney 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2017,46(1):187-188

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Cover Crops and Soil Phosphorus Availability     

Moniki Campos Janegitz  Ana Rita Helene Martins 《Communications in Soil Science and Plant Analysis》2017,48(10):1240-1246

Plants affect soil phosphorus (P) solubility through root exudates, but studies are lacking on species used as relay or cover crops in tropical environments. We evaluated the effect of cover crops on soil phosphorus (P) availability in an oxisol. Ruzigrass (Brachiaria ruziziensis), pearl millet (Pennisetum glaucum), peanut (Arachis hypogaea), crambe (Crambe abyssinica), and sorghum (Sorghum bicolor) were grown in pots with soil. Phosphorus uptake, soil inorganic and organic P, maximum P adsorption capacity, and plant root systems were assessed. When root length density is high, the efficiency of P uptake is low due to root competition. Crambe results in greater soil P availability, while peanut and sorghum decrease the soil maximum P adsorption capacity, probably by exuding or stimulating microbial production of organic acids and phenolic compounds. Hence, crambe, peanut, and sorghum are species that may be of interest to increase P use efficiency in cropping systems.  相似文献   

Lipid composition and deposition during grain filling in intact barley (Hordeum vulgare) mutant grains as studied by H HR MAS NMR     

Helene Fast SeefeldtNanna Viereck  Mikael Agerlin PetersenSøren Balling Engelsen 《Journal of Cereal Science》2011,54(3):442-449

Conventional barley has an excellent nutritional value, but a lower content of lipids than oat. Nevertheless, barley lipids deserve a focused interest due to their fatty acid composition and their vitamin E composition. In this study, ¹H HR MAS NMR was used to monitor the deposition of lipids during grain filling of conventional barley, and of two barley endosperm mutants with increased lipid content. The lipids in the mutants are primarily synthesized between days 9 and 13, whereas the lipid biosynthesis in the conventional barley took place ten days later. GC analysis on barley flour lipids showed that the mutants and the control exhibit comparable relative levels of the major fatty acids: C16:0, C18:1 and C18:2 despite the higher content of lipids in the mutants. Differences in the minor fatty acid composition between control and mutants were also observed. The degree of unsaturation was found to be increasing during grain filling for both conventional barley and the two mutants.  相似文献   

Variability of estradiol concentration in normal dogs     

Linda A. Frank  Rebekah Mullins  Barton W. Rohrbach 《Veterinary dermatology》2010,21(5):490-493

Estradiol concentrations are evaluated in canine serum as part of an adrenal panel used to diagnose atypical Cushing’s syndrome and other endocrine abnormalities. Estradiol concentrations are often elevated in dogs without clinical signs of hyperestrogenism, and the significance of this elevation is unknown. The purpose of this study was to estimate the variation in estradiol concentrations in normal dogs. Ten neutered male and female dogs were enrolled in the study. Blood was collected from each dog at 2 h intervals, four times during a given day. This was repeated approximately 1 (week 2) and 5 weeks later (week 6). There was no attempt for a given dog to be started at the exact time or day each week. Results showed that estradiol concentrations ranged from 44.6 to 120.3 pg/mL with a mean of 70.4 pg/mL, which is greater than the upper limit of normal for our laboratory (69 pg/mL). The mean difference between the highest and lowest concentrations for each dog was 28.8 pg/mL, with a range of 12.5–53.5 pg/mL. Mean estradiol concentrations from week 6 (63.2 pg/mL) were significantly lower than those from week 1 (71.4 pg/mL; P = 0.015) and week 2 (76.5 pg/mL; P = 0.0004). These data show a wide range of variability in estradiol concentration both within and between dogs and that these measurements often exceed the normal ranges established by the laboratory. Therefore, diagnosis of hyperestrogenism or atypical Cushing’s syndrome based on increased estradiol concentrations should require compatible clinical presentation of hyperestrogenism together with elevated serum estradiol.  相似文献   

European outbreak of atypical myopathy in the autumn 2009     

Gaby Van Galen DVM  MSc  DECEIM  Helene Amory DVM  PhD  DECEIM  Evita Busschers DVM  DACVS  Dominique Cassart DVM  PhD  Marco De Bruijn DVM  DECEIM  Vincent Gerber DVM  PhD  DECEIM  John Keen DVM  MSc  DECEIM  Laurence Lefere DVM  Christel Marcillaud Pitel DVM  Celia Marr DVM  PhD  DECEIM  Jessica‐M. V. Müller DVM  DECEIM  Xavier Pineau DVM  Claude Saegerman PhD  Charlotte Sandersen DVM  PhD  DECEIM  Didier Serteyn DVM  PhD  DECVAA  Sara Torfs DVM  Lucia Unger DVM  Denis Verwilghen DVM  MSc  DECVS  Dominique‐Marie Votion DVM  PhD 《Journal of Veterinary Emergency and Critical Care》2010,20(5):528-532

Background – Atypical myopathy is an acute, severe rhabdomyolysis occurring in grazing horses. In the beginning of October 2009, a new outbreak occurred in several European countries. Geographic, demographic and clinical data of the reported cases in the month October 2009 are described. Key Findings – The survival rate in this outbreak was 25%. The most frequently observed clinical signs were congested mucous membranes, dyspnea, tachycardia, depression, weakness, stiffness, recumbency, trembling, sweating, and myoglobinuria. Nonsurvivors were significantly more likely to be recumbent than survivors. Prognostic factors, symptomatic treatment, and preventive measures are discussed. Significance – Differences were encountered during the described outbreak of atypical myopathy in October 2009 compared with previous outbreaks reported. Equine practitioners should be aware that previous epidemiological studies have shown that after a high prevalence in the autumn, new cases are likely to occur in the following spring.  相似文献