Selective inhibition of glycoprotein and membrane protein of vesicular stomatitis virus from interferon-treated cells   总被引：2，自引：0，他引：2  

R K Maheshwari  F T Jay  R M Friedman 《Science (New York, N.Y.)》1980,207(4430):540-541

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Tunicamycin enhances the antiviral and anticellular activity of interferon   总被引：1，自引：0，他引：1  

R K Maheshwari  T Sreevalsan  R H Silverman  J Hay  R M Friedman 《Science (New York, N.Y.)》1983,219(4590):1339-1341

The inhibitory effects of interferon on virus multiplication and cell growth are significantly enhanced by treatment with tunicamycin. Potentiation of antiviral activity was found only with enveloped viruses and not with nonbudding viruses. Changes in the plasma membrane of treated cells may account for this effect, since enveloped viruses bud from the cell surface as a terminal step.  相似文献   

Dispersible exfoliated zeolite nanosheets and their application as a selective membrane     

Varoon K  Zhang X  Elyassi B  Brewer DD  Gettel M  Kumar S  Lee JA  Maheshwari S  Mittal A  Sung CY  Cococcioni M  Francis LF  McCormick AV  Mkhoyan KA  Tsapatsis M 《Science (New York, N.Y.)》2011,334(6052):72-75

Thin zeolite films are attractive for a wide range of applications, including molecular sieve membranes, catalytic membrane reactors, permeation barriers, and low-dielectric-constant materials. Synthesis of thin zeolite films using high-aspect-ratio zeolite nanosheets is desirable because of the packing and processing advantages of the nanosheets over isotropic zeolite nanoparticles. Attempts to obtain a dispersed suspension of zeolite nanosheets via exfoliation of their lamellar precursors have been hampered because of their structure deterioration and morphological damage (fragmentation, curling, and aggregation). We demonstrated the synthesis and structure determination of highly crystalline nanosheets of zeolite frameworks MWW and MFI. The purity and morphological integrity of these nanosheets allow them to pack well on porous supports, facilitating the fabrication of molecular sieve membranes.  相似文献   

Callusing and Regeneration of Plantlets via Somatic Embryogenesis from Inflorescence Cultures of Triticum aestivum L.: Role of Genotype and Long-Term Retention of Morphogenic Potential     

K. Rajylakshmi    S. K. Dhir    N. Maheshwari  S. C. Maheshwari 《Plant Breeding》1988,101(1):80-85

In the present investigation we have succeeded in obtaining a high frequency of regeneration of plantlets via somatic embryogenesis from callus derived from immature inflorescence explains of Triticum aestivum var, ‘Sonalika’. The explants were cultured on MS medium supplemented with 2,4-D, casein hydrolysate and coconut milk. A large number of embryoids germinated, to form plantlets on the medium when 2, 4-D was omitted altogether or provided at low concentration. Plantlets were transferred to soil under natural environmental conditions and were shown to have the normal chromosome number of 2n = 6x = 42. Experiments with nineteen other varieties show that there is a marked effect of genotype both on initiation of callusing as well as on regeneration. So far ‘Sonahka’ has proved to be the most responsive among varieties tested by us. With callus of the variety ‘Sonalika.’, we also conducted an investigation on long-term retention of regenerative potential. During Song-term culture, for about 12 months, the morphogenic potential gradually diminished and was finally lost, but the regeneration potential could be restored by subculturing at very short intervals.  相似文献   

Reduction in dose of chemical fertilizers and growth enhancement of sesame (Sesamum indicum L.) with application of rhizospheric competent Pseudomonas aeruginosa LES4     

Sandeep Kumar  Piyush Pandey  D.K. Maheshwari   《European Journal of Soil Biology》2009,45(4):334-340

Pseudomonas aeruginosa LES4, an isolate of tomato rhizosphere was found to be positive for several plant growth-promoting attributes like production of indole acetic acid, HCN and siderophore, solubilization of inorganic phosphate along with urease, chitinase and β-1-3-glucanase activity. In addition, it showed strong antagonistic effect against Macrophomina phaseolina and Fusarium oxysporum. P. aeruginosa LES4 caused halo cell formation and other morphological deformities in mycelia of M. phaseolina and F. oxysporum. Root colonization was studied with Tn5 induced streptomycin resistant transconjugants of spontaneous tetracycline-resistant LES4 (designated LES4^tetra+strep+) after different durations. The strain was significantly rhizospheric competent, as 17.4% increase in its population was recorded in sesame rhizosphere. Seed bacterization with LES4 resulted in significant increase in vegetative growth parameters and yield of sesame over non-bacterized seeds. However, application of LES4 with half dose of fertilizers resulted in growth equivalent to full dose treatment, without compromising with the growth and yield of sesame. Moreover, the oil yield increased by 33.3%, while protein yield increased by 47.5% with treatment of half dose of fertilizer along with LES 4 bacterized seeds, as compared to full dose of fertilizers.  相似文献   

Cartwheel architecture of Trichonympha basal body     

P Guichard  A Desfosses  A Maheshwari  V Hachet  C Dietrich  A Brune  T Ishikawa  C Sachse  P Gönczy 《Science (New York, N.Y.)》2012,337(6094):553

