Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene     

Masashi WATANABE  Kazuaki TANAKA  Tatsuya TAKIZAWA  Kazuhito SEGAWA  Sakurako NEO  Ryo TSUCHIYA  Michiko MURATA  Masaru MURAKAMI  Masaharu HISASUE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(1):119-122

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Valproic Acid,a Histone Deacetylase Inhibitor,Decreases Proliferation of and Induces Specific Neurogenic Differentiation of Canine Adipose Tissue-Derived Stem Cells     

Yasuhiro KURIHARA  Takehito SUZUKI  Motoharu SAKAUE  Ohoshi MURAYAMA  Yoko MIYAZAKI  Atsushi ONUKI  Takuma AOKI  Miyoko SAITO  Yoko FUJII  Masaharu HISASUE  Kazuaki TANAKA  Tatsuya TAKIZAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(1):15-23

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Systemic Streptococcus dysgalactiae Subspecies equisimilis Infection in a Yorkshire Pig with Severe Disseminated Suppurative Meningoencephalomyelitis     

Kazufumi KASUYA  Eiji YOSHIDA  Rieko HARADA  Mio HASEGAWA  Hiroyuki OSAKA  Masaharu KATO  Tomoyuki SHIBAHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(5):715-718

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Expression patterns of acidic and basic fibroblast growth factor in loach fish embryos     

Kozo Inaba  Masaharu Seno  Hisaya Morino 《Fish physiology and biochemistry》1996,15(4):333-338

Heparin-binding fractions were taken from the heparin sepharose columns on which extracts of loach fish (Misgurnus anguillicaudatus) embryos from blastula, gastrula, 4–8 and 12–16 somites stages were applied. These heparin-binding fractions, except the fraction derived from 12–16 somite embryos, showed potent mitogenic activity on fibroblast-like cells derived from caudal fin blastema of goldfish. Western blot analysis of these heparin-binding fractions was carried out using monoclonal antibodies against human acidic and basic fibroblast growth factors (FGF-1 and-2). An immunoreactive FGF-1 band at 16 kD was detected in the heparin-binding fraction derived from embryos in each stage of blastula, gastrula and 4–8 somites. An immunoreactive FGF-2 band at 17 kDa was detected exclusively in the heparin-binding fraction derived from 4–8 somite embryos. In the heparin-binding fraction derived from 12–16 somite embryos neither immunoreactive FGF-1 nor-2 member band was detectable.  相似文献   

Genetic variation and phylogenetic relationships in East and South Asian melons, Cucumis melo L., based on the analysis of five isozymes     

Yukari Akashi  Naomi Fukuda  Tadayuki Wako  Masaharu Masuda  Kenji Kato 《Euphytica》2002,125(3):385-396

Genetic structure and phylogenetic relationships in East and South Asian melons were analyzed, based on the geographical variation of five isozymes. The analysis of Indian melon accessions showed a continuous variation in seed length, ranging from 4 to 13 mm. Most of the East Asian melons, vars. makuwa and conomon, were classified as the small seed type with seed length shorter than 9 mm. The frequency of the small seed type increased from the west to the east in India. Allelic variation was detected at a total of nine loci of five isozymes among 114 melon accessions. Gene diversity calculated for the nine loci indicated that Indian melon was rich in genetic variation, which decreased from India towards the east. Clear geographical variation was detected in two enzymes, APS and6-PGDH. Pgd-1 ¹ and Ap-3 ¹ were frequent in India and Myanmar, while most of the melons in Laos, China, Korea and Japan carried Pgd-1 ³ and Ap-3 ³, except var. inodorusin China. Among the latter two alleles, the frequency of Ap-3 ³ was more than 50% in the small seed type in north and east India, indicating that vars. makuwa and conomon were related to the small seed type in these areas. It was also suggested that the small seed type with wet tolerance originated in central India and was selected under wet condition in the east. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Effects of day-length and temperature on floral structure and fertility restoration in a season-dependent male-sterile Solanum villosum Mill. mutant     

