In Vivo and In Vitro Sperm Interaction with Oviductal Epithelial Cells of Llama     

S Apichela  MA Jiménez‐Díaz  M Roldan‐Olarte  JN Valz‐Gianinet  DC Miceli 《Reproduction in domestic animals》2009,44(6):943-951

Sperm reservoirs in South American Camelids would be crucial for successful fertilization. Since ovulation occurs approximately 36 h after mating, the maintenance of the sperm viability in the oviduct waiting for the ovum is a critical reproductive event. Our study aimed at determining whether the isthmus or the utero tubal junction (UTJ) could function as a sperm reservoir in llama by means of in vivo and in vitro experiments. For the in vivo experiments, the oviducts of adult females with a dominant follicle bigger than 7 mm were examined for the presence of sperm at 6, 18, 24, 28 and 35 h after mating. The results using scanning and transmission electron microscopy showed ultrastructural differences between isthmus and UTJ with respect to (1) predominance of secretory cells in the UTJ and ciliated cells in the isthmus epithelium and (2) cytoplasmic bulbous projection of the secretory cells in the UTJ. Sperm adhered by a mucus‐like substance were seen only in the UTJ at 6, 18, 24 and 28 h post‐mating. Lack of sperm adhered to oviductal mucosa was observed around ovulation (35 h). In vitro experiments demonstrated higher ability of UTJ epithelial cell explants with respect to isthmus explants to bind sperm in a co‐cultured system. The anatomical features and the presence of a sperm bonding agent in the UTJ together with the in vitro differential binding of sperm to UTJ explants strongly suggest that both may be feasible mechanisms that facilitate sperm storage in this oviductal region in llama.  相似文献   

Book Review: Countryside in trust: land management by conservation,recreation and amentity organisations,Janet C. Dwyer and Ian D. Hodge; John Wiley & Sons Chichester, 1996. Hardback, 314 pp. Price: £25.00     

Thomas Huxley 《水产资源保护:海洋与淡水生态系统》1996,6(3):191-191

  相似文献   

THE CONNECTION OF THE BIOLOGICAL SCIENCES WITH MEDICINE     

Huxley TH 《Science (New York, N.Y.)》1881,2(64):426-429

  相似文献   

Prothrombin time and activated partial thromboplastin time using a point‐of‐care analyser (Abaxis VSpro®) in Bennett's wallabies (Macropus rufogriseus)          下载免费PDF全文

BN Nevitt  SK Chinnadurai  MK Watson  JN Langan  MJ Adkesson 《Australian veterinary journal》2016,94(10):384-386

There are few reports of coagulation times in marsupial species. Blood samples collected from 14 Bennett's wallabies (Macropus rufogriseus) under anaesthesia during routine health assessments were analysed for prothrombin time (PT) and activated partial thromboplastin time (aPTT) using a point‐of‐care analyser (POC) (Abaxis VSPro®). The wallabies had an aPTT mean of 78.09 s and median of 78.1 s. The PT for all wallabies was greater than 35 s, exceeding the longest time measured on the POC. Although PT was significantly longer, aPTT was similar to the manufacturer's domestic canine reference range.  相似文献   

Molecular detection of Bartonella coopersplainsensis and B. henselae in rats from New Zealand     

JN Vijayan Genitha Helan  K Gedye  MA Potter  S Harrus 《New Zealand veterinary journal》2018,66(5):257-260

  相似文献   

INTERSEXES IN DROSOPHILA AND DIFFERENT TYPES OF INTERSEXUALITY     

Huxley JS 《Science (New York, N.Y.)》1920,52(1333):59-60

  相似文献   

The tree/crop interface — or simplifying the biological/environmental study of mixed cropping agroforestry systems     

Peter A. Huxley 《Agroforestry Systems》1985,3(3):251-266

A key to understanding the biological potentials and restraints of agroforestry systems, and the environmental responses of plant components within them, is the tree/crop interface. All agroforestry systems can be studied by separating the growth and yield characteristics of the three basic sets of variables (a) the sole agricultural crop (b) the effects of the tree/crop interface on the crop and tree and (c) the growth of the tree as a whole crop.The interface can be studied wherever it occurs in natural situations, or conventional or systematic experimental layouts, but very simple forms of geometric layouts can be very space efficient.

---

Resumer La interfase arbol/cultivo, o como simplificar los estodios biologicoambientales de sistemas mixtos de cultivos agrogorestales.La interface arbol/cultivo es la clave para entender el potencial y las limitaciones biologicas de los sistemas agroforestales, y de la respuesta ambiental de las plantas dentro de los mismos. Todos los sistemas agroforestales pueden ser estudiados separando las caracteristicas de crecimiento y rendimiento de los tres conjuntos basicos de variables: (a) el cultivo agricola solo; (b) los efectos de la interfase arbol/cultivo sobre el cultivo y los arboles; y (c) la plantation forestal sola.La interfase puede ser estudiada cuando ella ocurre en situaciones naturales, o en arreglos experimentales convencionales o sistematicos, pero formas simples de arreglos geometricos pueden ser muy eficientes en el uso del espacio.

  相似文献   

ZOOLOGICAL SOCIETY OF LONDON     

Huxley JS 《Science (New York, N.Y.)》1940,92(2377):60

  相似文献   

Impact of Probing the Reproductive Tract During Early Pregnancy on Fertility of Beef Cows     

G Pugliesi  SC Scolari  FS Mesquita  M Maturana Filho  ER Araújo  D Cardoso  JN Sales  I Martin  M Sá Filho  CM Bertan  M Binelli 《Reproduction in domestic animals》2014,49(4):e35-e39

This short communication reports the impact of endometrial biopsies, uterine flushings and follicular fluid aspiration procedures at day 6 post artificial insemination (AI) on pregnancy rates. In Experiment 1, cows were timed AI (TAI) and assigned to the following treatment groups: control (n = 37), uterine flushing (n = 35) and endometrial biopsy (n = 38). On day 30 post AI, pregnancy rates were 40.5%, 33% and 28.5%, respectively (p > 0.1). Pregnancy rate on day 60 was lower (p < 0.004) in flushed cows than in the controls. In Experiment 2, oestrus was detected and cows were assigned to flushing (n = 32) or biopsy (n = 33) treatments 6 days after AI, which resulted in pregnancy rates of 31% and 36%, respectively (p > 0.1). In Experiment 3, cows were, 6 days after TAI, randomly assigned to the following treatments: control (n = 84) or aspiration of the largest follicle (n = 73). Pregnancy rates on day 30 post AI were 63.5% for the control group and 53% for the aspirated group (p > 0.1). In conclusion, uterine flushing and endometrial biopsy negatively affect pregnancy rates, but neither procedure can be considered to be incompatible with pregnancy maintenance. Follicular aspiration during pregnancy does not interact with pregnancy success. The amount and quality of samples obtained are compatible with the use of cellular and molecular analysis of uterine variables from cows that failed or succeeded on maintaining pregnancy.  相似文献   

SCIENCE,WAR AND RECONSTRUCTION     

Huxley JS 《Science (New York, N.Y.)》1940,91(2355):151-158

  相似文献