Production of Diabetic Offspring Using Cryopreserved Epididymal Sperm by In Vitro Fertilization and Intrafallopian Insemination Techniques in Transgenic Pigs     

Kazuhiro UMEYAMA  Kasumi HONDA  Hitomi MATSUNARI  Kazuaki NAKANO  Tatsuro HIDAKA  Keito SEKIGUCHI  Hironori MOCHIZUKI  Yasuhiro TAKEUCHI  Tsukasa FUJIWARA  Masahito WATANABE  Masaki NAGAYA  Hiroshi NAGASHIMA 《The Journal of reproduction and development》2013,59(6):599-603

Somatic cell nuclear transfer (SCNT) is a useful technique for creating pig strains that model human diseases. However, production of numerous cloned disease model pigs by SCNT for large-scale experiments is impractical due to its complexity and inefficiency. In the present study, we aimed to establish an efficient procedure for proliferating the diabetes model pig carrying the mutant human hepatocyte nuclear factor-1α gene. A founder diabetes transgenic cloned pig was generated by SCNT and treated with insulin to allow for normal growth to maturity, at which point epididymal sperm could be collected for cryopreservation. In vitro fertilization and intrafallopian insemination using the cryopreserved epididymal sperm resulted in diabetes model transgenic offspring. These results suggest that artificial reproductive technology using cryopreserved epididymal sperm could be a practical option for proliferation of genetically modified disease model pigs.  相似文献   

First report of a new postharvest disease of grape caused by Cadophora luteo-olivacea     

Ryoji Nakaune  Miho Tatsuki  Hikaru Matsumoto  Yoshinori Ikoma 《Journal of General Plant Pathology》2016,82(2):116-119

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Furnish type and mat density effects on temperature and vapor pressure of wood-based panels during hot pressing     

Muhammad Navis Rofii  Shuto Kubota  Hikaru Kobori  Yoichi Kojima  Shigehiko Suzuki 《Journal of Wood Science》2016,62(2):168-173

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Stand-scale transpiration estimates in a Moso bamboo forest: (I) Applicability of sap flux measurements     

Tomonori Kume  Yuka Onozawa  Hikaru Komatsu  Kenji Tsuruta  Yoshinori Shinohara  Toshihiro Umebayashi  Kyoichi Otsuki 《Forest Ecology and Management》2010

The applicability of sap flux (F_d) measurements to bamboo forests has not been studied. This study was undertaken to establish an optimal and effective design for stand-scale transpiration (E) estimates in a Moso bamboo forest. To this aim, we validated F_d measurements in Moso bamboos in a cut bamboo experiment. In addition, we analyzed how sample sizes affect the reliability of E estimates calculated from F_d and conducting culm area (A_{S_b}). In the cut bamboo experiments, we found that F_d measurement using a 10 mm probe was a valid means of determining the water-use behavior of a Moso bamboo, although a specific correction was needed. Furthermore, we calculated E from stand A_{S_b} (A_{S_stand}) and mean stand F_d (J_S). Employing Monte Carlo analysis, we examined potential errors associated with sample size in E, A_{S_stand}, and J_S using an original dataset with A_{S_b} and F_d measured for 40 and 16 individuals, respectively. Consequently, we determined the optimal sample size for both A_{S_stand} and J_S estimates as 11. The optimal sample sizes for J_S were almost the same under different vapor pressure deficit and soil moisture conditions. The optimal sample size for J_S at the study site was less than that of a coniferous plantation in the same region probably owing to small individual-to-individual variations in sap flux in the Moso bamboo forest. Our study concludes that sap flux measurements are an applicable technique for assessing water use in Moso bamboo forests.  相似文献   

Inheritance mode of male sterility in bunching onion (<Emphasis Type="Italic">Allium fistulosum</Emphasis> L.) accessions     

Ken-ichiro Yamashita  Hikaru Tsukazaki  Akio Kojima  Takayoshi Ohara  Tadayuki Wako 《Euphytica》2010,173(3):357-367

Cytoplasmic male sterility (CMS) is an indispensable trait for F₁ hybrid seed production in bunching onion (Allium fistulosum L.). Expansion of the cytoplasmic diversity of F₁ hybrid cultivars by introduction of various CMS resources has great potential to eliminate vulnerability to cytoplasm type-specific diseases. This study aimed to evaluate appearance frequency of male sterile plants in several bunching onion accessions and to identify CMS resources. In eight (‘Nogiwa Aigara’, ‘Bansei Hanegi’, ‘Amarume’, ‘Kimnung’, ‘Zhangqiu’, ‘INT/CHN/1990/GOTOU’, ‘Natsunegi’ and ‘Guangzhou’) of 135 accessions collected from Japan, China, Mongolia, Korea and Taiwan, male sterile plants appeared with varied frequencies from 1.7% (‘Nogiwa Aigara’ and ‘Bansei Hanegi’) to 24.5% (‘Zhangqiu’). The inheritance mode of Zhangqiu- and Guangzhou-derived male sterility was confirmed to be CMS by sib-crossings and interbreed crossings. Microscopic examination of microsporogenesis in the CMS plants revealed that microspore protoplasm rapidly degenerated without mitotic division after the release of microspores from tetrads. The CMS germplasm described here would be useful for the development of “A” lines to be used in F₁ hybrid seed production of bunching onion. Male fertility in ‘Nogiwa Aigara’, ‘Bansei Hanegi’, ‘Kimnung’, ‘INT/CHN/1990/GOTOU’ and ‘Natsunegi’ was verified to be controlled by a single fertility restoration locus.  相似文献   

