Hepatic AA amyloidosis in a cat: cytologic and histologic identification of AA amyloid in macrophages          下载免费PDF全文

Sakurako Neo‐suzuki  Takayuki Mineshige  Junichi Kamiie  Hideki Kayanuma  Yukako Mochizuki  Masaharu Hisasue  Ryo Tsuchiya  Kinji Shirota 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2017,46(2):331-336

A 3‐year‐old, spayed female, Domestic Shorthair cat presented with anorexia, lethargy, vomiting, probable hemoabdomen, and multiple masses on the right lateral liver lobe. Clinicopathologic and imaging abnormalities included anemia, azotemia, icterus, and hepatomegaly with hypoechoic masses. On cytologic evaluation of a fine‐needle aspiration of a liver mass there was abundant extracellular pink‐ to purple‐colored material between hepatocytes. The amorphous material was stained with direct fast scarlet (DFS), and green birefringent areas were observed under polarized light, confirming the presence of amyloid. A unique finding on the cytologic smear were macrophages containing amorphous and fibrillar amyloid‐like protein. Histopathologic examination using H&E and Congo red staining confirmed amyloid deposits within the space of Disse, along the sinusoids, portal tracts, blood vessel walls, and within the cytoplasm of macrophages. Immunohistochemical staining with anti‐AA amyloid antibodies further confirmed the presence of AA amyloid. To the author's knowledge, this is the first report of the cytologic finding of AA amyloid protein within macrophages and DFS stain detection of amyloid on a cytologic smear.  相似文献   

Neutropenia associated with osteomyelitis due to Hepatozoon canis infection in a dog     

Shimokawa Miyama T  Umeki S  Baba K  Sada K  Hiraoka H  Endo Y  Inokuma H  Hisasue M  Okuda M  Mizuno T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1389-1393

A 4-year-old, intact male Shiba dog was referred to Yamaguchi University Animal Medical Center, Yamaguchi, Japan, for the following complaints: anorexia, lethargy, intermittent fever, gingival bleeding and abdominal purpura. The dog presented with persistent neutropenia. Histopathological examination of a bone marrow sample revealed round to oval structures that resembled Hepatozoon micromerozoites and formed a "wheel-spoke" pattern. Furthermore, mature neutrophils were observed around these structures. PCR and sequencing using bone marrow aspirate confirmed Hepatozoon canis (H. canis) infection. These findings suggest that the neutropenia observed in this case was associated with osteomyelitis due to H. canis infection. This is the first report of neutropenia associated with H. canis infection. H. canis infection can be included in the differential diagnosis in canine cases of neutropenia in areas where the disease is endemic.  相似文献   

At5g54160 gene encodes <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> 5-hydroxyconiferaldehyde <Emphasis Type="Italic">O</Emphasis>-methyltransferase     

Tomoyuki Nakatsubo  Yu Kitamura  Norikazu Sakakibara  Masaharu Mizutani  Takefumi Hattori  Nozomu Sakurai  Daisuke Shibata  Shiro Suzuki  Toshiaki Umezawa 《Journal of Wood Science》2008,54(4):312-317

The function of an Arabidopsis thaliana gene, At5g54160 annotated as a caffeic acid O-methyltransferase CAOMT gene was characterized. The recombinant enzyme of this gene (AtOMT1) catalyzed the O-methylation of phenylpropanoid and flavonoid substrates. The specificity constants (k _cat/K _m) for 5-hydroxyconiferaldehyde (5-HCAld) and quercetin were both 0.11 μM⁻¹·min⁻¹. On the other hand, lignins of At5g54160-knockout Arabidopsis mutants lacked syringyl units. In addition, we showed that the gene silencing also resulted in significant accumulation of caffeyl alcohol (CaAlc). These results strongly suggested that At5g54160 gene is involved in syringyl lignin synthesis for the methylation of both 5-hydroxyconiferaldehyde and 3,4-dihydroxyphenyl compound(s). Part of this work was presented at the Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry, March 24–27, 2007  相似文献   

Treatment with a High Dose Combination of PMSG/hCG Preparation of Mares Clinically Diagnosed with Ovarian Quiescence during the Breeding Season (Investigation from 1975 to 2000)     

Tsutomu TSUKADA  Kunitada SATO  Masaharu MORIYOSHI  Masanori KOYAGO  Yutaka SAWAMUKAI 《Journal of Equine Science》2008,19(2):35-38

A total of 88 thoroughbred mares were diagnosed with clinical ovarian quiescence and subjected to four treatment regimens. Using PMSG, hCG or combinations of both. A high dose combination of 5,000IU PMSG with 5,000IU hCG showed significantly higher rates of marked estrus and ovulation induction (P<0.01) as well as conception rates (P<0.05). In the present study, the administration of a high-dose combination of PMSG with hCG was shown to be an effective treatment of ovarian quiescence in light mares.  相似文献   

