Development and validation of a soil-based geographic information system model of habitat of Fossaria bulimoides, a snail intermediate host of Fasciola hepatica     

Stanley H. Zukowski  John M. Hill  Farrell W. Jones  John B. Malone 《Preventive veterinary medicine》1991,11(3-4):221-227

Soil maps were investigated for their potential to indicate the spatial distribution of habitat of the amphibious snail, Fossaria bulimoides, the intermediate host of Fasciola hepatica in Louisiana. On a 760 ha farm adjacent to the Gulf of Mexico, most snail habitats clustered along interfaces of cheniers (relict beaches with sandy soils) with marsh (heavy, gleyed clays). A few small foci occurred on the cheniers above interfaces. Virtually no habitats occupied marsh away from these interfaces, on this or several other farms previously examined. Habitat and soil map overlays were analyzed in a raster-based geographic information system (GIS) with a 10 m pixel size; a model of habitat was generated as bands 50–90 m wide at chenier-marsh interfaces. The model was extrapolated to a 29 000 ha study area and tested on 12 additional farms not adjacent to the Gulf. On these farms, snail surveys were carried out as transects on three strata: waveface (WF, former beachfront) and backslope (BS) chenier-marsh interfaces, and chenier. The perimeters of all habitats encountered were outlined. The interface of marsh with chenier backslopes occupied 5.25% of the area of the farms, but held 21.69% of snail habitat area. Additionally, chenier soils of the Hackberry-Mermentau complex (HM) occupied 12.32% of farm area, but held 61.39% of habitat. Habitat area was related by regression analysis to the proportion of farm consisting of HM soils (r² = 0.41, P < 0.05).  相似文献   

The resistance of meat chickens vaccinated by aerosol with a live V4 Newcastle disease virus vaccine in the field to challenge with a velogenic Newcastle disease virus     

IG BELL  PJ NICHOLLS  C. NORMAN  AINI IDERIS†  GM CROSS† 《Australian veterinary journal》1991,68(3):97-101

Meat chickens housed on a commercial broiler farm in Australia were vaccinated once at 10 to 11 days-of-age by aerosol with live V4 Newcastle disease virus (NDV) vaccine. Groups of vaccinated and unvaccinated birds were flown to Malaysia, where they were challenged with a virulent strain of NDV. Survival rates in vaccinated chickens challenged 7, 14, 21 or 31 d after vaccination were 0.47, 0.77, 0.97 and 0.92, respectively. All unvaccinated chickens died due to Newcastle disease (ND) following challenge. Chickens in Australia and Malaysia were bled and the serums tested for haemagglutination-inhibiting (HI) antibody to NDV. Many vaccinated birds with no detectable antibody, and all birds with a log2 titre of 2 or greater, survived challenge. The results showed that this V4 vaccine induced protective immunity in a significant proportion of chickens within 7 d of mass aerosol vaccination. This early immunity occurred in the absence of detectable circulating HI antibody. Non-HI antibody mediated immunity continued to provide protection up to 31 d after vaccination. Almost all vaccinated birds were protected within 3 w of vaccination. It is concluded that the V4 vaccine is efficacious and could be useful during an outbreak of virulent ND in Australia.  相似文献   

The retrospective diagnosis of a second outbreak of equine morbillivirus infection   总被引：5，自引：0，他引：5  

PT HOOPER  AR GOULD  GM RUSSELL  JA KATTENBELT  G. MITCHELL 《Australian veterinary journal》1996,74(3):244-245

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Vaccination of cows with rough Escherichia coli mutants fails to protect against experimental intramammary bacterial challenge     

A. W. Hill 《Veterinary research communications》1991,15(1):7-16

Vaccination of cows with rough Escherichia coli mutants fails to protect against experimental intramammary bacterial challenge. Vaccine A, a heat-killed Re mutant of strain K12, (UB 1574), was administered as a single parenteral and local dose to 5 cows with 3 control animals and Vaccine B, a heat-killed mutant of O111:B4 (J5) was administered as three parenteral doses into 5 cows with 5 control animals. Following intramammary challenge with a smooth wild-type strain (P4), an acute, severe clinical mastitis developed in all 14 quarters (9 vaccine A and 5 vaccine B) of the vaccinated animals which was indistinguishable from that in the 11 quarters of the control animals. Following vaccine B there was an elevation in serum IgG1 and IgG2 antibody to the common core antigen of endotoxin which, in contrast to the control animals, showed a further increase after intramammary infection.  相似文献   

Tomato Production and Soil Salt Distribution under Line-Source Trickle Irrigation1     

A. M. Abu-Awwad  R. W. Hill 《Journal of Agronomy and Crop Science》1991,167(3):188-195

Yield of red cherry tomatoes and soil salt distribution as affected by different amounts and salinities of irrigation water was studied in a field plot experiment during the summer of 1988 at Utah State University. Four different amounts of fresh water and two amounts of saline water were imposed with line source trickle irrigation on a sandy loam soil in lysimeters. Tomato yield and seasonal evapotranspiration increased in a linear fashion with increasing irrigation water. Tomato yields with fresh water (EC = 0.33 dS/m) were significantly higher than with saline (EC = 4.0 dS/m) water. There were no significant differences in seasonal evapotranspiration between the two salinity levels with two levels of deficit irrigation. Soil water depletion and availability decreased as the soil water salinity increased. Soil water salinities increased with both vertical and horizontal distance from trickle lines and reached a maximum at the bottom of the wetted area and between trickle lines.  相似文献   

