Platelet-neutrophil aggregate formation in blood samples from dogs with systemic inflammatory disorders     

Dircks BH  Mischke R  Schuberth HJ 《American journal of veterinary research》2012,73(7):939-945

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Identification of Arcanobacterium (Trueperella) abortisuis, a novel species of veterinary importance, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)     

Hijazin M  Ulbegi-Mohyla H  Alber J  Lämmler C  Hassan AA  Timke M  Kostrzewa M  Prenger-Berninghoff E  Weiss R  Zschöck M 《Berliner und Münchener tier?rztliche Wochenschrift》2012,125(1-2):32-37

In the present study A. (T.) abortisuis isolated from pigs and bovines could be reliably identified by determination of phenotypic properties, genotypically by polymerase chain reaction with the help of A. (T.) abortisuis 16s-23S rDNA intergenic spacer region specific oligonucleotide primer and by Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS). The latter appeared to be a promising tool for fast and cost effective identification of this species and might help to elucidate the role A. (T.) abortisuis plays in infections of pigs, bovines, possibly other animals or humans.  相似文献   

Volumetric capnography curves as lung function test to confirm bronchoconstriction after carbachol challenge in sedated dogs     

Scheffzek S  Mosing M  Hirt R  Iff I  Moens Y 《Research in veterinary science》2012,93(3):1418-1425

This study investigated volumetric capnography (VC) in detecting airway responsiveness following airway challenge using carbachol in seven sedated dogs via face mask. Nebulised saline was administered, followed by increasing concentrations of nebulised carbachol until airflow limitation occurred (EP). Dead space (DS) variables and shape indices of the VC curve were calculated automatically after entering arterial carbon dioxide tension. Airway DS, airway DS to tidal volume (V(T)) ratio and the intercept of slope 2 of the VC curve decreased significantly at EP by 10%, 13% and 16%, respectively, minute ventilation, V(T) and alveolar DS increased significantly at EP by 49%, 22% and 200%, respectively. We conclude that VC and derived indices may be used to verify a reaction to airway challenge caused by carbachol in sedated dogs.  相似文献   

Rapid identification of the animal pathogens Streptococcus uberis and Arcanobacterium pyogenes by fluorescence in situ hybridization (FISH)   总被引：1，自引：0，他引：1  

Werckenthin C  Gey A  Straubinger RK  Poppert S 《Veterinary microbiology》2012,156(3-4):330-335

Fluorescence in situ hybridization (FISH) has been reported to be an easy and rapid identification method for many human pathogens, but applications for common veterinary pathogens are lacking. Gene probes for FISH of the animal pathogens Streptococcus uberis and Arcanobacterium pyogenes were designed to provide probes for a specific identification of these bacteria from cultures. Specific FISH probes for these species have so far not been published. Both probes recognized all isolates of the target species correctly. With the S. uberis probe SUB 196 no false-positive results were obtained for reference strains as well as for clinical isolates. Probe APYO 183 for A. pyogenes produced false-positive reactions with so far rarely described Arcanobacterium species from animals at standard hybridization conditions. In order to avoid any incorrect classifications of microorganisms as A. pyogenes, two non-labelled competitor probes were designed and successfully evaluated.  相似文献   

Greenhouse gas emissions from Silphium perfoliatum and silage maize cropping on Stagnosols     

Björn Kemmann  Thorsten Ruf  Reinhard Well  Christoph Emmerling  Roland Fuß 《植物养料与土壤学杂志》2023,186(1):79-94

Background

The sustainability of bioenergy is strongly affected by direct field-derived greenhouse gas (GHG) emissions and indirect emissions form land-use change. Marginal land in low mountain ranges is suitable for feedstock production due to small impact on indirect land-use change. However, these sites are vulnerable to high N₂O emissions because of their fine soil texture and hydrology.

Aims

The perennial cup plant (Silphium perfoliatum L.) might outperform silage maize (Zea mays L.) on cold, wet low mountain ranges sites regarding yield and ecosystem services. The aim of this study was to assess whether the cultivation of cup plant also provides GHG mitigation potential compared to the cultivation of maize.

Methods

A t-year field experiment was conducted in a low mountain range region in western Germany to compare area and yield-scaled GHG emissions from cup plant and maize fields. GHG emissions were quantified using the closed chamber method.

Results

Cup plant fields emitted an average of 3.6 ± 4.3 kg N₂O-N ha^–1 year^–1 (–85%) less than maize fields. This corresponded to 74.0 ± 94.1 g CO_2-eq kWh^–1 (–78%) less emissions per produced electrical power. However, cup plant had a significantly lower productivity per hectare (–34%) and per unit of applied nitrogen (–32%) than maize.

