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91.
Food colorants including rose bengal, erythrosine B, phloxine B and cochineal extracts were added to Alaska pollack Theragra chalcogramma surimi which was previously added with methyl linoleate (MeLe) or ethyl eicosapentaenoate (EPA Et). All the surimi were exposed
to a 100-watt light tungsten bulb (4500 lux) in a cold room at 5°C. Four isomeric hydroperoxides, including 13-cis, trans-, 13-trans, trans-, 9-trans,cis- and 9-trans,trans-MeLe hydroperoxides, were generated by oxidation of MeLe added to surimi with and without cochineal extracts after exposure
to light for several hours. For the surimi containing rose bengal, erythrosine B and phloxine B, extracted lipids contained
not only the above four hydroperoxide isomers but also 12-cis,trans-as well as 10-trans,cis- MeLe hydroperoxides. EPA Et added to surimi which contained rose bengal, erythrosine B and phloxine B generated characteristic
isomeric hydroperoxides, including 6-cis,trans- and 17-cis,trans-EPA Et hydroperoxides due to 1O2 mediated oxidation. The pro-oxidative potentials of four food colorants in surimi against added EPA Et or MeLe increased
as the order of cochineal extracts < phloxine B<erythrosine B<rose bengal. These results clearly demonstrated that the addition
of certain food colorants to surimi accelerated 1O2 mediated lipid oxidation to result in the generation of characteristic hydroperoxide isomers. 相似文献
92.
Yamada T Tochimaru N Nakasuji S Hata E Kobayashi H Eguchi M Kaneko J Kamio Y Kaidoh T Takeuchi S 《Veterinary microbiology》2005,110(1-2):97-103
Leukotoxin family genes in Staphylococcus aureus isolated from domestic animals were examined by polymerase chain reaction. LukS and lukF genes were detected in all 48 avian and 72 porcine isolates of S. aureus. LukE and lukD genes, located in a putative staphylococcal pathogenicity island (Sapln3/Saplm3), were recognized in 44 (91.7%) of 48 avian isolates, but these genes were not detected in porcine isolates. In 297 bovine isolates collected from mastitic cow's milk and bulk milk from dairy farms in two regions, lukM and lukF-PV(P83) genes in addition to lukS-lukF and lukE-lukD genes were detected in 100 (62.5%) of the 160 isolates from Ishikawa and in118 (86.1%) of the 137 isolates from Hokkaido. When the lysogeny of S. aureus bovine isolates was examined by treatment with mitomycin C, clearing of the culture due to cell lysis was observed in 34 (91.9%) of 37 lukM-lukF-PV(P83) genes--positive isolates. In addition, we isolated a novel lukM-lukF-PV(P83)-carrying (designated phiLukM), and revealed that the lukM-lukF-PV(P83) genes were located very close to an amidase gene on the temperate phage genomes. These results suggest horizontal transmission of lukM-lukF-PV(P83) genes by temperate bacteriophages in S. aureus of bovine origin. 相似文献
93.
Endo H Sasaki M Narushima E Komiya T Hayashida A Hayashi Y Stafford BJ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(8):839-843
The extensor and flexor group muscles and their related muscles were functional-morphologically observed in the dead body of the giant panda to clarify the action of the forearm and the palm in the manipulation of the species. The Musculus flexor carpi ulnaris had two developed heads, however, we can conclude that the contraction of this muscle slightly changes the angle of the accessory carpal bone to the ulna. The data pointed out that the accessory carpal bone acts as a supporting post, when the giant panda seizes the object. The M. abductor digiti I longus possessed the well-developed origin in both ulna and radius. These findings suggest that this muscle may function as a supinator of the forearm. We also suggest that the well-developed M. pronator quadratus and M. pronator teres, and the proximal part of the M. abductor digiti I longus and the M. supinator may efficiently contribute to the pronator-spinator action of the forearm, when the giant panda brings the food to its mouth using the manipulation system equipped in the palm region. 相似文献
94.
Kobayashi T Yura T Yanagi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(2):177-180
Expression of 150 kda oxygen-regulated protein, ORP150, was examined in the atheromatous lesions on aortic valves in high-fat diet fed mice. Immunohistochemical staining revealed that ORP150 was expressed on the surface of plaque and was co-localized with phagocytes bearing Mac-3, a mouse macrophage differentiation antigen. These findings suggest that ORP150 is involved in the development of the atheromatous plaque. Titer of autoantibody against ORP150 was gradually elevated in parallel with the length of period of high-fat diet feeding. These results suggest that the deposition of immunocomplex toward ORP150 antigen is involved in atheromatous plaque progression. 相似文献
95.
Kazami A Watanabe H Hayashi T Kobayashi K Ogawa Y Yamamoto K Adachi Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(8):735-737
In 1996 and 1997, the seroprevalence against Leptospira in parturient sows with premature birth or stillbirth from two herds was investigated. In three out of four sow serum samples obtained in Gunma Prefecture, the antibody titers to Leptospira interrogans serovar copenhageni (M20) were higher than 10,000 (the reciprocal of the serum dilution). Furthermore, the antibody titers to L. interrogans serovar canicola (Hond Utrecht IV) were significantly high in the three sows and the titers ranged from 1,000 to 3,000. In sows obtained in Chiba Prefecture, significantly high antibody titers to serovar copenhageni (M20) were confirmed in eight out of 40 sows, and antibody titers greater than 10,000 in six of them. Significantly high antibody titers to L. interrogans serovar icterohaemorrhagiae (RGA) and L. canicola (Hond Utrecht IV) were confirmed in four and 18 out of the 40 sows, respectively, compared with the titers to the other serovars. These findings may indicate the prevalence of leptospirosis in pig herds in both Gunma and Chiba Prefectures. 相似文献
96.
