全文获取类型
收费全文 | 1056篇 |
免费 | 35篇 |
国内免费 | 1篇 |
专业分类
林业 | 120篇 |
农学 | 44篇 |
137篇 | |
综合类 | 31篇 |
农作物 | 38篇 |
水产渔业 | 98篇 |
畜牧兽医 | 530篇 |
园艺 | 6篇 |
植物保护 | 88篇 |
出版年
2023年 | 1篇 |
2022年 | 12篇 |
2021年 | 44篇 |
2020年 | 23篇 |
2019年 | 12篇 |
2018年 | 15篇 |
2017年 | 22篇 |
2016年 | 34篇 |
2015年 | 59篇 |
2014年 | 68篇 |
2013年 | 85篇 |
2012年 | 67篇 |
2011年 | 71篇 |
2010年 | 42篇 |
2009年 | 55篇 |
2008年 | 68篇 |
2007年 | 60篇 |
2006年 | 75篇 |
2005年 | 58篇 |
2004年 | 62篇 |
2003年 | 62篇 |
2002年 | 44篇 |
2001年 | 12篇 |
2000年 | 15篇 |
1999年 | 5篇 |
1998年 | 9篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1993年 | 1篇 |
1991年 | 2篇 |
1988年 | 2篇 |
1983年 | 1篇 |
1958年 | 1篇 |
1956年 | 1篇 |
1953年 | 1篇 |
排序方式: 共有1092条查询结果,搜索用时 15 毫秒
121.
Michimasa Oguri Kanji Okano Hajime Ieki Masayuki Kitagawa Osamu Tadokoro Yoshinori Sano Kazato Oishi Hiroyuki Hirooka Hajime Kumagai 《Animal Science Journal》2013,84(9):650-655
Three types of bamboo pellets as a ruminant feed: P1 (ground bamboo (GB) cultured with the fungus Ceriporiopsis subvermispora (CGB) : soybean curd residue (T) : soy sauce cake (S) in a 5:4:1 ratio on a dry matter (DM) basis); P2 (GB : T : S = 5:4:1 on a DM basis); and P3 (CGB : T : S = 5.5:0.8:3.7 on a DM basis) were prepared. Four wethers were assigned in a 4 × 4 Latin square design experiment to evaluate the applicability of the bamboo pellets. The experimental treatments were C (control): fed alfalfa hay cubes (AC) only, and T1, T2 and T3: fed P1, P2, and P3 with AC by 1:1 on a DM basis, respectively. The digestibility of the DM, organic matter and acid detergent fiber of P1 were significantly higher than those of P2 and P3 (P < 0.05). The total digestible nutrient (TDN) contents of AC, P1, P2 and P3 were 56.5%, 60.2%, 53.2% and 47.0%, respectively. No significant differences in nitrogen retention or ruminal pH and NH3 were observed among the treatment groups. The results indicate that bamboo pellets cultured with C. subvermispora and mainly mixed with soybean curd residue improved nutritional quality of ground bamboo because of its high digestibility and TDN content. 相似文献
122.
Fumihito Takahashi Mariko Mochizuki Touko Sato Kinya Katayama Paul R. Kenyon Stephen T. Morris Peter D. Kemp Takeyuki Ozawa Fukiko Ueda Hiroyuki Tazaki 《Animal Science Journal》2013,84(6):496-501
The establishment of a classification system for domestic animals on consumed feed stuff is thought to be important from both a hygiene and market point of view. We collected plasma samples of Romney lambs (Ovis aries) which were fed one of the following: a herb‐clover mix (n = 10) which included chicory, red clover, white clover and plantain; a plant‐grass mix (n = 10) which included plantain, ryegrass and white clover; or a grass mix (n = 10) which included ryegrass and white clover. A total of 20 elements in plasma samples obtained from the lambs were analyzed using inductively coupled plasma mass spectrometry. The data were then analyzed by principal component analysis. The lambs were divided into three groups on a score plot depending on the different feed conditions. Furthermore, discriminant analyses of the elements were examined, using linear discriminant analysis with forward stepwise regression. This discriminant function correctly classified the samples from each group. The accuracy of classification of each group, as shown by 10‐fold cross‐validation, proved the effectiveness of the established discriminant function. It is concluded that using linear discriminant analysis might be a useful tool for the validation of elements from plasma in lambs grown in different conditions. 相似文献
123.
