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排序方式: 共有119条查询结果,搜索用时 31 毫秒
31.
Julia Antongiovanni Joselevitch Thiago Henrique Moroni Vargas Lidia Hildebrand Pulz Karine Germano Cadrobbi Greice Cestari Huete Adriana Tomoko Nishiya Silvia Regina Kleeb José Guilherme Xavier Ricardo De Francisco Strefezzi 《Veterinary and comparative oncology》2023,21(3):401-405
Mast cell tumour (MCT) is one of the most frequent skin tumours in dogs. Due to their unpredictable biological behaviour, MCTs often cause several therapeutic frustrations, leading to investigation regarding prognostic markers. Lysyl oxidase (LOX) is an enzyme that promotes extracellular matrix stability and contributes to cell migration, angiogenesis and epithelial-mesenchymal transition. Its expression positively correlates with poor prognoses in several human and canine mammary cancers. The aim of this study was to characterise the immunohistochemical expression of LOX in MCT samples and compare it with histological grading and post-surgical survival. Twenty-six tumours were submitted to immunohistochemistry for LOX expression evaluation. All samples were positive for LOX, with variable percentages of cytoplasmic and nuclear positivity. Cytoplasmic positivity was significantly higher in high-grade MCTs (P = .0297). Our results indicate that high expression of cytoplasmic LOX in neoplastic mast cells is an indicator of poor prognosis for canine cutaneous MCTs. 相似文献
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34.
F Göritz M Dehnhard TB Hildebrandt SV Naidenko A Vargas F Martinez JV López-Bao F Palomares K Jewgenow 《Reproduction in domestic animals》2009,44(S2):87-91
The Iberian lynx is considered the most endangered felid species. Therefore, an ex situ conservation program was initiated to protect this species from extinction. Additional knowledge on lynx reproduction biology and reliable methods for reproductive monitoring are important for developing a captive breeding program. The aim of this study in lynx was to implement transrectal ultrasonography to visualize ovarian structures (follicles, corpora lutea) and to assess ovarian activity in addition to analysis of serum progesterone and oestradiol. Because of limited access to Iberian lynxes, the less-endangered Eurasian lynx and bobcat were also studied in this comparative study. Recent endocrinological studies based on faecal and urinary progesterone and oestrogen metabolites revealed that steroid profiles in both these species were alike and did not follow the typical pattern of other felids. Pregnancy diagnosis was not possible, since progesterone concentrations did not differ between pregnant and pseudopregnant animals. Progesterone was also detected after parturition as well as after weaning until the onset of a new oestrous cycle. In the present study, the presence of corpora lutea during the non-breeding season was confirmed by ultrasonography and by elevated serum levels of progesterone averaging 3.56 ± 1.3 ng/ml in Eurasian and 6.1 ± 0.26 ng/ml in Iberian lynx, respectively. The ultrasonographical findings on the ovarian structures suggest strongly that corpora lutea developed after ovulation stay active until November and regress before the onset of the next oestrus. 相似文献
35.
Investigations using sustained-release deslorelin implants at various insertion sites have shown that this method consistently induces oestrus in anoestus bitches. However, fertility comparisons between implant insertion sites have not been performed. Anestrous beagle bitches received one 2.1 mg deslorelin implant beneath the vestibular mucosa (VM group; n = 6) or in the subcutaneous tissue between the shoulder blades (SubQ group; n = 8). Vestibular implants were removed when serum progesterone concentrations first exceeded 1.5 ng/ml. Vaginal cytologies and blood samples were collected daily and bitches were inseminated during oestrus. Serum progesterone and deslorelin concentrations were measured and pregnancy status was determined using ultrasonography. There were no differences between groups in the intervals between implant administration and the onset of proestrus, the time of the luteinizing hormone surge and the onset of cytologic diestrus. There were also no differences in the number of corpora lutea or foetuses. However, conception rate was significantly lower in the SubQ group. The pregnancy rate did not differ significantly between the VM and SubQ groups [4 out of 6 (66.7%) and 3 out of 8 (37.5%), respectively]. One bitch (16.7%) in the VM group and three bitches (37.5%) in the SubQ group suffered distinct, premature declines in serum progesterone concentrations starting 1–4 weeks after cytologic diestrus. Serum progesterone concentrations did not recover (premature luteal failure), resulting in abortion. Bitches with premature luteal failure in the SubQ group still had serum deslorelin concentrations >100 pg/ml 20 days after implant insertion, suggesting a possible association between prolonged deslorelin release and luteal failure. 相似文献
36.