97.
Sheauchi Cheng Yukio Hiwatashi Hideki Imai Mitsuru Naito Tatsuka Numata 《Journal of Forest Research》1998,3(4):199-204
The major causes of deforestation and degradation of natural resources in the Belete-Gera Forest of Ethiopia are coffee production
activities and encroachment into forestland to expand farmland and pasture. Population growth, and the government’s land-reform
and re-settlement programs have caused local residents to lose harmony with the land. Forest management in this area hasn’t
yet been fully developed. The objectives of this study are to identify the extent of deforestation and natural resource degradation,
in preparation for a sound management plan. Encroachment of farmland and pasture into natural forest during the past four
years has been identified through interviews and aerial photo interpretation. The encroachment rate is 1.45% per year. Encroachment
occurred mostly on areas with gentle slopes adjacent to populated villages and along roads and footpaths. The extent and impact
of coffee production activities were examined through agency documents, forest survey data and vegetation survey. It is estimated
that up to 49% of the accessible natural forest is under the influence of coffee production activities, among which collecting
of naturally grown coffee beans has the least and the coffee plantations has the most impact on the natural forest. Coffee
plantations in natural forest have reduced the forest density and species diversity. Age structure of the trees is limited
to mature and old classes only, which eventually endangers their function as shade for coffee plantations.
This project was funded by the Japan International Cooperation Agency. 相似文献
98.
Gaku Kanno Takahito Yamaguchi Hideki Kishimura Etsurou Yamaha Hiroki Saeki 《Fish physiology and biochemistry》2010,36(3):637-645
Trypsin from the pyloric ceca of masu salmon (Oncorhynchus masou) cultured in fresh water was purified by a series of chromatographies including Sephacryl S-200, Sephadex G-50 and diethylaminoethyl
cellulose to obtain a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) and native PAGE.
The molecular mass of the purified trypsin was estimated to be approximately 24,000 Da by SDS–PAGE. The enzyme activity was
strongly inhibited by phenylmethylsulfonyl fluoride, soybean trypsin inhibitor, and N
α
-p-tosyl-l-lysine chloromethyl ketone. Masu salmon trypsin was stabilized by calcium ion. The optimum pH of the masu salmon trypsin
was around pH 8.5, and the trypsin was unstable below pH 5.0. The optimum temperature of the masu salmon trypsin was around
60°C, and the trypsin was stable below 50°C, like temperate-zone and tropical-zone fish trypsins. The N-terminal 20 amino acid sequence of the masu salmon trypsin was IVGGYECKAYSQPHQVSLNS, and its charged amino acid content was
lower than those of trypsins from frigid-zone fish and similar to those of trypsins from temperate-zone and tropical-zone
fish. In the phylogenetic tree, the masu salmon trypsin was classified into the group of the temperate-zone fish trypsin. 相似文献
99.
Hideki ENDO Truong Son NGUYEN Dinh Duy NGUYEN Motoki SASAKI Junpei KIMURA Tatsuo OSHIDA Masaya YAGO Ngoc Tu LY Thi Tham NGUYEN Masaharu MOTOKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(10):1534
Morphological variation of the skull was examined in the northern treeshrew (Tupaia belangeri) from various localities across Southeast Asia. Through a multivariate analysis, the treeshrews from South Vietnam exhibited distinct morphological characteristics compared to other populations from Thailand and Laos, and Malaysia. The plots of the specimens of North Vietnam are not randomly mixed with Thailand plots segregation in the scatteregrams of canonical discriminant analysis. Since the skulls of the population from North Vietnam were morphologically similar to those form central Laos and northern and northeastern Thailand, the zoogeographical barrier effect of Mekong River was not clearly confirmed. The population of the Kanchanaburi in western Thailand is clearly smaller in size compared to the other populations. The southern border of the distribution of this species is determined by the Isthmus of Kra or Kangar-Pattani Line. In the northern treeshrew, which is distributed from southern China to Bangladesh and southern Thailand, we have detected osteometrical geographical variation driven by geography. These results indicate that the skull morphology in the Tupaia glis-belangeri complex distinctively differs in South Vietnam, western Thailand, and southern Thailand. The zoogeographical barrier and factor separating these districts are expected to clarify in the future. 相似文献
100.
Tadahide Kurokawa Takuma Okamoto Koichiro Gen Susumu Uji Koji Murashita Tatsuya Unuma Kazuharu Nomura Hajime Matsubara Shin‐Kwon Kim Hiromi Ohta Hideki Tanaka 《Journal of the World Aquaculture Society》2008,39(6):726-735
The occurrence of morphological deformities under different rearing water temperatures (18, 20, 22, 24, 26, 28, and 30 C) was examined in Japanese eel larvae. The rates of hatching and survival until yolk resorption at 22–26 C were higher than those at other water temperatures. Fertilized eggs never hatched at 18 and 30 C. The rates of occurrence of abnormal larvae reared at the water temperatures 24–28 C were lower than those at 20 or 22 C. Pericardial edema and lower jaw deformities occurred most frequently at lower temperatures (20 and 22 C). In contrast, the incubation temperature did not significantly affect the relative frequency of some neurocranial deformities and of spinal curvature. These results imply that the optimal temperatures for rearing Japanese eel eggs and embryos are 24–26 C from the viewpoints of survival and deformity. 相似文献