Consequences for the bovine embryo of being derived from a spermatozoon subjected to post-ejaculatory aging and heat shock: development to the blastocyst stage and sex ratio     

Hendricks KE  Martins L  Hansen PJ 《The Journal of reproduction and development》2009,55(1):69-74

The objective was to determine whether aging of sperm caused by incubation at normothermic (38.5 C) or heat shock (40 C) temperatures for 4 h prior to oocyte insemination affects sperm motility, fertilizing ability, competence of the resultant embryo to develop to the blastocyst stage and blastocyst sex ratio. In the first experiment, the percent of sperm that were motile was reduced by aging (P<0.001) and the reduction in motility was greater for sperm at 40 C compared to sperm at 38.5 C (P<0.01). In the second experiment, oocytes were inseminated with aged sperm. A smaller percent of oocytes fertilized with sperm aged at either temperature cleaved by Day 3 after insemination than oocytes fertilized with fresh sperm (P<0.05). There was no effect of sperm aging on the percent of oocytes or cleaved embryos that developed to the blastocyst stage. Aging of sperm before fertilization at 38.5 C reduced the percent of blastocysts that were male (P=0.08). In the third experiment, incubation of sperm at 38.5 C or 40 C for 4 h did not reduce fertilizing ability of sperm as determined by pronuclear formation at 18 h post insemination. In conclusion, aging of sperm reduced cleavage rate and the percent of blastocysts that were males but had no effect on the developmental capacity of the embryo. The effect of aging on cleavage rate may represent reduced motility and errors occurring after fertilization and pronuclear formation. Aging at a temperature characteristic of maternal hyperthermia had little additional effect except that polyspermy was reduced. Results indicate that embryo competence for development to the blastocyst stage is independent of sperm damage as a result of aging for 4 h at normothermic or hyperthermic temperatures.  相似文献   

An evaluation of the protein quality of quinoa.     

A W Mahoney  J G Lopez  D G Hendricks 《Journal of agricultural and food chemistry》1975,23(2):190-193

  相似文献   

Fumonisin in tortillas produced in small-scale facilities and effect of traditional masa production methods on this mycotoxin   总被引：1，自引：0，他引：1  

De La Campa R  Miller JD  Hendricks K 《Journal of agricultural and food chemistry》2004,52(14):4432-4437

Four small tortilla plants were visited in Cameron County, Texas, where observations were made on their production methods. Samples of liquids and solids were collected at each stage of the nixtamalization process, and the pH was recorded. Samples were analyzed for fumonisin B(1) (FB(1)) using an immunoaffinity column/HPLC method chosen for its sensitivity for FB(1). It was found that production methods were highly variable among the producers visited, with major differences particularly in the amount of lime added and boiling times. As reported by others working in Mexico and Central America, FB(1) was found in some tortillas. This led to studies of the effects of the various recipes and across a greater range of initial FB(1) concentration/damaged corn than has typically been reported. Five initial concentrations of FB(1) were tested using irradiated corn kernels inoculated with Fusarium verticillioides MRC 826 as the source of FB(1). The amount of FB(1) detected in the masa and tortillas decreased as the concentration of Ca(OH)(2) increased, and boiling time had no apparent effect. Unexpectedly, as the initial concentrations were increased in the corn prior to nixtamalization, greater percentage reductions in FB(1) were observed.  相似文献   

FACULTY POSITION ANNOUNCEMENT     

Joan C. Hendricks  VMD  PhD 《Veterinary radiology & ultrasound》2003,44(1):125-127

  相似文献   

Tracheal collapse in two cats   总被引：1，自引：0，他引：1  

J C Hendricks  J A O'Brien 《Journal of the American Veterinary Medical Association》1985,187(4):418-419

Two cats examined bronchoscopically to discover the cause of tracheal collapse were found to have tracheal obstruction cranial to the collapse. Cats with this unusual sign should be examined bronchoscopically to ascertain whether there is an obstruction, as the cause in these 2 cats was distinct from the diffuse airway abnormality that causes tracheal collapse in dogs.  相似文献   

To the Editor of "Science:"     

Hendricks JE 《Science (New York, N.Y.)》1881,2(69):496

  相似文献   

Juvenile-onset and adult-onset demodicosis in dogs in the UK: prevalence and breed associations     

D. G. O'Neill  E. Turgoose  D. B. Church  D. C. Brodbelt  A. Hendricks 《The Journal of small animal practice》2020,61(1):32-41

  相似文献   

"Big" growth hormone components from human plasma: decreased reactivity demonstrated by radioreceptor assay   总被引：1，自引：0，他引：1  

P Gorden  M A Lesniak  C M Hendricks  J Roth 《Science (New York, N.Y.)》1973,182(114):829-831

Plasma as well as pituitary immunoreactive human growth hormone (HGH) comprises at least two discrete components which have been designated as "big" HGH and "little" HGH. Using a newly developed radioreceptor assay, which depends on the ability of a substance to compete with labeled HGH for binding sites on cultured human lymphocytes, we find that the big HGH component from both normal and acromegalic subjects has much less activity in the radioreceptor assay than in the radioimmunoassay, whereas the little HGH component has simnilar activity in both assays.  相似文献   

FITTING THE CURVE OF THE DIMINISHING INCREMENT TO FEED CONSUMPTION-LIVE WEIGHT GROWTH CURVES     

Hendricks WA 《Science (New York, N.Y.)》1931,74(1916):290-291

  相似文献   

THE INORGANIC CONSTITUTION OF BONE     

Hendricks SB  Hill WL 《Science (New York, N.Y.)》1942,96(2489):255-257

  相似文献