全文获取类型
收费全文 | 228篇 |
免费 | 5篇 |
专业分类
林业 | 7篇 |
农学 | 4篇 |
基础科学 | 1篇 |
39篇 | |
综合类 | 19篇 |
农作物 | 35篇 |
水产渔业 | 19篇 |
畜牧兽医 | 98篇 |
园艺 | 3篇 |
植物保护 | 8篇 |
出版年
2021年 | 2篇 |
2020年 | 5篇 |
2019年 | 7篇 |
2018年 | 5篇 |
2016年 | 5篇 |
2015年 | 3篇 |
2014年 | 7篇 |
2013年 | 13篇 |
2012年 | 5篇 |
2011年 | 11篇 |
2010年 | 4篇 |
2009年 | 3篇 |
2008年 | 7篇 |
2007年 | 8篇 |
2006年 | 12篇 |
2005年 | 10篇 |
2004年 | 15篇 |
2003年 | 14篇 |
2002年 | 3篇 |
2001年 | 9篇 |
2000年 | 6篇 |
1999年 | 3篇 |
1998年 | 5篇 |
1997年 | 7篇 |
1996年 | 3篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 11篇 |
1990年 | 3篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1975年 | 2篇 |
1974年 | 1篇 |
1971年 | 1篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1963年 | 1篇 |
1960年 | 1篇 |
1932年 | 1篇 |
1931年 | 1篇 |
1928年 | 1篇 |
排序方式: 共有233条查询结果,搜索用时 46 毫秒
1.
2.
C-reactive protein concentrations in canine acute pancreatitis 总被引:1,自引:0,他引:1
Jennifer L. Holm DVM Elizabeth A. Rozanski DVM DACVECC DACVIM Lisa M. Freeman DVM PhD DACVN Cynthia R. L. Webster DVM DACVIM 《Journal of Veterinary Emergency and Critical Care》2004,14(3):183-186
Objective: To determine if C‐reactive protein (CRP) concentration is elevated in spontaneously occurring canine acute pancreatitis (AP), and to measure changes in CRP during the course of hospitalization. Design: Prospective study. Setting: Tufts University School of Veterinary Medicine Foster Hospital for Small Animals. Animals: Sixteen client‐owned dogs with AP and 16 healthy controls. Interventions: Blood samples were obtained from the AP group on the day of diagnosis (Day 1), and on Days 3 and 5, unless the dog died or was discharged from the hospital. Blood was obtained from the control dogs once. Measurements and main results: Serum CRP was measured using a commercial immunoassay for each dog with AP and for healthy controls. Day 1 CRP concentrations were significantly higher in the AP group (56.1±12.7 μg/mL) compared with controls (2.8±1.3 μg/mL; P<0.001). For the 7 dogs that had samples collected on all 3 days, the mean CRP concentrations decreased significantly (P=0.043) over the 5 days of measurement. Of the 16 dogs with AP, 14 were discharged from the hospital and 2 were euthanized. Conclusions: Serum CRP concentrations were elevated in this group of 16 dogs with spontaneously occurring AP. In the 7 dogs that had measurements on all 3 days, the mean CRP concentration decreased from the day of diagnosis to the measurement made 5 days later. 相似文献
3.
Marine Rolland Bodil K. Larsen Jørgen Holm Johanne Dalsgaard Peter V. Skov 《Aquaculture International》2015,23(4):1071-1087
4.
Hormone-sensitive lipase: sequence, expression, and chromosomal localization to 19 cent-q13.3 总被引:17,自引:0,他引:17
C Holm T G Kirchgessner K L Svenson G Fredrikson S Nilsson C G Miller J E Shively C Heinzmann R S Sparkes T Mohandas 《Science (New York, N.Y.)》1988,241(4872):1503-1506
Hormone-sensitive lipase, a key enzyme in fatty acid mobilization, overall energy homeostasis, and possibly steroidogenesis, is acutely controlled through reversible phosphorylation by catecholamines and insulin. The 757-amino acid sequence predicted from a cloned rat adipocyte complementary DNA showed no homology with any other known lipase or protein. The activity-controlling phosphorylation site was localized to Ser563 in a markedly hydrophilic domain, and a lipid-binding consensus site was tentatively identified. One or several messenger RNA species (3.3, 3.5, or 3.9 kilobases) were expressed in adipose and steroidogenic tissues and heart and skeletal muscle. The human hormone-sensitive lipase gene mapped to chromosome 19 cent-q13.3. 相似文献
5.