Mario S. Blattner Sabine Augustin Helmer Schack‐Kirchner Ernst E. Hildebrand 《植物养料与土壤学杂志》2000,163(6):583-587
Although the composition of the soil solution has important ecological information, there is no general consensus for obtaining and analyzing of the soil solution. This study presents an alternative procedure to obtain the soil solution and determine all relevant anions and cations. The soil samples are taken with an auger. 10—20 g of field moist soil are desorbed in a pressure chamber at 170 kPa (pF 3.2), with a cellulose acetate membrane filter (∅︁ < 0.45 μm) as capillar bridge between the interior and exterior of the chamber. The desorption procedure is performed at 4°C for 24 hours and yields up to 1.0 ml soil solution, depending on the actual water potential. If more soil solution is needed, the soil may be replaced by another aliquot of the same sample. 0.15 ml of soil solution is sufficient for analysing all cations and anions, which account quantitatively for the ion balance with a capillary electrophoresis. Compared with suction cups, ion concentrations in desorption solutions are, although generally lower, in the same order of magnitude. The advantage of this method is that no field equipment is needed, apart from the auger. Even in heterogeneous forest soils, water soluble ions can be monitored with a high spatial resolution and without any dilution effects, which are common in the most laboratory methods. The problem of lacking spatial representativity in stationary lysimeter stations is also overcome. Additionally it is possible to obtain and analyze soil solutions in a suction range where suction cups fail. 相似文献
37.
J Christensen-Dalsgaard W Dappen SV Ajukov ER Anderson HM Antia S Basu VA Baturin G Berthomieu B Chaboyer SM Chitre AN Cox P Demarque J Donatowicz WA Dziembowski M Gabriel DO Gough DB Guenther JA Guzik JW Harvey F Hill G Houdek CA Iglesias AG Kosovichev JW Leibacher P Morel CR Proffitt J Provost J Reiter EJ Rhodes FJ Rogers IW Roxburgh MJ Thompson RK Ulrich 《Science (New York, N.Y.)》1996,272(5266):1286-1292
Data from the Global Oscillation Network Group (GONG) project and other helioseismic experiments provide a test for models of stellar interiors and for the thermodynamic and radiative properties, on which the models depend, of matter under the extreme conditions found in the sun. Current models are in agreement with the helioseismic inferences, which suggests, for example, that the disagreement between the predicted and observed fluxes of neutrinos from the sun is not caused by errors in the models. However, the GONG data reveal subtle errors in the models, such as an excess in sound speed just beneath the convection zone. These discrepancies indicate effects that have so far not been correctly accounted for; for example, it is plausible that the sound-speed differences reflect weak mixing in stellar interiors, of potential importance to the overall evolution of stars and ultimately to estimates of the age of the galaxy based on stellar evolution calculations. 相似文献
38.
Hildebrand EM 《Science (New York, N.Y.)》1945,102(2639):101-102
39.
Hildebrand JH 《Science (New York, N.Y.)》1955,121(3155):840-843
40.
SV AA Henriksen R.J. Jørgensen P. Nansen KR Sejrsen J. Brolund Larsen S. Klausen 《Veterinary parasitology》1976,2(3):259-272
The investigation was designed to study the gastro-intestinal helminth infection established in yearling calves with reference to fluctuations in the larval contamination of the herbage during the grazing season. In a 2 × 3 factor experiment, comprising six groups, each of six calves, a comparison was made between infection levels, growth rates etc. in calves grazing the same paddock over an entire season and calves moved before the July rise in herbage contamination to paddocks not grazed earlier in the same season. A comparison was also made between animals receiving no anthelminthic treatment, animals treated 3 weeks after the start of the grazing season and again when moved, and animals treated every 3 weeks during the season.Before July there were no weight gain differences between the various groups of calves. During the rest of the season, the calves which were moved gained 647–869 g, while those that remained on the same paddock, and were exposed to a high level of larval contamination gained only 81–361 g per day. The effect of anthelmintic treatment was less pronounced, though significant. The weight gains were correlated to a high degree with the larval contamination levels of the respective paddocks (r = ? 0.79) as well as with the serum pepsinogen (r = ? 0.75) and albumin levels (r = 0.75) of the calves and to a minor degree also with faecal egg counts (r = ? 0.45). The observed weight gain differences could not be accounted for by differences in grass quantity or quality. 相似文献