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221.
Katsuyuki HAMASAKI Hideyuki IMAI Nobuhiko AKIYAMA Kyohei FUKUNAGA 《Fisheries Science》2004,70(6):988-995
ABSTRACT: Females of the swimming crab Portunus trituberculatus were cultured under natural temperature and high temperature (21°C) conditions to examine ovarian development and oviposition from autumn (October) to the beginning of breeding season in the following spring (May). Ovaries developed because of vitellogenesis of oocytes from October to December and their developmental state did not change during the overwintering period from December to March. In spring, from late March to mid May, some ovaries reached prematuration and maturation stages and ovipositions began from mid–late April under natural temperature conditions. Females reared in tanks of high temperature regimes oviposited earlier than those reared in tanks of natural temperatures. The number of days to oviposition tended to decrease with advancement of the beginning times (early February to late April) of culture of females under high temperature regimes; induced ovipositions were achieved more easily with increasing photoperiod from c . 12 h in early February to c . 14 h in late April. Therefore, it is inferred that the temperature and photoperiod are important environmental factors controlling ovarian development and oviposition. 相似文献
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Howlett NG Taniguchi T Olson S Cox B Waisfisz Q De Die-Smulders C Persky N Grompe M Joenje H Pals G Ikeda H Fox EA D'Andrea AD 《Science (New York, N.Y.)》2002,297(5581):606-609
Fanconi anemia (FA) is a rare autosomal recessive cancer susceptibility disorder characterized by cellular hypersensitivity to mitomycin C (MMC). Six FA genes have been cloned, but the gene or genes corresponding to FA subtypes B and D1 remain unidentified. Here we show that cell lines derived from FA-B and FA-D1 patients have biallelic mutations in BRCA2 and express truncated BRCA2 proteins. Functional complementation of FA-D1 fibroblasts with wild-type BRCA2 complementary DNA restores MMC resistance. Our results link the six cloned FA genes with BRCA1 and BRCA2 in a common pathway. Germ-line mutation of genes in this pathway may result in cancer risks similar to those observed in families with BRCA1 or BRCA2 mutations. 相似文献
225.
Shotaro NISHIMURA Ayano SAGARA Ichiro OSHIMA Yoshitaka ONO Hisao IWAMOTO Kaoru OKANO Hideyuki MIYACHI Shoji TABATA 《Animal Science Journal》2009,80(4):451-459
The distribution and three-dimensional architecture of collagen fibers were compared between pig, goat and chicken livers. Immunohistochemical staining revealed that collagen type I was identified in the interlobular connective tissue region and intralobular areas in pigs and goats. Type III collagen was also identified in the interlobular connective tissue region and intralobular sinusoidal walls. In the chicken liver, only the circumference region of the vessels was immunostained with collagen type I and III antibodies and the interlobular connective tissue wall could not be distinguished clearly. In the intralobular region, collagen type I antibody immunoreacted around the hepatic cells but collagen type III antibody immunoreacted weakly. In the NaOH macerated specimen, well-developed collagen bundles formed the prominent interlobular walls in pigs. In contrast, the wall in the goat liver comprised a thin layer of the bundles. In the chicken liver, there were no notable collagen septa between lobules. The intralobular collagen construction was quite different between the animals, indicating a fragile collagen fibril networks in pigs, a robust framework in goats and dense fabric-like septa in chickens. These results indicate that the distinct collagen frameworks may contribute to the histological strength of the livers in each of the animal species. 相似文献
226.
Naoyuki Matsunaga Shin-ichi Chisada Hideyuki Fujioka Kana Takashima Nozomu Okino Makoto Ito 《Fisheries Science》2011,77(4):583-590
Vibrios are highly motile, facultatively anaerobic bacteria that are ubiquitous in aquatic environments and part of the normal
intestinal microflora of healthy fish, but some species can cause vibriosis. The adherence of vibrios to host fish intestines
is a significant event not only for their survival and growth, but also in terms of pathogenicity. However, the molecular
mechanism underlying the adhesion of vibrios to the intestinal tract of fish is not fully understood. We report here the identification
of intestinal glycosphingolipid (GSL) receptors to which pathogenic vibrios attach in typical mariculture fish. Thin-layer
chromatography overlay assays using five species of 35S-labeled vibrios and intestinal glycosphingolipids of seven species of mariculture fish revealed that all of the fish tested
possessed GM3 (NeuAcα2-3Galβ1-4Glcβ1-1′Cer) and/or GM4 (NeuAcα2-3Galβ1-1′Cer) as major acidic intestinal GSLs and that all
of the vibrios tested specifically adhered to GM3 and/or GM4. Our results demonstrate that these GSLs were able to function
as a receptor for the various vibrios tested. Analysis of the relationship between sugar structure and receptor activity for
vibrios revealed that ‘NeuAcα2-3Galβ1-’ is required at the non-reducing end of glycosphingolipids for the bacteria to attach. 相似文献
227.
- 1. The vertical and horizontal distribution of unionoid mussels (Pronodularia japanensis) were examined in spring, late summer and winter for different size classes, including juveniles (<20 mm) and large adults (>50 mm), in an agricultural drainage channel in Central Japan.
- 2. A large number of small‐sized juveniles were found at the surface (0–3 cm depth) in spring, whereas approximately half of the adults remained in the deep layer (3–8 cm depth). Most individuals were found in the surface layer irrespective of size classes in summer, and approximately 70% of all individuals descended to the deep layer in winter.
- 3. Horizontal distribution did not substantially change across seasons and high density patches were consistently found in the areas where substrate appeared to be stable or hydraulically sheltered. Spatial distribution of small juveniles was relatively well predicted by the abundance of adults.
- 4. The size–frequency distribution was multi‐modal in three seasons and estimated numbers of newly recruited juveniles were highly variable in recent years, suggesting that juveniles were rarely found owing to irregular reproduction and/or recruitment not the overlooked habitat preferred by small‐sized juveniles.
- 5. As P. japanensis is characterized by the behaviour of burrowing down to a depth of ~10 cm, maintaining the processes of sediment deposition appears to be one of the critical criteria for the conservation of mussel habitats in agricultural drainage channel systems, which are commonly lined with smooth concrete materials. Copyright © 2010 John Wiley & Sons, Ltd.