Centrioles and basal bodies are essential for the formation of cilia, flagella, and centrosomes. They exhibit a characteristic ninefold symmetry imparted by a cartwheel thought to contain rings of SAS-6 proteins. We used cryoelectron tomography to investigate the architecture of the exceptionally long cartwheel of the flagellate Trichonympha. We found that the cartwheel is a stack of central rings that exhibit a vertical periodicity of 8.5 nanometers and is able to accommodate nine SAS-6 homodimers. The spokes that emanate from two such rings associate into a layer, with a vertical periodicity of 17 nanometers on the cartwheel margin. Thus, by using the power of biodiversity, we unveiled the architecture of the cartwheel at the root of the ninefold symmetry of centrioles and basal bodies.  相似文献   

Antimicrobial and pesticidal activity of partially purified flavonoids of Annona squamosa     

Kotkar HM  Mendki PS  Sadan SV  Jha SR  Upasani SM  Maheshwari VL 《Pest management science》2002,58(1):33-37

Foliar extracts of Annona squamosa (Family: Annonaceae) were screened for antimicrobial and insecticidal activity against the common microbial infestants of pulses and the stored grain pest pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae). Flavonoids isolated from aqueous extracts of A squamosa showed antimicrobial activity against all the common microbial contaminants of pulses and 80% insecticidal activity against C chinensis at a concentration of 0.07 mg ml-1. Various physico-chemical tests, chromatographic and spectroscopic studies with partially purified aqueous extract indicated the presence of flavonol type flavonoids. This may provide a useful beginning for the development of botanical pesticides for post-harvest safeguard of pulses.  相似文献   

Cryopreservation of turbot Scophthalmus maximus (L.) sperm: fertilization and hatching rates     

O Chereguini  I García de la Banda  M Herrera  C Martinez  & M De la Hera 《Aquaculture Research》2003,34(9):739-747

The present paper assesses the fertilization and hatching rates of an artificial fertilization series (n=1153) using fresh and cryopreserved sperm from 49 specimens of turbot Scophthalmus maximus (L.), carried out to confirm the results of a previous study, with the ultimate aim of transferring these cryo‐preservation techniques to commercial hatcheries. No significant differences were found between the fertilization rates of the two groups (fresh and cryopreserved sperm) when their respective fertility rates were >69.2%, which was the case in 75% of all fertilizations. Likewise, no significant differences in hatching rates were found. In order to use the cryopreserved sperm more efficiently, a key concern for commercial use of this technique, we also experimented with a lower sperm:diluent ratio (1:1) than used previously at our centre. We also compared the traditional 0.5‐mL straws with 2‐mL cryotubes able to contain a higher volume of sperm, finding no significant differences in the resulting fertilization and hatching rates. In conclusion, the use of cryopreservation for turbot sperm presents major advantages for broodstock management in commercial hatcheries.  相似文献   

Generalized severe junctional epidermolysis bullosa with congenital absence of skin in churra lambs          下载免费PDF全文

Julio Benavides  Aroa Suárez‐Vega  Jorge Gutiérrez  Sandra de la Hera  Miguel Fuertes  Laetitia Delgado  Miguel Fernández  María del Carmen Ferreras  Juan José Arranz  Valentín Pérez 《Veterinary dermatology》2015,26(5):367-e83

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Optimal storage temperature and 1-MCP treatment combinations for different marketing times of Korla Xiang pears     

Xiao-hui JIA  Wen-hui WANG  Yan-min DU  Wei TONG  Zhi-hua WANG  Hera Gul 《农业科学学报》2018,17(3):693-703

Maintenance of green color is the primary indicator of quality in the market evaluation of Korla Xiang pears at present and can generally be achieved through early harvesting and decreasing the storage temperature, but the fruit quality was reduced by early harvesting, and the decreasing storage temperature increased the risk of chilling injury. The objectives of this study were to determine the optimal storage parameters for different storage times and to find ways to preserve the green skin color of pears. Specifically, we analyzed the effects of the ethylene inhibitor, 1-methylcyclopropene(1-MCP), combined with low temperature on quality and maintenance of the green color of Korla Xiang pears during storage. We found that 1-MCP and/or low temperature reduced the loss of green color at 20°C after being removed from cold storage. In addition, 1-MCP significantly inhibited the decline of titratable acid and ascorbic acid but had no significant effect on fruit firmness and total soluble solids. Low temperature with or without 1-MCP inhibited the release of ethylene, inhibited the decline in the stalk preservation index, inhibited the increase in decay rate and weight loss rate during storage, and inhibited the increase in the core browning index after 225 days of storage. Different storage temperatures had different effects on the quality of Korla Xiang pears. Despite inhibiting ethylene release, a storage temperature of –1.5°C increased the respiration rate. Storage at –1.5°C caused core browning early during storage due to chilling injury, whereas at 2°C core browning occurred late during storage due to senescence. In late storage, 1-MCP had no significant effect on the maintenance of Korla Xiang pear quality at 2°C. Based on these results, we determined the optimal combinations of low temperature and 1-MCP treatment to maintain pear quality while avoiding chilling injury. For different marketing times, the optimal conditions for storage until New Year's Day(a storage duration of 90 days) are 2°C or 1-MCP combined with 2°C. For storage until the Spring Festival(a storage duration of 150 days), the optimal conditions are 0°C or 1-MCP combined with 0°C, and for storage until May(a storage duration of 225 days), the best conditions are 1-MCP combined with –1.5°C.  相似文献