Oocyte maturation in mammals is characterized by a dramatic reorganization of the endoplasmic reticulum (ER). In mice, the ER forms accumulations in the germinal vesicle (GV) stage and distinctive cortical clusters in metaphase II (MII) of the oocyte. Multiple evidence suggests that this ER distribution is important in preparing the oocyte for Ca2+ oscillations, which trigger oocyte activation at fertilization. In this study, we investigated the time course and illustrated the possible functional role of ER distribution during maturation of porcine oocytes by immunostaining with protein disulfide isomerase (PDI). PDI forms clusters in the cytoplasm of oocytes. After immunostaining, PDI clusters were identified throughout the cytoplasm from the GV to metaphase I (MI) stage; however, at the MII stage, the PDI formed large clusters (1–2 µm) in the animal pole around the first polar body. PDI distribution was prevented by bacitracin, a PDI inhibitor. Our experiments indicated that, during porcine oocyte maturation, PDI undergoes a dramatic reorganization. This characteristic distribution is different from that in the mouse oocyte. Moreover, our study suggested that formation of PDI clusters in the animal pole is a specific characteristic of matured porcine oocytes. 相似文献
124.
Kosei Ando Mayu Mizutani Yusuke Taniguchi Hiroyuki Yamamoto 《Journal of Wood Science》2013,59(4):290-298
To understand the viscoelasticity of wood three dimensionally, matched samples of Japanese cypress were loaded in uniaxial tensile creep in the longitudinal (L), radial (R), and tangential (T) directions at approximately 9.7 % equilibrium moisture content. Longitudinal and transverse strains were measured for the determination of viscoelastic Poisson’s ratios and three-dimensional viscoelastic compliance tensors concerning the normal strain. The changes in the transverse strains showed the same tendencies as those in the longitudinal strains, in all directions of loading. That is, during creep, the absolute value of transverse strain continued to increase with the gradual reduction in the increase rate; immediately after the removal of the load, it recovered rapidly, after which it continued to recover slowly. The transverse strain increased most easily in the T direction, followed by R and L, during creep. All the viscoelastic Poisson’s ratios and the absolute values of all elements of the viscoelastic compliance increased logarithmically with creep time. The three-dimensional viscoelastic compliance matrix for Japanese cypress is concluded to be asymmetric. 相似文献
125.
Confirmation of Novel Quantitative Trait Loci for Seed Dormancy at Different Ripening Stages in Rice
Seed dormancy contributes resistance to pre-harvest sprouting.Effects on respective quantitative trait loci (QTLs) for dormancy should be assessed by using fresh seeds before germinability altered through storage.We investigated QTLs related to seed dormancy using backcross inbred lines derived from a cross between Nipponbare and Kasalath.Four putative QTLs for seed dormancy were detected immediately after harvest using composite interval mapping.These putative QTLs were mapped near C1488 on chromosome 3 (qSD-3.1),R2171 on chromosome 6 (qSD-6.1),R1245 on chromosome 7 (qSD-7.1) and C488 on chromosome 10 (qSD-10.1).Kasalath alleles promoted dormancy for qSD-3.1,qSD-6.1 and qSD-7.1,and the respective proportions of phenotypic variation explained by each QTL were 12.9%,9.3% and 8.1%.We evaluated the seed dormancy harvested at different ripening stages during seed development using chromosome segment substitution lines (CSSLs) to confirm gene effects.The germination rates of CSSL27 and CSSL28 substituted with the region including qSD-6.1 were significantly lower than those of Nipponbare and other CSSLs at the late ripening stage.Therefore,qSD-6.1 is considered the most effective novel QTL for pre-harvest sprouting resistance among the QTLs detected in this study. 相似文献
126.