The modern crop scientist has a large amount of available nucleotide sequence information to identify genes of potential agronomic importance.…… 《分子植物育种》2007,5(2):238-239
The modem crop scientist has a large amount of available nucleotide sequence information to identify genes of potential agronomic importance. Using reverse genetic approaches, specific genes can be disrupted, and hypotheses regarding gene function directly tested in vivo. Although a number of reverse genetic methods have been introduced, many are limited in application because they are organism-specific, expensive, transgenic, or only transiently disrupt gene function. However, traditional mutagenesis using chemical mutagens has been widely used as a forward genetics strategy to create many new crop plant varieties at relatively low cost. Mutagens such as ethyl methanesulphonate (EMS) cause stable point mutations and thus produce an allelic series of truncation and missense changes that can provide a range of phenotypes (Greene et al., 2003). TILLING (targeting induced local lesions IN genomes) is a high-throughput reverse genetic strategy that combines traditional mutagenesis and SNP discovery methods (Colbert et al., 2001; McCallum et al., 2000). To identify mutations, target regions of-l.5 kb are amplified with fluorescently labeled gene specific primers. Heteroduplexes are then formed between wild-type and mutant strands, mismatches are cleaved by incubation with a single-strand specific nuclease, 相似文献
6.
Venugopal CS Holmes EP Koch CE Curtis LA Holm AS Moore RM 《American journal of veterinary research》2001,62(2):154-159
OBJECTIVE: To evaluate the effectiveness of 2 potential endothelin (ET)-1 antagonists in blocking the contractile responses of equine colonic vessels to increasing concentrations of ET-1. SAMPLE POPULATION: Mesenteric vessels from 6 clinically healthy horses. PROCEDURE: Colonic vessels (arterial and venous rings) were placed in organ baths with oxygenated Tyrode solution at 37 C. Each was attached to a force transducer interfaced with a polygraph, and 2 g of tension was applied and equilibrated for 45 minutes. Then, B-1 (PD 142893) and B-2 (PD 145065) ET-1 antagonists were tested. One ring from each vessel type was used as a control for determining concentration-response relationships of ET-1 (10(-10) to 10(-6)M). Three rings of each vessel type were incubated with 3 concentrations of each antagonist (10(-7), 10(-6), and 10(-5) M) for 30 minutes before ET induced contractions were determined. The maximum contractile response and pA2 values were determined. RESULTS: Vessels contracted in a concentration-dependent manner to ET-1. Arteries responded slowly but reached greater contractions. Veins responded immediately with sustained contractions. Both antagonists inhibited contractions in a concentration-dependent manner with significant differences at 10(-6) and 10(-5)M for arteries and 10(-5) M for veins. Complete blockade of contractions was observed with B-2 (10(-5)M). The pA2 values for B-1 were 8.26 and 6.82 for arteries and veins, respectively, whereas they were 8.25 and 7.21 for B-2. CONCLUSION AND CLINICAL RELEVANCE: Both antagonists effectively blocked ET-1-induced contractions of equine colonic vessels. Because B-2 is water soluble and caused complete blockade at 10(-5) M, it appears to be the preferred antagonist. 相似文献
7.
8.
Holm BR 《Veterinary dermatology》2003,14(4):189-195
Ninety-nine dogs diagnosed with generalized demodicosis were treated with milbemycin oxime. Cases diagnosed before two years of age were classified as juvenile-onset (53%) and those diagnosed after two years of age as adult-onset (47%). Dogs were considered cleared of mites when none could be demonstrated in scrapings and cured if no relapse was seen for 12 months. Eighty-five per cent (84/99) were cured with milbemycin oxime used for 1-6 months (mean 2.3 months) at a dosage of 0.5-1.6 mg kg-1 body weight (mean 0.75 mg kg-1). No significant difference in dosage or treatment time was seen between juvenile and adult cases. Chance of cure was significantly better in young animals (cured cases mean age 2.97 years) than in older animals (not cured cases mean age 8.02 years). Start of treatment early in the course of disease gave a significantly better chance of cure. Cases with severe pododemodicosis had less chance of a cure (9/11 not cured). 相似文献
9.
Top 20 environmental weeds for classical biological control in Europe: a review of opportunities, regulations and other barriers to adoption 总被引:4,自引:3,他引:4
Classical biological control remains the only tool available for permanent ecological and economic management of invasive alien species that flourish through absence of their co‐evolved natural enemies. As such, this approach is recognized as a key tool for alien species management by the Convention on Biological Diversity (CBD), the European and Mediterranean Plant Protection Organization (EPPO) and the European Strategy on Invasive Alien Species (ESIAS). Successful classical biological control programmes abound around the world, despite disproportionate attention being given to occasional and predictable non‐target impacts. Despite more than 130 case histories in Europe against insect pests, no exotic classical biological control agent has been released in the EU against an alien invasive weed. This dearth has occurred in the face of increasing numbers of exotic invasive plants being imported and taking over National Parks, forests and amenity areas in this region, as well as a global increase in the use of classical biological control around the world. This paper reviews potential European weed targets for classical biological control from ecological and socioeconomic perspectives using the criteria of historical biological control success, taxonomic isolation from European native flora, likely availability of biological control agents, invasiveness outside Europe and value to primary industry and horticulture (potential for conflicts of interest). We also review why classical biological control of European exotic plants remains untested, considering problems of funding and public perception. Finally, we consider the regulatory framework that surrounds such biological control activities within constituent countries of the EU to suggest how this approach may be adopted in the future for managing invasive exotic weeds in Europe. 相似文献
10.