Effect of Post-IVF Developmental Kinetics on In Vitro Survival of Vitrified-warmed Domestic Cat Blastocysts     

T Tsujioka  C Otzdorff  J Braun  S Hochi 《Reproduction in domestic animals》2008,43(3):323-327

A limited number of reports is available on cryopreservation of in vitro fertilization (IVF)‐derived cat blastocysts. In the present study, IVF‐derived domestic cat embryos which reached the blastocyst stage either on day 6 or day 7 were cryopreserved by vitrification using Cryotop as a cryodevice. Fresh control and post‐warm surviving blastocysts were examined by differential cell staining with Hoechst 33342 and propidium iodide to determine total cell number and inner cell mass (ICM) ratio, and the post‐warm survival rate was determined by re‐expansion of the blastocoel during 24 h of in vitro culture. In fresh control, the mean number of total cells of day 7 blastocysts (61.4 cells) tended to be smaller than that of day 6 blastocysts (81.9 cells, p = 0.096). The post‐warm survival rates of day 6 and day 7 blastocysts were not statistically different (73.8%; 31 of 42 vs 66.7%; 18 of 27). There were no significant differences in the total cell number and ICM ratio between fresh control and vitrified blastocysts, although the ICM ratio of surviving day 7 blastocysts was significantly smaller than that of fresh controls (stained at day 8, 18.9% vs 28.9%, p < 0.05). These results indicate that IVF‐derived domestic cat embryos that reached the blastocyst stage earlier can survive the Cryotop vitrification without a reduction in the parameters studied.  相似文献   

Similarities and differences in meat consumption patterns in Asia and Pacific-Rim countries   总被引：1，自引：0，他引：1  

Seiichi KOIZUMI  Shinichi KOBAYASHI  In-Jen PAN  Shinichi TAKAKU  Masao BABA  Raymond A. JUSSAUME Jr    Paul RIETHMULLER  Julian MORISON  J. C. RYOO  C. W. LIN  I. H. LIN  H. ZHOU  Apichai RATANAWARAHA  Minoru NAGANO 《Animal Science Journal》2002,73(4):293-300

This study investigated consumer behavior towards meat across Asia and Pacific-Rim countries. Mail surveys were undertaken in 1994 and 1995. Data was obtained from 6197 households in Japan, the USA (mainland and Japanese–Americans in Hawaii), Australia, Thailand, Korea, Taiwan and China. The data were mainly analyzed using cluster analysis to determine the distance between each country and to classify countries into groups. Japanese respondents purchased beef more frequently than respondents from other countries. The average amount purchased on each occasion was far more for Australian respondents than for respondents from other countries, while Japanese purchased far less. Respondents in Japan, Korea, China and Australia thought beef was the most expensive meat, whereas lamb was thought to be the most expensive in the remaining countries. Chinese respondents indicated the highest consciousness of price indexes. The level of uneasiness about eating meat indicated by Asian respondents was relatively strong compared with respondents in Hawaii, the USA and Australia. Average ratings (semantic differential scale) on plans for future meat consumption were higher for beef than for other meats in Korea and China, whereas chicken was rated higher in the other countries. From the dendrogram, surveyed countries were classified into three groups: Japan, Taiwan and Korea; Thailand and China; and the USA, Japanese–Americans and Australia. Among them, the first and second clusters merged into a larger cluster, the 'Asian' group.  相似文献   

Mapping of expressed sequence tag markers with a cDNA-amplified fragment length polymorphism method in Japanese quail (Coturnix japonica)     

Shinji SASAZAKI  Tomoko HINENOYA  Daisuke FUJIMA  Shinichi KIKUCHI  Akira FUJIWARA  Hideyuki MANNEN 《Animal Science Journal》2006,77(1):42-46

In our previous study, a Kobe‐NIBS Japanese quail (KNQ) linkage map was constructed mainly using amplified fragment length polymorphism (AFLP) markers. In order to compare chicken and quail chromosomes, we developed expressed sequence tag (EST) markers derived from cDNA‐AFLP fragments and localized these markers on the linkage map. Using a total of 128 AFLP primer combinations, 24 polymorphic bands were obtained between a neurofilament‐deficient mutant quail line male and a muscular disorder quail line female, which were the parents of the KNQ resource family. Nine of the 24 markers were mapped by linkage analysis. These markers were mapped to seven linkage groups, namely 1, 2, 3, 6, 8, 15 and 42. A subsequent homology search using chicken genome sequences strongly suggests that these linkage groups correspond with chicken chromosomes 1, 2, 3, 5, 15, 23 and 26.  相似文献   

