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131.
Outbreaks of Salmonella Dublin infections were recorded in 25 Danish mink and fox farms. All farms suffered extensive disease problems; clinical and pathological observations included abortion, stillbirths, necrotizing endometritis, and increased mortality. By genotyping with pulsed-field gel electrophoresis and amplified fragment length polymorphism, all isolates of S. Dublin had indistinguishable patterns. The outbreaks took place during April and May, around the time of whelping. During this period, mink are particularly susceptible to Salmonella infections. All affected farms were served by the same feed factory and it was concluded that a batch of contaminated feed was responsible for the outbreaks, although repeated culture of feed samples collected during the same period were negative. No other likely source could be identified. The results emphasize the importance of strict hygiene measures at feed factories and the proper use of ingredients of known Salmonella status, in particular during the whelping season. Infected mink farms did not have a higher risk of outbreak of salmonellosis in the year following the outbreak.  相似文献   
132.
133.
In this study, we quantified the effects of local neighbourhood competition, light availability, and proximity to skid trails on the growth of sugar maple (Acer saccharum Marsh.) trees following selection harvest. We hypothesized that growth would increase with decreasing competition and increasing light availability, but that proximity to skid trails would negatively affect growth. A total of 300 sugar maples were sampled ∼10 years after selection harvesting in 18 stands in Témiscamingue (Québec, Canada). Detailed tree and skid trail maps were obtained in one 0.4 ha plot per stand. Square-root transformed radial growth data were fitted to a linear mixed model that included tree diameter, crown position, a neighbourhood competition index, light availability (estimated using the SORTIE light model), and distance to the nearest skid trail as explanatory variables. We considered various distance-dependent or -independent indices based on neighbourhood radii ranging from 6 to 12 m. The competition index that provided the best fit to the data was a distance-dependent index computed in a 6 m search radius, but a distance-independent version of the competition index provided an almost equivalent fit to data. Models corresponding to all combinations of main effects were fit to data using maximum likelihood, and weighted averages of parameter estimates were obtained using multimodel inference. All predictors had an influence on growth, with the exception of light. Radial growth decreased with increasing tree diameter, level of competition and proximity to skid trails, and varied among crown positions with trees in suppressed and intermediate positions having lower growth rates than codominants and dominants. Our results indicate that in selection managed stands, the radial growth of sugar maple trees depends on competition from close (≤6 m) conspecific neighbours, and is still affected by proximity to skid trails ∼10 years after harvesting. Such results underscore the importance of minimizing the extent of skid trail networks by careful pre-harvest planning of trail layout. We also conclude that the impact of heterogeneity among individual-tree neighbourhoods, such as those resulting from alternative spatial patterns of harvest, can usefully be integrated into models of post-harvest tree growth.  相似文献   
134.
Responsive fertilisation of winter wheat (Triticum aestivum L.) is often adopted, with N applied two or three times between the developmental stages of tillering and booting. Satellite-based decision support systems (DSS) providing vegetation index maps calculated from satellite data are available to aid farmers in adjusting the topdressing nitrogen (N) rate site-specifically to the current season and to variations in growth conditions within the field. One example is the freely available CropSAT DSS used in Scandinavia, which provides farmers with raster maps of the modified soil-adjusted vegetation index (MSAVI2) calculated mainly from data obtained from satellites Sentinel-2 (ESA, EU) and DMC (DMCii Ltd, Guildford, UK). This study investigated the possibility of calibrating MSAVI2 maps with data from handheld proximal sensor measurements of N uptake covering the main agricultural regions in Sweden during growth stages Z30-45 on the Zadok scale, in order to facilitate farmers’ decisions on N rate. More than 200 N-sensor measurements acquired during 2015 and 2016 in seven different winter wheat cultivars were combined with MSAVI2 values from CropSAT. It was found that N uptake could be predicted in a general, national model, i.e. for sites and dates other than those for which the calibration model was parameterised, with a mean absolute error of 11–15?kg?N?ha?1. A cultivar-specific model performed better than this general model, but a regional model showed no improvement compared with the model parameterised with national data. Vegetation indices calculated from the two narrow bands of Sentinel-2 in the red edge-near infrared region of the crop canopy reflectance spectrum proved to be promising alternatives to the broadband index MSAVI2. Based on the results, we suggest that data from a monitoring programme involving handheld N sensor measurements can be integrated with a satellite-based DSS to upscale N uptake information.  相似文献   
135.
Wheat bread and whole wheat bread were stored for up to 3 weeks and specific chemical reactions were related to perceptual flavour changes as analysed by sensory profiling. Volatile and non-volatile compounds were quantified using GC–MS and HPLC. Results were examined by multivariate data analysis.  相似文献   
136.
