Ranavirus in an outbreak of dermatophilosis in captive inland bearded dragons (Pogona vitticeps)          下载免费PDF全文

Kenichi Tamukai  Toshihiro Tokiwa  Hideki Kobayashi  Yumi Une 《Veterinary dermatology》2016,27(2):99-e28

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Characteristics of Collagen from Rohu (Labeo rohita) Skin     

Wanticha Savedboworn  Soottawat Benjakul  Sittichoke Sinthusamran  Hideki Kishimura 《Journal Of Aquatic Food Product Technology》2017,26(3):248-257

Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) were isolated from rohu skin with the yield of 64.2 and 6.8% (dry weight basis), respectively. Both collagens had glycine as the major amino acid with imino acid content of 196–202 residues/1,000 residues and were characterized as type I collagen with molecular composition of (α1)₂α2-heterotrimer. Fourier transform infrared spectra of both collagens were similar, with no shift in wavenumber of all amide bands. The T_max value of ASC and PSC was 36.40 and 35.48°C, respectively. The zero surface net charge of ASC and PSC was found at pH 5.9 and 5.3, respectively.  相似文献   

Characteristics and Functional Properties of Ovary from Squid Loligo Formosana     

Avtar Singh  Hideki Kishimura 《Journal Of Aquatic Food Product Technology》2017,26(9):1083-1092

The characteristics and functional properties of the ovary from Loligo formosana were studied. Moisture (72.07 ± 0.24%) was dominant, followed by protein (18.64 ± 0.51%) and carbohydrate (7.44 ± 0.2%). Ash (1.39 ± 0.03%) and lipids (0.46 ± 0.5%) were found as the minor constituents. Albumin (79.02 ± 0.79%) was the major protein of the squid ovary, followed by glutelin-1 (8.31 ± 0.62%) and globulin (6.68 ± 0.08%). Nevertheless, prolamin and glutelin-2 constituted approximately 1% of the total proteins. Based on the electrophoretic studies, albumin had the largest band intensity. The squid ovary was rich in non-essential amino acids (52.26%) and high in hydrophobic amino acids (48.03%). It was also rich in polyunsaturated fatty acid (PUFA, 43.76 ± 0.84%), followed by saturated fatty acid (SFA, 39.36 ± 0.12%) and monounsaturated fatty acid (MUFA, 12.94 ± 0.55%). Ovary lipids had a high amount of docosahexaenoic acid (C22:6) (28.59%). At pH 3, the squid ovary powder (SOP) had the maximum solubility (96.39%), whereas the lowest solubility (38.33%) was observed at pH 9. The foaming capacity and stability of SOP were increased with increasing concentration up to 8% (p < 0.05). The globulin fraction showed the higher foaming capacity, as compared to albumin and glutelin-1 fractions. The squid ovary had good nutritional value and possessed excellent foaming properties. Therefore, the squid ovary could serve as a novel food additive or ingredient.  相似文献   

Vitamin contents of eggs that produce larvae showing a high survival rate in the Japanese eel Anguilla japonica     

Hirofumi Furuita  Tatsuya Unuma  Kazuharu Nomura  Hideki Tanaka  Tsuyoshi Sugita  & Takeshi Yamamoto 《Aquaculture Research》2009,40(11):1270-1278

This paper describes the relationship between the egg vitamin concentrations and the egg quality in the Japanese eel Anguilla japonica . No notable relation was found between any vitamin and the fertilization rate. Hatching and survival rates of larvae, however, significantly increased with an elevated level of egg vitamin C (VC). In contrast to VC, the relation between vitamins E (VE) and A (VA) concentrations and survival rate showed a clear peak, with a reduced survival rate at both higher and lower vitamin concentrations. The ratio of VE to lipid or highly unsaturated fatty acid (HUFA) in eggs positively correlated with hatching and survival rates of larvae. High-quality (HQ) eggs were determined as eggs that produced larvae having a survival rate higher than 80% at 8 days post hatch, and low-quality (LQ) eggs were determined as eggs that did not hatch. The level of VC of HQ was significantly higher than LQ. The results of this study suggest that HQ eggs, which produce larvae having a high survival rate, must have high levels of VC and VE/HUFA ratio and contain optimum levels of VA and VE in Japanese eel.  相似文献   