Effect of Leukemia Inhibitory Factor and Forskolin on Establishment of Rat Embryonic Stem Cell Lines     

Masumi HIRABAYASHI  Teppei GOTO  Chihiro TAMURA  Makoto SANBO  Hiromasa HARA  Shinichi HOCHI 《The Journal of reproduction and development》2014,60(1):78-82

This study was designed to investigate whether supplementation of 2i medium with leukemia inhibitory factor (LIF) and/or forskolin would support establishment of germline-competent rat embryonic stem (ES) cell lines. Due to the higher likelihood of outgrowth rates, supplementation of forskolin with or without LIF contributed to the higher establishment efficiency of ES cell lines in the WDB strain. Germline transmission competency of the chimeric rats was not influenced by the profile of ES cell lines until their establishment. When the LIF/forskolin-supplemented 2i medium was used, the rat strain used as the blastocyst donor, such as the WI strain, was a possible factor negatively influencing the establishment efficiency of ES cell lines. Once ES cell lines were established, all lines were found to be germline-competent by a progeny test in chimeric rats. In conclusion, both LIF and forskolin are not essential but can play a beneficial role in the establishment of “genuine” rat ES cell lines.  相似文献   

The Effects of Calcitonin on the Development of and Ca2+ Levels in Heat-shocked Bovine Preimplantation Embryos In Vitro     

Shumpei KAMANO  Shuntaro IKEDA  Miki SUGIMOTO  Shinichi KUME 《The Journal of reproduction and development》2014,60(4):317-323

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Influences of protein ingestion on glucagon‐like peptide (GLP)‐1‐immunoreactive endocrine cells in the chicken ileum     

Mohammad M. Monir  Kohzy Hiramatsu  Sachiko Matsumoto  Kei Nishimura  Chihiro Takemoto  Tomomi Shioji  Takafumi Watanabe  Kazumi Kita  Shinichi Yonekura  Sang Gun Roh 《Animal Science Journal》2014,85(5):581-587

Influences of a specific dietary nutrient on glucagon‐like peptide (GLP)‐1‐containing cells in the chicken intestine are not yet clear. Significance of dietary protein level on GLP‐1‐containing cells in the chicken ileum was investigated. Chickens fed control or experimental diets of varying protein levels were examined using immunohistochemical and morphometrical techniques. We show that the protein ingestion had an impact on the activities of GLP‐1‐immunoreactive cells in the chicken ileum. Weight gains declined with decreasing dietary crude protein (CP) levels, but no significant differences were detected in the daily feed intake and villous height. GLP‐1‐immunoreactive cells with a round or oval shape were frequently observed in the lower CP level groups (4.5% and 0%). Frequencies of occurrence of GLP‐1‐immunoreactive cells were 41.1 ± 4.1, 38.5 ± 4, 34.8 ± 3.1 and 34.3 ± 3.7 (cells/mm², mean ± SD) for dietary CP level of 18%, 9%, 4.5% and 0% groups, respectively and significant differences were recognized between the control and lower CP level groups (P < 0.05). Multiple regression analysis indicated a significant correlation between the daily protein intake and frequencies of occurrence of GLP‐1‐immunoreactive cells. The protein ingestion is one of the signals that influence GLP‐1‐containing cells in the chicken small intestine.  相似文献   

Induction of (<Emphasis Type="Italic">Z</Emphasis>)-8-heptadecene in marine green alga <Emphasis Type="Italic">Bryopsis maxima</Emphasis> by mechanical wounding     

Yoshihiko Akakabe  Soota Iwamoto  Shinichi Miyamura  Tadahiko Kajiwara 《Fisheries Science》2007,73(5):1057-1060

ABSTRACT:   After mechanical wounding of the marine green alga Bryopsis maxima in a phosphate buffer, one main organic component was released to the solution. The organic compound was characterized by a molecular ion at 238  m/z by gas chromatography–mass spectrometry (GC-MS) and was deduced as 8-heptadecene by the fragment pattern. To identify the stereochemistry of 8-heptadecene, synthetic ( Z / E )-8-heptadecene was coinjected to the solution of wounded B. maxima . Comparing retention times and mass spectra with the synthetic sample, the target hydrocarbon obtained from wounded B. maxima was coincident with ( Z )-8-heptadecene.  相似文献   

Isolation of an equine coronavirus from adult horses with pyrogenic and enteric disease and its antigenic and genomic characterization in comparison with the NC99 strain     

