A quantitative study on arginine catabolism by mixed ruminal bacteria,protozoa and their mixture in vitro     

Halima SULTANA  Hazizul HUSSAIN‐YUSUF  Toshihiro TAKAHASHI  Tetsuo MORITA  Ryoji ONODERA 《Animal Science Journal》2003,74(1):11-16

The catabolism of arginine (Arg) by mixed rumen bacteria (B), mixed rumen protozoa (P), and their mixture (BP) was quantitatively investigated in an in vitro system in order to confirm the metabolic pathway of Arg and provide basic information for enzymatic and molecular studies as well as an understanding of the quantitative distribution of metabolites. Rumen microbial suspensions (B, P, and BP) collected from fistulated goats were anaerobically incubated with or without 1 mmol/L Arg at 39°C for 12 h. Arg and other related compounds such as citrulline (Cit), ornithine (Orn), proline (Pro) and 5‐aminovaleric acid (5AV) in both supernatant and hydrolyzates of B, P, and BP suspensions were analyzed by HPLC. The metabolic pathways of Arg in mixed rumen bacteria and mixed rumen protozoa were considered to be as follows: rumen bacteria, Arg → Cit → Orn → Pro → 5AV → VFAs + NH₃; rumen protozoa, Arg → Cit → Orn → Pro → 5AV. The disappearance of Arg (1 mmol/L) was approximately 52.9 and 88.2% in B, 33.9 and 55.6% in P, and 52.8 and 85.2% in BP during 6 and 12 h incubations, respectively. When expressed in units of ‘per gram (g) of microbial nitrogen (MN)’, the net degradation rate of Arg in BP (50.3 µmol/g MN/h) was approximately 46% lower than that of B during a 12 h incubation period. The presence of protozoa tended to inhibit the production of Orn from Cit and the production of 5AV from Pro which were thought to be rate‐limiting steps of Arg metabolism in rumen microorganisms. As a result, protozoa appeared to have a saving effect on Arg metabolism, that is, protozoa protected Arg from wasteful exhaustion in the rumen.  相似文献   

Enzyme sensor system for determination of total cholesterol in fish plasma     

Hideaki  ENDO  Masashi  MAITA  Mio  TAKIKAWA  Huifeng  REN  Tetsuhito  HAYASHI  Naoto  URANO  Kohji  MITSUBAYASHI 《Fisheries Science》2003,69(6):1194-1199

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Annual changes in the hatching period of the dominant cohort of larval and juvenile ayu Plecoglossus altivelis altivelis in the Shimanto Estuary and adjacent coastal waters during 1986–2001     

ISAO  TAKAHASHI  KENSAKU  AZUMA  SHINJI  FUJITA  IZUMI  KINOSHITA  HIROYUKI  HIRAGA 《Fisheries Science》2003,69(3):438-444

ABSTRACT:   Larval and juvenile ayu from seven year-groups (1986, 1987, 1992, 1995, 1996, 1999 and 2000) were collected in the Shimanto Estuary and adjacent coastal waters. The present study focuses on the variation among year-groups in the hatching period estimated by age determination using the otolith. The hatching period of the dominant cohorts in the 1986, 1987 and 1992 year-groups occurred from late October to mid-November. A delay of the hatching period of the dominant cohort was observed from the 1995 year-group, which occurred in late November to early December, and the dominant periods in the 1996, 1999 and 2000 year-groups were observed from early to late December. It appears that the delay of the hatching period of dominant cohorts was not due to a delay of spawning, but a high mortality of early hatched larvae. The water temperature in autumn in the coastal waters adjacent to the Shimanto River has tended to rise over the past 20 years and this trend was especially notable in the late 1990s. The delay in the hatching period of the dominant cohort observed from the 1995 year-group was likely to be related to the rise in seawater temperatures in autumn.  相似文献   

Cytotoxic constituents in the holdfast of cultivated Laminaria japonica     

Makoto  NISHIZAWA  Nobuaki  TAKAHASHI  Kumiko  SHIMOZAWA  Takako  AOYAMA  Kouichi  JINBOW  Yukari  NOGUCHI  Kiyoshi  HORITA  Hideo  BANDO  Takashi  YAMAGISHI 《Fisheries Science》2003,69(3):639-643

ABSTRACT:     Four steroidal ketones were isolated from the holdfast of cultivated Laminaria japonica . The structures were characterized as ergosta-4,24(28)-diene-3-one (1), ergosta-4,24(28)-diene-3,6-dione (2), stigmasta-4,24(28)-diene-3-one (3) and stigmasta-4,24(28)-diene-3,6-dione (4), by spectral data. Compounds 2 and 4 were shown to be cytotoxic against the MCF-7 human breast cancer cell, and the growth of MCF-7 was inhibited by 96% and 79%, respectively, at 10 µg/mL. It is the first report on the isolation of cytotoxic steroidal ketones from the kelp in the genus of Laminaria.  相似文献   