Availability of potato protein concentrate as an alternative protein source to fish meal in greater amberjack (Seriola dumerili) diets     

Fumiaki Takakuwa  Keinosuke Suzuri  Takao Horikawa  Kunpei Nagahashi  Shinichi Yamada  Amal Biswas  Hideki Tanaka 《Aquaculture Research》2020,51(3):1293-1302

Potato protein concentrate (PPC) is a promising candidate as a fish meal (FM) substitute because it has high protein and essential amino acid content. In the present study, we replaced FM in greater amberjack diets with PPC to investigate the effect on growth and feed utilization. Four isonitrogenous, isolipidic and isocaloric experimental diets were prepared by substituting 0, 20, 40 and 60% of FM protein with PPC (Control, P20, P40 and P60 respectively). The in vitro protein digestibility of protein in PPC was 88.8%, relative to 100% protein in the FM. The in vitro protein digestibility of protein in the experimental diets also decreased with increasing PPC and was lowest at 84.2% in P60. After the 7‐week feeding trial, final body weight, weight gain and thermal growth coefficient tended to decrease with increasing PPC and were significantly lower in P60 than in the control (p < .05). Further, fish fed with diets P40 and P60 showed significantly lower feed conversion and protein efficiency ratios than the control group (p < .05). In conclusion, the results suggest that PPC can replace up to 20% of FM in the diet of greater amberjack without compromising the growth performance or feed efficiency.  相似文献   

Similarity and differences in the physicochemical properties of lactate dehydrogenase isozymes from different tissues of Japanese sandfish <Emphasis Type="Italic">Arctoscopus japonicus</Emphasis>     

Kotomi Sugawara  Mizuki Nakagawa  Mika Yonezawa  Shigeyoshi Nakamura  Shun-ichi Kidokoro  Hideki Wakui  Wataru Nunomura 《Fisheries Science》2016,82(3):519-527

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Structure-Activity Relationship Study of the Neuritogenic Potential of the Glycan of Starfish Ganglioside LLG-3     

Megumi Yamagishi  Ritsuko Hosoda-Yabe  Hideki Tamai  Miku Konishi  Akihiro Imamura  Hideharu Ishida  Tomio Yabe  Hiromune Ando  Makoto Kiso 《Marine drugs》2015,13(12):7250-7274

LLG-3 is a ganglioside isolated from the starfish Linchia laevigata. To clarify the structure-activity relationship of the glycan of LLG-3 toward rat pheochromocytoma PC12 cells in the presence of nerve growth factor, a series of mono- to tetrasaccharide glycan derivatives were chemically synthesized and evaluated in vitro. The methyl group at C8 of the terminal sialic acid residue was crucial for neuritogenic activity, and the terminal trisaccharide moiety was the minimum active motif. Furthermore, the trisaccharide also stimulated neuritogenesis in human neuroblastoma SH-SY5Y cells via mitogen-activated protein kinase (MAPK) signaling. Phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was rapidly induced by adding 1 or 10 nM of the trisaccharide. The ratio of phosphorylated ERK to ERK reached a maximum 5 min after stimulation, and then decreased gradually. However, the trisaccharide did not induce significant Akt phosphorylation. These effects were abolished by pretreatment with the MAPK inhibitor U0126, which inhibits enzymes MEK1 and MEK2. In addition, U0126 inhibited the phosphorylation of ERK 1/2 in response to the trisaccharide dose-dependently. Therefore, we concluded that the trisaccharide promotes neurite extension in SH-SY5Y cells via MAPK/ERK signaling, not Akt signaling.  相似文献   