Oue Y  Ishihara R  Edamatsu H  Morita Y  Yoshida M  Yoshima M  Hatama S  Murakami K  Kanno T 《Veterinary microbiology》2011,150(1-2):41-48

A new equine coronavirus was isolated from the feces of adult horses with pyrogenic and enteric disease. The disease outbreak was mainly observed among 2- to 4-year-old horses living in stables of a draft-horse racetrack in Japan. On comparing the isolated virus (isolate Tokachi09) with the equine coronavirus NC99 strain, no significant differences were observed in several biological properties such as hemagglutinating activity, antigenicity (in indirect immunofluorescence and neutralization tests), and one-step growth (in cell culture). The sequences of the nucleocapsid and spike genes of isolate Tokachi09 showed identical size (1341 and 4092 nucleotides, 446 and 1363 amino acids, respectively) and high similarity (98.0% and 99.0% at the nucleotides, 97.3% and 99.0% at the amino acids, respectively) to those of strain NC99. However, the isolate had a 185-nucleotide deletion from four bases after the 3'-terminal end of the spike gene, resulting in the absence of the open reading frame predicted to encode a 4.7-kDa nonstructural protein in strain NC99. These results suggest that the 4.7-kDa nonstructural protein is not essential for viral replication, at least in cell culture, and that the Japanese strain probably originated from a different lineage to the North American strain. This is the first equine coronavirus to be isolated from adult horses with pyrogenic and enteric disease.  相似文献   

Isolation of a Megatrypanum trypanosome from sika deer (Cervus nippon yesoensis) in Japan     

Hatama S  Shibahara T  Suzuki M  Kadota K  Uchida I  Kanno T 《Veterinary parasitology》2007,149(1-2):56-64

A trypanosome was isolated from a sika deer (Cervus nippon yesoensis) in Hokkaido, Japan, during the primary culture of sika deer renal cells. This is the first report of isolation of a Megatrypanum trypanosome from Japanese Cervidae. The trypanosome, designated TSD1, was propagated and maintained in Eagle's modified essential medium containing 20% fetal bovine serum with sika deer renal cells as feeder. The TSD1 trypanosome was morphometrically similar to Trypanosoma cervi, which is commonly isolated from American and European deer. PCR analysis with primers for 18S ribosomal DNA and nucleotide sequencing showed that TSD1 is a member of genus Trypanosoma, subgenus Megatrypanum. Phylogenetically TSD1 is closely related to T. theileri, a common trypanosome of cattle, but is distinguishable from T. theileri by some morphometrical and biological features.  相似文献   

Vertebral stabilization using positively threaded profile pins and polymethylmethacrylate, with or without laminectomy, for spinal canal stenosis and vertebral instability caused by congenital thoracic vertebral anomalies     

Aikawa T  Kanazono S  Yoshigae Y  Sharp NJ  Muñana KR 《Veterinary surgery : VS》2007,36(5):432-441

Objective— To describe diagnostic findings, surgical technique, and outcome in dogs with thoracic spinal canal stenosis and vertebral instability secondary to congenital vertebral anomalies.
Study Design— Retrospective clinical study.
Animals— Dogs (n=9) with thoracic spinal canal stenosis.
Methods— Medical records (1995–1996; 2000–2006) of 9 dogs with a myelographic diagnosis of spinal canal stenosis and/or vertebral instability secondary to congenital vertebral anomaly that were surgically managed by vertebral stabilization with or without laminectomy were reviewed. Data on pre- and postoperative neurologic status, diagnostic findings, surgical techniques, and outcomes were retrieved. Follow-up evaluations were performed at 1, 2, and 6 months. Long-term outcome was assessed by means of clinical examination or owner telephone interviews.
Results— Spinal cord compression was confirmed by myelography, and in 2 dogs, dynamic compression by stress myelography. Eight dogs regained the ability to ambulate postoperatively. One dog with a partial recovery regained voluntary movement but did not become ambulatory.
Conclusions— Spinal cord injury secondary to congenital vertebral anomaly may have a good outcome when treated by vertebral stabilization with or without laminectomy. Adequate stabilization of the vertebrae and improved neurologic outcome were achieved in most dogs.
Clinical Relevance— Vertebral stabilization using positively threaded profile pins and polymethylmethacrylate with or without laminectomy is an effective treatment for spinal canal stenosis and vertebral instability secondary to congenital thoracic vertebral anomalies.  相似文献