Quantitative real‐time PCR monitoring of Escherichia coli and Clostridium perfringens with oral administration of Lactobacillus plantarum strain Lq80 to weaning piglets     

Shunsuke TAKAHASHI  Yoko YOSHIDA  Nobuo NAKANISHI  Takamitsu TSUKAHARA  Kazunari USHIDA 《Animal Science Journal》2008,79(6):737-744

Levels of fecal or intestinal lactobacilli, Escherichia coli and Clostridium perfringens, and the prevalence of clostridial alpha toxin gene and heat‐stable toxin (ST) gene of enterotoxigenic E. coli (ETEC) were monitored in weaned piglets before (day 0) and during (days 7, 14, and 21) the administration of Lactobacillus plantarum strain Lq80. Lactobacilli were enumerated in a culture‐dependent method. The remainders were determined by quantitative real‐time PCR. In this quantitative real‐time PCR method, the detection limit was proved to be as low as 10³ cells/g feces or intestinal contents. Number of lactobacilli increased from day 0 to day 7 (P < 0.05), to day 14 (P < 0.05), and to day 21 (P = 0.07) in the Lq80‐administered group. L. plantarum contributed to as low as 10% of the lactobacillal population in the Lq80‐administered group. The number of E. coli and C. perfringens, and the prevalence of alpha toxin gene in feces or intestinal contents of the Lq80‐administered group decreased, at least in the first week of the postweaning period. Oral administration of L. plantarum strain Lq80 can stimulate the growth of indigenous lactobacilli and decrease ST‐producing ETEC and C. perfringens in the intestine of postweaning piglets.  相似文献   

Complete Nucleotide Sequence of Ryegrass Mottle Virus : A New Species of the Genus Sobemovirus     

Feiyun ZHANG  Shigemitsu TORIYAMA  Mami TAKAHASHI 《Journal of General Plant Pathology》2001,67(1):63-68

The genome of Ryegrass mottle virus (RGMoV) comprises 4210 nucleotides. The genomic RNA contains four open reading frames (ORFs). The largest ORF 2 encodes a polyprotein of 947 amino acids (103.6 kDa), which codes for a serine protease and an RNA-dependent RNA polymerase. The viral coat protein is encoded on ORF 4 present at the 3′-proximal region. Other ORFs 1 and 3 encode the predicted 14.6 kDa and 19.8 kDa proteins of unknown function. The consensus signal for frameshifting, heptanucleotide UUUAAAC and a stem-loop structure just downstream is in front of the AUG codon of ORF 3. Analysis of the in vitro translation products of RGMoV RNA suggests that the 68 kDa protein may represent a fusion protein of ORF 2-ORF 3 produced by frameshifting. The protease region of the polyprotein and coat protein have a low similarity with that of the sobemoviruses (approximately 25% amino acid identity), while the RNA-dependent RNA polymerase region has particularly strong similarity (54 to 60% of more than 350 amino acid residues). The sequence similarities of RGMoV to the sobemoviruses, together with the characteristic genome organization indicate that RGMoV is a new species of the genus Sobemovirus. Received 28 June 2000/ Accepted in revised form 14 November 2000  相似文献   

Software development for estimating the concentration of radioactive cesium in the skeletal muscles of cattle from blood samples          下载免费PDF全文

Tomokazu Fukuda  Masahiro Hiji  Yasushi Kino  Yasuyuki Abe  Hideaki Yamashiro  Jin Kobayashi  Yoshinaka Shimizu  Atsushi Takahashi  Toshihiko Suzuki  Mirei Chiba  Kazuya Inoue  Yoshikazu Kuwahara  Motoko Morimoto  Masafumi Katayama  Kenichiro Donai  Hisashi Shinoda  Tsutomu Sekine  Manabu Fukumoto  Emiko Isogai 《Animal Science Journal》2016,87(6):842-847

The 2011 earthquake severely damaged the Fukushima Daiichi Nuclear Power Plant (FNPP), resulting in the release of large quantities of radioactive material into the environment. The deposition of these radionuclides in rice straw as livestock feed led to the circulation of contaminated beef in the market. Based on the safety concern of the consumers, a reliable method for estimating concentrations of radioactive cesium in muscle tissue is needed. In this study, we analyzed the concentrations of radioactive cesium in the blood and skeletal muscle of 88 cattle, and detected a linear correlation between them. We then developed software that can be used to estimate radioactive cesium concentrations in muscle tissue from blood samples. Distribution of this software to the livestock production field would allow us to easily identify high‐risk cattle, which would be beyond the safety regulation, before shipping out to the market. This software is planned to be released as freeware. This software would contribute to food safety, and aid the recovery of the livestock industry from the damage creacted by the 2011 Tohoku earthquake and tsunami.  相似文献   