Conclusion

Cup plant as a feedstock reduces direct field-derived GHG emissions compared to maize but, due to lower yields cup plant, likely increases emissions associated with land-use changes. Therefore, the increased sustainability of bioenergy from biogas by replacing maize with cup plant is heavily dependent on the performance of maize at these sites and on the ecosystem services of cup plant in addition to GHG savings.  相似文献   

Use of isotope-labeled aflatoxins for LC-MS/MS stable isotope dilution analysis of foods   总被引：1，自引：0，他引：1  

Cervino C  Asam S  Knopp D  Rychlik M  Niessner R 《Journal of agricultural and food chemistry》2008,56(6):1873-1879

Aflatoxins are a group of very carcinogenic mycotoxins that can be found on a wide range of food commodities including nuts, cereals, and spices. In this study, the first LC-MS/MS stable isotope dilution assay (SIDA) for the determination of aflatoxins in foods was developed. The development of this method was enabled by easily accessible isotope-labeled (deuterated) aflatoxins B2 and G2, which were synthesized by catalytic deuteration of aflatoxin B1 and G1, purified, and well-characterized by NMR and MS. All four aflatoxins of interest (B1, B2, G1, and G2) were quantified in food samples by using these two labeled internal standards. The response factors (RF) of the linear calibrations were revealed to be matrix independent for labeled aflatoxin B2/aflatoxin B2 and labeled aflatoxin G2/aflatoxin G2. For labeled aflatoxin B 2/aflatoxin B 1 and labeled aflatoxin B2/aflatoxin G1 matrix-matched calibration was performed for the model matrices almonds and wheat flour, showing significant differences of the RFs. Limits of detection (LOD) were determined by applying a statistical approach in the presence of the two model matrices, yielding 0.31 microg/kg (aflatoxin B1), 0.09 microg/kg (aflatoxin B2), 0.38 microg/kg (aflatoxin G1), and 0.32 microg/kg (aflatoxin G2) for almonds (similar LODs were obtained for wheat flour). Recovery rates were between 90 and 105% for all analytes. Coefficients of variation (CV) of 12% (aflatoxin B1), 3.6% (aflatoxin B2), 14% (aflatoxin G1), and 4.8% (aflatoxin G2) were obtained from interassay studies. For further validation, a NIST standard reference food sample was analyzed for aflatoxins B1 and B2. The method was successfully applied to determine trace levels of aflatoxins in diverse food matrices such as peanuts, nuts, grains, and spices. Aflatoxin contents in these samples ranged from about 0.5 to 6 microg/kg.  相似文献   

Permanence of soil surface crusts on abandoned farmland in the Central Ebro Basin/Spain     

Johannes B. Ries  Ulrike Hirt 《CATENA》2008

Surface crusts are frequently found on fallow land in the semi-arid Ebro Basin (Spain) and are an important factor in land degradation. Soil surface sealing leads to a decrease in infiltration rates and a consequent increase in runoff, thereby accelerating sheet wash and rill erosion. Thin sections were used to analyse the development and structures of the different crusts found across the ridge/furrow field pattern. Rainfall simulations experiments and infiltration measurements show the runoff generation and the soil erosion rates on the crusts. The spatial distribution of crusts was documented using large-scale aerial photographs, taken from a remote-controlled hot air blimp.  相似文献   

Photolysis of imidacloprid (NTN 33893) on the leaf surface of tomato plants     

Konrad Scholz  Fritz Reinhard 《Pest management science》1999,55(6):652-654

The photolytic behaviour of the insecticide imidacloprid on the surface of tomato leaves as a result of exposure to natural sunlight was investigated. Photodegradation in sunlight was rapid and the degradation products (⩾10%) were similar to those found in plant degradation studies.  相似文献   

Gemeinsamkeiten im standortgerechten,effizienten und nachhaltigen Maisanbau für Energie- und Futterzwecke sowie zur Produktion von Körnermais     

Reinhard Amler 《Gesunde Pflanzen》2015,67(3):99-107

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Cytologic examination of fine‐needle aspirates from mammary gland tumors in the dog: diagnostic accuracy with comparison to histopathology and association with postoperative outcome     

Daniela Simon  Dorina Schoenrock  Ingo Nolte  Wolfgang Baumgrtner  Ronnie Barron  Reinhard Mischke 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(4):521-528

Background: Mammary tumors are the most common neoplasms in female dogs. Malignant tumors may carry a poor prognosis and necessitate surgery. Few data are available on the value of cytologic examination as a diagnostic or prognostic tool for mammary tumors in dogs. Objectives: The objectives of this study were to determine whether cytologic findings in fine‐needle aspirate specimens of canine mammary tumors correlate with histopathologic results and whether the cytologic diagnosis is associated with postoperative outcome. Methods: In this prospective study, fine‐needle aspirate samples were obtained from 50 mammary tumors in 50 dogs. Results of cytologic and histopathologic examination were compared, using the histologic diagnosis as the reference method. Kaplan–Meier log rank analysis was used to evaluate univariate association of the cytologic diagnosis with duration of survival, local control, and metastasis‐free interval. Results: Adequate cytologic samples were obtained in 43/50 (86%) cases. The cytologic diagnosis correlated with the histologic diagnosis for benign and malignant tumors in 40/43 (93%) and 35/43 (81%) cases, respectively. Cytologic examination had a sensitivity of 88% and a specificity of 96% for the diagnosis of malignancy. The cytologic diagnosis had significant univariate association with duration of survival (P=.016), recurrence‐free interval (P=.003), and metastasis‐free interval (P=.014). Conclusions: Cytologic examination of mammary tumors in the dog has satisfactory accuracy, sensitivity, and specificity for the diagnosis of malignancy and is associated with postoperative outcome. Further studies on the diagnostic accuracy of cytology as well as multivariate analysis of its preoperative prognostic value in mammary tumors in the dog are warranted.  相似文献