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91.
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OBJECTIVE: To evaluate duration of action of an insulin zinc suspension (Caninsulin, Intervet) in spontaneously occurring cases of canine diabetes mellitus and suitability of its use as a once daily administered insulin for treatment of this disease. DESIGN: Eight client-owned canine diabetics were included in a prospective pilot study. All dogs had been treated with Caninsulin for a minimum of 2 months and were considered on clinical grounds to be adequately stabilised. PROCEDURE: Dogs were hospitalised for 24 h and blood collected every 2 h via indwelling venous catheters for blood glucose determination. RESULTS: Once daily Caninsulin administration failed to maintain glycaemic control for greater than 13 h in five of eight dogs, but acceptable blood glucose concentrations were maintained for 22 h and greater than 24 h in two others. One dog became distressed during hospitalisation and the blood glucose curve did not show an identifiable response to the insulin. CONCLUSION: Most diabetic dogs may require twice daily administration of Caninsulin for satisfactory glycaemic control, but once daily administration may be adequate in some animals. More comprehensive investigation into duration of activity of Caninsulin is warranted.  相似文献   
94.
Laboratory compressibility tests were done on soil samples taken from a field experiment 21 years old, located on a Stagnic Luvisol, with deep ripping performed with various frequencies: no ripping, ripping every 8, 4 and 2 years, and ripping yearly. Precompression stress was found to increase with depth of the soil profile down to some 60 cm, and somewhat decreasing at the depth of 70–75 cm, which corresponds to the Bt horizon. Due to ripping, the values of the precompression stress decreased; for soils from experimental treatments with different periodicity of ripping operations, the differences were small, and not in a very definite direction. The estimation procedures suggested by Lebert to predict precompression stress for “normal” arable soils could not be applied to ameliorated soil samples investigated in this paper because repeated ripping prevents a continuous aggregate formation and results mainly in structural texture dependent relations. The compression index showed an increase down to 60 cm and a decrease in the Bt horizon (70–75 cm). In the different experimental treatments, it showed a less clear variation, although some trend of increasing with increased number of rippings may be considered. As inferred from these parameters, soil strength and compressibility do not affect directly crop yields.  相似文献   
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The aquands found in southern Chile are derived from volcanic ash and contain high levels of organic matter. Due to the presence of an impermeable stratum, they have shallow soil profiles, which induce waterlogging for several months each year. These fragile soils, locally known as ‘Ñadi’, cover an area of 475 000 hectares and have undergone intensive land use changes, which have affected the soil physical properties. These are still not well understood but are relevant for the design of efficient drainage systems. The aim of this research was to analyse the impact of the land use change in Ñadi soils on the spatial and temporal variability of their soil physical properties. For the land use change from secondary native forest (sNF) to naturalized grassland (NG), the effective soil depth was measured at defined points. Time‐ and space‐dependent changes of water‐table depth and penetration resistance were recorded. Volumetric water content and soil temperature were measured with sensors installed at three depths and the water retention curve and air permeability at these depths were also determined. The changes in land use over time have induced a reduction in soil depth. Soils under NG showed a smaller soil water storage capacity, air capacity and permeability compared with soils under sNF, as well as waterlogging during winter and greater mechanical strength and soil profile temperatures during summer. Therefore, the land use change affected the spatial and temporal variability of soil physical functions across the field.  相似文献   
97.
Oil palm plantations, irreversibly claimed primarily from tropical forest, carpet the landscape in Malaysia and Indonesia, the largest global producers of palm oil. The impact of forest conversion...  相似文献   
98.
Avian osteoblasts have been isolated particularly from chicken embryo, but data about other functional tissue sources of adult avian osteoblast precursors are missing. The method of preparation of pigeon osteoblasts is described in this study. We demonstrate that pigeon cancellous bone derived osteoblasts have particular proliferative capacity in vitro in comparison to mammalian species and developed endogenous ALP. Calcium deposits formation in vitro was confirmed by alizarin red staining. Only a few studies have attempted to investigate bone grafting and treatment of bone loss in birds. Lack of autologous bone grafts in birds has prompted investigation into the use of avian xenografts for bone augmentation. Here we present a method of xenografting of ostrich demineralised cancellous bone scaffold seeded with allogeneic adult pigeon osteoblasts. Ostrich demineralised cancellous bone scaffold supported proliferation of pigeon osteoblasts during two weeks of co - cultivation in vitro. Scanning electron microscopy demonstrated homogeneous adult pigeon osteoblasts attachment and distribution on the surface of xenogeneic ostrich demineralised cancellous bone. Our preliminary in vitro results indicate that demineralised cancellous bone from ostrich tibia could provide an effective biological support for growth and proliferation of allogeneic osteoblasts derived from cancellous bone of pigeons.  相似文献   
99.
Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia. The first involves the primer-mediated enzymatic amplification of specific beta-globin target sequences in genomic DNA, resulting in the exponential increase (220,000 times) of target DNA copies. In the second technique, the presence of the beta A and beta S alleles is determined by restriction endonuclease digestion of an end-labeled oligonucleotide probe hybridized in solution to the amplified beta-globin sequences. The beta-globin genotype can be determined in less than 1 day on samples containing significantly less than 1 microgram of genomic DNA.  相似文献   
100.
During the passage of Voyager 2 through the Saturn system, infrared spectral and radiometric data were obtained for Saturn, Titan, Enceladus, Tethys, Iapetus, and the rings. Combined Voyager 1 and Voyager 2 observations of temperatures in the upper troposphere of Saturn indicate a seasonal asymmetry between the northern and southern hemispheres, with superposed small-scale meridional gradients. Comparison of high spatial resolution data from the two hemispheres poleward of 60 degrees latitude suggests an approximate symmetry in the small-scale structure, consistent with the extension of a symmetric system of zonal jets into the polar regions. Longitudinal variations of 1 to 2 K are observed. Disk- averaged infrared spectra of Titan show little change over the 9-month interval between Voyager encounters. By combining Voyager 2 temperature measurements with ground-based geometric albedo determinations, phase integrals of 0.91 +/- 0.13 and 0.89 +/- 0.09 were derived for Tethys and Enceladus, respectively. The subsolar point temperature of dark material on Iapetus must exceed 110 K. Temperatures (and infrared optical depths) for the A and C rings and for the Cassini division are 69 +/- 1 K (0.40 +/- 0.05), 85 +/- 1 K (0.10 +/- 0.03), and 85 +/- 2 K (0.07 +/- 0.04), respectively.  相似文献   
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