QTL analysis of morphological traits and pseudostem pigmentation in bunching onion (<Emphasis Type="Italic">Allium fistulosum</Emphasis>)     

Hikaru Tsukazaki  Shigenori Yaguchi  Ken-ichiro Yamashita  Tadayuki Wako 《Euphytica》2017,213(7):152

Pseudostems of bunching onion (Allium fistulosum L.) show wide variation in morphological traits and skin color. However, despite being one of the most important agronomic traits, molecular studies of bunching onion pseudostems remain limited. In this study, six morphological traits (plant height, leaf length, pseudostem length, leaf width, pseudostem width and number of leaf sheaths) along with pseudostem pigmentation indices were evaluated in two field trials using an F_2:3 population derived from a single F₁ cross between a white single pseudostem (non-tillering) and a red tillering bunching onion. Plant height was highly correlated with both leaf length and pseudostem length, but not the number of leaf sheaths. In contrast, the number of leaf sheaths was significantly negatively correlated with both leaf width and pseudostem width. A total of 27 QTLs for the six morphological traits were detected in 16 regions of 11 linkage groups, with a major QTL for the number of leaf sheaths repeatedly detected on Chr. 8. Meanwhile, two QTLs associated with pseudostem pigmentation were repeatedly detected on linkage groups Chr. 4a and Chr. 5a-2. The latter (qPig5a-2) was considered a major QTL, and its location estimated by marker genotyping of the F₂ population around the qPig5a-2 region as being within a 7.6 cM interval.  相似文献   

IL‐12p40 gene expression in lung and hilar lymph nodes of MPS‐resistant pigs     

Akiko Sakuma  Shizuka Sugawara  Hikaru Hidaka  Mitsuru Nakajo  Yoshihito Suda  Tomoyuki Shimazu  Michael T. Rose  Megumi Urakawa  Tao Zhuang  Guoqi Zhao  Kouichi Watanabe  Tomonori Nochi  Haruki Kitazawa  Kazuo Katoh  Keiichi Suzuki  Hisashi Aso 《Animal Science Journal》2020,91(1)

Mycoplasma pneumonia of swine (MPS) is caused by Mycoplasma hyopneumoniae (M.hp) and is a common chronic respiratory disease of pigs. Recently, a genetically selected variant of the Landrace pig (Miyagino L2) has a lower incidence of pulmonary MPS lesions. We investigated the pathological and immunological characteristics of MPS resistance in these pigs (n = 24) by comparing with the normal landrace pig (control: n = 24). The pathological MPS lung lesion score in MPS‐selected landrace pigs was significantly lower than in the control. The gene expression of interleukin (IL)‐12p40, which acts as a chemoattractant and a component of the bioactive cytokines IL‐12 and IL‐23, was significantly higher at the hilar lymph nodes, lung, and spleen in MPS‐selected landrace pigs than in control landrace pigs, and these were negatively correlated with the macroscopic MPS lung lesion score. In summary, we demonstrate that resistance against MPS in Miyagino L2 pigs is associated with IL‐12p40 up‐regulation, in comparison with normal landrace pigs without the MPS vaccine. In addition, a comparative study of macroscopic MPS lung lesions and IL‐12p40 gene expression in lung and hilar lymph nodes may lead to beneficial selection traits for the genetic selection for MPS resistance in pigs.  相似文献   

Evaluating the durability performance of wood-based panels by a non-destructive bending test     

Yoichi Kojima  Akira Sakakibara  Hikaru Kobori  Shigehiko Suzuki 《Journal of Wood Science》2016,62(3):263-269

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Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development          下载免费PDF全文

Akiyoshi Egashira  Nobuhiko Yamauchi  Md. Rashedul Islam  Kazuki Yamagami  Asami Tanaka  Hikaru Suyama  El‐Sharawy Mohamed El‐Sayed  Shoji Tabata  Takashi Kuramoto 《Animal Science Journal》2016,87(8):1048-1054

This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1‐ and 2‐cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4‐cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2‐ to 4‐cell arrested embryos and reduce the developmental competence to blastocyst.  相似文献   

Effect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease          下载免费PDF全文

Misato Ogawa  Hironari Osada  Ayana Hasegawa  Hikaru Ohno  Nanako Yanuma  Kazuaki Sasaki  Minoru Shimoda  Junsuke Shirai  Hirotaka Kondo  Keitaro Ohmori 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):1019-1025

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