Christopher O. Ojiewo  Kenji Murakami  Peter W. Masinde  Stephen G. Agong  Masaharu Masuda 《Euphytica》2007,158(1-2):231-240

Solanum villosum is an important African leafy vegetable whose yield is limited mainly by competition from early and excess fruit-set. Induced male-sterility is a potential tool to reduce this competition and enhance yields. This study was conducted to investigate the influence of photoperiod and temperature on the floral dynamics of a season-dependent male-sterile mutant. The mutant, named T-5, has flowers which are sepaloid, mostly stamenless, indeterminate and partially restored in winter, late-spring, summer and autumn, respectively. Floral organ restoration was found to be largely independent of photoperiod conditions. Day/night temperatures of 25/25 and 30/20°C were found to favour restoration of the floral organ but most flowers were stamenless and infertile. High night temperature favoured the formation of indeterminate flowers both in the growth chamber (30°C) and in the greenhouse (>25°C). On the other hand, low growth chamber (10°C) and greenhouse (<15°C) night temperature favoured the formation of sepaloid flowers. The optimum temperatures for floral structure and fertility restoration were between 20°C and 25°C (day) and 15–20°C (night). Propagation of T-5 mutant can thus be achieved by growing in regions or seasons with such temperature ranges. Under temperatures unfavourable for fruit-set, leaf productivity is expected to be high.  相似文献   

Clonality analysis of various hematopoietic disorders in cats naturally infected with feline leukemia virus     

Hisasue M  Nishigaki K  Katae H  Yuri K  Mizuno T  Fujino Y  Setoguchi A  Hasegawa A  Watari T  Masuda K  Ohno K  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(10):1059-1065

The clonality analysis of the bone marrow cells was carried out by detecting the integrated proviruses of feline leukemia virus (FeLV) to understand the pathogenesis of FeLV-associated hematopoietic disorders in cats. Bone marrow cells from 4 cases with acute myeloid leukemia (AML), 9 cases with myelodysplastic syndromes (MDS), 2 cases with pure red cell aplasia (PRCA) and 3 healthy carriers infected with FeLV were subjected to Southern blot analyses using an exogenous FeLV probe. Clonal hematopoiesis was found in all the cases with AML and in 6 of the 9 cases with MDS, but not in the cases with both PRCA and healthy carriers infected with FeLV. In the 2 cases with MDS, it was thought that the same clones of the hematopoietic cells might proliferate before and after the progression of the disease irrespective of the changes of the hematological diagnoses by cytological examination. This study indicates that MDS in cats is a disease manifestation as a result of clonal proliferation of hematopoietic cells and can be recognized as a pre-leukemic state of AML.  相似文献   

Microdetermination of Surface Proteins of Wheat Starch Granules by Dye Binding     

Masaharu Seguchi  Yomiko Yoshino 《Cereal Chemistry》1999,76(3):410-412

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Study of Internal Structure of Waxy Wheat Starch Granules by KI/I2 Solution     

Masaharu Seguchi  Takeshi Yasui  Kazuko Hosomi  Tooru Imai 《Cereal Chemistry》2000,77(3):339-342

Wheat starch granules were separated from waxy wheat flour by the acetic acid (pH 3.5) fractionation technique. Waxy wheat starch samples were stained red‐brown using KI/I₂ solution. Subsequently, the concentration of the KI/I₂ solution was increased to >2.5% KI and 1.0% I₂. Then the appearance of the waxy wheat starch granules quickly became ghostlike. The internal structure of the ghost could be observed. This structure consisted of two parts with different colors: a red‐brown central part and a slightly pinkish surrounding part. Sonication of the ghost at 20 kHz for 20 sec separated the central part from the surrounding part. Each of the separated parts were subjected to gel‐filtration chromatography, and their chromatography profiles indicated only amylopectin and no amylose. The central and surrounding parts had molecular weights of 54.3 × 10⁵ and 23.8 × 10⁶ from Berry plots, respectively.  相似文献