Kou HIRAGA Yumi HOSHINO Kentaro TANEMURA Eimei SATO 《The Journal of reproduction and development》2013,59(4):405-408
Localization patterns of lipid droplets in the cytoplasm of porcine oocytes were
evaluated as a novel marker for in vitro maturation (IVM) of oocytes with
high developmental competence. Porcine oocytes were cultured in TCM-199, which is a
complete synthetic medium, for 44 h at 38.5 C. Localization patterns were divided into 2
classes: lipid droplets localized uniformly in the whole cytoplasm (class I) and those
that were centrally located (class II). After IVM in TCM-199, 60% of matured oocytes
exhibited the class II pattern. To investigate the relation between the distribution of
lipid droplets and the developmental rate of the oocyte, the developmental rates of class
I and class II oocytes were compared after in vitro fertilization (IVF).
Class II oocytes showed a significantly higher rate of blastocyst development than class I
oocytes. These results suggest that porcine oocytes with high developmental competence can
be selected based on the localization patterns of lipid droplets. 相似文献
127.
128.
Fuminori TANIHARA Michiko NAKAI Hiroyuki KANEKO Junko NOGUCHI Takeshige OTOI Kazuhiro KIKUCHI 《The Journal of reproduction and development》2013,59(4):385-392
In porcine oocytes, the function of the zona pellucida (ZP) with regard to sperm
penetration or prevention of polyspermy is not well understood. In the present study, we
investigated the effects of the ZP on sperm penetration during in vitro
fertilization (IVF). We collected in vitro-matured oocytes with a first
polar body (ZP+ oocytes). Some of them were freed from the ZP (ZP− oocytes) by two
treatments (pronase and mechanical pipetting), and the effects of these treatments on
sperm penetration parameters (sperm penetration rate and numbers of penetrated sperm per
oocyte) were evaluated. There was no evident difference in the parameters between the two
groups. Secondly, we compared the sperm penetration parameters of ZP+ and ZP− oocytes
using frozen-thawed epididymal spermatozoa from four boars. Sperm penetration into ZP+
oocytes was found to be accelerated relative to ZP− oocytes. Thirdly, we evaluated the
sperm penetration of ZP+ and ZP− oocytes at 1−10 h after IVF (3 h gamete co-incubation).
The proportions of oocytes penetrated by sperm increased significantly with time in both
groups; however, the number of penetrated sperm per oocyte did not increase in ZP−
oocytes. Finally, we performed IVF using ZP− oocytes divided into control (3 h) and
prolonged gamete co-incubation (5 h) groups. Greater numbers of sperm penetrated in the 5
h group than in the control group. These results suggest that the ZP and oolemma are not
competent factors for prevention of polyspermy in our present porcine IVF system. However,
it appears that ZP removal is one of the possibilities for reducing polyspermic
penetration in vitro in pigs. 相似文献
129.
130.
Matsuura R Moriyama H Takeda N Yamamoto K Morita Y Shimamura T Ukeda H 《Journal of agricultural and food chemistry》2008,56(2):544-549
Sakura-cha (salted cherry blossom tea) is a Japanese tea that is traditionally served at celebrations such as wedding ceremonies. The production of Sakura-cha includes the immersion of cherry blossom flowers in Japanese plum vinegar, and through this process, the byproduct (plum vinegar extract of cherry blossom) is obtained. In this study, the antioxidant activity of the plum vinegar extract of cherry blossom was examined. The plum vinegar extract of cherry blossom had a greater superoxide anion scavenging activity compared with red wine, which is a well-known strong antioxidant. Liquid chromatography-mass spectrometry analysis showed that cyanidin-3-glucoside, cyanidin-3-rutinoside, and caffeic acid were the major components in the phenolic extract prepared from plum vinegar extract of cherry blossom, and they possessed superoxide anion scavenging activity. Caffeic acid is mainly responsible for the scavenging activity of phenolic extract; the contributions of cyanidin-3-glucoside and cyanidin-3-rutinoside were minor. 相似文献