Practical Application of Miyazaki Jitokko Chickens Selected for a Superior Allele at a Single Nucleotide Polymorphism Site in the Cholecystokinin Type A Receptor Gene     

Shojiroh Horinouchi  Hiromi Nakayama  Tadahiro Ando  Hideaki Takahashi 《The Journal of Poultry Science》2021,58(1):12

This study aimed to examine 1) whether selection for a superior allele at a single nucleotide polymorphism site (SNP; {"type":"entrez-nucleotide","attrs":{"text":"AB604331","term_id":"315258605","term_text":"AB604331"}}AB604331, g.420 C>A) of the chicken cholecystokinin type A receptor (CCKAR) gene in Miyazaki Jitokko chickens is detectable in commercial poultry farms, and 2) whether the reproductive traits of the Kyushu Rhode hens, as a maternal stock line of the Miyazaki Jitokko chickens, are affected by SNP selection. Conventional and A-allele fixed (improved) Miyazaki Jitokko chicks were hatched on the same day and raised in a battery cage until 7 days of age. The chicks were then deposited at two commercial poultry farms and reared until slaughter at 126 and 163 days for cockerels and pullets, respectively. Body weight on the day of hatching (day 0), at 5 days of age, and at slaughter were measured. The differences in the body weights of the farm and test groups at slaughter were analyzed using the generalized linear model. A-allele fixation increased the body weight at slaughter by approximately +123.5 g and +131.9 g in cockerels and pullets, respectively. No significant differences between the conventional and improved hens were detected in terms of egg-laying rate, fertilization rate, and hatchability in the Kyushu Rhode hens. The data suggest that fattening chicks can be supplied as usual, even if Kyushu Rhode hens are switched from the conventional to improved type. In conclusion, genetic improvements using the CCKAR SNP site as a marker were effectively established in terms of the growth of the Miyazaki Jitokko chickens in commercial farms and the reproductive traits of the Kyushu Rhode hens.  相似文献   

Effect of feeding non-fibrous carbohydrate before grazing on intake and nitrogen utilization in dairy cows throughout the grazing season     

Tomohiro MITANI  Koichiro UEDA  Tetsushiro ENDO  Makoto TAKAHASHI  Hiroki NAKATSUJI  Seiji KONDO 《Animal Science Journal》2012,83(2):121-127

Ten lactating cows were used to determine the effect of feeding non-fibrous carbohydrate (NFC) supplement before grazing on feed intake and nitrogen (N) utilization throughout a grazing season. The experiment was conducted from June to September. Cows grazed twice a day (2.5 h × 2) under a set stocking system and were fed NFC supplement (1 kg/4 kg of milk yield) 2 h before grazing (PRE) or immediately after grazing (POST). Cows were also fed a grass and corn silage mixture ad libitum. Herbage dry matter intake (DMI) was greater for PRE than for POST throughout the experiment and decreased from June to September. Conversely, silage DMI was less for PRE than for POST throughout the experiment and increased over the grazing season. Consequently, total DMI for PRE did not differ from that for POST. Milk urea-N concentration and urinary urea-N excretion in June did not differ between the treatments, whereas that after July was higher for PRE than for POST. Proportion of urinary N excretion to absorbed N intake in June was lower for PRE than for POST, but that after July was higher for PRE than for POST. Feeding NFC supplement before grazing would improve N utilization when cows eat large amounts of herbage high in N.  相似文献   

Fire resistance of thick wood-based boards   总被引：1，自引：0，他引：1  

Toshiro Harada  Saburou Uesugi  Hideaki Masuda 《Journal of Wood Science》2006,52(6):544-551

Thick wood-based boards are used as construction materials for walls and floors in Japan. In this study, fire resistance tests (ISO 834-1) and cone calorimeter tests (ISO 5660-1) were conducted for thick plywood, particleboard, and medium density fiberboard with sample thicknesses of about 28–30mm, and their suitabilities for quasi-fireproof or fire-preventive structures were evaluated. In the ISO 834-1 fire resistance test, the heat-shielding performance (insulation criterion) for walls was evaluated and the results showed that the larger the apparent density of a woodbased board, the higher its insulation performance. The insulation performance of thick wood-based boards in the fire resistance test could be forecast from the results of the cone calorimeter test, especially when the second peak of heat release rate appeared. In the cone calorimeter tests, the surface layer density of the plywood, particleboard, and medium density fiberboard was the dominant parameter for the time to ignition and initial heat release rate. These results indicate that thick wood-based board is a suitable fire-preventive construction material. Part of this study was presented at the Annual Meeting of the Architectural Institute of Japan, Hokkaido, Japan, August 2004  相似文献