Effects of prostaglandin E1 on the preparation of platelet concentrates in dogs     

Segawa K  Kondo T  Kimura S  Fujimoto A  Kato T  Ishikawa T  Neo S  Hisasue M  Yamada T  Tsuchiya R 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2012,26(2):370-376

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In vitro selective suppression of feline myeloid colony formation is attributable to molecularly cloned strain of feline leukemia virus with unique long terminal repeat     

Nagashima N  Hisasue M  Nishigaki K  Miyazawa T  Kano R  Hasegawa A 《Research in veterinary science》2005,78(2):151-154

Molecularly cloned feline leukemia virus (FeLV)-clone 33 (C-33), derived from a cat with acute myelocytic leukemia (AML), was examined to assess its relation to the pathogenesis of AML and myelodysplastic syndrome (MDS). To evaluate in vitro pathogenicity of FeLV C-33, bone marrow colony-forming assay was performed on marrow cells infected with FeLV C-33 or an FeLV subgroup A strain (61E, a molecularly cloned strain with minimal pathogenicity). The myeloid colony-forming activity of feline bone marrow mononuclear cells infected with FeLV C-33 was significantly lower than that of cells infected with 61E. This suggests that FeLV C-33 has myeloid lineage-specific pathogenicity for cats, and that FeLV C-33 infection is useful as an experimental model for investigating pathogenesis of MDS and AML.  相似文献   

Molecular detection and characterization of Haemobartonella felis in domestic cats in Japan employing sequence-specific polymerase chain reaction (SS-PCR)   总被引：2，自引：0，他引：2  

Watanabe M  Hisasue M  Hashizaki K  Furuichi M  Ogata M  Hisamatsu S  Ogi E  Hasegawa M  Tsuchiya R  Yamada T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(10):1111-1114

A novel PCR assay was developed in order to examine the prevalence of Haemobartonella felis (H. felis) in Japanese domestic cats and which was able to differentiate of the Ohio strain and the California strain of H. felis. Blood samples from a total of 21 cats suspected of having haemobartonellosis were examined employing a novel PCR assay and demonstrated positive results in 18 cats which was confirmed by cytological examination of blood smears. Four out of 18 positive cats (22%) were infected with the California strain, whilst the other 12 cats (67%) were infected with the Ohio strain and two animals (11%) were infected with both strains. As most of the cats with moderate to severe anemia were infected with the Ohio strain, it is suggested that the most prevalent strain of H. felis in Japanese domestic cats might be the Ohio strain. In the present study, it was thought that molecular detection and characterization of H. felis may provide valuable information regarding the severity and prognosis of this illness.  相似文献   

Effect of Gaseous Acetic Acid Treatment on Breadmaking Properties of Flour     

Machiko Hayashi  Masaharu Seguchi 《Cereal Chemistry》1999,76(6):898-901

Eleven different wheat flours of varying protein content were treated with gaseous acetic acid. Each wheat flour exhibited maximum breadmaking properties (bread height and specific volume) at different levels of gaseous acetic acid treatment. There was a relationship (r = +0.7384) between the level of gaseous acetic acid treatment required for maximum breadmaking properties and the protein content of the wheat flour. Wheat flours with higher protein contents were more resistant to the decrease in pH value due to acetic acid treatment.  相似文献   

Analytical characterization of 2-bromo-palmitic acid by gas chromatography–mass spectrometry as a tool for analysis of lipid metabolism in fish     

Tokuda  Masaharu  Oku  Hiromi 《Fisheries Science》2009,75(2):533-535

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Zoogeographical barriers causing discontinuous osteometrical variations in the northern treeshrew skulls     

Hideki ENDO  Truong Son NGUYEN  Dinh Duy NGUYEN  Motoki SASAKI  Junpei KIMURA  Tatsuo OSHIDA  Masaya YAGO  Ngoc Tu LY  Thi Tham NGUYEN  Masaharu MOTOKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(10):1534

Morphological variation of the skull was examined in the northern treeshrew (Tupaia belangeri) from various localities across Southeast Asia. Through a multivariate analysis, the treeshrews from South Vietnam exhibited distinct morphological characteristics compared to other populations from Thailand and Laos, and Malaysia. The plots of the specimens of North Vietnam are not randomly mixed with Thailand plots segregation in the scatteregrams of canonical discriminant analysis. Since the skulls of the population from North Vietnam were morphologically similar to those form central Laos and northern and northeastern Thailand, the zoogeographical barrier effect of Mekong River was not clearly confirmed. The population of the Kanchanaburi in western Thailand is clearly smaller in size compared to the other populations. The southern border of the distribution of this species is determined by the Isthmus of Kra or Kangar-Pattani Line. In the northern treeshrew, which is distributed from southern China to Bangladesh and southern Thailand, we have detected osteometrical geographical variation driven by geography. These results indicate that the skull morphology in the Tupaia glis-belangeri complex distinctively differs in South Vietnam, western Thailand, and southern Thailand. The zoogeographical barrier and factor separating these districts are expected to clarify in the future.  相似文献