Subcellular distribution of copper in the liver of fetal deer in the last month of gestation   总被引：2，自引：0，他引：2  

M J Leighton  T J Peters  R Hill  G D Smith  W Holt  D M Jones 《Research in veterinary science》1990,49(3):298-305

Fetal deer, in the last month of gestation, accumulate high concentrations of copper in the liver. Livers from fetal deer in late gestation were homogenised and fractionated by continuous sucrose density gradient centrifugation. The distribution of copper closely followed that of DNA; approximately two thirds of the metal was localised to the nuclear fractions with the remaining third in the cytosolic fractions. The fractionation procedure was repeated with digitonin, a lysosomal perturbant: lysosomes were disrupted and the marker enzyme, N-acetyl-beta-glucosaminidase, shifted from the particulate fractions to the cytosolic fractions; the distribution of copper was unaffected. Differential centrifugation of homogenate confirmed that approximately two thirds of the copper was associated with the nuclear fraction. Further confirmation of a nuclear localisation of copper was provided by X-ray microanalysis of purified nuclei.  相似文献   

Histologic and histochemical changes in articular cartilages of immature beagle dogs dosed with difloxacin, a fluoroquinolone   总被引：6，自引：0，他引：6  

J E Burkhardt  M A Hill  W W Carlton  J W Kesterson 《Veterinary pathology》1990,27(3):162-170

The histologic and histochemical features of quinolone-induced arthropathy were studied using 14 skeletally immature Beagle dogs (3 to 4 months old) dosed orally with difloxacin at 300 mg/kg body weight once daily for 1, 2, 5, or 7 days. A placebo was given to eight other age-matched Beagle dogs that served as controls. A scoring technique that included lesion size and histologic features was used to determine the progression of lesions. Articular-epiphyseal cartilage complexes on the femoral and humeral heads and tibial tarsal bone were identified as predilection sites. Within predilection sites on femoral and humeral heads, lesions developed in specific areas. Lesions appeared within 2 days of the onset of treatment, and lesion scores increased with time. Grossly, the lesions were raised, fluid-filled vesicles on the articular surface. Histologic changes included vesicle formation with loss of proteoglycan, clumping of unmasked collagen, and degeneration and necrosis of chondrocytes. In lesions with higher scores, chondrocytes were often in clusters or they were undergoing metaplasia toward spindle-shaped cells. Although dissolution of matrix and necrosis of chondrocytes were typical of all lesions, smaller lesions had histologically normal chondrocytes adjacent to small vesicles. In sections stained with toluidine blue, proteoglycan was aggregated with collagen fibrils or was absent from the matrix adjacent to vesicles. Unique features, such as biomechanical forces, may predispose specific areas of articular cartilage to develop lesions.  相似文献   

Morphological and molecular analysis of androgenetic, selfed and backcrossed plants produced from a Hordeum vulgare x H, bulbosum hybrid     

M. J. gilpin  R. A. pickering  A. G. fautrier  D. L. mcneil  G. szigat  A. M. Hill  R. G. Kynast 《Plant Breeding》1997,116(6):505-510

Anther culture (AC) was carried out on a fertile triploid hybrid between Hordeum vulgare L. (cultivated barley) and H. bulbosum L, (bulbous barley grass) to determine whether AC-derived regenerants differed from progeny obtained through selfing and backcrossing. Chromosome counts were carried out on all plants and DNA was extracted from them to prepare Southern blots for molecular analysis. To identify true recombinants, the blots were probed with rye repetitive sequence probes (pSc119.1 and pScl19.2). which hybridize strongly and specifically to H. bulbosum DNA. Twenty probes that detect single- or low-copy sequences were hybridized with Southern blots containing restricted DNA extracted from 25 AC-derived plants, 11 selfed and six backcrossed progeny that showed hybridizations with pScll9. Although restriction fragment length polymorphisms (RFLPs) were only observed using probes that map to four of the possible 14 chromosome arms, an introgression associated with chromosome 6HS was frequently observed among plants derived from AC. selfing and backcrossing. Plants from AC differed from selfed and backcrossed progeny in their chromosome number; unique RFLP bands that were occasionally observed may indicate chromosomal rearrangements.  相似文献   

Effects of Interspecific Competition on the Inheritance of Quantitative Traits in White Clover *)     

J. Hill 《Plant Breeding》1993,110(3):212-219

Analysis of a half diallel cross among four white clover cultivars, grown as monocultures and in mixtures (duocultures) with two separate perennial rye-grass cultivars, confirmed that for stolon and leaf number a higher proportion of the genetic variance in duocultures was non-additive. For canopy height and dry weight, however, this proportion was less affected by inter-specific competition. An additive-dominance model of gene action sufficed for all four characters in monocultures, but in duocultures epis-tasis was just one of the factors complicating the genetic picture. This, coupled with short-term reversals in the direction of dominance, merely illustrates the complexity of breeding white clover cultivars. Possible future developments in the breeding of white clover are discussed in the light of these results.  相似文献   

Improved plant heterokaryon formation by surfactant-supplementation of polyethylene glycol fusogen solution     

T. K. Hill  M. R. Davey  J. B. Power  P. Anthony  K. C. Lowe 《Euphytica》1995,85(1-3):269-273

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