In 2001, the first case of Postweaning Multisystemic Wasting Syndrome (PMWS) was reported in the Danish pig population. During subsequent years, the number of affected farms increased exponentially. The aim of this study was to determine how this increase influenced the use of antimicrobials between 2002 and 2004. We used national register data of herd characteristics, antimicrobial usage and disease occurrence. The analysis included data on antimicrobial usage in 3371 pig herds with weaners and 7434 pig herds with finishers, which accounted for 56 and 82% of the national amount of antimicrobials prescribed to weaners (prescribed by 347 practitioners) and finishers (prescribed by 522 practitioners), respectively.The estimation of the effect of PMWS was done by comparing the amount of antimicrobials (measured as Animal Defined Daily Doses (ADDkg) used per pig-day at risk each month in each herd) used in herds before and after an outbreak of PMWS, and by comparing the amount of antimicrobials used in herds experiencing PMWS with the amount of antimicrobials used in herds not experiencing PMWS. The effects were estimated in a three-level (veterinarian/herd/study-month) linear mixed regression model with an autoregressive correlation of order 1 (AR1).We found that after a herd had experienced an outbreak of PMWS, the antimicrobial usage in weaners was increased for a year. During the first 3 months post outbreak the usage increased by 22%, followed by an increase of 7% during the next 4th to 12th month when compared to the pre-outbreak usage. There was a significant variation between herds in this effect. Additionally, in herds experiencing an outbreak of PMWS, the usage of antimicrobials before the outbreak was 37 and 19% higher in herds with weaners and finishers, respectively, compared to herds not experiencing PMWS.Generalisation of the results to the entire Danish pig population indicated that the increase of PMWS infected herds from almost zero to about 20% during a 4-year period resulted in a national increase of 4–5% in antimicrobials usage in weaners. The effect of PMWS on usage of antimicrobials in finishers was unclear.  相似文献   
137.
The objective of this study was to compare results from endometrial cytological and bacteriological examinations obtained by two different laboratories. One laboratory used endometrial swabs and the other endometrial biopsies for the examinations. A higher proportion of sterile, cytology positive cases were found in the laboratory using swabs (148/401 [37%] vs 12/237 [5%], P < .0001). In both laboratories it was found that positive cytology was less likely to occur when Escherichia coli was isolated than if other species were isolated. This tendency was significant in both laboratories (P < .05 vs P < .0001).  相似文献   
138.
We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel–dependent nerve conductance in male Wistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and death likely emanate from a compound <5 kDa, and via a different mechanism of action than that of TTX. The similarity in mass between TTX and the Vibrio-produced low-molecular-weight, non-toxic compound invokes that thorough analysis is required when assessing TTX production. Based on our findings, we suggest that re-examination of some published claims of TTX production may be warranted.  相似文献   
139.
  • Dredging blue mussels (Mytilus edulis) and thus removing structural elements, inducing resuspension of sediment as well as reducing filtration capacity, will inevitably affect the ecosystem. The study demonstrates that the impacts of fishing can be reduced through gear developments.
  • A new light dredge was tested on commercial vessels using two different experimental setups. First, a twin haul experiment tested the standard gear (i.e., a Dutch dredge) against the light dredge by fishing the two gears side by side onboard the same vessel. Second, a single dredge experiment tested the absolute performance of the two gears by fishing in areas with a known blue mussel density.
  • Results from the twin haul experiment demonstrate that the weight of sediment retained in the gear per square metre fished is 49% less in the light dredge compared with the Dutch dredge which will reduce resuspension of sediment at the surface. Also, the drag resistance of the light dredge was significantly less (177.1 vs. 202.7 kg m‐1). In the twin haul experiment no significant difference was found in the catch per unit effort (CPUE) of the two gears. The single dredge experiment, on the other hand, demonstrated a significant increase in CPUE exceeding 200% when using the light dredge.
  • Seafloor tracks made by the two dredges could not be distinguished by use of side‐scan sonar and the tracks were still detectable 2 months after fishing.
  • It was concluded that replacement of the Dutch dredge with the light dredge would reduce the impact of the fishery on the ecosystem by (i) reducing resuspension of sediment, (ii) reducing fuel consumption, and (iii) potentially reducing energy transfer to the sediment through a reduced gear drag resistance. A potential increase in catch efficiency may reduce the area affected.
  • Fishing with the light dredge is discussed in relation to management of Natura 2000 sites.
Copyright © 2014 John Wiley & Sons, Ltd.  相似文献   
140.
Urban paved sites are complex stress environments and when selecting trees for such sites, tree planners must prioritise stress tolerance above aesthetic appeal and functional aspects. This requires detailed information about the tolerance of tree species to environmental stresses, so as to support urban tree planners in selecting a wide range of trees. In Scandinavia there is currently no overview of the extent to which the character of information about stress tolerance and its dissemination in books and papers support urban tree planners in selecting a wide range of species for paved sites. Books on dendrology, literature on plant use in cities, tree nursery catalogues and scientific papers were therefore reviewed. For the review, eight tree species were selected along a gradient representing their intensity of use in Northern Europe. We examined the character of information and assessed it relative to tree planners’ requirements for information to be; contextual, local to Scandinavia, referring to existing plantings in paved sites and recommendations for use of the species in paved sites. The results showed that existing information is piecemeal and that most is either too general (dendrology literature) or too specific or contradictory (scientific literature) to meet the requirements of urban tree planners, while books intended for plant use in cities do not sufficiently integrate the local perspective. Moreover, contextual information local to the Scandinavian region is mainly provided for already much used species. These findings led to suggestions on how future urban forestry and arboriculture research and dissemination efforts in Scandinavia can encourage tree planners to use a greater variety of tree species in urban paved sites.  相似文献   
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