Root and crown rot of strawberry caused by Pythium helicoides and its distribution in strawberry production areas of Japan     

Yasushi Ishiguro  Kayoko Otsubo  Hideki Watanabe  Mikihiko Suzuki  Kiichi Nakayama  Takashi Fukuda  Masashi Fujinaga  Haruhisa Suga  Koji Kageyama 《Journal of General Plant Pathology》2014,80(5):423-429

Pythium species were isolated from seedlings of strawberry with root and crown rot. The isolates were identified as P. helicoides on the basis of morphological characteristics and sequences of the ribosomal DNA internal transcribed spacer regions. In pathogenicity tests, the isolates caused root and crown rot similar to the original disease symptoms. Multiplex PCR was used to survey pathogen occurrence in strawberry production areas of Japan. Pythium helicoides was detected in 11 of 82 fields. The pathogen is distributed over six prefectures.  相似文献   

Characteristic upregulation of glucose-regulated protein 78 in an early lesion negative for hitherto established cytochemical markers in rat hepatocarcinogenesis     

Sukata T  Uwagawa S  Ozaki K  Sumida K  Kushida M  Kakehashi A  Wanibuchi H  Miyata K  Ogata K  Fukushima S 《Journal of toxicologic pathology》2009,22(4):281-288

Previously, we reported α(2)-macroglobulin (α(2)M) to be a novel marker characteristic of rat hepatocellular preneoplastic and neoplastic lesions negative for hitherto well-established markers. In the present study, we further examined other candidate markers with specificity for the same type of lesions. Glutathione S-transferase-placental form (GST-P)-negative hepatocellular altered foci (HAF) were generated using a two-stage (initiation and promotion) carcinogenesis protocol with N,N-diethylnitrosamine (DEN) and either Wy-14,643 or clofibrate, two peroxisome proliferators. Microarray analysis using total RNAs isolated from laser-microdissected GST-P-negative HAF (amphophilic cell foci) and adjacent normal tissues was conducted along with immunohistochemistry and real-time RT-PCR. Staining for glucose-regulated protein 78 (GRP78) was detected in GST-P-negative HAF and hepatocellular adenomas, and slightly increased GRP78 mRNA expression was observed in the lesions by real-time RT-PCR analysis. Thus, an early increase of GRP78 expression in hepatocarcinogenesis is likely a feature of the amphophilic subset of HAF.  相似文献   

Trophic structure in natural habitats of the abalone Haliotis discus hannai with distinct algal vegetation of kelp and crustose coralline algae: implication of ontogenetic niche shifts     

Nam-Il Won  Tomohiko Kawamura  Hideki Takami  Yoshiro Watanabe 《Fisheries Science》2013,79(1):87-97

The abundance, species composition, and stable isotope ratios of benthic organisms were investigated to determine the trophic structures in abalone (Haliotis discus hannai) habitats, which are characterized by contrasting vegetation of crustose coralline algae (CCA) and kelp beds. A size–frequency analysis revealed that juvenile abalones with shell lengths (SLs) smaller than ~30 mm primarily inhabited CCA beds, whereas adults were abundant in kelp beds. Stable isotope analyses indicated that CCA beds were composed of a single food chain, whereas kelp beds supported multiple food chains. The abalone were divided into three size groups to estimate potential species interactions during their ontogeny. A small gastropod, Homalopoma sangarense, was the most abundant species, but is suspected to be less competitive with abalone, especially in CCA beds. An abundant starfish Asterina pectinifera appeared to function as a potential predator of juvenile abalones in both CCA and kelp beds. We concluded that CCA beds are essential for immediate post-settlement processes of abalones, whereas kelp beds are more important for providing refuge and food sources for adult abalones. The present study highlights that ontogenetic niche shifting can be a successful life-history strategy to sustain the abalone population in a subtidal rocky shore ecosystem.  相似文献